##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935122.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	609006
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.051600476842594	33.0	31.0	34.0	30.0	34.0
2	32.362713996249624	34.0	31.0	34.0	30.0	34.0
3	32.413734840050836	34.0	31.0	34.0	30.0	34.0
4	35.921192894651284	37.0	35.0	37.0	35.0	37.0
5	30.39549035641685	37.0	35.0	37.0	0.0	37.0
6	32.88245764409546	37.0	35.0	37.0	17.0	37.0
7	35.064411516471104	37.0	35.0	37.0	32.0	37.0
8	35.62249304604553	37.0	35.0	37.0	35.0	37.0
9	37.493817794898575	39.0	37.0	39.0	35.0	39.0
10	37.446018594233884	39.0	37.0	39.0	35.0	39.0
11	37.30397401667636	39.0	37.0	39.0	34.0	39.0
12	37.04746751263534	39.0	37.0	39.0	34.0	39.0
13	36.98797056186638	39.0	37.0	39.0	33.0	39.0
14	38.16377671155949	40.0	37.0	41.0	33.0	41.0
15	38.270553984689805	40.0	37.0	41.0	34.0	41.0
16	38.36846106606503	40.0	37.0	41.0	34.0	41.0
17	38.34909179876717	40.0	37.0	41.0	34.0	41.0
18	38.262823354778114	40.0	37.0	41.0	34.0	41.0
19	38.25319783384729	40.0	37.0	41.0	34.0	41.0
20	38.17221012600861	40.0	36.0	41.0	34.0	41.0
21	38.10755723260526	40.0	36.0	41.0	34.0	41.0
22	38.10254578772623	40.0	36.0	41.0	34.0	41.0
23	38.044060321244785	40.0	36.0	41.0	34.0	41.0
24	38.016334814435325	40.0	36.0	41.0	34.0	41.0
25	37.948905265301164	40.0	36.0	41.0	34.0	41.0
26	37.875687595852916	40.0	36.0	41.0	34.0	41.0
27	37.83273399605258	40.0	36.0	41.0	34.0	41.0
28	37.78508750324299	40.0	36.0	41.0	33.0	41.0
29	37.75962962598069	40.0	36.0	41.0	33.0	41.0
30	37.63401838405533	40.0	36.0	41.0	33.0	41.0
31	32.98924476934546	38.0	33.0	41.0	9.0	41.0
32	33.85889958391215	38.0	33.0	41.0	16.0	41.0
33	35.80295432228911	38.0	34.0	41.0	29.0	41.0
34	36.59383158786613	38.0	35.0	41.0	31.0	41.0
35	36.754701924118976	39.0	35.0	41.0	31.0	41.0
36	36.89290417499992	39.0	35.0	41.0	32.0	41.0
37	36.97070472212096	39.0	35.0	41.0	32.0	41.0
38	36.998164221698964	39.0	35.0	41.0	32.0	41.0
39	36.996786566963216	39.0	35.0	41.0	32.0	41.0
40	36.93574775946378	39.0	35.0	41.0	32.0	41.0
41	36.887372209797604	39.0	35.0	41.0	32.0	41.0
42	36.77048666187197	39.0	35.0	41.0	32.0	41.0
43	36.74301402613439	39.0	35.0	41.0	32.0	41.0
44	36.67879298397717	39.0	35.0	41.0	32.0	41.0
45	36.62398892621748	39.0	35.0	41.0	31.0	41.0
46	36.572385165334985	39.0	35.0	41.0	31.0	41.0
47	36.45055221130826	39.0	35.0	41.0	31.0	41.0
48	36.44981822839184	39.0	35.0	41.0	31.0	41.0
49	36.41319461548819	39.0	35.0	41.0	31.0	41.0
50	36.22795834523798	38.0	35.0	40.0	31.0	41.0
51	35.58099263389852	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	5.0
12	8.0
13	11.0
14	13.0
15	24.0
16	50.0
17	83.0
18	165.0
19	297.0
20	549.0
21	837.0
22	1398.0
23	2189.0
24	2929.0
25	3587.0
26	4090.0
27	4711.0
28	5773.0
29	7048.0
30	9036.0
31	12706.0
32	20847.0
33	22692.0
34	42752.0
35	65360.0
36	67252.0
37	68648.0
38	102526.0
39	163229.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5882503620654	23.303547091490067	23.181380807414047	10.926821739030485
2	32.348942374951974	24.37020324922907	24.488100281442218	18.792754094376736
3	31.241071516536785	24.142947688528523	26.238165141230134	18.37781565370456
4	28.213186733792444	26.881015950581766	25.103365155679914	19.80243215994588
5	22.069076495141264	39.73179246181483	20.66350742028814	17.535623622755768
6	25.0613294450301	33.75993011563104	24.2477085611636	16.931031878175258
7	77.76803512609071	7.99171108330624	8.375122740991058	5.865131049611991
8	78.48526943905314	6.187295363264073	8.716334486031336	6.611100711651445
9	73.22259550809022	8.126685122970875	11.194799394423043	7.455919974515851
10	41.807469877144065	26.1025342935866	17.6356226375438	14.454373191725534
11	30.76143748994263	25.828152760399732	23.761670656775138	19.6487390928825
12	26.87099962890349	23.70370735263692	28.840602555639844	20.584690462819744
13	24.17168303760554	27.279369989786634	28.425171508983492	20.123775463624334
