##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935116.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720994
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945622293666798	33.0	31.0	34.0	30.0	34.0
2	32.28578878603705	34.0	31.0	34.0	30.0	34.0
3	32.3289708374827	34.0	31.0	34.0	30.0	34.0
4	35.858857355262316	37.0	35.0	37.0	35.0	37.0
5	30.32536747878623	37.0	35.0	37.0	0.0	37.0
6	32.81276820611544	37.0	35.0	37.0	17.0	37.0
7	35.03034005830839	37.0	35.0	37.0	32.0	37.0
8	35.57761229635753	37.0	35.0	37.0	35.0	37.0
9	37.42277189546654	39.0	37.0	39.0	35.0	39.0
10	37.38705176464714	39.0	37.0	39.0	34.0	39.0
11	37.26402993644885	39.0	37.0	39.0	34.0	39.0
12	37.136114863646576	39.0	37.0	39.0	34.0	39.0
13	37.092350283081416	39.0	37.0	39.0	33.0	39.0
14	38.254475071914605	40.0	38.0	41.0	33.0	41.0
15	38.36371453853985	40.0	38.0	41.0	34.0	41.0
16	38.4190908662208	40.0	38.0	41.0	34.0	41.0
17	38.384169632479605	40.0	37.0	41.0	34.0	41.0
18	38.318880600948134	40.0	37.0	41.0	34.0	41.0
19	38.323321691997435	40.0	37.0	41.0	34.0	41.0
20	38.22459132808317	40.0	37.0	41.0	34.0	41.0
21	38.1859280382361	40.0	37.0	41.0	34.0	41.0
22	38.15296937283805	40.0	37.0	41.0	34.0	41.0
23	38.073824470106544	40.0	36.0	41.0	34.0	41.0
24	38.06221549693895	40.0	36.0	41.0	34.0	41.0
25	38.01378097459896	40.0	36.0	41.0	34.0	41.0
26	37.95918828728117	40.0	36.0	41.0	34.0	41.0
27	37.89881885286147	40.0	36.0	41.0	34.0	41.0
28	37.850824001309306	40.0	36.0	41.0	33.0	41.0
29	37.85389198800545	40.0	36.0	41.0	33.0	41.0
30	37.71812525485649	40.0	36.0	41.0	33.0	41.0
31	33.088147751576294	38.0	33.0	41.0	9.0	41.0
32	33.99817751604036	38.0	33.0	41.0	16.0	41.0
33	35.97940759562493	38.0	34.0	41.0	30.0	41.0
34	36.82024122253445	38.0	35.0	41.0	31.0	41.0
35	37.00207491324477	39.0	35.0	41.0	32.0	41.0
36	37.14215652280047	39.0	35.0	41.0	32.0	41.0
37	37.19470342332946	39.0	35.0	41.0	33.0	41.0
38	37.15874195901769	39.0	35.0	41.0	33.0	41.0
39	37.16890015728286	39.0	35.0	41.0	33.0	41.0
40	37.13712874170936	39.0	35.0	41.0	33.0	41.0
41	37.05361209663326	39.0	35.0	41.0	32.0	41.0
42	36.95991922262876	39.0	35.0	41.0	32.0	41.0
43	36.91153324438206	39.0	35.0	41.0	32.0	41.0
44	36.851382951869226	39.0	35.0	41.0	32.0	41.0
45	36.81441870528742	39.0	35.0	41.0	32.0	41.0
46	36.733444383725804	39.0	35.0	41.0	32.0	41.0
47	36.61039897696791	39.0	35.0	41.0	31.0	41.0
48	36.64104000865472	39.0	35.0	41.0	31.0	41.0
49	36.61345031997492	39.0	35.0	41.0	31.0	41.0
50	36.439251921652605	39.0	35.0	41.0	31.0	41.0
51	35.83655065090694	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	6.0
11	6.0
12	6.0
13	9.0
14	13.0
15	23.0
16	56.0
17	97.0
18	181.0
19	352.0
20	576.0
21	960.0
22	1555.0
23	2453.0
24	3439.0
25	4100.0
26	4773.0
27	5560.0
28	6506.0
29	8136.0
30	10552.0
31	14518.0
32	22262.0
33	26334.0
34	47267.0
35	70347.0
36	84130.0
37	82363.0
38	126184.0
39	198055.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.67397786944136	23.547907472184235	24.35568118458683	12.422433473787576
2	32.00553680058364	25.57497011070827	23.96940889938058	18.450084189327512
3	30.940063301497656	25.57871494076234	27.075953475340985	16.40526828239902
