##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935120.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474068
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.835544689791337	33.0	31.0	34.0	30.0	34.0
2	32.15965009239181	34.0	31.0	34.0	30.0	34.0
3	32.2154374477923	34.0	31.0	34.0	30.0	34.0
4	35.758566281630486	37.0	35.0	37.0	35.0	37.0
5	30.27429187373963	37.0	35.0	37.0	0.0	37.0
6	32.751672755807185	37.0	35.0	37.0	17.0	37.0
7	34.923055342271574	37.0	35.0	37.0	32.0	37.0
8	35.46572010766388	37.0	35.0	37.0	33.0	37.0
9	37.26995705257474	39.0	37.0	39.0	34.0	39.0
10	37.28970738375085	39.0	37.0	39.0	34.0	39.0
11	37.19494460710278	39.0	37.0	39.0	34.0	39.0
12	36.94676923985588	39.0	35.0	39.0	33.0	39.0
13	36.81720976737515	39.0	35.0	39.0	33.0	39.0
14	37.896917741758564	40.0	37.0	41.0	33.0	41.0
15	38.05297763190091	40.0	37.0	41.0	33.0	41.0
16	38.14811377270771	40.0	37.0	41.0	34.0	41.0
17	38.11947020258697	40.0	36.0	41.0	34.0	41.0
18	38.03286659297822	40.0	36.0	41.0	34.0	41.0
19	37.99662284735523	40.0	36.0	41.0	34.0	41.0
20	37.919228886995114	40.0	36.0	41.0	34.0	41.0
21	37.85951593442291	40.0	36.0	41.0	33.0	41.0
22	37.83436342465638	39.0	35.0	41.0	33.0	41.0
23	37.753689344144725	39.0	35.0	41.0	33.0	41.0
24	37.76056599475181	39.0	35.0	41.0	33.0	41.0
25	37.69005290380283	39.0	35.0	41.0	33.0	41.0
26	37.62254360133989	39.0	35.0	41.0	33.0	41.0
27	37.58852949365914	39.0	35.0	41.0	33.0	41.0
28	37.50937207320469	39.0	35.0	41.0	33.0	41.0
29	37.461319895036155	39.0	35.0	41.0	33.0	41.0
30	37.310155926997815	39.0	35.0	41.0	33.0	41.0
31	32.752157918273326	38.0	33.0	40.0	9.0	41.0
32	33.47851574035792	38.0	32.0	40.0	16.0	41.0
33	35.37633208737987	38.0	33.0	40.0	27.0	41.0
34	36.27732519385405	38.0	35.0	40.0	30.0	41.0
35	36.39322417880979	38.0	35.0	40.0	31.0	41.0
36	36.56353729844663	39.0	35.0	40.0	31.0	41.0
37	36.59777711214425	39.0	35.0	40.0	31.0	41.0
38	36.479091607111215	39.0	35.0	40.0	31.0	41.0
39	36.489022671852986	39.0	35.0	40.0	31.0	41.0
40	36.52117417754415	39.0	35.0	40.0	31.0	41.0
41	36.470057038230806	39.0	35.0	41.0	31.0	41.0
42	36.35602909287275	39.0	35.0	41.0	31.0	41.0
43	36.289776994017735	39.0	35.0	40.0	31.0	41.0
44	36.194877106237925	39.0	35.0	40.0	31.0	41.0
45	36.16028713180388	39.0	35.0	40.0	30.0	41.0
46	36.113627158972974	39.0	35.0	40.0	30.0	41.0
47	36.00627125222542	38.0	35.0	40.0	30.0	41.0
48	36.010631386214634	38.0	35.0	40.0	30.0	41.0
49	35.965513386265265	38.0	35.0	40.0	30.0	41.0
50	35.75943113646144	38.0	35.0	40.0	29.0	41.0
51	35.12727920888987	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	3.0
12	6.0
13	7.0
14	10.0
15	23.0
16	28.0
17	102.0
18	165.0
19	315.0
20	491.0
21	797.0
22	1345.0
23	1996.0
24	2849.0
25	3315.0
26	3722.0
27	4266.0
28	5319.0
29	6431.0
30	8138.0
31	11415.0
32	17839.0
33	19812.0
34	36278.0
35	52861.0
36	53302.0
37	53660.0
38	82323.0
39	107152.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.70161242690922	22.900301222609414	23.038467055359146	12.359619295122219
2	31.531974315920923	25.19132276382291	24.413586236573657	18.86311668368251
3	31.331370183180475	25.9150585991883	25.94226988533291	16.81130133229832
4	27.40450737025068	26.621708278137312	26.321751309938655	19.65203304167335
