##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	791286
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865627598617944	33.0	31.0	34.0	30.0	34.0
2	32.19688835642233	34.0	31.0	34.0	30.0	34.0
3	32.26605550963874	34.0	31.0	34.0	30.0	34.0
4	35.80108582737468	37.0	35.0	37.0	35.0	37.0
5	30.290700201949736	37.0	35.0	37.0	0.0	37.0
6	32.73761067426948	37.0	35.0	37.0	17.0	37.0
7	34.96636614321497	37.0	35.0	37.0	32.0	37.0
8	35.57587774837417	37.0	35.0	37.0	35.0	37.0
9	37.44713289505943	39.0	37.0	39.0	35.0	39.0
10	37.3477124579482	39.0	37.0	39.0	34.0	39.0
11	37.12075406363818	39.0	37.0	39.0	34.0	39.0
12	36.6781972636948	39.0	35.0	39.0	33.0	39.0
13	36.40794857990663	39.0	35.0	39.0	33.0	39.0
14	36.75161319674555	39.0	35.0	41.0	27.0	41.0
15	37.118999957031974	40.0	35.0	41.0	31.0	41.0
16	37.50704928432956	40.0	35.0	41.0	33.0	41.0
17	37.65917632815442	40.0	35.0	41.0	33.0	41.0
18	37.67015845092672	39.0	35.0	41.0	33.0	41.0
19	37.64718698422568	39.0	35.0	41.0	33.0	41.0
20	37.48066185930245	39.0	35.0	41.0	33.0	41.0
21	37.470679375093205	39.0	35.0	41.0	33.0	41.0
22	37.565565421352076	39.0	35.0	41.0	33.0	41.0
23	37.5465369032183	39.0	35.0	41.0	33.0	41.0
24	37.5043220782372	39.0	35.0	41.0	33.0	41.0
25	37.40596320420177	39.0	35.0	41.0	33.0	41.0
26	37.33760486094788	39.0	35.0	41.0	33.0	41.0
27	37.315739189117465	39.0	35.0	41.0	33.0	41.0
28	37.279547470825975	39.0	35.0	41.0	33.0	41.0
29	37.24175456156181	39.0	35.0	41.0	33.0	41.0
30	37.092585992927965	39.0	35.0	41.0	32.0	41.0
31	32.493863154409404	37.0	32.0	40.0	9.0	41.0
32	33.36083034452777	37.0	32.0	40.0	16.0	41.0
33	35.259525632956986	37.0	33.0	40.0	27.0	41.0
34	36.02389275179897	38.0	34.0	40.0	30.0	41.0
35	36.23921186524215	38.0	35.0	40.0	30.0	41.0
36	36.3877889915909	38.0	35.0	40.0	31.0	41.0
37	36.465860636988396	39.0	35.0	40.0	31.0	41.0
38	36.441027643608	39.0	35.0	40.0	31.0	41.0
39	36.42723111491926	38.0	35.0	40.0	31.0	41.0
40	36.33270524184682	38.0	35.0	40.0	31.0	41.0
41	36.1918067550797	38.0	35.0	40.0	30.0	41.0
42	36.22931026202915	38.0	35.0	40.0	30.0	41.0
43	36.25415715683078	38.0	35.0	40.0	31.0	41.0
44	36.22988527536188	38.0	35.0	40.0	31.0	41.0
45	36.26275708151035	38.0	35.0	40.0	31.0	41.0
46	36.28290529593598	38.0	35.0	40.0	31.0	41.0
47	36.15178582712193	38.0	35.0	40.0	31.0	41.0
48	36.096641416630646	38.0	35.0	40.0	31.0	41.0
49	36.08455729028442	38.0	35.0	40.0	31.0	41.0
50	35.875934870577765	38.0	35.0	40.0	30.0	41.0
51	35.20858071544296	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	5.0
12	9.0
13	13.0
14	22.0
15	34.0
16	80.0
17	145.0
18	283.0
19	467.0
20	794.0
21	1290.0
22	2031.0
23	3049.0
24	4067.0
25	5321.0
26	6360.0
27	7575.0
28	9419.0
29	11955.0
30	16368.0
31	24591.0
32	33060.0
33	41155.0
34	78996.0
35	73340.0
36	82766.0
37	82203.0
38	125952.0
39	179802.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53883677962204	22.42880576681503	25.021168073237742	11.011189380325192
2	34.057344626342434	24.16117560528052	25.183941078194234	16.597538690182816
3	33.93804515687122	23.063847963947296	25.70132670109164	17.296780178089843
