FastQCFastQC Report
Sat 14 Jan 2017
SRR2935119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791286
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG52270.660570261574197No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT38860.4910992991156169TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC33650.42525711310449066TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC29300.3702833109646828TruSeq Adapter, Index 20 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC26810.3388155483605169No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10310.13029422989917677No Hit
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC10180.12865133466281473TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC10050.12700843942645262No Hit
ACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC9510.12018410536771787TruSeq Adapter, Index 27 (95% over 22bp)
GGGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTC9410.11892033980128551No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG9230.11664556178170725No Hit
TGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCTT8510.10754644970339422TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT253.8896993E-545.017
TCACCCG207.0319534E-445.037
CGACGTC253.8896993E-545.018
CCTCGAG253.8896993E-545.033
CTACGTA207.0319534E-445.012
CGCTAAT253.8896993E-545.025
AATTCGC207.0319534E-445.018
ACGCTAA253.8896993E-545.018
TGCTCGA253.8896993E-545.02
TTAGTCG207.0319534E-445.029
ACGTACC207.0319534E-445.014
GCTACGT207.0319534E-445.011
GGATTCG207.0319534E-445.09
GACGACT406.8102963E-945.027
CGCCGCA207.0319534E-445.018
GCTAACG207.0319534E-445.01
TATCGCG207.0319534E-445.01
CTAGGCG207.0319534E-445.01
CCCGATT207.0319534E-445.013
TAATCCG207.0319534E-445.028
Produced by FastQC (version 0.11.5)