##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935115.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560104
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84251139074172	33.0	31.0	34.0	30.0	34.0
2	32.15548540985245	34.0	31.0	34.0	30.0	34.0
3	32.21970919686344	34.0	31.0	34.0	30.0	34.0
4	35.77084077242798	37.0	35.0	37.0	35.0	37.0
5	30.334762829760187	37.0	35.0	37.0	0.0	37.0
6	32.776064802251014	37.0	35.0	37.0	17.0	37.0
7	34.922819690628884	37.0	35.0	37.0	32.0	37.0
8	35.47560274520446	37.0	35.0	37.0	33.0	37.0
9	37.28805186179709	39.0	37.0	39.0	34.0	39.0
10	37.23754338479996	39.0	37.0	39.0	34.0	39.0
11	37.13550340651022	39.0	37.0	39.0	34.0	39.0
12	36.934362189879025	39.0	37.0	39.0	33.0	39.0
13	36.87304322054476	39.0	37.0	39.0	33.0	39.0
14	38.006834445031636	40.0	37.0	41.0	33.0	41.0
15	38.11783347378344	40.0	37.0	41.0	33.0	41.0
16	38.192026837872966	40.0	37.0	41.0	34.0	41.0
17	38.15927399197292	40.0	37.0	41.0	34.0	41.0
18	38.08492172882179	40.0	37.0	41.0	34.0	41.0
19	38.03964442317855	40.0	36.0	41.0	34.0	41.0
20	37.949995000928396	40.0	36.0	41.0	34.0	41.0
21	37.89613178981046	40.0	36.0	41.0	33.0	41.0
22	37.83412366274835	40.0	36.0	41.0	33.0	41.0
23	37.78891955779641	39.0	36.0	41.0	33.0	41.0
24	37.75193714024538	39.0	36.0	41.0	33.0	41.0
25	37.6925678088355	39.0	35.0	41.0	33.0	41.0
26	37.63479818033794	39.0	35.0	41.0	33.0	41.0
27	37.589160227386344	39.0	35.0	41.0	33.0	41.0
28	37.51321897361918	39.0	35.0	41.0	33.0	41.0
29	37.45984852813049	39.0	35.0	41.0	33.0	41.0
30	37.29314555868196	39.0	35.0	41.0	33.0	41.0
31	32.799665776355816	38.0	33.0	40.0	9.0	41.0
32	33.60398247468327	38.0	33.0	40.0	16.0	41.0
33	35.43739019896305	38.0	33.0	40.0	27.0	41.0
34	36.30440418208047	38.0	35.0	40.0	30.0	41.0
35	36.41872045191607	39.0	35.0	40.0	31.0	41.0
36	36.61397883250253	39.0	35.0	40.0	31.0	41.0
37	36.67409623926985	39.0	35.0	41.0	31.0	41.0
38	36.58663748161056	39.0	35.0	41.0	31.0	41.0
39	36.585050276377245	39.0	35.0	41.0	31.0	41.0
40	36.53392048619542	39.0	35.0	41.0	31.0	41.0
41	36.49467420336223	39.0	35.0	41.0	31.0	41.0
42	36.42507284361476	39.0	35.0	41.0	31.0	41.0
43	36.38761908502707	39.0	35.0	41.0	31.0	41.0
44	36.29633068144487	39.0	35.0	41.0	30.0	41.0
45	36.26127112107751	39.0	35.0	41.0	30.0	41.0
46	36.2061420736149	39.0	35.0	41.0	30.0	41.0
47	36.11626947852542	39.0	35.0	40.0	30.0	41.0
48	36.13388406438804	39.0	35.0	40.0	30.0	41.0
49	36.1189386256838	39.0	35.0	40.0	30.0	41.0
50	35.939932583948696	39.0	35.0	40.0	30.0	41.0
51	35.324943224829674	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	5.0
11	1.0
12	6.0
13	11.0
14	11.0
15	27.0
16	44.0
17	112.0
18	215.0
19	380.0
20	669.0
21	1043.0
22	1668.0
23	2659.0
24	3655.0
25	4249.0
26	4701.0
27	5315.0
28	6180.0
29	7522.0
30	9427.0
31	13245.0
32	19575.0
33	22608.0
34	40045.0
35	57147.0
36	63848.0
37	63069.0
38	99114.0
39	133463.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.934704983360234	23.35905474697556	22.676145858626253	11.03009441103795
2	29.901946781312045	25.872337994372472	24.389042035050636	19.83667318926485
3	31.40863125419565	26.784311485009926	25.503835002071046	16.30322225872338
4	28.948552411694973	28.255466841872224	24.31405596103581	18.481924785397
