##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935121.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1493191
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.153964897993625	33.0	31.0	34.0	30.0	34.0
2	32.47758324286712	34.0	31.0	34.0	30.0	34.0
3	32.52963753464895	34.0	31.0	34.0	31.0	34.0
4	35.99580093906272	37.0	35.0	37.0	35.0	37.0
5	30.502752829343333	37.0	35.0	37.0	0.0	37.0
6	32.96794248023193	37.0	35.0	37.0	17.0	37.0
7	35.105979744051496	37.0	35.0	37.0	32.0	37.0
8	35.609750527561445	37.0	35.0	37.0	35.0	37.0
9	37.49996885863898	39.0	37.0	39.0	35.0	39.0
10	37.47199788908452	39.0	37.0	39.0	35.0	39.0
11	37.37644212964048	39.0	37.0	39.0	35.0	39.0
12	37.23719805436813	39.0	37.0	39.0	34.0	39.0
13	37.200593895891416	39.0	37.0	39.0	34.0	39.0
14	38.456497527777756	40.0	38.0	41.0	34.0	41.0
15	38.52788156371154	40.0	38.0	41.0	34.0	41.0
16	38.60490720878977	40.0	38.0	41.0	35.0	41.0
17	38.55202850807432	40.0	38.0	41.0	34.0	41.0
18	38.5329251247831	40.0	38.0	41.0	34.0	41.0
19	38.51673161705368	40.0	38.0	41.0	34.0	41.0
20	38.39284391615004	40.0	37.0	41.0	34.0	41.0
21	38.37230334230517	40.0	37.0	41.0	34.0	41.0
22	38.37577175324523	40.0	37.0	41.0	34.0	41.0
23	38.27839305219493	40.0	37.0	41.0	34.0	41.0
24	38.271389929352644	40.0	37.0	41.0	34.0	41.0
25	38.22699641238127	40.0	37.0	41.0	34.0	41.0
26	38.14197848768175	40.0	37.0	41.0	34.0	41.0
27	38.06352368853013	40.0	37.0	41.0	34.0	41.0
28	38.009957868752224	40.0	37.0	41.0	34.0	41.0
29	37.98624154579019	40.0	36.0	41.0	34.0	41.0
30	37.86939581071678	40.0	36.0	41.0	33.0	41.0
31	33.323808541573044	39.0	34.0	41.0	9.0	41.0
32	34.205768049767244	39.0	33.0	41.0	16.0	41.0
33	36.13373573775893	39.0	34.0	41.0	30.0	41.0
34	36.92960779967198	39.0	35.0	41.0	31.0	41.0
35	37.10353397522487	39.0	35.0	41.0	32.0	41.0
36	37.27828589912476	39.0	35.0	41.0	32.0	41.0
37	37.35276331025301	40.0	35.0	41.0	33.0	41.0
38	37.326851019059184	40.0	35.0	41.0	33.0	41.0
39	37.347028611878855	40.0	35.0	41.0	33.0	41.0
40	37.298626230669754	40.0	35.0	41.0	32.0	41.0
41	37.21469791875252	39.0	35.0	41.0	32.0	41.0
42	37.13569730864973	39.0	35.0	41.0	32.0	41.0
43	37.11505560909489	39.0	35.0	41.0	32.0	41.0
44	37.07133246851876	39.0	35.0	41.0	32.0	41.0
45	37.02156187654493	39.0	35.0	41.0	32.0	41.0
46	36.9620892437739	39.0	35.0	41.0	32.0	41.0
47	36.83253448487166	39.0	35.0	41.0	32.0	41.0
48	36.81663765720528	39.0	35.0	41.0	32.0	41.0
49	36.77175324523119	39.0	35.0	41.0	32.0	41.0
50	36.55124896948883	39.0	35.0	41.0	31.0	41.0
51	35.927580597525704	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	14.0
10	17.0
11	19.0
12	15.0
13	26.0
14	37.0
15	50.0
16	79.0
17	136.0
18	236.0
19	467.0
20	971.0
21	1695.0
22	2704.0
23	3929.0
24	5505.0
25	7140.0
26	8449.0
27	10170.0
28	12504.0
29	15662.0
30	20524.0
31	29243.0
32	45810.0
33	52143.0
34	94373.0
35	141603.0
36	167519.0
37	176903.0
38	251821.0
39	442838.0
40	589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.71236432579623	21.081964731906368	23.189531680809754	11.016139261487647
2	31.488336053458667	25.64152877964038	24.107297726814586	18.762837440086365
