##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935112.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2974157
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.698346455819244	33.0	31.0	34.0	30.0	34.0
2	32.01406179969652	33.0	31.0	34.0	30.0	34.0
3	32.10172395068586	33.0	31.0	34.0	30.0	34.0
4	35.70498699295296	37.0	35.0	37.0	33.0	37.0
5	30.234712222656707	37.0	35.0	37.0	0.0	37.0
6	32.67468227131251	37.0	33.0	37.0	17.0	37.0
7	34.891930049422406	37.0	35.0	37.0	32.0	37.0
8	35.371517710732824	37.0	35.0	37.0	33.0	37.0
9	37.302210004381074	39.0	37.0	39.0	34.0	39.0
10	37.208957025469736	39.0	37.0	39.0	34.0	39.0
11	37.09588767506221	39.0	37.0	39.0	34.0	39.0
12	36.94180872092495	39.0	37.0	39.0	33.0	39.0
13	36.83032133138903	39.0	35.0	39.0	33.0	39.0
14	37.77873091433976	40.0	37.0	41.0	33.0	41.0
15	37.84311823484772	40.0	37.0	41.0	33.0	41.0
16	38.0178447203695	40.0	37.0	41.0	33.0	41.0
17	37.97875095363157	40.0	37.0	41.0	33.0	41.0
18	37.93493584904899	40.0	37.0	41.0	33.0	41.0
19	37.93660623833913	40.0	37.0	41.0	33.0	41.0
20	37.81138218325395	40.0	36.0	41.0	33.0	41.0
21	37.80442491771618	39.0	36.0	41.0	33.0	41.0
22	37.84809208121831	39.0	36.0	41.0	33.0	41.0
23	37.75333144820532	39.0	36.0	41.0	33.0	41.0
24	37.72719832880376	39.0	36.0	41.0	33.0	41.0
25	37.67717137999104	39.0	36.0	41.0	33.0	41.0
26	37.66491614262462	39.0	36.0	41.0	33.0	41.0
27	37.59805988722182	39.0	36.0	41.0	33.0	41.0
28	37.55258784253824	39.0	36.0	41.0	33.0	41.0
29	37.57166585355111	39.0	36.0	41.0	33.0	41.0
30	37.43858209233743	39.0	36.0	41.0	33.0	41.0
31	32.93336767359625	38.0	33.0	40.0	9.0	41.0
32	33.740848583312854	38.0	32.0	40.0	16.0	41.0
33	35.68685479616577	38.0	34.0	40.0	28.0	41.0
34	36.52323935824504	38.0	35.0	40.0	30.0	41.0
35	36.722590300377554	39.0	35.0	40.0	31.0	41.0
36	36.909072385889516	39.0	35.0	40.0	31.0	41.0
37	36.98064930667749	39.0	35.0	41.0	32.0	41.0
38	36.9602398259406	39.0	35.0	41.0	31.0	41.0
39	36.97780850170317	39.0	35.0	41.0	32.0	41.0
40	36.9835768589217	39.0	35.0	41.0	32.0	41.0
41	36.88235120069317	39.0	35.0	41.0	31.0	41.0
42	36.86372104767838	39.0	35.0	41.0	31.0	41.0
43	36.86871237799484	39.0	35.0	41.0	32.0	41.0
44	36.83331175859244	39.0	35.0	41.0	31.0	41.0
45	36.808636195063	39.0	35.0	40.0	31.0	41.0
46	36.76162858921032	39.0	35.0	40.0	31.0	41.0
47	36.60866659023044	39.0	35.0	40.0	31.0	41.0
48	36.6529070254193	39.0	35.0	40.0	31.0	41.0
49	36.707285123145816	39.0	35.0	40.0	31.0	41.0
50	36.52144792625272	39.0	35.0	40.0	31.0	41.0
51	35.97310565649359	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	13.0
11	14.0
12	15.0
13	30.0
14	44.0
15	74.0
16	148.0
17	296.0
18	605.0
19	1130.0
20	2056.0
21	3597.0
22	5962.0
23	9413.0
24	12719.0
25	15694.0
26	19130.0
27	23075.0
28	29184.0
29	38351.0
30	52258.0
31	75040.0
32	106743.0
33	131611.0
34	227846.0
35	277655.0
36	358273.0
37	351213.0
38	543541.0
39	688072.0
40	347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.415389974369205	20.116490151663143	23.27509946515937	11.193020408808277
2	29.504057788475862	28.489383714444127	23.653156171647964	18.353402325432047
3	28.752853329531696	28.31837727463614	25.92576652812881	17.00300286770335
4	26.902110413135556	24.245424838029734	29.9345663325776	18.917898416257113
5	23.976844531072167	36.74513483988908	21.332666701858713	17.945353927180037
