##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935114.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2230711
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21946679780572	33.0	31.0	34.0	30.0	34.0
2	32.573258481264496	34.0	31.0	34.0	31.0	34.0
3	32.57184637543814	34.0	31.0	34.0	31.0	34.0
4	36.081122565854564	37.0	35.0	37.0	35.0	37.0
5	30.51368554689514	37.0	35.0	37.0	0.0	37.0
6	33.02115693157921	37.0	35.0	37.0	17.0	37.0
7	35.21220005639458	37.0	35.0	37.0	32.0	37.0
8	35.7878958771441	37.0	35.0	37.0	35.0	37.0
9	37.71784735898106	39.0	38.0	39.0	35.0	39.0
10	37.5967290249611	39.0	37.0	39.0	35.0	39.0
11	37.48015677512685	39.0	37.0	39.0	35.0	39.0
12	37.42277596694507	39.0	37.0	39.0	35.0	39.0
13	37.45116557008057	39.0	37.0	39.0	35.0	39.0
14	38.78786808331514	40.0	38.0	41.0	35.0	41.0
15	38.85130480819792	40.0	38.0	41.0	35.0	41.0
16	38.89330397348648	40.0	38.0	41.0	35.0	41.0
17	38.86681062674636	40.0	38.0	41.0	35.0	41.0
18	38.742875253674725	40.0	38.0	41.0	35.0	41.0
19	38.64618545387547	40.0	37.0	41.0	35.0	41.0
20	38.48474186033063	40.0	37.0	41.0	35.0	41.0
21	38.42366671433458	40.0	37.0	41.0	35.0	41.0
22	38.408816740492156	40.0	37.0	41.0	35.0	41.0
23	38.3543417322997	40.0	37.0	41.0	34.0	41.0
24	38.32677652999425	40.0	37.0	41.0	34.0	41.0
25	38.27933963655534	40.0	37.0	41.0	34.0	41.0
26	38.205100974532336	40.0	36.0	41.0	34.0	41.0
27	38.16087740635161	40.0	36.0	41.0	34.0	41.0
28	38.082633294944976	40.0	36.0	41.0	34.0	41.0
29	38.02954842648823	40.0	36.0	41.0	34.0	41.0
30	37.85518697850147	40.0	36.0	41.0	34.0	41.0
31	33.185161143689164	39.0	33.0	41.0	9.0	41.0
32	33.99873313934436	39.0	33.0	41.0	16.0	41.0
33	35.73184693131473	39.0	34.0	41.0	28.0	41.0
34	36.41396397830109	39.0	35.0	41.0	30.0	41.0
35	36.487216855971035	39.0	35.0	41.0	30.0	41.0
36	36.59452434672174	39.0	35.0	41.0	31.0	41.0
37	36.69633179735071	40.0	35.0	41.0	31.0	41.0
38	36.672390103424426	40.0	35.0	41.0	31.0	41.0
39	36.6504405994322	40.0	35.0	41.0	31.0	41.0
40	36.65355574971388	40.0	35.0	41.0	31.0	41.0
41	36.58076460823477	40.0	35.0	41.0	31.0	41.0
42	36.464039940628794	40.0	35.0	41.0	30.0	41.0
43	36.45090152870542	39.0	35.0	41.0	30.0	41.0
44	36.3899788004811	39.0	35.0	41.0	30.0	41.0
45	36.341765472981486	39.0	35.0	41.0	30.0	41.0
46	36.292478945053844	39.0	35.0	41.0	30.0	41.0
47	36.21251834056496	39.0	35.0	41.0	30.0	41.0
48	36.15954868201215	39.0	35.0	41.0	30.0	41.0
49	36.11206920125467	39.0	35.0	41.0	30.0	41.0
50	35.9306839837164	39.0	35.0	41.0	29.0	41.0
51	35.369813929280845	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	8.0
11	17.0
12	31.0
13	19.0
14	36.0
15	55.0
16	115.0
17	210.0
18	417.0
19	739.0
20	1383.0
21	2393.0
22	4014.0
23	7561.0
24	13559.0
25	20507.0
26	27403.0
27	29531.0
28	28594.0
29	28388.0
30	31402.0
31	39581.0
32	57519.0
33	69619.0
34	123438.0
35	185029.0
36	247983.0
37	251686.0
38	368357.0
39	690333.0
40	777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.4356377854415	20.876572536738287	24.303596476639065	16.384193201181148
2	36.84004785918032	22.96402357813271	24.096218649569575	16.099709913117387
3	29.622393936283096	22.4388098682438	31.438810316531367	16.499985878941736
