##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935125.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723034
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.030957050429166	33.0	31.0	34.0	30.0	34.0
2	32.3633245462869	34.0	31.0	34.0	30.0	34.0
3	32.40881341679644	34.0	31.0	34.0	30.0	34.0
4	35.92134809704661	37.0	35.0	37.0	35.0	37.0
5	30.3976604143097	37.0	35.0	37.0	0.0	37.0
6	32.87318991914626	37.0	35.0	37.0	17.0	37.0
7	35.07464932492801	37.0	35.0	37.0	32.0	37.0
8	35.62526520191305	37.0	35.0	37.0	35.0	37.0
9	37.503620853237884	39.0	37.0	39.0	35.0	39.0
10	37.43041267768874	39.0	37.0	39.0	35.0	39.0
11	37.278801550134574	39.0	37.0	39.0	34.0	39.0
12	37.04975423009153	39.0	37.0	39.0	34.0	39.0
13	36.92326916853149	39.0	37.0	39.0	33.0	39.0
14	37.90658253968693	40.0	37.0	41.0	33.0	41.0
15	38.10691060171444	40.0	37.0	41.0	33.0	41.0
16	38.24398022776246	40.0	37.0	41.0	34.0	41.0
17	38.27415169964345	40.0	37.0	41.0	34.0	41.0
18	38.21597047994977	40.0	37.0	41.0	34.0	41.0
19	38.213007963664225	40.0	37.0	41.0	34.0	41.0
20	38.1231975259808	40.0	36.0	41.0	34.0	41.0
21	38.08246500164584	40.0	36.0	41.0	34.0	41.0
22	38.09183385566931	40.0	36.0	41.0	34.0	41.0
23	38.04276700680742	40.0	36.0	41.0	34.0	41.0
24	38.02940249006271	40.0	36.0	41.0	34.0	41.0
25	37.9655728499628	40.0	36.0	41.0	34.0	41.0
26	37.90482190325766	40.0	36.0	41.0	34.0	41.0
27	37.832775498800885	40.0	36.0	41.0	34.0	41.0
28	37.797710757723706	40.0	36.0	41.0	34.0	41.0
29	37.77863696589649	40.0	36.0	41.0	34.0	41.0
30	37.632553932456844	40.0	36.0	41.0	33.0	41.0
31	33.029951565209934	38.0	33.0	41.0	9.0	41.0
32	33.94490162288357	38.0	33.0	41.0	16.0	41.0
33	35.900198330922194	38.0	34.0	41.0	29.0	41.0
34	36.74402033652636	38.0	35.0	41.0	31.0	41.0
35	36.93064641496804	39.0	35.0	41.0	32.0	41.0
36	37.09072049170578	39.0	35.0	41.0	32.0	41.0
37	37.173431678178346	39.0	35.0	41.0	32.0	41.0
38	37.17535551578487	39.0	35.0	41.0	33.0	41.0
39	37.18200527222786	39.0	35.0	41.0	33.0	41.0
40	37.11945772951203	39.0	35.0	41.0	33.0	41.0
41	37.05139592328992	39.0	35.0	41.0	32.0	41.0
42	37.02174448227884	39.0	35.0	41.0	32.0	41.0
43	37.01401455533212	39.0	35.0	41.0	32.0	41.0
44	36.952182055062416	39.0	35.0	41.0	32.0	41.0
45	36.96068787913155	39.0	35.0	41.0	32.0	41.0
46	36.91794715047978	39.0	35.0	41.0	32.0	41.0
47	36.83893565171209	39.0	35.0	41.0	32.0	41.0
48	36.83364267793769	39.0	35.0	41.0	32.0	41.0
49	36.817531402396014	39.0	35.0	41.0	32.0	41.0
50	36.65052957398961	39.0	35.0	41.0	32.0	41.0
51	36.02827529549094	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	8.0
11	15.0
12	8.0
13	16.0
14	18.0
15	26.0
16	42.0
17	91.0
18	147.0
19	265.0
20	448.0
21	740.0
22	1241.0
23	1960.0
24	2815.0
25	3565.0
26	4337.0
27	5208.0
28	6419.0
29	8204.0
30	10852.0
31	15912.0
32	24383.0
33	28164.0
34	54430.0
35	70925.0
36	80755.0
37	80266.0
38	121515.0
39	200078.0
40	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1554173109425	21.51696877325271	23.848671016853977	12.478942898950809