5	22.129947602453655	39.016132706700304	21.421188521477934	17.432731169368108
6	25.191744644228255	33.828691242606546	24.297569125104417	16.681994988060783
7	74.18387235586455	8.354075786596017	10.816591712581317	6.645460144958107
8	72.52187449901702	8.10896327109191	10.92670249837576	8.44245973151531
9	66.86952082823561	9.831079085700784	13.55185332062067	9.747546765442932
10	38.102550688930705	24.78821603651797	21.06048077490993	16.048752499641402
11	29.509901533113393	25.633453428622055	24.337225883206628	20.519419155057925
12	26.399166364319044	23.62192765594809	30.607634347815083	19.371271631917782
13	22.509850907464752	27.568196967523644	30.55996186201136	19.361990263000244
14	16.365162803648424	32.75795877384679	29.15615481323354	21.720723609271246
15	16.22530944927732	27.72598023912181	36.27496477298615	19.773745538614715
16	18.038551431440215	28.21367398769797	32.15656825603078	21.591206324831035
17	17.25174447547609	27.865622653290245	27.490992853345936	27.39164001788773
18	18.656184344861916	29.137381135195795	30.14187838031675	22.064556139625537
19	20.18507893382384	29.88368757224702	27.694339208721114	22.236894285208027
20	23.39158095463098	29.54090974290608	28.030788831981912	19.03672047048103
21	21.376891078916948	29.011238893998332	29.28799243990314	20.323877587181585
22	18.513377827653414	28.018765240429644	27.417796603018974	26.050060328897963
23	18.816498898892142	27.99513993773045	28.3923403393606	24.796020824016807
24	21.878506880869413	26.57593425415763	27.64413544048533	23.901423424487625
25	19.16391741269185	29.57951179999494	25.283714572592963	25.972856214720252
26	18.05310630542454	30.34121687184117	26.285258654876515	25.320418167857778
27	23.412885915100787	27.80212965228617	26.0652480234903	22.719736409122742
28	19.27086409544622	27.569040728334333	28.22780698127695	24.932288194942497
29	20.085726098365637	24.78737227570728	29.39683758448155	25.730064041445534
30	23.299821966468944	25.657078731321242	28.474607018402427	22.568492283807387
31	27.599416117519006	27.334264282761122	24.142528076140977	20.923791523578895
32	24.8527637385354	26.333985841693597	27.288912139186785	21.52433828058422
33	24.073761570070115	25.068555565868188	29.957727583384663	20.899955280677034
34	21.41781347823519	23.002818161107687	32.322156315127785	23.257212045529332
35	23.230844520195415	22.812550098298136	29.482268366563446	24.474337014943004
36	24.971523072639368	22.803268729380598	31.233282988938292	20.99192520904174
37	24.507032746357062	24.044229941696127	32.323421956343815	19.125315355602993
38	22.676493667575116	25.585991883021002	29.58330872364302	22.15420572576086
39	23.12959322291317	22.09303306698617	33.872144924356846	20.90522878574382
40	23.69554578668039	22.336879941274248	32.71429415189382	21.253280120151537
41	21.563995038686436	23.2331648624248	29.276812609161556	25.92602748972721
42	24.826607153404154	21.72789557616207	29.470244775011178	23.9752524954226
43	25.559835297889755	21.307280812035405	29.54407384594615	23.588810044128692
44	21.971109629842132	22.112439565631934	29.737295071593106	26.17915573293283
45	21.777677463992507	21.52265075896285	30.117831197212215	26.581840579832427