3	32.78535699719593	25.599873023611845	24.184983702687735	17.42978627650448
4	28.315600616398033	24.701863324919586	27.057690543272763	19.92484551540962
5	24.084996494085487	39.25063839790087	18.995895367705806	17.668469740307838
6	27.186943934165154	35.261396566145926	21.75073383110399	15.80092566858493
7	79.19395442378102	6.425299911397805	9.108412788451043	5.272332876370136
8	79.73186283603371	7.264174509490079	7.496093935738965	5.507868718737255
9	74.02308211072797	7.735781959575165	9.340734038713066	8.900401890983806
10	41.71093985966966	25.904656537576237	16.751440371660422	15.632963231093678
11	31.380379335262536	26.071279561690368	22.941070499353398	19.6072706036937
12	28.97686900068377	22.55846706817815	26.304404459978663	22.160259471159417
13	25.95327724316581	23.853478891849736	28.348483214806414	21.844760650178042
14	20.997916542491886	27.993538669868755	27.960522130122673	23.048022657516686
15	20.92873584156347	26.425353487932888	31.425986360753583	21.21992430975006
16	23.401694759746075	25.97156023576354	28.723719872407482	21.903025132082902
17	22.351125877399475	26.847536584402132	27.581535115065652	23.21980242313274
18	22.916492263883185	26.415106975597897	27.77039240124003	22.898008359278887
19	24.017824913222753	28.12835062627621	25.80868756910536	22.045136891395675
20	26.576305375534677	27.336422467052106	26.47752363897184	19.60974851844138
21	25.287320912060146	27.866964105730613	26.33936314912158	20.506351833087663
22	23.113118147644876	24.972290885760764	27.875335439337633	24.039255527256728
23	23.73601233867603	26.721497785614833	27.561243002402236	21.981246873306898
24	23.804322420909315	25.602417909028386	27.17776895253186	23.415490717530442
25	22.786033400951386	27.9684246690477	25.486826534582647	23.75871539541827
26	21.40777703589159	28.29517456239691	26.839031309457397	23.458017092254106
27	23.03248546234206	28.728206907220844	25.9977457672863	22.241561863150796
28	20.196344606952493	29.19365305577116	27.481547906463405	23.128454430812937
29	21.858891461306694	28.00398609421032	26.4390824750484	23.698039969434586
30	22.436714392197647	29.137531635269703	26.2852508486858	22.140503123846848
31	24.757114126725916	29.18360745544274	22.93055610434298	23.12872231348836
32	23.933107017119713	29.256873367171384	24.133483258337346	22.676536357371564
33	24.84169808149125	27.76972269455147	23.985946874847222	23.40263234911006
34	21.82681251092459	29.278036098529924	25.807281185059377	23.0878702054861
35	21.886684288881998	27.982086685494355	25.184052140683942	24.947176884939704
36	22.521030464287556	31.264854931485658	24.02492380412151	22.189190800105276
37	21.833576548479066	30.876625964126493	25.465128037873253	21.824669449521195
38	22.56911540452628	28.833417827993873	24.266286094679113	24.331180672800734
39	23.967931764924916	26.484019793850887	25.516226658210506	24.031821783013694
40	23.603678297016256	25.46773989395864	27.38048916715946	23.548092641865644
41	20.737333669972564	27.927036795694587	25.95542030456921	25.38020922976364
42	21.83592052188903	27.35008448349876	25.895079731929805	24.918915262682404
