FastQCFastQC Report
Sat 14 Jan 2017
SRR2935115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560104
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT42630.761108651250482TruSeq Adapter, Index 13 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC40440.7220087697998944TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC31610.5643594760972962TruSeq Adapter, Index 19 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18770.3351163355376858No Hit
AAAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC13560.24209789610500904No Hit
ACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC12510.22335137760130264TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC12200.21781669118592262TruSeq Adapter, Index 19 (95% over 23bp)
GCTGCAGGGAAAAGATGGGCATTCAGAGAGAAACAGCAAGACCTTCAGCCT12150.21692399982860328No Hit
TGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCTT10350.18478711096510647TruSeq Adapter, Index 19 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG9790.17478896776312972TruSeq Adapter, Index 19 (95% over 22bp)
TTATTTGGGTCTGAGTTCGAGGCCAGCCTGATTGACAAAGTGAGTTTCAGG8790.1569351406167426No Hit
GGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG7570.13515347149815035TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC7560.13497493322668647No Hit
CGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG7140.12747632582520388TruSeq Adapter, Index 13 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG7130.12729778755374002TruSeq Adapter, Index 13 (95% over 22bp)
GCAGAGGCAGGTGGATTTCTGAGTTGGAGGCCAGCCTGGTCTACAGAGTGA6830.12194163940982389No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT6560.11712110608029937No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC6480.1156927999085884No Hit
ACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG6090.10872980732149745TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG351.2104283E-745.0000041
AATCACG302.1635005E-645.0000041
ACCATAC302.1635005E-645.00000416
GACGTAT302.1635005E-645.0000042
ATTACGC302.1635005E-645.00000440
CGCGCAT700.045.00000436
GTCTACG453.8380676E-1045.0000041
CCGCTTG351.2104283E-745.00000439
CCTCGAA351.2104283E-745.00000420
GGACGTA302.1635005E-645.0000041
CTCGCGC453.8380676E-1045.00000433
GTATCAA351.2104283E-745.0000049
ACGTATT302.1635005E-645.0000043
GCGCTCC302.1635005E-645.00000436
TTCGCCG351.2104283E-745.00000435
TAGTCTC453.8380676E-1045.00000429
ACGAAAG351.2104283E-745.0000041
ACGCGCA700.045.00000435
CGGACAT253.8882357E-545.021
AACGTTA207.030187E-445.023
Produced by FastQC (version 0.11.5)