4	30.48088807333884	25.417105825201002	25.22539258877321	18.876613512686944
5	22.865563146574058	37.95176459586041	22.62860710286799	16.554065154697543
6	27.79121581830084	30.72087715440435	26.696036578430554	14.791870448864252
7	77.88157505630076	6.5787844091769605	9.82249148853891	5.717149045983374
8	78.25110010792558	5.493462540724845	9.83651928632631	6.418918065023266
9	72.964768743539	7.175660886202966	12.336753082956099	7.522817287301936
10	47.1486921290153	19.324997535657147	19.54426086143316	13.982049473894396
11	36.217246355931984	23.858630128676612	22.51385718943593	17.41026632595547
12	33.050123469895844	20.374428462022582	29.129922682822645	17.445525385258932
13	30.548625907699616	21.516746157520792	31.43819048991136	16.496437444868228
14	20.483365053849052	24.80670705661417	36.675108620650434	18.034819268886345
15	18.94953278587009	26.032306902940277	37.66754371996977	17.35061659121986
16	28.744095055390844	25.28390493449903	27.182586321507014	18.78941368860311
17	27.29708348182579	24.11125686540644	25.230194897925657	23.361464754842117
18	27.544402403176598	23.535864403009786	29.584120027398438	19.33561316641518
19	26.163485768735956	26.142886390003106	26.75568631316616	20.937941528094772
20	21.61329784679623	33.86613689614122	26.15261738486464	18.367947872197917
21	21.957168457422473	26.019416494162666	34.69921115753343	17.324203890881424
22	19.260924621439024	23.31167239152468	34.92997474996398	22.49742823707231
23	19.814706692649686	24.602608917635344	33.846927659531445	21.735756730183525
24	22.964768743539	29.115389378808676	26.066175820120662	21.853666057531665
25	24.805696044161024	26.170057349681404	25.59163184992531	23.432614756232258
26	18.110518826315644	26.486883377186	26.465778492226576	28.936819304271783
27	20.49739285163645	24.493166819582303	32.10975551191352	22.899684816867733
28	18.827200279039438	25.72799215454336	26.374408241773516	29.07039932464368
29	21.900551760046305	27.515714924818585	26.24841081480021	24.335322500334897
30	25.35063175640666	23.051589437952902	29.69229835988505	21.90548044575539
31	24.973397734826598	24.484067707503986	28.59825650902455	21.944278048644865
32	27.973324436423745	23.472802501244807	26.09410503913882	22.459768023192623
33	27.343084548443926	21.3428520155797	29.20347383878901	22.110589597187364
34	19.382246115816532	22.14749155172719	34.77478433840609	23.695477994050194
35	21.198150858223197	26.34433062129243	29.196017621947057	23.261500898537317
36	26.88825532108492	22.981955955242476	28.158466091906085	21.971322631766515
37	21.605715253397634	25.983020045849415	29.594482905043186	22.81678179570977
38	24.977189031525896	22.620519003242823	28.285221778219256	24.11707018701203
39	23.770545668696275	21.03171293312405	28.727286973357298	26.470454424822375
40	25.167891255500543	20.176143644649343	32.63333358608645	22.02263151376367
41	20.10486726670256	21.225700947571422	29.807553779543678	28.86187800618234
42	21.445975285800582	20.939584423331134	32.903905793859614	24.71053449700867
