##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935111.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2161870
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.842572865158406	33.0	31.0	34.0	30.0	34.0
2	32.16440997839833	34.0	31.0	34.0	30.0	34.0
3	32.22284087387308	34.0	31.0	34.0	30.0	34.0
4	35.78372566343027	37.0	35.0	37.0	35.0	37.0
5	30.296255556532078	37.0	35.0	37.0	0.0	37.0
6	32.765408650843945	37.0	35.0	37.0	17.0	37.0
7	34.96992927419318	37.0	35.0	37.0	32.0	37.0
8	35.52536368976858	37.0	35.0	37.0	33.0	37.0
9	37.33544061391296	39.0	37.0	39.0	34.0	39.0
10	37.2868313080805	39.0	37.0	39.0	34.0	39.0
11	37.22287880399839	39.0	37.0	39.0	34.0	39.0
12	37.12703030246962	39.0	37.0	39.0	34.0	39.0
13	37.09759883804299	39.0	37.0	39.0	33.0	39.0
14	38.206705768616985	40.0	38.0	41.0	33.0	41.0
15	38.25421648850301	40.0	37.0	41.0	34.0	41.0
16	38.334364230966706	40.0	38.0	41.0	34.0	41.0
17	38.3419197269031	40.0	37.0	41.0	34.0	41.0
18	38.27447117541758	40.0	37.0	41.0	34.0	41.0
19	38.286482998515176	40.0	37.0	41.0	34.0	41.0
20	38.218141238834896	40.0	37.0	41.0	34.0	41.0
21	38.143693654105014	40.0	37.0	41.0	34.0	41.0
22	38.167151123795605	40.0	37.0	41.0	34.0	41.0
23	38.11604675581788	40.0	37.0	41.0	34.0	41.0
24	38.08608334451193	40.0	37.0	41.0	34.0	41.0
25	38.01871481633956	40.0	37.0	41.0	34.0	41.0
26	37.95996706554973	40.0	37.0	41.0	34.0	41.0
27	37.89047676317262	40.0	36.0	41.0	33.0	41.0
28	37.82403613538279	40.0	36.0	41.0	33.0	41.0
29	37.81384125779996	40.0	36.0	41.0	33.0	41.0
30	37.6871749920208	40.0	36.0	41.0	33.0	41.0
31	33.123711879067656	38.0	33.0	41.0	9.0	41.0
32	33.95142723660535	38.0	33.0	41.0	16.0	41.0
33	35.888072825840595	38.0	34.0	40.0	29.0	41.0
34	36.69430308020371	38.0	35.0	40.0	31.0	41.0
35	36.86041066299084	39.0	35.0	40.0	31.0	41.0
36	37.09559686752672	39.0	35.0	41.0	32.0	41.0
37	37.19929227936925	39.0	35.0	41.0	32.0	41.0
38	37.11135128384223	39.0	35.0	41.0	32.0	41.0
39	37.14164542733837	39.0	35.0	41.0	32.0	41.0
40	37.139799802948374	39.0	35.0	41.0	32.0	41.0
41	37.07175778377053	39.0	35.0	41.0	32.0	41.0
42	36.99920485505604	39.0	35.0	41.0	32.0	41.0
43	36.97933872064463	39.0	35.0	41.0	32.0	41.0
44	36.885962615698446	39.0	35.0	41.0	32.0	41.0
45	36.82796236591469	39.0	35.0	41.0	31.0	41.0
46	36.77136229282982	39.0	35.0	41.0	31.0	41.0
47	36.66529902353056	39.0	35.0	41.0	31.0	41.0
48	36.66900276149815	39.0	35.0	41.0	31.0	41.0
49	36.65723840934006	39.0	35.0	41.0	31.0	41.0
50	36.44569284924626	39.0	35.0	40.0	31.0	41.0
51	35.81058204239848	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	18.0
11	18.0
12	22.0
13	21.0
14	40.0
15	86.0
16	110.0
17	222.0
18	430.0
19	781.0
20	1241.0
21	2021.0
22	3487.0
23	5506.0
24	7948.0
25	10392.0
26	13091.0
27	16071.0
28	20046.0
29	25961.0
30	34824.0
31	47786.0
32	68772.0
33	86461.0
34	147348.0
35	203251.0
36	263449.0
37	265268.0
38	397782.0
39	539018.0
40	391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.687904453089224	21.370757723637407	24.202657884146596	11.738679939126774
2	32.41628775088234	23.377723914944006	24.596714881098308	19.609273453075346
3	31.342448898407397	24.46641102378959	26.438222464810558	17.752917612992455