43	23.048558422867536	27.019986056706745	25.775068293339565	24.156387227086153
44	22.488951513905455	26.663702098391965	25.755311946027	25.09203444167558
45	22.139632505151717	26.324696572642083	25.433517882173145	26.102153040033055
46	23.082445581308754	27.88357283160694	24.845314497609483	24.188667089474823
47	20.734855755224885	27.753850646032557	28.330200222208678	23.18109337653388
48	21.512720073989193	26.171400711630326	27.571556485406084	24.744322728974392
49	22.077416753784345	25.154451105049517	28.18869119891561	24.57944094225052
50	21.666417759014085	25.601279407657827	27.636116210183424	25.09618662314466
51	20.961953293316128	25.348197250050397	25.868961171075906	27.820888285557576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	870.0
1	1422.0
2	1974.0
3	2443.0
4	2912.0
5	2425.0
6	1938.0
7	1842.5
8	1747.0
9	1794.0
10	1841.0
11	1979.5
12	2118.0
13	2176.0
14	2234.0
15	2287.0
16	2340.0
17	2616.0
18	2892.0
19	2899.5
20	2907.0
21	3733.5
22	4560.0
23	4718.5
24	4877.0
25	6482.5
26	11202.0
27	14316.0
28	15941.0
29	17566.0
30	20222.5
31	22879.0
32	27018.5
33	31158.0
34	35542.5
35	39927.0
36	43648.5
37	47370.0
38	51091.5
39	54813.0
40	59803.5
41	64794.0
42	71374.0
43	77954.0
44	87640.5
45	97327.0
46	114434.0
47	131541.0
48	134585.0
49	137629.0
50	136635.5
51	135642.0
52	122050.0
53	108458.0
54	98759.0
55	89060.0
56	82961.5
57	76863.0
58	73998.0
59	71133.0
60	69790.5
61	68448.0
62	62696.5
63	56945.0
64	47921.0
65	38897.0
66	31553.5
67	24210.0
68	20024.0
69	15838.0
70	13340.0
71	10842.0
72	8931.5
73	7021.0
74	5942.5
75	3891.0
76	2918.0
77	2396.5
78	1875.0
79	1373.0
80	871.0
81	648.0
82	425.0
83	306.0
84	187.0
85	133.0
86	79.0
87	44.5
88	10.0
89	21.0
90	32.0
91	16.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1493191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.69679428233706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39872658868033	17.633196625814673
2	12.107459990706026	5.980308973441866
3	4.480840268734547	3.3198717098684702
4	2.2018129123989008	2.1751088218283634
5	1.184271343750703	1.462385287553899
6	0.7651659254517117	1.1338287271640985
7	0.5372854270800792	0.9288459364446028
8	0.38647474454535596	0.7635749809084338
9	0.29807414535368104	0.6625328263814966
>10	4.845045898264779	32.58699919809918
>50	1.706890766797228	26.91636663233391
>100	0.0796196946144257	3.3418824898297514
>500	0.004937655479873614	0.8097861123435077
>1k	0.002777431207428908	1.466227315695473
>5k	6.172069349842017E-4	0.8190843622922991
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC	5490	0.3676689720203242	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG	5257	0.3520648061768387	No Hit
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	3631	0.24317049861672085	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC	3036	0.203322950647305	No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT	3004	0.20117988924390784	No Hit
CCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC	2286	0.1530949490051842	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACGACGT	1970	0.1319322176466373	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1631	0.10922916090439869	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1514	0.10139359264822786	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.697066885616107E-5	0.0	0.0	0.2141721990020031	0.0
2	6.697066885616107E-5	0.0	0.0	0.7648720090062155	0.0
3	6.697066885616107E-5	0.0	0.0	1.0852596888140902	0.0
4	6.697066885616107E-5	0.0	0.0	1.476971130953776	0.0
5	6.697066885616107E-5	0.0	0.0	2.623241099095829	0.0
6	6.697066885616107E-5	0.0	0.0	3.3070116281172335	0.0
7	1.3394133771232213E-4	0.0	0.0	3.9035863462879163	0.0
8	1.3394133771232213E-4	0.0	0.0	4.823763336371569	0.0
9	1.3394133771232213E-4	0.0	0.0	5.1426106907957525	0.0
10	2.009120065684832E-4	0.0	0.0	6.252783468424334	0.0
11	2.009120065684832E-4	0.0	0.0	7.363692923410334	0.0
12	2.009120065684832E-4	0.0	0.0	8.668616406072632	0.0
13	2.009120065684832E-4	0.0	0.0	9.072985304626133	0.0
14	2.009120065684832E-4	0.0	0.0	9.266262654945013	0.0
15	2.6788267542464427E-4	0.0	0.0	9.665541782665446	0.0
16	2.6788267542464427E-4	0.0	0.0	10.309330822379723	0.0
17	2.6788267542464427E-4	0.0	0.0	11.039311112911879	0.0
18	3.3485334428080534E-4	0.0	0.0	11.748329584092055	0.0
19	3.3485334428080534E-4	0.0	0.0	12.425135163552419	0.0
20	3.3485334428080534E-4	0.0	0.0	12.873838644888698	0.0
21	3.3485334428080534E-4	0.0	0.0	13.396343803304466	0.0
22	3.3485334428080534E-4	0.0	0.0	13.975171294228266	0.0
23	3.3485334428080534E-4	0.0	0.0	14.514419119858076	0.0
24	3.3485334428080534E-4	0.0	0.0	14.965600515942032	0.0
25	4.018240131369664E-4	0.0	0.0	15.375862833354876	0.0
26	4.018240131369664E-4	0.0	0.0	15.747750957513137	0.0
27	4.018240131369664E-4	0.0	0.0	16.117763902943427	0.0
28	4.018240131369664E-4	0.0	0.0	16.50391677956805	0.0
29	4.018240131369664E-4	0.0	0.0	16.92790808409641	0.0
30	5.357653508492885E-4	0.0	0.0	17.36783840781253	0.0
31	5.357653508492885E-4	0.0	0.0	17.772676101048024	0.0
32	6.027360197054496E-4	0.0	0.0	18.23939469230661	0.0
33	6.027360197054496E-4	0.0	0.0	18.680731400068712	0.0
34	6.027360197054496E-4	0.0	0.0	19.12528269993591	0.0
35	6.027360197054496E-4	0.0	0.0	19.567222143717714	0.0
36	6.027360197054496E-4	0.0	0.0	20.02751155076611	0.0
37	6.027360197054496E-4	0.0	0.0	20.51184342793387	0.0
38	6.027360197054496E-4	0.0	0.0	20.96148449863413	0.0
39	6.027360197054496E-4	0.0	0.0	21.432020418017522	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATCG	30	2.1659398E-6	45.000004	1
TAACCGT	20	7.0339645E-4	45.000004	27
TCCGTTA	20	7.0339645E-4	45.000004	45
ATACGAG	20	7.0339645E-4	45.000004	1
GTCGAAA	25	3.8913684E-5	45.0	25
GCCGATT	45	3.8562575E-10	45.0	9
TCTAGCG	60	3.6379788E-12	41.250004	1
TACGGCT	880	0.0	40.909092	7
TTACGCG	55	6.184564E-11	40.909092	1
TACGTAG	50	1.0822987E-9	40.5	1
GTACGAG	95	0.0	40.263157	1
TAATACG	260	0.0	39.80769	4
CCGTCGA	40	3.459154E-7	39.375004	41
CGATGAA	705	0.0	38.936172	19
TTACGGG	395	0.0	38.734177	3
CGTAAGG	250	0.0	38.7	2
AGATCGT	35	6.2495437E-6	38.57143	13
GGCGATA	340	0.0	38.38235	8
TACGGGA	540	0.0	37.916664	4
CTACGGG	395	0.0	37.594936	3
>>END_MODULE