4	26.411623917217426	24.800881871295744	31.07049725401453	17.716996957472304
5	21.266627546105255	38.941395815056275	24.32107969163195	15.47089694720652
6	25.5404218654949	32.35331694692858	27.42493312670265	14.681328060873867
7	77.82805571855789	5.297100341550295	12.351084474860258	4.523759465031553
8	80.52078462875738	3.9180781374189664	11.72298876905166	3.838148464771994
9	74.47598545934457	6.303774895089504	13.654973683278559	5.565265962287361
10	38.048048357676095	26.202094309841122	21.31360808280409	14.43624924967869
11	26.218949922244523	25.16619140713432	29.977572173177077	18.637286497444087
12	23.72535034793839	22.85257928974215	32.857550798826026	20.564519563493434
13	22.10371491421345	23.151990553684453	34.80110153220207	19.94319299990003
14	20.215079407417637	25.24329686812859	34.13476689719108	20.406856827262697
15	19.73455996765157	26.59327003811789	34.550643270239846	19.12152672399069
16	22.663536424037	26.07316680645767	32.609827091003716	18.653469678501608
17	22.56051994184814	26.093698376885214	31.005719701028056	20.340061980238588
18	22.197944960149478	25.80459772691308	32.04570202056654	19.95175529237091
19	21.91449273348273	27.910966503504937	30.197008935716013	19.977531827296318
20	22.531515736462502	27.181871609545116	30.966673854210608	19.319938799781774
21	22.354397319957627	27.191778764707752	31.853386655644773	18.600437259689848
22	21.852135933341433	24.562392887290198	31.86849394654888	21.716977232819488
23	20.857654801540853	25.111589981848837	32.27917018385617	21.751585032754132
24	20.971519842776583	24.92882314203857	33.144634154760524	20.95502286042432
25	21.24744083836947	27.201416947332042	30.37632396128409	21.174818253014397
26	20.53161525630169	28.09117810420086	30.336202224313237	21.04100441518422
27	20.727875551785953	27.052226846059398	30.65408293588905	21.5658146662656
28	20.358621085384883	26.759943354383424	31.182345001212617	21.699090559019073
29	20.92682557265374	26.645540368070986	30.420525115086626	22.007108944188648
30	21.425904117566102	27.771235269831006	29.988062102172808	20.814798510430084
31	24.277954427982827	28.16989740042525	27.178240480277367	20.373907691314564
32	23.05031893418735	28.042269930977167	28.19796020192665	20.709450932908837
33	22.819674982550406	28.547848645566372	27.947501939964432	20.684974431918793
34	21.77906506042244	28.326977362822888	28.336077600370462	21.55787997638421
35	22.47718328371537	28.584742712077	27.420181278525096	21.51789272568253
36	21.885219555558745	30.840839535018205	26.85305268140965	20.4208882280134
37	22.687116349899203	29.13788473719814	27.83686456918893	20.338134343713733
38	22.511701426137225	28.339708729638218	27.870217163944588	21.278372680279965
39	22.221390399742504	28.314783941084254	27.43228504275094	22.031540616422298
40	21.92798618915673	26.966828065132596	29.268695048350057	21.836490697360617
41	20.785883962557232	28.3684439624855	28.4072208367646	22.438451238192666
42	21.06830512782696	28.6302438998149	28.23539221351399	22.066058758844154
43	21.576349424017724	27.28376737282418	29.444827232214305	21.695055970943795