4	28.829485584239574	25.010939603213885	26.24038448195312	19.919190330593423
5	21.7541295267523	38.40980262457965	21.88767132158733	17.94839652708072
6	26.5893416347884	31.480755087031138	25.42127880029789	16.508624477882574
7	75.73244459657612	6.416343258382789	11.109502421514707	6.741709723526392
8	74.40502897954087	5.713895840175404	11.577153112814369	8.303922067469367
9	67.442584429221	8.247813235763482	14.448417342393391	9.861184992622128
10	36.80323053652625	24.400727148255907	21.850666321286663	16.94537599393118
11	26.3724923330265	24.721144194609295	26.463200840013506	22.443162632350695
12	23.28535018294347	22.2946338123939	31.94447399704885	22.47554200761378
13	23.600540273004388	23.682737629922244	33.280354507902885	19.436367589170487
14	20.03862396906382	27.211441946092968	30.662528274133045	22.087405810710173
15	17.213893527362885	26.201205437884795	35.410223556458064	21.174677478294253
16	19.633928034525667	27.833496001147157	30.27147793345576	22.26109803087142
17	20.513906941675494	26.96554371909504	27.515623048564436	25.004926290665026
18	20.3600123966751	25.688686183720577	32.46462553252508	21.486675887079244
19	21.179626897084468	26.43530832103688	29.144028086795227	23.241036695083423
20	22.299444462432984	28.12171869723896	29.064559848649544	20.5142769916785
21	21.282454541669942	27.693015768755753	31.2341167600272	19.790412929547106
22	20.3919754656848	25.171171254515766	28.35873572416473	26.078117555634705
23	19.93362228071068	26.47434859635408	29.292973213005407	24.29905590992983
24	21.199517084746073	24.565445655844247	29.321467063236923	24.913570196172756
25	20.292709552378266	27.40437676641056	26.324987163890523	25.977926517320654
26	19.283583194179112	26.524952934265244	27.839324288694513	26.352139582861135
27	22.935375392599923	26.460194183739077	27.274165421602593	23.330265002058404
28	19.65071905341209	25.43358296289786	27.716143893943666	27.199554089746375
29	21.063847502393763	25.060341278615272	26.69670239191071	27.179108827080263
30	24.473118180094087	24.66526664415529	26.594059772326734	24.267555403423888
31	25.05950866610851	27.15958868942166	24.310666228774163	23.470236415695673
32	21.923334890627096	29.201570862262766	24.96412827783354	23.9109659692766
33	22.554038864501567	25.191338979679628	27.895664401652276	24.358957754166532
34	19.871777673958192	23.95255033836447	31.2479011226392	24.927770865038138
35	19.297413813041487	27.987483058648298	27.166110820724647	25.54899230758556
36	22.950038623969064	25.07088770370096	27.567152511483112	24.41192116084686
37	22.688644553095237	26.22655386309075	29.18584373713498	21.898957846679032
38	20.520567841729616	29.286219800450535	26.74083085476925	23.452381503050603
39	20.542678329409263	23.996262494969635	30.758695018664394	24.70236415695671
40	21.157562665655195	23.115080925310032	33.06480038115151	22.66255602788327
41	19.36430035108494	25.569668851503558	27.941319320773218	27.12471147663828
42	22.604088127408218	23.7957416495904	27.582787124110148	26.01738309889124
43	23.457053384338558	22.504128370346045	28.20447112916133	25.83434711615407
