SRR2935113.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935113.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2670220
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	27622	1.0344465999056256	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	23379	0.8755458351746298	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	15131	0.5666574289758897	RNA PCR Primer, Index 30 (95% over 21bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	13462	0.5041532158398934	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT	8947	0.33506602452232404	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	8886	0.33278156855989394	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	7940	0.2973537760933556	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	6107	0.22870774692721946	RNA PCR Primer, Index 30 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	5923	0.22181692894218452	RNA PCR Primer, Index 30 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	4661	0.17455490558830358	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	4290	0.16066091932499943	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	3968	0.14860198785118828	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATG	3843	0.1439207256330939	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCATC	3840	0.14380837533985963	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	3746	0.14028806615185266	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	2910	0.10897978443723738	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAT	20	7.0349657E-4	45.0	10
CGGTTAT	20	7.0349657E-4	45.0	31
CGTTATA	20	7.0349657E-4	45.0	11
AGCGTAC	25	3.892199E-5	44.999996	32
CGTACGA	30	2.1665855E-6	44.999996	11
TACGTCA	25	3.892199E-5	44.999996	36
CTCAACT	5505	0.0	43.814713	2
CCTGCAT	4430	0.0	42.81603	13
TAATACG	1035	0.0	42.608696	4
TCAACTT	5550	0.0	42.608105	3
CATTAGA	4300	0.0	42.5407	17
CTTGACC	5575	0.0	42.295963	7
TTGACCC	5075	0.0	42.16256	8
ACTTGAC	5590	0.0	42.14222	6
AACTTGA	5540	0.0	41.87274	5
TGCATTA	4405	0.0	41.833145	15
GCTCAAC	5990	0.0	41.50668	1
ATTAGAA	4400	0.0	41.164772	18
GCATTAG	4535	0.0	41.080486	16
CAACTTG	5685	0.0	40.883907	4