6	24.24075124480651	33.20796447531183	26.015943341256026	16.535340938625634
7	72.00255400101608	5.456067046897658	15.622880701993877	6.918498250092379
8	68.84656727940052	8.548876202567653	13.1607376476763	9.443818870355534
9	63.508886719833555	6.357364456550209	15.705458723261751	14.428290100354488
10	39.90377777635814	20.24849394298956	21.82847105919425	18.019257221458044
11	28.753862018716564	22.033470324532296	26.74324186651881	22.469425790232325
12	24.058481109100832	19.208165540689347	32.869179401087436	23.86417394912239
13	23.61314483398153	21.388850689455868	36.31761874036912	18.680385736193482
14	19.00599060506893	26.36518516003022	31.665241613001598	22.96358262189925
15	15.90491019808302	23.638900031168497	39.08344448527768	21.372745285470806
16	19.081608670961216	25.846685296035144	29.344853012130834	25.726853020872802
17	19.64933256717786	26.08117190854417	29.152092508902523	25.11740301537545
18	20.06803944781664	24.182280895056984	32.382890345062485	23.36678931206389
19	19.298813075436165	26.58652519016313	28.610056563927188	25.504605170473514
20	20.106672243597092	28.315519321945683	31.524899324413607	20.05290911004362
21	19.842294808243143	29.38012351062839	30.80405640993397	19.973525271194493
22	18.248565896151415	24.2372544556323	30.139901827643932	27.374277820572352
23	18.177318816726892	27.224319361755278	30.1758784085709	24.42248341294693
24	21.96356816402093	23.510930996581553	28.19242561841893	26.333075220978586
25	18.741545923769323	28.99621640686756	24.978809121374564	27.28342854798856
26	18.017676941735086	24.2395744407575	28.60249139504068	29.140257222466737
27	21.739101197414932	24.254267679883746	28.515071665685436	25.49155945701589
28	17.728183145677917	24.604619056761294	29.330395133814392	28.3368026637464
29	19.183688016469876	23.712836948419334	26.083828123397655	31.019646911713135
30	21.42365046633382	24.120481870997395	28.731401872866837	25.724465789801947
31	25.290359587607515	24.86240638944077	23.213199572181296	26.63403445077042
32	20.897551810479406	27.135083991867276	25.940190783472428	26.027173414180893
33	20.18888041216385	21.508212242998603	27.761614467561735	30.54129287727581
34	20.15566091500886	21.139334608092312	27.930838889809785	30.774165587089048
35	16.517655254917614	22.81759167387599	31.28957213758386	29.375180933622534
36	20.186762164875628	21.941007149252712	30.544924158341342	27.32730652753032
37	18.937130756715266	24.103367777827465	33.24585756569004	23.713643899767227
38	20.875764124086256	24.38859145633536	28.05278941226035	26.68285500731804
39	20.74977884489622	22.62109902066367	28.505589987347673	28.12353214709244
40	19.5801364890959	19.05101176568688	32.15314457172234	29.215707173494877
41	17.864457054553608	21.303347469551877	29.82740991817177	31.00478555772274
42	19.82904735694854	19.189134938068165	36.0336391118559	24.948178593127395
43	21.687927032769284	19.323223353709977	33.024954634203915	25.963894979316827
44	20.589061034773888	19.64230536585661	29.291863206952424	30.47677039241708
45	20.32044710484349	17.897273076034654	28.1725208185042	33.60975900061766
46	23.99634585531295	19.6558890468795	29.546826209914272	26.800938887893277
47	15.978611754524055	19.84397595688459	37.15526786245648	27.02214442613487
48	17.938057742076158	20.214501117459502	32.16101234736431	29.686428793100028
