FastQCFastQC Report
Sat 14 Jan 2017
SRR2935111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161870
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC127080.5878244297760735TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT122880.5683968046182241TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC107680.49808730404695933TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC44130.20412883290854678No Hit
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC43010.19894813286645358TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC32940.15236808873799074TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG28910.13372681983653043No Hit
TGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCTT25730.11901733221701583TruSeq Adapter, Index 20 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG25310.11707456970123087No Hit
ACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG24730.11439170717943263TruSeq Adapter, Index 27 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG23620.10925726338771526TruSeq Adapter, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT207.034666E-445.00000413
CGATTAT302.1663946E-644.99999610
AAATGCG302.1663946E-644.9999961
GTTAGCG700.041.7857131
ACGTTAG650.041.5384641
ATACGCA2550.041.4705927
TATACGC2550.041.4705926
TGTTACG556.184564E-1140.9090921
GCCGTAT2000.040.527
CCGAATT3900.040.38461728
GCCGAAT4000.039.37527
CTTACGG2800.039.3752
CCGCATT2500.038.69999728
CGTTATT3550.038.6619721
GTCGACT356.2508443E-638.57142616
TAACGGG5450.038.3944973
TAGCCGT659.094947E-1238.07692744
TACGGGT2250.038.04
ACGCATC2800.037.76785729
GCCATAC2400.037.49999613
Produced by FastQC (version 0.11.5)