43	21.98850984347	21.49437750699494	32.26077549710219	24.256337152432874
44	23.6934559691439	20.417522867837924	28.7804915037041	27.10852965931408
45	20.711221985476804	19.841498522658053	27.472873272116527	31.974406219748612
46	21.753070318443648	19.819635378358775	31.281483559673745	27.145810743523835
47	18.368832508094417	22.14016171144188	35.31883541475522	24.172170365708478
48	19.57029443210167	25.548411067553324	30.396594910057807	24.484699590287203
49	24.653917799632495	20.562350401751072	31.05703879507536	23.726693003541072
50	20.95841453027098	21.510427329688632	29.738173049946543	27.792985090093847
51	19.860960512381112	22.83245248873353	27.102463584595206	30.20412341429016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1312.0
1	1493.0
2	1674.0
3	1831.5
4	1989.0
5	1654.0
6	1319.0
7	1371.5
8	1424.0
9	1698.5
10	1973.0
11	2057.5
12	2142.0
13	2404.0
14	2666.0
15	2567.5
16	2469.0
17	2472.0
18	2475.0
19	2537.0
20	2599.0
21	2446.0
22	2293.0
23	2705.5
24	3118.0
25	3467.5
26	4350.5
27	4884.0
28	5665.0
29	6446.0
30	7310.0
31	8174.0
32	9129.5
33	10085.0
34	12306.0
35	14527.0
36	15181.5
37	15836.0
38	18117.5
39	20399.0
40	22831.5
41	25264.0
42	30177.0
43	35090.0
44	40881.0
45	46672.0
46	58303.5
47	69935.0
48	82445.5
49	94956.0
50	94463.5
51	93971.0
52	83625.0
53	73279.0
54	66348.5
55	59418.0
56	55621.0
57	51824.0
58	49425.5
59	47027.0
60	37281.5
61	27536.0
62	22825.0
63	18114.0
64	15669.0
65	13224.0
66	10798.0
67	8372.0
68	6726.0
69	5080.0
70	4159.5
71	3239.0
72	2919.5
73	2600.0
74	2218.5
75	1422.0
76	1007.0
77	853.0
78	699.0
79	501.0
80	303.0
81	210.0
82	117.0
83	65.0
84	13.0
85	10.5
86	8.0
87	32.0
88	56.0
89	36.5
90	17.0
91	12.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	791286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.389061897593983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.87393791890398	20.241404246297144
2	13.151794858675522	7.730378263321465
3	5.399498272998784	4.760585668833392
4	2.78365523561357	3.272360640840351
5	1.5763198236325944	2.3163280433571374
6	1.0451270429356818	1.8429182013411667
7	0.7281843410879513	1.4980458271164743
8	0.551366075320224	1.2963305372655622
9	0.4307892921414036	1.1394443854407967
>10	4.513741974122772	30.920008255527996
>50	0.8548324088361521	16.118119339917218
>100	0.07953064077538481	4.337254507573323
>500	0.007318770623501671	1.495031424470508
>1k	0.003415426290967446	2.2822674229687903
>5k	4.87918041566778E-4	0.7495232357286923
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	5227	0.660570261574197	No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	3886	0.4910992991156169	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	3365	0.42525711310449066	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	2930	0.3702833109646828	TruSeq Adapter, Index 20 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2681	0.3388155483605169	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1031	0.13029422989917677	No Hit
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	1018	0.12865133466281473	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	1005	0.12700843942645262	No Hit
ACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	951	0.12018410536771787	TruSeq Adapter, Index 27 (95% over 22bp)
GGGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTC	941	0.11892033980128551	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAG	923	0.11664556178170725	No Hit
TGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCTT	851	0.10754644970339422	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5153635979911183	0.0
2	0.0	0.0	0.0	1.612438486211054	0.0
3	0.0	0.0	0.0	2.248491695796463	0.0
4	0.0	0.0	0.0	3.0435266136390635	0.0
5	0.0	0.0	0.0	5.044446634971426	0.0
6	0.0	0.0	0.0	6.353202255568783	0.0
7	0.0	0.0	0.0	7.4196940170810555	0.0
8	0.0	0.0	0.0	9.027330194139667	0.0
9	0.0	0.0	0.0	9.632547523904126	0.0
10	0.0	0.0	0.0	10.828322502862429	0.0
11	0.0	0.0	0.0	12.916315971721982	0.0
12	0.0	0.0	0.0	14.492989892403	0.0
13	0.0	0.0	0.0	15.19235775686667	0.0
14	0.0	0.0	0.0	15.533068953576835	0.0
15	0.0	0.0	0.0	16.069158306857446	0.0
16	0.0	0.0	0.0	17.012559302199204	0.0
17	1.2637655664323645E-4	0.0	0.0	18.09244697871566	0.0
18	1.2637655664323645E-4	0.0	0.0	19.291760501259976	0.0
19	1.2637655664323645E-4	0.0	0.0	20.08148760372356	0.0
20	1.2637655664323645E-4	0.0	0.0	20.872099342083647	0.0
21	2.527531132864729E-4	0.0	0.0	21.723245451075844	0.0
22	2.527531132864729E-4	0.0	0.0	22.585386320496003	0.0
23	2.527531132864729E-4	0.0	0.0	23.30952399006175	0.0
24	2.527531132864729E-4	0.0	0.0	23.88681210080805	0.0
25	2.527531132864729E-4	0.0	0.0	24.462078186648064	0.0
26	2.527531132864729E-4	0.0	0.0	25.020788943567812	0.0
27	2.527531132864729E-4	0.0	0.0	25.792444198431415	0.0
28	2.527531132864729E-4	0.0	0.0	26.378325915029457	0.0
29	2.527531132864729E-4	0.0	0.0	27.144673354514044	0.0
30	2.527531132864729E-4	0.0	0.0	28.07063438503904	0.0
31	2.527531132864729E-4	0.0	0.0	28.69809398877271	0.0
32	3.791296699297094E-4	0.0	0.0	29.321635919250436	0.0
33	3.791296699297094E-4	0.0	0.0	29.93987003434915	0.0
34	3.791296699297094E-4	0.0	0.0	30.722772802753997	0.0
35	3.791296699297094E-4	0.0	0.0	31.49329066860781	0.0
36	3.791296699297094E-4	0.0	0.0	32.091683664313535	0.0
37	3.791296699297094E-4	0.0	0.0	32.79913962840237	0.0
38	3.791296699297094E-4	0.0	0.0	33.74317250652735	0.0
39	3.791296699297094E-4	0.0	0.0	35.7545817820611	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGT	25	3.8896993E-5	45.0	17
TCACCCG	20	7.0319534E-4	45.0	37
CGACGTC	25	3.8896993E-5	45.0	18
CCTCGAG	25	3.8896993E-5	45.0	33
CTACGTA	20	7.0319534E-4	45.0	12
CGCTAAT	25	3.8896993E-5	45.0	25
AATTCGC	20	7.0319534E-4	45.0	18
ACGCTAA	25	3.8896993E-5	45.0	18
TGCTCGA	25	3.8896993E-5	45.0	2
TTAGTCG	20	7.0319534E-4	45.0	29
ACGTACC	20	7.0319534E-4	45.0	14
GCTACGT	20	7.0319534E-4	45.0	11
GGATTCG	20	7.0319534E-4	45.0	9
GACGACT	40	6.8102963E-9	45.0	27
CGCCGCA	20	7.0319534E-4	45.0	18
GCTAACG	20	7.0319534E-4	45.0	1
TATCGCG	20	7.0319534E-4	45.0	1
CTAGGCG	20	7.0319534E-4	45.0	1
CCCGATT	20	7.0319534E-4	45.0	13
TAATCCG	20	7.0319534E-4	45.0	28
>>END_MODULE