4	28.142536553702254	26.4780289433754	26.806048316629543	18.57338618629281
5	21.17340782308868	39.560939480772376	20.994488164950056	18.271164531188884
6	26.054724449856725	32.514278898298734	24.65873502414722	16.77226162769732
7	76.23489238468004	7.044302726513674	9.979001212215358	6.741803676590928
8	75.29979445043925	6.511843371789501	10.502445235327896	7.685916942443349
9	69.47436455781879	8.699517610410073	12.707040557896459	9.119077273874678
10	37.447052263957815	28.84018452303348	19.42193693706189	14.290826275946818
11	25.746261411329357	26.303131510109658	27.168048555189085	20.7825585233719
12	23.819893092036825	22.928346144350716	31.429387761895384	21.822373001717075
13	22.03943444744339	25.350973794511468	33.07891605200598	19.530675706039162
14	17.88627922007673	28.88678685259517	30.881255599907902	22.345678327420202
15	17.238701015542432	28.10203691015459	33.68377545444206	20.975486619860913
16	19.4731162811341	29.74671079093585	30.043107154844563	20.73706577308549
17	18.68531499568651	28.475549033695145	27.2887153013756	25.55042066924274
18	18.596271258845427	27.930745609533503	30.756289234029687	22.71669389759138
19	20.564942288007945	28.74850553541361	27.81146583744109	22.875086339137358
20	21.16896950598757	30.202331780846997	27.83809574004777	20.790602973117668
21	21.30877649467263	28.510500780866415	29.58401872969817	20.596703994762784
22	19.93040163995817	26.553064241866092	28.09662771118761	25.41990640698813
23	19.295444899680163	26.973456089787156	28.968340929328125	24.76275808120456
24	20.452597386386017	26.162353639558724	29.044624504503503	24.340424469551756
25	19.212087756624882	29.225485926373867	26.633092647095534	24.929333669905713
26	17.985863960032955	28.595383595425204	28.79677223388822	24.621980210653625
27	20.3871322091446	27.972770924584673	29.73603109041129	21.904065775859436
28	17.83745773196448	28.458073160109514	29.430342000072123	24.27412710785388
29	19.00334815546315	26.018524426000774	30.9307705750672	24.04735684346888
30	20.512375969841635	26.361939211699404	30.598590279530757	22.527094538928203
31	22.505873835288504	29.316610124356096	25.87774655544984	22.29976948490556
32	22.24720316673925	27.09481632302072	26.393978313273063	24.264002196966967
33	20.65259905075493	25.108530722863158	26.834897377786778	27.40397284859513
34	18.822902825821018	24.778569585877275	30.20815707204221	26.1903705162595
35	19.153557449853952	23.630848523011288	29.455862323403526	27.759731703731237
36	19.066316779335196	25.29119521105585	29.54559954729166	26.096888462317303
37	19.876032255469532	26.027262362793586	31.337153984637872	22.759551397099003
38	20.455093939755393	26.948213161274577	25.702016937727638	26.8946759612424
39	21.57646249483352	24.04652465901242	28.12894420758009	26.24806863857397
40	20.822919469510147	21.991583841197016	31.773496034641063	25.412000654651774
41	18.0686663134506	24.0674679678333	28.61812997056841	29.245735748147695
42	19.905990895902047	22.868983653123326	29.52326926437668	27.701756186597947