44	21.70098109507047	22.84031879807759	28.494960381521555	26.963739725330388
45	21.69677177628627	21.615360775624808	28.711023327027064	27.976844121061863
46	23.315046695684753	22.756502472396583	30.024238275196936	23.904212556721728
47	18.640667570205423	24.146734077442215	32.536831539361756	24.675766812990606
48	19.598634515488904	23.135711212977654	30.177624001443192	27.088030270090247
49	22.1501292862198	20.782516987607952	32.56837830211807	24.498975424054176
50	20.597862036107628	21.188184303403997	31.75907894554252	26.45487471494586
51	19.539935333761974	20.489391128976305	28.545564719432715	31.425108817829013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2305.0
1	2820.5
2	3336.0
3	4267.0
4	5198.0
5	4309.5
6	3421.0
7	3478.0
8	3535.0
9	3909.5
10	4284.0
11	4553.5
12	4823.0
13	5259.0
14	5695.0
15	5714.5
16	5734.0
17	5748.5
18	5763.0
19	5923.5
20	6084.0
21	7230.0
22	8376.0
23	8695.0
24	9014.0
25	10532.5
26	14359.0
27	16667.0
28	19356.5
29	22046.0
30	25099.0
31	28152.0
32	31947.0
33	35742.0
34	41459.5
35	47177.0
36	50582.5
37	53988.0
38	61611.0
39	69234.0
40	76220.5
41	83207.0
42	96071.5
43	108936.0
44	127564.5
45	146193.0
46	181206.5
47	216220.0
48	251271.0
49	286322.0
50	278733.5
51	271145.0
52	226577.5
53	182010.0
54	154806.5
55	127603.0
56	109463.5
57	91324.0
58	81194.5
59	71065.0
60	64969.5
61	58874.0
62	55066.5
63	51259.0
64	46142.0
65	41025.0
66	34066.5
67	27108.0
68	22671.5
69	18235.0
70	14601.0
71	10967.0
72	9232.5
73	7498.0
74	6408.0
75	3928.5
76	2539.0
77	1797.0
78	1055.0
79	914.0
80	773.0
81	541.5
82	310.0
83	241.0
84	172.0
85	124.5
86	77.0
87	42.5
88	8.0
89	4.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2161870.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.471664008314526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.836595560883225	16.898609091223634
2	14.116141702046225	7.473555204606496
3	6.715638108963828	5.333223468657697
4	3.6712982997761623	3.887415002638838
5	2.2202494959125225	2.9386849335212997
6	1.4749937182431334	2.3427322874224066
7	1.0260154460790676	1.9012235309162222
8	0.7191182216912342	1.5229004757493594
9	0.5428512131801813	1.2933157429630513
>10	4.592424655353574	28.584585281583486
>50	0.9676315999213261	16.71674447222948
>100	0.1015141133344898	4.77208648189382
>500	0.009122620461321696	1.6742001937055988
>1k	0.00582294923063087	2.823131894277993
>5k	0.0	0.0
>10k+	5.82294923063087E-4	1.8375919386105766
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	12708	0.5878244297760735	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT	12288	0.5683968046182241	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	10768	0.49808730404695933	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC	4413	0.20412883290854678	No Hit
TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	4301	0.19894813286645358	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	3294	0.15236808873799074	TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	2891	0.13372681983653043	No Hit
TGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCTT	2573	0.11901733221701583	TruSeq Adapter, Index 20 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	2531	0.11707456970123087	No Hit
ACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	2473	0.11439170717943263	TruSeq Adapter, Index 27 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG	2362	0.10925726338771526	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.251250075166406E-5	0.0	0.0	0.5993884923700316	0.0
2	9.251250075166406E-5	0.0	0.0	2.1146044859311615	0.0
3	9.251250075166406E-5	0.0	0.0	3.015907524504249	0.0
4	9.251250075166406E-5	0.0	0.0	4.070735058074722	0.0
5	9.251250075166406E-5	0.0	0.0	6.749573286090283	0.0
6	9.251250075166406E-5	0.0	0.0	8.455226262448713	0.0
7	9.251250075166406E-5	0.0	0.0	9.987048249894768	0.0
8	9.251250075166406E-5	0.0	0.0	12.42082086341917	0.0
9	9.251250075166406E-5	0.0	0.0	13.286044026699107	0.0
10	9.251250075166406E-5	0.0	0.0	15.114229810303117	0.0
11	9.251250075166406E-5	0.0	0.0	17.86222113263055	0.0
12	9.251250075166406E-5	0.0	0.0	20.001480200012026	0.0
13	9.251250075166406E-5	0.0	0.0	20.85587940070402	0.0
14	9.251250075166406E-5	0.0	0.0	21.2494275789016	0.0
15	9.251250075166406E-5	0.0	0.0	21.96325403470144	0.0
16	9.251250075166406E-5	0.0	0.0	23.295942864279535	0.0
17	9.251250075166406E-5	0.0	0.0	24.82189030792786	0.0
18	1.387687511274961E-4	0.0	0.0	26.534065415589282	0.0
19	1.387687511274961E-4	0.0	0.0	27.592963499192827	0.0
20	1.387687511274961E-4	0.0	0.0	28.495700481527567	0.0
21	1.8502500150332813E-4	0.0	0.0	29.540074102513103	0.0
22	1.8502500150332813E-4	0.0	0.0	30.618584836276003	0.0
23	2.775375022549922E-4	0.0	0.0	31.578170750322638	0.0
24	2.775375022549922E-4	0.0	0.0	32.400005550750045	0.0
25	2.775375022549922E-4	0.0	0.0	33.12826395666715	0.0
26	2.775375022549922E-4	0.0	0.0	33.78977459329192	0.0
27	2.775375022549922E-4	0.0	0.0	34.43093247975133	0.0
28	2.775375022549922E-4	0.0	0.0	35.07893629126636	0.0
29	2.775375022549922E-4	0.0	0.0	35.77231748440008	0.0
30	2.775375022549922E-4	0.0	0.0	36.496089034030724	0.0
31	3.2379375263082426E-4	0.0	0.0	37.15112379560288	0.0
32	3.7005000300665625E-4	0.0	0.0	37.89381415163724	0.0
33	3.7005000300665625E-4	0.0	0.0	38.59811181985966	0.0
34	3.7005000300665625E-4	0.0	0.0	39.29491597552119	0.0
35	4.163062533824883E-4	0.0	0.0	39.9764555685587	0.0
36	4.163062533824883E-4	0.0	0.0	40.639261380194	0.0
37	4.163062533824883E-4	0.0	0.0	41.31821062321046	0.0
38	4.6256250375832034E-4	0.0	0.0	41.99234921618784	0.0
39	4.6256250375832034E-4	0.0	0.0	42.86742496079783	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCGT	20	7.034666E-4	45.000004	13
CGATTAT	30	2.1663946E-6	44.999996	10
AAATGCG	30	2.1663946E-6	44.999996	1
GTTAGCG	70	0.0	41.785713	1
ACGTTAG	65	0.0	41.538464	1
ATACGCA	255	0.0	41.47059	27
TATACGC	255	0.0	41.47059	26
TGTTACG	55	6.184564E-11	40.909092	1
GCCGTAT	200	0.0	40.5	27
CCGAATT	390	0.0	40.384617	28
GCCGAAT	400	0.0	39.375	27
CTTACGG	280	0.0	39.375	2
CCGCATT	250	0.0	38.699997	28
CGTTATT	355	0.0	38.661972	1
GTCGACT	35	6.2508443E-6	38.571426	16
TAACGGG	545	0.0	38.394497	3
TAGCCGT	65	9.094947E-12	38.076927	44
TACGGGT	225	0.0	38.0	4
ACGCATC	280	0.0	37.767857	29
GCCATAC	240	0.0	37.499996	13
>>END_MODULE