FastQCFastQC Report
Sat 14 Jan 2017
SRR2935116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720994
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT45980.6377306884662008TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC39520.5481321619874784TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC35670.49473365936471037TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC14100.19556334726779975TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13750.19070893793845717No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC12520.17364915658105337TruSeq Adapter, Index 22 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCTT11890.1649112197882368TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC10460.14507749024263725No Hit
AAAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC10410.1443840031955883No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG10210.14161005500739257No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC9340.1295433803887411No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG8950.12413418142175942No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG8580.11900237727359728TruSeq Adapter, Index 20 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG8470.11747670577008963TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG8220.11400927053484494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAT1400.045.0000048
GCCGATT302.1643718E-645.0000049
TACGACG351.2110831E-745.0000041
TTCATCG302.1643718E-645.00000415
ATCGACA302.1643718E-645.00000418
AAATGCG351.2110831E-745.0000041
CGGAATT207.0315355E-445.034
AGCAATC207.0315355E-445.021
TCGTTGC253.8893544E-545.043
CTCACGG502.1827873E-1145.02
CTGTACG253.8893544E-545.01
ATAGGCC207.0315355E-445.03
TCCGATC207.0315355E-445.014
CTATCCG207.0315355E-445.012
ACGCAAT207.0315355E-445.033
GACGTAT207.0315355E-445.09
CCTACGC207.0315355E-445.028
TACGGTG207.0315355E-445.01
CGCGAAT207.0315355E-445.010
GCCGATC253.8893544E-545.09
Produced by FastQC (version 0.11.5)