SRR2935122.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935122.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	609006
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	2761	0.4533617074380219	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	2394	0.39309957537364165	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1937	0.31805926378393645	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	988	0.16223157078912195	No Hit
TCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	665	0.10919432649267823	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCC	658	0.10804491252959741	No Hit
ACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	611	0.10032741877748332	Illumina Single End Adapter 2 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTG	30	2.1638134E-6	45.000004	13
TGACGCG	30	2.1638134E-6	45.000004	25
AAGCCCG	30	2.1638134E-6	45.000004	1
CTACGTT	30	2.1638134E-6	45.000004	19
CTACGGT	30	2.1638134E-6	45.000004	12
CGTCGAA	30	2.1638134E-6	45.000004	31
CCCGGAT	30	2.1638134E-6	45.000004	37
TGCACGA	30	2.1638134E-6	45.000004	38
CACTTCG	30	2.1638134E-6	45.000004	30
TCCGGAT	20	7.030671E-4	45.0	14
AACCGTA	20	7.030671E-4	45.0	13
TAATACG	20	7.030671E-4	45.0	1
CGAACGT	20	7.030671E-4	45.0	27
TCCGCTA	20	7.030671E-4	45.0	13
GTCGATG	40	6.8066583E-9	45.0	1
GTCGACT	20	7.030671E-4	45.0	34
ACCATTC	20	7.030671E-4	45.0	29
TCCGATG	20	7.030671E-4	45.0	30
ACGTTAT	25	3.8886377E-5	45.0	44
CGTGCGG	35	1.2106648E-7	45.0	2