43	22.05288809615614	22.797415789867877	29.780552958831834	25.369143155144148
44	20.5628618268668	23.054144694685395	28.53463413010372	27.848359348344093
45	21.023475923516703	22.37716263935622	28.475549033695145	28.12381240343193
46	22.674807279949626	22.25288976052505	29.77805640546246	25.294246554062866
47	18.058818797382504	23.148875025312275	33.23869546764605	25.553610709659164
48	18.64661841846118	21.591441815049777	31.820375759021573	27.941564007467466
49	20.65939522381601	20.493235727343084	32.93050982393751	25.916859224903398
50	19.345514664477097	20.1122339436944	32.4371631386669	28.105088253161608
51	18.577547108575107	19.998363370568963	29.30260168600571	32.12148783485022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1707.0
1	1778.5
2	1850.0
3	1951.0
4	2052.0
5	1822.0
6	1592.0
7	1752.0
8	1912.0
9	2116.0
10	2320.0
11	2436.5
12	2553.0
13	2626.0
14	2699.0
15	2746.5
16	2794.0
17	2699.0
18	2604.0
19	2830.5
20	3057.0
21	3072.5
22	3088.0
23	3224.5
24	3361.0
25	3394.5
26	5034.5
27	6641.0
28	7175.0
29	7709.0
30	9234.0
31	10759.0
32	11228.0
33	11697.0
34	12964.0
35	14231.0
36	16693.5
37	19156.0
38	21187.5
39	23219.0
40	26264.5
41	29310.0
42	34803.0
43	40296.0
44	47270.5
45	54245.0
46	64192.5
47	74140.0
48	83535.0
49	92930.0
50	88969.5
51	85009.0
52	71604.5
53	58200.0
54	48489.0
55	38778.0
56	33621.5
57	28465.0
58	26223.5
59	23982.0
60	21514.5
61	19047.0
62	16818.5
63	14590.0
64	12884.5
65	11179.0
66	9195.5
67	7212.0
68	6275.0
69	5338.0
70	4507.0
71	3676.0
72	3281.5
73	2887.0
74	2291.0
75	1257.5
76	820.0
77	609.5
78	399.0
79	281.5
80	164.0
81	145.5
82	127.0
83	81.5
84	36.0
85	36.0
86	36.0
87	20.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	720994.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.609525461241365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.70316673871498	18.69252305879221
2	13.546962184285128	7.480503946989884
3	5.793803170503813	4.798924684603379
4	3.0960230171306975	3.4191890528023725
5	1.865347491747351	2.57506795337306
6	1.2080573296799992	2.0012333761463514
7	0.8113787112858662	1.568124683156943
8	0.5589327806596911	1.2345495070996941
9	0.4043777540716962	1.0048210107301885
>10	3.614945804329908	24.241999986829228
>50	1.2796773010043672	23.491571592471537
>100	0.10467472754282874	4.920020104375018
>500	0.0069016303874392575	1.3240100577807548
>1k	0.005751358656199381	3.2474609848493903
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT	4598	0.6377306884662008	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	3952	0.5481321619874784	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	3567	0.49473365936471037	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	1410	0.19556334726779975	TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1375	0.19070893793845717	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	1252	0.17364915658105337	TruSeq Adapter, Index 22 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCTT	1189	0.1649112197882368	TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC	1046	0.14507749024263725	No Hit
AAAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC	1041	0.1443840031955883	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1021	0.14161005500739257	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	934	0.1295433803887411	No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	895	0.12413418142175942	No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	858	0.11900237727359728	TruSeq Adapter, Index 20 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	847	0.11747670577008963	TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	822	0.11400927053484494	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6650540781199289	0.0
2	0.0	0.0	0.0	2.1481454769387818	0.0
3	0.0	0.0	0.0	3.08074685781019	0.0
4	0.0	0.0	0.0	4.059118383786828	0.0
5	0.0	0.0	0.0	6.550401251605423	0.0
6	0.0	0.0	0.0	8.144589275361515	0.0
7	0.0	0.0	0.0	9.518941905203095	0.0
8	0.0	0.0	0.0	11.694688166614425	0.0
9	0.0	0.0	0.0	12.482905544290244	0.0
10	0.0	0.0	0.0	14.079867516234533	0.0
11	0.0	0.0	0.0	16.53564384724422	0.0
12	0.0	0.0	0.0	18.427892603821945	0.0
13	0.0	0.0	0.0	19.20709464988613	0.0
14	0.0	0.0	0.0	19.5369170894626	0.0
15	0.0	0.0	0.0	20.124439315722462	0.0
16	0.0	0.0	0.0	21.262174165110945	0.0
17	0.0	0.0	0.0	22.523904498511776	0.0
18	0.0	0.0	0.0	23.96053226517835	0.0
19	1.3869740940978704E-4	0.0	0.0	24.896184989056774	0.0
20	1.3869740940978704E-4	0.0	0.0	25.685373248598463	0.0
21	1.3869740940978704E-4	0.0	0.0	26.642107978707173	0.0
22	1.3869740940978704E-4	0.0	0.0	27.515069473532375	0.0
23	2.773948188195741E-4	0.0	0.0	28.32533973930435	0.0
24	2.773948188195741E-4	0.0	0.0	28.99566431898185	0.0
25	2.773948188195741E-4	0.0	0.0	29.585683098611085	0.0
26	2.773948188195741E-4	0.0	0.0	30.09789263156143	0.0
27	2.773948188195741E-4	0.0	0.0	30.620504470217504	0.0
28	2.773948188195741E-4	0.0	0.0	31.153796009398135	0.0
29	2.773948188195741E-4	0.0	0.0	31.793884553824302	0.0
30	2.773948188195741E-4	0.0	0.0	32.468647450602916	0.0
31	2.773948188195741E-4	0.0	0.0	33.04285472555944	0.0
32	2.773948188195741E-4	0.0	0.0	33.6403631652968	0.0
33	2.773948188195741E-4	0.0	0.0	34.22608232523433	0.0
34	2.773948188195741E-4	0.0	0.0	34.836489624046806	0.0
35	2.773948188195741E-4	0.0	0.0	35.447590409906326	0.0
36	2.773948188195741E-4	0.0	0.0	36.011672773975924	0.0
37	2.773948188195741E-4	0.0	0.0	36.613064741176764	0.0
38	2.773948188195741E-4	0.0	0.0	37.1989225985237	0.0
39	2.773948188195741E-4	0.0	0.0	37.811549055886736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	140	0.0	45.000004	8
GCCGATT	30	2.1643718E-6	45.000004	9
TACGACG	35	1.2110831E-7	45.000004	1
TTCATCG	30	2.1643718E-6	45.000004	15
ATCGACA	30	2.1643718E-6	45.000004	18
AAATGCG	35	1.2110831E-7	45.000004	1
CGGAATT	20	7.0315355E-4	45.0	34
AGCAATC	20	7.0315355E-4	45.0	21
TCGTTGC	25	3.8893544E-5	45.0	43
CTCACGG	50	2.1827873E-11	45.0	2
CTGTACG	25	3.8893544E-5	45.0	1
ATAGGCC	20	7.0315355E-4	45.0	3
TCCGATC	20	7.0315355E-4	45.0	14
CTATCCG	20	7.0315355E-4	45.0	12
ACGCAAT	20	7.0315355E-4	45.0	33
GACGTAT	20	7.0315355E-4	45.0	9
CCTACGC	20	7.0315355E-4	45.0	28
TACGGTG	20	7.0315355E-4	45.0	1
CGCGAAT	20	7.0315355E-4	45.0	10
GCCGATC	25	3.8893544E-5	45.0	9
>>END_MODULE