2	32.380496629480774	23.889471311169324	25.044050487252328	18.685981572097575
3	32.45670328089689	23.93856997043016	25.511939964095742	18.09278678457721
4	28.207387204474475	25.833778217898466	26.013714431133252	19.945120146493803
5	21.54269370458374	39.6429490176119	21.378662690827817	17.435694586976545
6	28.003385732897762	31.2799951316259	24.629270546060074	16.087348589416266
7	77.9613406838406	6.238710766022068	9.727896613437267	6.072051936700072
8	77.15003720433617	5.679954193025501	10.19412641729158	6.975882185346747
9	71.94627085309958	7.500200543819516	11.904142820392956	8.649385782687952
10	41.29916435464999	24.7910886624972	19.145434377913073	14.76431260493974
11	31.073780762730387	24.973652691298057	24.772693953534688	19.17987259243687
12	29.167922946915358	21.884033116008375	29.293228257592315	19.654815679483953
13	25.092457616101036	24.987483299540546	30.354450828038516	19.565608256319898
14	18.572155666261892	28.451774052119266	31.792695779174977	21.18337450244387
15	17.79971619591886	27.153633162479217	35.07746523676618	19.969185404835734
16	22.496314142903376	25.529228224398853	30.692470893484952	21.281986739212815
17	22.229660015988184	25.610690506947115	27.628991167773577	24.530658309291127
18	22.30019611802488	26.573715758871643	29.30415443810388	21.821933684999596
19	23.074295261357	28.19493965705624	27.372018466628123	21.358746614958633
20	23.3370768179643	29.966917185083965	27.143813430627052	19.552192566324685
21	22.54472127175209	27.48888710627716	31.243758938030574	18.722632683940173
22	20.294204698534234	25.306002207365076	29.693762672294806	24.70603042180589
23	19.741118674917086	26.52724491517688	30.703811992243796	23.02782441766224
24	22.20836087929475	27.084756733431625	27.88665539933115	22.820226987942476
25	21.367598204233825	27.33052664190066	27.465928296594626	23.83594685727089
26	18.646149420359208	28.538768577964525	27.934785916015016	24.880296085661254
27	21.86148922457312	27.92510449024527	29.036117250364434	21.177289034817175
28	19.4311747442029	26.802059100955145	29.677995778898364	24.088770375943593
29	19.49106127789288	26.823634849813427	31.04542801583328	22.639875856460414
30	23.2120481194522	28.25911367930139	26.77011039591499	21.758727805331425
31	24.279356157525093	28.73059911428785	24.804504352492415	22.185540375694643
32	22.729636503954172	27.45126785185759	26.707042822329242	23.112052821859
33	22.429788917257003	26.069313476268057	26.16612773396548	25.334769872509455
34	20.07485125180835	26.011639839896876	29.250906596370296	24.662602311924473
35	19.219013213763112	26.23555738734278	28.361598486378238	26.18383091251587
36	21.937695875989235	26.09407026502212	26.027130121128465	25.941103737860182
37	20.539559688755993	28.96419808750349	27.561636105632655	22.93460611810786
38	21.621389865483504	26.22476951291364	26.603451566592994	25.55038905500986
39	21.496776085218674	24.095547373982413	27.61695853860261	26.7907180021963
40	21.950558341654748	24.826909937845247	28.751483332734008	24.471048387765997