46	23.90374376671701	22.262418049731263	31.896478986137012	21.937359197414715
47	20.5004345368175	21.520752297138806	34.31511934996667	23.663693816077018
48	20.98243289992153	19.620181071069972	33.367786899769655	26.029599129238846
49	22.015407072403114	18.524557658395	35.710277850434956	23.74975741876693
50	20.190352438890624	19.548250461959043	34.445691335420236	25.815705763730097
51	20.48461402161715	20.262704928406894	29.79445986651704	29.458221183458917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1483.0
1	1516.0
2	1549.0
3	1526.0
4	1503.0
5	1336.0
6	1169.0
7	1189.5
8	1210.0
9	1345.0
10	1480.0
11	1668.0
12	1856.0
13	2131.5
14	2407.0
15	2311.5
16	2216.0
17	2113.0
18	2010.0
19	1907.5
20	1805.0
21	1821.5
22	1838.0
23	1896.5
24	1955.0
25	2329.0
26	3292.0
27	3881.0
28	4226.0
29	4571.0
30	5686.0
31	6801.0
32	7560.0
33	8319.0
34	8511.0
35	8703.0
36	9830.0
37	10957.0
38	11583.5
39	12210.0
40	15220.0
41	18230.0
42	22664.0
43	27098.0
44	30667.5
45	34237.0
46	41836.0
47	49435.0
48	54942.0
49	60449.0
50	59822.0
51	59195.0
52	48484.5
53	37774.0
54	32101.5
55	26429.0
56	22800.5
57	19172.0
58	17419.5
59	15667.0
60	14917.0
61	14167.0
62	12411.0
63	10655.0
64	8895.0
65	7135.0
66	5659.5
67	4184.0
68	3558.5
69	2933.0
70	2646.5
71	2360.0
72	2203.0
73	2046.0
74	1599.0
75	851.5
76	551.0
77	415.5
78	280.0
79	249.0
80	218.0
81	141.0
82	64.0
83	37.0
84	10.0
85	5.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	474068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.422961170059352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96197325751304	21.588733569641093
2	11.94143767171622	7.265877892026132
3	5.15614252668711	4.705953716300812
4	2.796728332547239	3.40339029857158
5	1.7505133153203332	2.6627899309831182
6	1.1730803902351492	2.141314749688921
7	0.7642052965131368	1.6274571643241
8	0.5558160816734009	1.3527656856355532
9	0.42564036357466434	1.1654316228097765
>10	3.3927842528109515	24.626877310091512
>50	0.9530712998612323	19.467807539843246
>100	0.11712442480222372	5.739429472214676
>500	0.008420710279898437	1.7147954554421627
>1k	0.003062076465417614	2.537375592427321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT	3554	0.7496814802939663	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	3234	0.6821806154391353	No Hit
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2916	0.6151016309896471	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1191	0.2512297813815739	No Hit
TCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	996	0.21009644186066131	No Hit
ACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	917	0.19343216584962494	No Hit
AAAACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	825	0.17402566720386103	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	703	0.14829096247795673	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	655	0.13816583274973213	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCC	613	0.12930634423753554	No Hit
GGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	559	0.11791557329328282	No Hit
ACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	538	0.11348582903718454	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	537	0.11327488883451318	No Hit
AGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	514	0.10842326417307223	No Hit
TGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCTT	506	0.10673574255170146	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1094020267134673E-4	0.0	0.0	0.786596015761452	0.0
2	2.1094020267134673E-4	0.0	0.0	2.574103293198444	0.0
3	2.1094020267134673E-4	0.0	0.0	3.5659441261591165	0.0
4	2.1094020267134673E-4	0.0	0.0	4.639207877350929	0.0
5	2.1094020267134673E-4	0.0	0.0	7.476142663077871	0.0
6	2.1094020267134673E-4	0.0	0.0	9.217243095927167	0.0
7	2.1094020267134673E-4	0.0	0.0	10.639191002134714	0.0
8	2.1094020267134673E-4	0.0	0.0	12.86735236295215	0.0
9	2.1094020267134673E-4	0.0	0.0	13.646776411822776	0.0
10	2.1094020267134673E-4	0.0	0.0	15.37332197068775	0.0
11	2.1094020267134673E-4	0.0	0.0	18.026105959482607	0.0
12	2.1094020267134673E-4	0.0	0.0	19.92541154433541	0.0
13	2.1094020267134673E-4	0.0	0.0	20.792164837111976	0.0
14	2.1094020267134673E-4	0.0	0.0	21.13895053030367	0.0
15	2.1094020267134673E-4	0.0	0.0	21.798771484259643	0.0
16	2.1094020267134673E-4	0.0	0.0	22.972231831720343	0.0
17	2.1094020267134673E-4	0.0	0.0	24.376249820700828	0.0
18	2.1094020267134673E-4	0.0	0.0	26.023481863361376	0.0
19	2.1094020267134673E-4	0.0	0.0	26.9817832040973	0.0
20	2.1094020267134673E-4	0.0	0.0	27.82216897153995	0.0
21	2.1094020267134673E-4	0.0	0.0	28.653062429862384	0.0
22	2.1094020267134673E-4	0.0	0.0	29.562214703375886	0.0
23	2.1094020267134673E-4	0.0	0.0	30.363787473527005	0.0
24	2.1094020267134673E-4	0.0	0.0	31.045124328155456	0.0
25	2.1094020267134673E-4	0.0	0.0	31.60981125070665	0.0
26	2.1094020267134673E-4	0.0	0.0	32.16099800028688	0.0
27	2.1094020267134673E-4	0.0	0.0	32.641097901566866	0.0
28	2.1094020267134673E-4	0.0	0.0	33.170979690677285	0.0
29	2.1094020267134673E-4	0.0	0.0	33.72828370613499	0.0
30	4.2188040534269346E-4	0.0	0.0	34.35709645029827	0.0
31	4.2188040534269346E-4	0.0	0.0	34.907861319473156	0.0
32	4.2188040534269346E-4	0.0	0.0	35.48478277377929	0.0
33	4.2188040534269346E-4	0.0	0.0	36.02711003484732	0.0
34	6.328206080140401E-4	0.0	0.0	36.597914223275986	0.0
35	6.328206080140401E-4	0.0	0.0	37.1963515782546	0.0
36	6.328206080140401E-4	0.0	0.0	37.77833559742484	0.0
37	8.437608106853869E-4	0.0	0.0	38.37002286591797	0.0
38	8.437608106853869E-4	0.0	0.0	38.97162432393665	0.0
39	8.437608106853869E-4	0.0	0.0	39.59199945999308	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTCG	35	1.2099008E-7	45.000004	31
ACGCCGT	35	1.2099008E-7	45.000004	25
GGTCGAG	30	2.162793E-6	45.000004	1
TCTTACG	30	2.162793E-6	45.000004	28
GCTCTAG	30	2.162793E-6	45.000004	1
TACGCCG	30	2.162793E-6	45.000004	31
TACGCAT	35	1.2099008E-7	45.000004	28
GTTGTAA	30	2.162793E-6	45.000004	29
CTAGTAG	35	1.2099008E-7	45.000004	1
TTAAGCG	35	1.2099008E-7	45.000004	1
TTGCGAC	30	2.162793E-6	45.000004	27
TTTGCGA	30	2.162793E-6	45.000004	26
CGATGCA	30	2.162793E-6	45.000004	10
CCGGTAT	35	1.2099008E-7	45.000004	26
TAGGATC	30	2.162793E-6	45.000004	34
TTGCACG	35	1.2099008E-7	45.000004	1
ATACGCA	35	1.2099008E-7	45.000004	27
TATACGC	35	1.2099008E-7	45.000004	26
GACCTGC	35	1.2099008E-7	45.000004	16
CCGTTGT	35	1.2099008E-7	45.000004	38
>>END_MODULE