49	20.926198583329665	16.8376114643578	36.50163727066191	25.73455268165063
50	20.25901793348502	16.823523438742473	33.24908537108162	29.668373256690888
51	18.130246654766378	16.695991502802308	29.252826935498028	35.9209349069333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2756.0
1	3363.5
2	3971.0
3	4983.5
4	5996.0
5	5045.0
6	4094.0
7	4194.5
8	4295.0
9	4805.0
10	5315.0
11	5664.5
12	6014.0
13	6082.0
14	6150.0
15	6459.5
16	6769.0
17	6723.0
18	6677.0
19	6811.0
20	6945.0
21	7418.5
22	7892.0
23	8472.0
24	9052.0
25	9648.0
26	12256.5
27	14269.0
28	16704.0
29	19139.0
30	22467.0
31	25795.0
32	30263.0
33	34731.0
34	42010.0
35	49289.0
36	53284.5
37	57280.0
38	62916.5
39	68553.0
40	79506.0
41	90459.0
42	109303.0
43	128147.0
44	161311.0
45	194475.0
46	300157.0
47	405839.0
48	420455.5
49	435072.0
50	424471.5
51	413871.0
52	341960.5
53	270050.0
54	225797.0
55	181544.0
56	155717.0
57	129890.0
58	117424.5
59	104959.0
60	92924.0
61	80889.0
62	69915.0
63	58941.0
64	50941.0
65	42941.0
66	36392.5
67	29844.0
68	24574.5
69	19305.0
70	15828.5
71	12352.0
72	10754.0
73	9156.0
74	7385.0
75	4170.5
76	2727.0
77	2160.0
78	1593.0
79	1210.5
80	828.0
81	558.5
82	289.0
83	193.5
84	98.0
85	69.5
86	41.0
87	23.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2974157.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.606514860716313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.37190307516798	14.48776741974394
2	14.130630597434646	6.671498823792672
3	6.933871505194741	4.910536221890312
4	4.071347839357135	3.8444133309171784
5	2.557053748853454	3.0181563660979713
6	1.7499120083528719	2.47855942980768
7	1.2744046478019724	2.1058976579832236
8	0.9443983170694559	1.783516232506847
9	0.6950377551761019	1.47666771866912
>10	5.224471295590433	27.0707831729102
>50	0.8888731652871666	13.744016542858429
>100	0.13627743360540867	5.556924254705073
>500	0.010987789766948353	1.8066055371545542
>1k	0.008633263388316563	3.6927306206681214
>5k	0.0012557474019369546	1.9328882090984107
>10k+	9.418105514527159E-4	5.419038461196403
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	32482	1.0921414034296104	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	27023	0.9085935947564301	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	25306	0.8508629504091412	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	24928	0.8381534666798021	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	19533	0.6567575282676738	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	16972	0.5706490948527599	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTT	9852	0.33125352831071125	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	7428	0.2497514421733621	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	6512	0.21895279906205353	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	6370	0.21417833692034416	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	5621	0.18899473027146851	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA	5569	0.18724633568436366	No Hit
ACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	5480	0.18425389110258805	TruSeq Adapter, Index 16 (95% over 22bp)
TGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCTT	5331	0.17924406815107607	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	4729	0.15900303850805456	No Hit
GCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	4239	0.14252778182187423	No Hit
ACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	3837	0.1290113467446406	TruSeq Adapter, Index 16 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	3716	0.12494296703233891	TruSeq Adapter, Index 16 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	3661	0.12309370352674724	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	3189	0.10722366035148784	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	3012	0.10127239416076554	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6811486414469715E-4	0.0	0.0	0.6902123862324686	0.0
2	1.6811486414469715E-4	0.0	0.0	2.647136650822401	0.0
3	1.6811486414469715E-4	0.0	0.0	3.669073286985186	0.0
4	1.6811486414469715E-4	0.0	0.0	5.111061722699911	0.0
5	1.6811486414469715E-4	0.0	0.0	8.915904573968355	0.0
6	1.6811486414469715E-4	0.0	0.0	10.955305990907675	0.0
7	1.6811486414469715E-4	0.0	0.0	12.983040236275354	0.0
8	1.6811486414469715E-4	0.0	0.0	16.110178447203694	0.0
9	1.6811486414469715E-4	0.0	0.0	17.1235412252951	0.0
10	2.0173783697363657E-4	0.0	0.0	20.241971086260744	0.0
11	2.3536080980257599E-4	0.0	0.0	23.415139146991905	0.0
12	2.3536080980257599E-4	0.0	0.0	27.20283428211759	0.0
13	2.3536080980257599E-4	0.0	0.0	28.2847543018072	0.0
14	2.3536080980257599E-4	0.0	0.0	28.780760396979716	0.0
15	2.3536080980257599E-4	0.0	0.0	29.766485091405734	0.0
16	2.3536080980257599E-4	0.0	0.0	31.263480710668603	0.0
17	2.3536080980257599E-4	0.0	0.0	32.88615900236605	0.0
18	2.3536080980257599E-4	0.0	0.0	34.54894950064842	0.0
19	2.689837826315154E-4	0.0	0.0	36.12321071147219	0.0
20	2.689837826315154E-4	0.0	0.0	37.191547050138915	0.0
21	2.689837826315154E-4	0.0	0.0	38.354935532993046	0.0
22	2.689837826315154E-4	0.0	0.0	39.5189292293581	0.0
23	2.689837826315154E-4	0.0	0.0	40.54627916414634	0.0
24	2.689837826315154E-4	0.0	0.0	41.38574392676647	0.0
25	2.689837826315154E-4	0.0	0.0	42.18109534903504	0.0
26	3.0260675546045485E-4	0.0	0.0	42.87725900145823	0.0
27	3.0260675546045485E-4	0.0	0.0	43.60845779156917	0.0
28	3.362297282893943E-4	0.0	0.0	44.276815245462835	0.0
29	3.362297282893943E-4	0.0	0.0	45.00764418287266	0.0
30	3.362297282893943E-4	0.0	0.0	45.800541128124706	0.0
31	3.362297282893943E-4	0.0	0.0	46.464964694197384	0.0
32	3.362297282893943E-4	0.0	0.0	47.19693681268339	0.0
33	3.362297282893943E-4	0.0	0.0	47.90291837317263	0.0
34	3.698527011183337E-4	0.0	0.0	48.649045763219625	0.0
35	3.698527011183337E-4	0.0	0.0	49.391508249228266	0.0
36	4.0347567394727313E-4	0.0	0.0	50.069414627405344	0.0
37	4.0347567394727313E-4	0.0	0.0	50.775093581139124	0.0
38	4.7072161960515197E-4	0.0	0.0	51.54895992377	0.0
39	5.043445924340914E-4	0.0	0.0	52.56117952078522	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCG	20	7.035096E-4	45.0	13
CCCGTTA	20	7.035096E-4	45.0	1
CGCTATA	20	7.035096E-4	45.0	27
TCGCGAC	20	7.035096E-4	45.0	33
TATCGCG	100	0.0	42.750004	1
TCTAACG	470	0.0	42.606384	28
TAATACG	1190	0.0	42.35294	4
CGCCTAA	575	0.0	41.47826	26
ACGTAAG	170	0.0	41.02941	1
GCCGATA	50	1.0822987E-9	40.500004	9
ACGCCTA	600	0.0	40.5	25
AATACGG	1260	0.0	40.0	5
ACGATAG	85	0.0	39.705883	1
CTTACGC	350	0.0	39.214287	29
TTACGGG	790	0.0	39.018986	3
TACGGGA	1060	0.0	38.84434	4
ATAATAC	1785	0.0	38.697475	3
GACGCGT	390	0.0	38.653847	26
CGTACTA	35	6.2516356E-6	38.571426	44
TACGGCT	4765	0.0	38.24764	7
>>END_MODULE