41	18.797594580614465	25.595615143962803	26.463624117261432	29.143166158161304
42	20.70455884508889	24.581831559788338	28.868490278465465	25.845119316657307
43	21.973378845254857	24.30259157937248	28.960878741525292	24.763150833847373
44	21.829125601285693	24.242290127435222	28.236569787866127	25.69201448341295
45	20.75172121919578	23.793625196048872	27.503547551014197	27.95110603374115
46	21.911279414246078	24.589991618651403	27.763977904220273	25.734751062882243
47	18.38502753674101	24.88043439174368	31.796015125153176	24.938522946362134
48	19.756470650066248	23.928197014248294	29.46320643289251	26.85212590279295
49	21.404525928241274	21.962590970825715	31.22771543246929	25.405167668463726
50	19.80197335118404	23.216473914089793	29.749223411347188	27.232329323378984
51	19.26493083312818	23.83995773366121	26.551863397848514	30.343248035362098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	838.0
1	1057.0
2	1276.0
3	1443.0
4	1610.0
5	1376.0
6	1142.0
7	1167.0
8	1192.0
9	1264.0
10	1336.0
11	1440.5
12	1545.0
13	1609.5
14	1674.0
15	1783.5
16	1893.0
17	1886.5
18	1880.0
19	1967.5
20	2055.0
21	2203.0
22	2351.0
23	2408.5
24	2466.0
25	3122.0
26	4985.5
27	6193.0
28	7257.5
29	8322.0
30	9451.5
31	10581.0
32	12034.5
33	13488.0
34	15172.5
35	16857.0
36	18466.5
37	20076.0
38	22079.5
39	24083.0
40	26748.5
41	29414.0
42	34818.5
43	40223.0
44	46803.5
45	53384.0
46	60183.5
47	66983.0
48	75990.5
49	84998.0
50	84148.0
51	83298.0
52	69684.5
53	56071.0
54	49856.5
55	43642.0
56	38286.5
57	32931.0
58	30966.5
59	29002.0
60	26709.0
61	24416.0
62	21299.0
63	18182.0
64	15533.5
65	12885.0
66	10876.0
67	8867.0
68	7089.5
69	5312.0
70	4309.5
71	3307.0
72	2868.0
73	2429.0
74	2026.0
75	1178.5
76	734.0
77	581.5
78	429.0
79	326.0
80	223.0
81	120.5
82	18.0
83	22.0
84	26.0
85	13.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	723034.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.04711746494724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.02338943048261	18.668837217550607
2	13.140822425526387	7.108427354584503
3	5.4986096521263965	4.4616462346486605
4	2.713057152414245	2.9352150196185334
5	1.5916962637317709	2.152539790683542
6	0.9885721618931188	1.6042816431180014
7	0.7110413365359709	1.346213298620504
8	0.5021732388213064	1.08658708625223
9	0.39091720566156424	0.9515865222537541
>10	3.866677133359717	25.34968627093287
>50	1.4677454052109122	26.405132943601927
>100	0.0959387191927419	4.620443372496408
>500	0.0070199062823957485	1.3654690194042873
>1k	0.002339968760798583	1.9439342262341917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	4208	0.5819919948439493	RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	3887	0.5375957423855586	TruSeq Adapter, Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	3121	0.43165328324809066	Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1070	0.14798750819463538	Illumina PCR Primer Index 3 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	978	0.13526334861154524	No Hit
ACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	906	0.12530531067695294	TruSeq Adapter, Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	863	0.11935814913268256	TruSeq Adapter, Index 7 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCTT	764	0.10566584697261816	Illumina PCR Primer Index 3 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	761	0.10525092872534349	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5638738980462883	0.0
2	0.0	0.0	0.0	1.9170606084914403	0.0
3	0.0	0.0	0.0	2.660870719772514	0.0
4	0.0	0.0	0.0	3.4326186597034165	0.0
5	0.0	0.0	0.0	5.451610850942003	0.0
6	0.0	0.0	0.0	6.651969340307648	0.0
7	0.0	0.0	0.0	7.717341093226598	0.0
8	0.0	0.0	0.0	9.393749118298725	0.0
9	0.0	0.0	0.0	9.998976534990057	0.0
10	0.0	0.0	0.0	11.32422541678538	0.0
11	0.0	0.0	0.0	13.354558706782807	0.0
12	0.0	0.0	0.0	14.93871657487753	0.0
13	0.0	0.0	0.0	15.574924554032037	0.0
14	0.0	0.0	0.0	15.880719302273475	0.0
15	0.0	0.0	0.0	16.423985594038456	0.0
16	0.0	0.0	0.0	17.410522879975215	0.0
17	0.0	0.0	0.0	18.499406666906395	0.0
18	1.3830608242489288E-4	0.0	0.0	19.633931461037793	0.0
19	1.3830608242489288E-4	0.0	0.0	20.426287007250004	0.0
20	1.3830608242489288E-4	0.0	0.0	21.089188060312516	0.0
21	1.3830608242489288E-4	0.0	0.0	21.840190087879684	0.0
22	1.3830608242489288E-4	0.0	0.0	22.63268394017432	0.0
23	1.3830608242489288E-4	0.0	0.0	23.37690896970267	0.0
24	1.3830608242489288E-4	0.0	0.0	23.980338407322478	0.0
25	1.3830608242489288E-4	0.0	0.0	24.50396523538312	0.0
26	1.3830608242489288E-4	0.0	0.0	24.986100238716297	0.0
27	1.3830608242489288E-4	0.0	0.0	25.562559990263253	0.0
28	1.3830608242489288E-4	0.0	0.0	26.063228008641364	0.0
29	1.3830608242489288E-4	0.0	0.0	26.671498159146044	0.0
30	2.7661216484978576E-4	0.0	0.0	27.252936929660294	0.0
31	2.7661216484978576E-4	0.0	0.0	27.793298793694348	0.0
32	2.7661216484978576E-4	0.0	0.0	28.372939585137075	0.0
33	2.7661216484978576E-4	0.0	0.0	28.920769977622076	0.0
34	2.7661216484978576E-4	0.0	0.0	29.484643875668365	0.0
35	2.7661216484978576E-4	0.0	0.0	30.0821261517439	0.0
36	2.7661216484978576E-4	0.0	0.0	30.63839321525682	0.0
37	2.7661216484978576E-4	0.0	0.0	31.22688559597474	0.0
38	2.7661216484978576E-4	0.0	0.0	31.85161417028798	0.0
39	2.7661216484978576E-4	0.0	0.0	32.83192768251562	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGG	35	1.2110831E-7	45.000004	2
CTAACGC	55	1.8189894E-12	45.000004	29
TATCACG	40	6.8084773E-9	45.0	1
CACGGAA	20	7.0315483E-4	45.0	8
ACCGAAC	20	7.0315483E-4	45.0	11
GCCGGAC	20	7.0315483E-4	45.0	13
ATTCGTC	20	7.0315483E-4	45.0	19
CGTTCCA	20	7.0315483E-4	45.0	24
CGCTACA	20	7.0315483E-4	45.0	29
CGCTAAC	25	3.8893646E-5	45.0	37
TGCGAAG	40	6.8084773E-9	45.0	1
TCGTCTC	20	7.0315483E-4	45.0	31
ACTACGG	45	3.8380676E-10	45.0	2
TATTACG	20	7.0315483E-4	45.0	21
CTACCGA	25	3.8893646E-5	45.0	40
GTCCCCG	20	7.0315483E-4	45.0	29
GACGCGA	40	6.8084773E-9	45.0	26
GCTACGA	25	3.8893646E-5	45.0	10
ATCGGAA	25	3.8893646E-5	45.0	21
GCTAACG	20	7.0315483E-4	45.0	1
>>END_MODULE