14	18.898007572995997	30.764557327842418	28.074107644259662	22.263327454901923
15	17.233984558444416	27.852599153374513	34.29949787030013	20.613918417880942
16	21.586158428652592	27.2772353638552	29.93254582056663	21.20406038692558
17	21.176638653806368	27.073296486405717	26.247688856924235	25.50237600286368
18	20.916214290171197	27.243902358925858	30.317271094209254	21.522612256693694
19	23.521443138491247	28.559324538674495	26.71106688604053	21.208165436793728
20	24.750659271008825	30.213167029553073	25.52257284821496	19.51360085122314
21	23.376616979143066	29.354062193147524	27.796277869183555	19.47304295852586
22	21.295028292003693	27.35211147345018	26.821246424501567	24.531613810044565
23	21.117690137699793	27.602191111417625	27.06607159863778	24.214047152244806
24	22.37564161929439	26.56541971671872	28.343727319599477	22.715211344387413
25	20.55004384193259	29.799870608828154	25.292033247619894	24.35805230161936
26	19.63806596322532	31.07013724002719	25.756724892693995	23.53507190405349
27	21.500116583416258	29.880822192227992	26.316653694709085	22.30240752964667
28	18.5083562395116	29.012522044117794	28.00021674663304	24.478904969737574
29	19.91343270838054	28.819748902309666	26.770672210126005	24.496146179183782
30	23.558224385309835	29.062275248519718	25.35902766146803	22.020472704702417
31	25.656397473916513	31.149282601485044	22.179256033602297	21.015063890996146
32	25.044088235583885	31.42497775062971	21.328032892943583	22.202901120842817
33	25.811568358932423	28.794297593127162	23.27940939826537	22.114724649675043
34	22.085168290624395	29.63353398817089	24.868556303221972	23.412741417982748
35	22.796162927787247	28.577386758094335	25.136698160609257	23.489752153509162
36	24.97725802373047	30.854375818957447	24.193686104898802	19.974680052413277
37	21.392235872881386	30.928595120573526	26.294650627415823	21.38451837912927
38	22.698462740925375	29.873268900470602	23.168080445841255	24.260187912762767
39	23.486468113614645	28.204319826077246	25.207140816346634	23.102071243961472
40	24.956404370400293	26.443746038626877	27.80071132304115	20.79913826793168
41	21.70471226884464	27.612864241074803	24.447213984755486	26.23520950532507
42	23.196979996913004	26.934545800862388	25.95869334620677	23.90978085601784
43	23.751490133102138	26.11665566513302	26.46246506602562	23.669389135739223
44	22.093870996344865	26.014357822418827	25.5399782596559	26.351792921580415
45	21.74297133361576	26.27740941796961	25.42651468130035	26.553104567114282
46	24.396968174369384	26.824037858411902	25.980368009510578	22.798625957708136
47	21.21506192057221	26.6504763499867	28.680341408787434	23.454120320653654
48	21.882707231127444	24.309284309185788	29.25291376439641	24.555094695290357
49	22.527200060426335	23.07973977267876	30.755690420127223	23.637369746767682
50	21.132796721214568	24.217988000118226	29.450777168041036	25.19843811062617
51	20.646758816826107	24.521269084376836	27.22124248365369	27.610729615143363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1202.0
1	1214.5
2	1227.0
3	1332.0
4	1437.0
5	1262.0
6	1087.0
7	1125.5
8	1164.0
9	1282.5
10	1401.0
11	1484.5
12	1568.0
13	1642.0
14	1716.0
15	1780.0
16	1844.0
17	1839.5
18	1835.0
19	2038.0
20	2241.0
21	2272.5
22	2304.0
23	2588.0
24	2872.0
25	3027.5
26	3964.0
27	4745.0
28	5271.5
29	5798.0
30	7030.5
31	8263.0
32	9634.0
33	11005.0
34	12317.5
35	13630.0
36	15683.5
37	17737.0
38	17910.5
39	18084.0
40	21278.0
41	24472.0
42	29394.0
43	34316.0
44	39983.5
45	45651.0
46	51356.0
47	57061.0
48	63378.5
49	69696.0
50	67546.5
51	65397.0
52	56412.0
53	47427.0
54	41820.0
55	36213.0
56	32766.0
57	29319.0
58	27143.5
59	24968.0
60	21876.5
61	18785.0
62	18536.0
63	18287.0
64	15517.0
65	12747.0
66	10043.0
67	7339.0
68	5999.0
69	4659.0
70	3999.0
71	3339.0
72	2645.5
73	1952.0
74	1691.5
75	1173.5
76	916.0
77	664.0
78	412.0
79	270.5
80	129.0
81	104.0
82	79.0
83	72.0
84	65.0
85	33.0