44	22.23089409609761	26.71260418763345	28.0561220167023	23.00037969956664
45	21.621940269268407	26.47254619715418	27.923428897781914	23.98208463579549
46	21.67613823574636	27.357286533307096	27.997754975879886	22.968820255066657
47	20.862272163449234	27.33115136833055	29.358397389890488	22.448179078329733
48	20.651352864624776	26.6134429784943	29.981248131201216	22.753956025679706
49	21.889612773685162	26.048197189147317	29.48893872850405	22.573251308663473
50	20.301240277203096	25.98458518382704	30.153390555746572	23.560783983223285
51	20.363686734857183	25.668811423801646	29.082969510617918	24.884532330723253
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1009.0
1	1564.5
2	2120.0
3	17814.5
4	33509.0
5	24626.0
6	15743.0
7	14564.0
8	13385.0
9	13369.0
10	13353.0
11	13483.5
12	13614.0
13	13441.5
14	13269.0
15	12744.5
16	12220.0
17	11599.0
18	10978.0
19	10777.5
20	10577.0
21	10805.0
22	11033.0
23	12466.0
24	13899.0
25	15313.0
26	19434.0
27	22141.0
28	26441.5
29	30742.0
30	35878.5
31	41015.0
32	45413.0
33	49811.0
34	56970.0
35	64129.0
36	70201.0
37	76273.0
38	82235.0
39	88197.0
40	95628.5
41	103060.0
42	111512.5
43	119965.0
44	132725.5
45	145486.0
46	167151.0
47	188816.0
48	201876.5
49	214937.0
50	205068.5
51	195200.0
52	174622.5
53	154045.0
54	137207.5
55	120370.0
56	111243.5
57	102117.0
58	94402.0
59	86687.0
60	80499.5
61	74312.0
62	66563.0
63	58814.0
64	49657.0
65	40500.0
66	33682.5
67	26865.0
68	22183.5
69	17502.0
70	14630.0
71	11758.0
72	9661.5
73	7565.0
74	5706.5
75	3036.5
76	2225.0
77	1986.5
78	1748.0
79	1208.5
80	669.0
81	490.0
82	311.0
83	213.0
84	115.0
85	81.5
86	48.0
87	25.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2230711.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.580463923099426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.44337640950317	23.070934505276224
2	9.749567527493953	5.96292596080701
3	3.4075580386174016	3.126141169974207
4	1.5870734873957866	1.9413377409845778
5	0.9745893340326548	1.490169698461155
6	0.6839156369129624	1.2548674476636248
7	0.5668215816095881	1.2133566849072317
8	0.4517457144273192	1.1051674817967527
9	0.37177686217936645	1.02321980291873
>10	6.337158141088067	47.883231021189424
>50	0.39383569415966946	7.1599857843348085
>100	0.029160566871712812	1.6195734067842533
>500	0.0013032402698661624	0.24749142766634752
>1k	0.0016290503373327032	1.2660521623817542
>5k	3.258100674665406E-4	0.7469578233553169
>10k+	1.629050337332703E-4	0.8885878814985185
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17837	0.7996105277644661	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTT	7883	0.35338508663829604	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC	7111	0.3187772867036564	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCT	4165	0.18671177037276457	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTG	3580	0.16048694788343268	No Hit
CTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGCT	2933	0.13148274249779554	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	2752	0.12336873759083986	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCC	2575	0.11543404770945227	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCT	2434	0.10911319305817742	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13861051476412678	0.0