5	21.097689000614174	40.4996214988645	20.362646936997415	18.04004256352392
6	25.12586948138203	34.44271063945267	24.47331209918158	15.958107779983719
7	73.70166968991472	9.158834787825118	10.301122648650965	6.838372873609187
8	72.50689157727851	7.575914473026438	11.459300415637095	8.45789353405796
9	67.41533715167182	9.898518846499936	13.797080542185022	8.889063459643209
10	38.21093939696914	25.385464128090497	21.046448516704043	15.357147958236329
11	27.93052718780798	27.441689400539897	23.725772356562352	20.90201105508977
12	23.779869452815905	25.166219130732863	31.101545427277795	19.95236598917344
13	22.271578135489122	28.35955465413566	31.282047619727766	18.08681959064745
14	18.491387313784582	31.027630582891746	29.535050633453785	20.94593146986988
15	16.824554011397883	29.87409481096368	33.59108308457001	19.710268093068432
16	19.081456300972675	28.450966249125166	31.478261180066557	20.9893162698356
17	18.188229328838933	28.383300244240356	27.27279219573508	26.155678231185636
18	19.95343721880222	28.76305114764401	29.79339551226201	21.49011612129176
19	20.75025352434548	29.831424164083813	26.655942467827405	22.762379843743304
20	20.31640552468827	30.518260894405323	28.143523345664377	21.02181023524203
21	20.95414423035722	31.15814205933184	28.592904174938937	19.294809535372
22	18.89988287889392	27.422942882036196	27.433655178324024	26.24351906074586
23	18.164483738734237	28.25011069372831	28.27421336037593	25.311192207161525
24	20.729721623127134	27.1417451044806	27.47079113878851	24.65774213360376
25	17.984517161098655	30.992637081684833	25.0	26.022845757216515
26	18.525309585362717	29.493451216202704	27.11139359833174	24.86984560010284
27	21.98395298016083	28.647715424278346	27.70824703983546	21.660084555725366
28	18.974511876365817	28.606473083570194	27.561310042420693	24.857704997643296
29	17.709568223044293	28.941232342564955	27.172632225443845	26.17656720894691
30	20.578142630654305	29.57861397169097	27.18602259580364	22.657220801851086
31	24.160691585848344	30.64823675603102	21.634196506363104	23.55687515175753
32	19.006827303500778	33.72409409681059	22.25479553797152	25.01428306171711
33	18.87738705668947	32.525031065659235	23.885742647793982	24.71183922985731
34	19.196256409523947	27.97676860011712	24.906267407481465	27.920707582877462
35	16.37963663890992	31.045662948309598	24.490987388056503	28.083713024723977
36	20.418886492508534	28.054968363018297	25.74396897718995	25.782176167283215
37	19.524231214203077	28.45935754788396	28.11977775555968	23.896633482353277
38	19.511554996929142	32.34488594975219	24.238891348749515	23.904667704569153
39	20.91593704026395	28.26957136531787	24.99678631111365	25.81770528330453
40	20.963963835287732	26.250124976790023	27.54024252638796	25.24566866153429
41	18.48960193106994	27.039085598388873	24.769685629811605	29.701626840729578
42	21.066266264836532	23.737912959021894	27.29582363275392	27.899997143387655
43	22.992515675660236	24.44135374858955	27.239584077242796	25.32654649850742
44	20.61938497136246	25.68130204390613	26.177638438575695	27.521674546155715
45	19.682951737534456	24.429213146130007	27.45740076842872	28.430434347906818
46	22.61222915744219	25.047312641937925	27.73467070401211	24.60578749660777
