FastQCFastQC Report
Sat 14 Jan 2017
SRR2935112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974157
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC324821.0921414034296104No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG270230.9085935947564301No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC253060.8508629504091412TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC249280.8381534666798021No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT195330.6567575282676738TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC169720.5706490948527599TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTT98520.33125352831071125No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC74280.2497514421733621TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT65120.21895279906205353No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC63700.21417833692034416No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT56210.18899473027146851No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA55690.18724633568436366No Hit
ACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC54800.18425389110258805TruSeq Adapter, Index 16 (95% over 22bp)
TGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCTT53310.17924406815107607TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC47290.15900303850805456No Hit
GCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG42390.14252778182187423No Hit
ACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG38370.1290113467446406TruSeq Adapter, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG37160.12494296703233891TruSeq Adapter, Index 16 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG36610.12309370352674724No Hit
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG31890.10722366035148784TruSeq Adapter, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC30120.10127239416076554No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG207.035096E-445.013
CCCGTTA207.035096E-445.01
CGCTATA207.035096E-445.027
TCGCGAC207.035096E-445.033
TATCGCG1000.042.7500041
TCTAACG4700.042.60638428
TAATACG11900.042.352944
CGCCTAA5750.041.4782626
ACGTAAG1700.041.029411
GCCGATA501.0822987E-940.5000049
ACGCCTA6000.040.525
AATACGG12600.040.05
ACGATAG850.039.7058831
CTTACGC3500.039.21428729
TTACGGG7900.039.0189863
TACGGGA10600.038.844344
ATAATAC17850.038.6974753
GACGCGT3900.038.65384726
CGTACTA356.2516356E-638.57142644
TACGGCT47650.038.247647
Produced by FastQC (version 0.11.5)