2	0.0	0.0	0.0	0.43622862845074956	0.0
3	0.0	0.0	0.0	0.7616405711004249	0.0
4	0.0	0.0	0.0	1.1365434608068907	0.0
5	0.0	0.0	0.0	2.201271253873765	0.0
6	0.0	0.0	0.0	3.553216889144313	0.0
7	0.0	0.0	0.0	4.410656512654485	0.0
8	0.0	0.0	0.0	5.5281477519947675	0.0
9	0.0	0.0	0.0	6.071696423248014	0.0
10	0.0	0.0	0.0	6.871127635986912	0.0
11	0.0	0.0	0.0	8.002650276077896	0.0
12	4.4828756392020305E-5	0.0	0.0	8.803516009021338	0.0
13	4.4828756392020305E-5	0.0	0.0	9.1705738663592	0.0
14	4.4828756392020305E-5	0.0	0.0	9.333122937036666	4.4828756392020305E-5
15	8.965751278404061E-5	0.0	0.0	9.566591100326308	4.4828756392020305E-5
16	8.965751278404061E-5	0.0	0.0	10.034872289597352	4.4828756392020305E-5
17	8.965751278404061E-5	0.0	0.0	10.628270537958524	4.4828756392020305E-5
18	2.689725383521218E-4	0.0	0.0	11.241796897939714	4.4828756392020305E-5
19	2.689725383521218E-4	0.0	0.0	11.668925288842885	4.4828756392020305E-5
20	2.689725383521218E-4	0.0	0.0	12.041003966896653	4.4828756392020305E-5
21	2.689725383521218E-4	0.0	0.0	12.504264335451792	4.4828756392020305E-5
22	2.689725383521218E-4	0.0	0.0	13.015222500808038	4.4828756392020305E-5
23	2.689725383521218E-4	0.0	0.0	13.548639873116688	4.4828756392020305E-5
24	2.689725383521218E-4	0.0	0.0	13.96805771792043	4.4828756392020305E-5
25	2.689725383521218E-4	0.0	0.0	14.35215050268726	4.4828756392020305E-5
26	2.689725383521218E-4	0.0	0.0	14.699349220943457	4.4828756392020305E-5
27	2.689725383521218E-4	0.0	0.0	15.033637257358752	4.4828756392020305E-5
28	2.689725383521218E-4	0.0	0.0	15.395584636467925	4.4828756392020305E-5
29	2.689725383521218E-4	0.0	0.0	15.779856736260323	4.4828756392020305E-5
30	2.689725383521218E-4	0.0	0.0	16.18076030467416	4.4828756392020305E-5
31	2.689725383521218E-4	0.0	0.0	16.57126360160505	4.4828756392020305E-5
32	2.689725383521218E-4	0.0	0.0	17.006595654927956	4.4828756392020305E-5
33	3.138012947441421E-4	0.0	0.0	17.440582845559106	4.4828756392020305E-5
34	3.138012947441421E-4	0.0	0.0	17.84937627509794	4.4828756392020305E-5
35	3.138012947441421E-4	0.0	0.0	18.266821654620433	4.4828756392020305E-5
36	3.5863005113616244E-4	0.0	0.0	18.6934121004469	4.4828756392020305E-5
37	3.5863005113616244E-4	0.0	0.0	19.128295866205885	4.4828756392020305E-5
38	3.5863005113616244E-4	0.0	0.0	19.588956166890288	4.4828756392020305E-5
39	4.034588075281827E-4	0.0	0.0	20.046926742191168	4.4828756392020305E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	2005	0.0	43.653366	1
CGTTTTT	12400	0.0	43.493954	1
CACGCGA	30	1.1402662E-4	37.500004	32
CGTTATC	145	0.0	37.24138	1
ACGGGTA	415	0.0	36.86747	5
CGAATAT	135	0.0	36.666664	14
AATACGG	270	0.0	36.666664	2
TAACGGG	695	0.0	36.582733	3
GCGTAAG	185	0.0	36.486485	1
GTATACG	105	0.0	36.42857	1
CGTTTCT	935	0.0	35.855614	1
TCTAGCG	95	0.0	35.526314	1
TACGGGA	1155	0.0	35.259743	4
CGTTCTG	940	0.0	35.18617	1
TAAGTCG	45	8.70481E-7	35.000004	1
GTTATTT	2565	0.0	35.0	2
CGTTAGG	240	0.0	34.687504	2
TAAGGGA	2385	0.0	34.62264	4
GCGTTAG	130	0.0	34.615387	1
CTTACGC	85	0.0	34.411762	29
>>END_MODULE