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	609006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.767207882834263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.05870804269153	19.020432100643514
2	12.563342055968718	6.725711770305383
3	5.109495072417825	4.103007503391757
4	2.587657320726197	2.770574457336642
5	1.5547301731736614	2.0807892873527156
6	0.9621138309657548	1.5451860552246255
7	0.6119512280604186	1.146615801495426
8	0.4491093336100158	0.9617122315888367
9	0.3358498576806702	0.8090786662162921
>10	2.891426792951345	18.629896483299436
>50	1.616401905365397	30.94922701039302
>100	0.2514871282576324	8.97297053924174
>500	0.0056198240951426245	0.9512627401094712
>1k	0.002107434035678484	1.3335353534011405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	2761	0.4533617074380219	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	2394	0.39309957537364165	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1937	0.31805926378393645	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	988	0.16223157078912195	No Hit
TCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	665	0.10919432649267823	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCC	658	0.10804491252959741	No Hit
ACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	611	0.10032741877748332	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5329996748800504	0.0
2	0.0	0.0	0.0	1.6477670170737233	0.0
3	0.0	0.0	0.0	2.3099936618030035	0.0
4	0.0	0.0	0.0	3.114911839949032	0.0
5	0.0	0.0	0.0	4.936568769437411	0.0
6	0.0	0.0	0.0	6.154947570303084	0.0
7	0.0	0.0	0.0	7.086301284388003	0.0
8	0.0	0.0	0.0	8.747861269018696	0.0
9	0.0	0.0	0.0	9.35442343753592	0.0
10	0.0	0.0	0.0	10.560979694781333	0.0
11	0.0	0.0	0.0	12.43156881869801	0.0
12	0.0	0.0	0.0	13.872441322417185	0.0
13	0.0	0.0	0.0	14.481138116865843	0.0
14	0.0	0.0	0.0	14.75108619619511	0.0
15	0.0	0.0	0.0	15.22579416294749	0.0
16	0.0	0.0	0.0	16.146638949369958	0.0
17	0.0	0.0	0.0	17.191456241810425	0.0
18	4.926059841774958E-4	0.0	0.0	18.359917636279445	0.0
19	4.926059841774958E-4	0.0	0.0	19.131667011490855	0.0
20	4.926059841774958E-4	0.0	0.0	19.765322509137842	0.0
21	4.926059841774958E-4	0.0	0.0	20.430176385782733	0.0
22	4.926059841774958E-4	0.0	0.0	21.151187344623864	0.0
23	4.926059841774958E-4	0.0	0.0	21.902411470494542	0.0
24	4.926059841774958E-4	0.0	0.0	22.459877242588743	0.0
25	4.926059841774958E-4	0.0	0.0	22.96315635642342	0.0
26	6.568079789033277E-4	0.0	0.0	23.411099398035486	0.0
27	6.568079789033277E-4	0.0	0.0	23.82767985865492	0.0
28	6.568079789033277E-4	0.0	0.0	24.233751391611904	0.0
29	6.568079789033277E-4	0.0	0.0	24.73817991940966	0.0
30	6.568079789033277E-4	0.0	0.0	25.25656561675911	0.0
31	6.568079789033277E-4	0.0	0.0	25.72552651369609	0.0
32	6.568079789033277E-4	0.0	0.0	26.23291067739891	0.0
33	6.568079789033277E-4	0.0	0.0	26.72600926756058	0.0
34	9.852119683549916E-4	0.0	0.0	27.23355763325813	0.0
35	9.852119683549916E-4	0.0	0.0	27.787082557478907	0.0
36	9.852119683549916E-4	0.0	0.0	28.283465187535096	0.0
37	9.852119683549916E-4	0.0	0.0	28.79249137118518	0.0
38	0.0011494139630808236	0.0	0.0	29.299875534888	0.0
39	0.0011494139630808236	0.0	0.0	29.846339773335565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTG	30	2.1638134E-6	45.000004	13
TGACGCG	30	2.1638134E-6	45.000004	25
AAGCCCG	30	2.1638134E-6	45.000004	1
CTACGTT	30	2.1638134E-6	45.000004	19
CTACGGT	30	2.1638134E-6	45.000004	12
CGTCGAA	30	2.1638134E-6	45.000004	31
CCCGGAT	30	2.1638134E-6	45.000004	37
TGCACGA	30	2.1638134E-6	45.000004	38
CACTTCG	30	2.1638134E-6	45.000004	30
TCCGGAT	20	7.030671E-4	45.0	14
AACCGTA	20	7.030671E-4	45.0	13
TAATACG	20	7.030671E-4	45.0	1
CGAACGT	20	7.030671E-4	45.0	27
TCCGCTA	20	7.030671E-4	45.0	13
GTCGATG	40	6.8066583E-9	45.0	1
GTCGACT	20	7.030671E-4	45.0	34
ACCATTC	20	7.030671E-4	45.0	29
TCCGATG	20	7.030671E-4	45.0	30
ACGTTAT	25	3.8886377E-5	45.0	44
CGTGCGG	35	1.2106648E-7	45.0	2
>>END_MODULE