47	17.415694228214758	24.78414722980018	31.051197634724982	26.748960907260077
48	18.778298316027023	23.051433305243314	29.369367117535315	28.80090126119435
49	20.698477425620958	21.36656763743876	31.665904903375086	26.269050033565193
50	19.35176324396898	21.233913701741105	31.24223358519132	28.172089469098594
51	18.072536528930343	22.06108151343322	27.45418707954237	32.41219487809407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2217.0
1	2016.5
2	1816.0
3	1874.5
4	1933.0
5	1706.0
6	1479.0
7	1620.0
8	1761.0
9	2001.5
10	2242.0
11	2330.5
12	2419.0
13	2626.5
14	2834.0
15	2776.5
16	2719.0
17	2731.5
18	2744.0
19	2706.0
20	2668.0
21	2321.5
22	1975.0
23	1953.0
24	1931.0
25	2629.0
26	3168.0
27	3009.0
28	3744.0
29	4479.0
30	4339.0
31	4199.0
32	5465.0
33	6731.0
34	8562.5
35	10394.0
36	11662.5
37	12931.0
38	14498.0
39	16065.0
40	18518.0
41	20971.0
42	24815.5
43	28660.0
44	35757.0
45	42854.0
46	52847.0
47	62840.0
48	71435.5
49	80031.0
50	77813.5
51	75596.0
52	61988.5
53	48381.0
54	39326.0
55	30271.0
56	26733.5
57	23196.0
58	19563.5
59	15931.0
60	14357.5
61	12784.0
62	11315.5
63	9847.0
64	8326.0
65	6805.0
66	5705.0
67	4605.0
68	3590.5
69	2576.0
70	2307.5
71	2039.0
72	1465.5
73	892.0
74	983.0
75	690.0
76	306.0
77	238.5
78	171.0
79	229.5
80	288.0
81	196.5
82	105.0
83	56.0
84	7.0
85	4.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.471882727692293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.86219975361517	20.294986755094673
2	12.723008433213048	7.499420249741901
3	5.700310700123975	5.0399666539639
4	3.0575935230033346	3.604521509556232
5	1.8465770533100268	2.7211051181400356
6	1.2703955090223125	2.246456847581553
7	0.8253849276808843	1.7027953455671065
8	0.6136530465681944	1.4468408499159138
9	0.46830829193274925	1.2421734354223086
>10	3.5540235350260616	24.39584744431088
>50	0.9556380239961267	18.70494038437922
>100	0.10909740447354625	5.536634677725374
>500	0.00759538891904436	1.6119264631257648
>1k	0.00621440911558175	3.952384265475143
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	4263	0.761108651250482	TruSeq Adapter, Index 13 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	4044	0.7220087697998944	TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	3161	0.5643594760972962	TruSeq Adapter, Index 19 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1877	0.3351163355376858	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	1356	0.24209789610500904	No Hit
ACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1251	0.22335137760130264	TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1220	0.21781669118592262	TruSeq Adapter, Index 19 (95% over 23bp)
GCTGCAGGGAAAAGATGGGCATTCAGAGAGAAACAGCAAGACCTTCAGCCT	1215	0.21692399982860328	No Hit
TGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCTT	1035	0.18478711096510647	TruSeq Adapter, Index 19 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	979	0.17478896776312972	TruSeq Adapter, Index 19 (95% over 22bp)
TTATTTGGGTCTGAGTTCGAGGCCAGCCTGATTGACAAAGTGAGTTTCAGG	879	0.1569351406167426	No Hit
GGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	757	0.13515347149815035	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC	756	0.13497493322668647	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	714	0.12747632582520388	TruSeq Adapter, Index 13 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	713	0.12729778755374002	TruSeq Adapter, Index 13 (95% over 22bp)
GCAGAGGCAGGTGGATTTCTGAGTTGGAGGCCAGCCTGGTCTACAGAGTGA	683	0.12194163940982389	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	656	0.11712110608029937	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	648	0.1156927999085884	No Hit
ACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	609	0.10872980732149745	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8116349820747576	0.0
2	0.0	0.0	0.0	2.6595060917258224	0.0
3	0.0	0.0	0.0	3.7578735377715566	0.0
4	0.0	0.0	0.0	4.951937497321926	0.0
5	0.0	0.0	0.0	7.841043806150286	0.0
6	0.0	0.0	0.0	9.725693799722908	0.0
7	0.0	0.0	0.0	11.225772356562352	0.0
8	0.0	0.0	0.0	13.752088897776128	0.0
9	0.0	0.0	0.0	14.551226200848413	0.0
10	0.0	0.0	0.0	16.260551611843514	0.0
11	0.0	0.0	0.0	18.949159441817947	0.0
12	0.0	0.0	0.0	20.967177524174083	0.0
13	0.0	0.0	0.0	21.813984545727223	0.0
14	0.0	0.0	0.0	22.15784925656664	0.0
15	0.0	0.0	0.0	22.768450144973077	0.0
16	1.78538271463871E-4	0.0	0.0	24.04856955136903	0.0
17	1.78538271463871E-4	0.0	0.0	25.58399868595832	0.0
18	1.78538271463871E-4	0.0	0.0	27.302251010526618	0.0
19	1.78538271463871E-4	0.0	0.0	28.32902460971534	0.0
20	1.78538271463871E-4	0.0	0.0	29.212610515190036	0.0
21	1.78538271463871E-4	0.0	0.0	30.12922600088555	0.0
22	1.78538271463871E-4	0.0	0.0	31.124576864296632	0.0
23	3.57076542927742E-4	0.0	0.0	32.03262251296188	0.0
24	3.57076542927742E-4	0.0	0.0	32.75820204819105	0.0
25	3.57076542927742E-4	0.0	0.0	33.420579035322014	0.0
26	5.35614814391613E-4	0.0	0.0	34.025288160770145	0.0
27	5.35614814391613E-4	0.0	0.0	34.56286689614786	0.0
28	5.35614814391613E-4	0.0	0.0	35.14882950309228	0.0
29	5.35614814391613E-4	0.0	0.0	35.80906403096568	0.0
30	5.35614814391613E-4	0.0	0.0	36.4632282576093	0.0
31	7.14153085855484E-4	0.0	0.0	37.08882636081871	0.0
32	7.14153085855484E-4	0.0	0.0	37.72656506648765	0.0
33	7.14153085855484E-4	0.0	0.0	38.376087298073216	0.0
34	7.14153085855484E-4	0.0	0.0	38.963299672917884	0.0
35	7.14153085855484E-4	0.0	0.0	39.61246482796052	0.0
36	7.14153085855484E-4	0.0	0.0	40.23199262994015	0.0
37	7.14153085855484E-4	0.0	0.0	40.857412194878094	0.0
38	7.14153085855484E-4	0.0	0.0	41.4617642437833	0.0
39	7.14153085855484E-4	0.0	0.0	42.06361675688801	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	35	1.2104283E-7	45.000004	1
AATCACG	30	2.1635005E-6	45.000004	1
ACCATAC	30	2.1635005E-6	45.000004	16
GACGTAT	30	2.1635005E-6	45.000004	2
ATTACGC	30	2.1635005E-6	45.000004	40
CGCGCAT	70	0.0	45.000004	36
GTCTACG	45	3.8380676E-10	45.000004	1
CCGCTTG	35	1.2104283E-7	45.000004	39
CCTCGAA	35	1.2104283E-7	45.000004	20
GGACGTA	30	2.1635005E-6	45.000004	1
CTCGCGC	45	3.8380676E-10	45.000004	33
GTATCAA	35	1.2104283E-7	45.000004	9
ACGTATT	30	2.1635005E-6	45.000004	3
GCGCTCC	30	2.1635005E-6	45.000004	36
TTCGCCG	35	1.2104283E-7	45.000004	35
TAGTCTC	45	3.8380676E-10	45.000004	29
ACGAAAG	35	1.2104283E-7	45.000004	1
ACGCGCA	70	0.0	45.000004	35
CGGACAT	25	3.8882357E-5	45.0	21
AACGTTA	20	7.030187E-4	45.0	23
>>END_MODULE