##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935111.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2161870 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.842572865158406 33.0 31.0 34.0 30.0 34.0 2 32.16440997839833 34.0 31.0 34.0 30.0 34.0 3 32.22284087387308 34.0 31.0 34.0 30.0 34.0 4 35.78372566343027 37.0 35.0 37.0 35.0 37.0 5 30.296255556532078 37.0 35.0 37.0 0.0 37.0 6 32.765408650843945 37.0 35.0 37.0 17.0 37.0 7 34.96992927419318 37.0 35.0 37.0 32.0 37.0 8 35.52536368976858 37.0 35.0 37.0 33.0 37.0 9 37.33544061391296 39.0 37.0 39.0 34.0 39.0 10 37.2868313080805 39.0 37.0 39.0 34.0 39.0 11 37.22287880399839 39.0 37.0 39.0 34.0 39.0 12 37.12703030246962 39.0 37.0 39.0 34.0 39.0 13 37.09759883804299 39.0 37.0 39.0 33.0 39.0 14 38.206705768616985 40.0 38.0 41.0 33.0 41.0 15 38.25421648850301 40.0 37.0 41.0 34.0 41.0 16 38.334364230966706 40.0 38.0 41.0 34.0 41.0 17 38.3419197269031 40.0 37.0 41.0 34.0 41.0 18 38.27447117541758 40.0 37.0 41.0 34.0 41.0 19 38.286482998515176 40.0 37.0 41.0 34.0 41.0 20 38.218141238834896 40.0 37.0 41.0 34.0 41.0 21 38.143693654105014 40.0 37.0 41.0 34.0 41.0 22 38.167151123795605 40.0 37.0 41.0 34.0 41.0 23 38.11604675581788 40.0 37.0 41.0 34.0 41.0 24 38.08608334451193 40.0 37.0 41.0 34.0 41.0 25 38.01871481633956 40.0 37.0 41.0 34.0 41.0 26 37.95996706554973 40.0 37.0 41.0 34.0 41.0 27 37.89047676317262 40.0 36.0 41.0 33.0 41.0 28 37.82403613538279 40.0 36.0 41.0 33.0 41.0 29 37.81384125779996 40.0 36.0 41.0 33.0 41.0 30 37.6871749920208 40.0 36.0 41.0 33.0 41.0 31 33.123711879067656 38.0 33.0 41.0 9.0 41.0 32 33.95142723660535 38.0 33.0 41.0 16.0 41.0 33 35.888072825840595 38.0 34.0 40.0 29.0 41.0 34 36.69430308020371 38.0 35.0 40.0 31.0 41.0 35 36.86041066299084 39.0 35.0 40.0 31.0 41.0 36 37.09559686752672 39.0 35.0 41.0 32.0 41.0 37 37.19929227936925 39.0 35.0 41.0 32.0 41.0 38 37.11135128384223 39.0 35.0 41.0 32.0 41.0 39 37.14164542733837 39.0 35.0 41.0 32.0 41.0 40 37.139799802948374 39.0 35.0 41.0 32.0 41.0 41 37.07175778377053 39.0 35.0 41.0 32.0 41.0 42 36.99920485505604 39.0 35.0 41.0 32.0 41.0 43 36.97933872064463 39.0 35.0 41.0 32.0 41.0 44 36.885962615698446 39.0 35.0 41.0 32.0 41.0 45 36.82796236591469 39.0 35.0 41.0 31.0 41.0 46 36.77136229282982 39.0 35.0 41.0 31.0 41.0 47 36.66529902353056 39.0 35.0 41.0 31.0 41.0 48 36.66900276149815 39.0 35.0 41.0 31.0 41.0 49 36.65723840934006 39.0 35.0 41.0 31.0 41.0 50 36.44569284924626 39.0 35.0 40.0 31.0 41.0 51 35.81058204239848 38.0 35.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 6.0 10 18.0 11 18.0 12 22.0 13 21.0 14 40.0 15 86.0 16 110.0 17 222.0 18 430.0 19 781.0 20 1241.0 21 2021.0 22 3487.0 23 5506.0 24 7948.0 25 10392.0 26 13091.0 27 16071.0 28 20046.0 29 25961.0 30 34824.0 31 47786.0 32 68772.0 33 86461.0 34 147348.0 35 203251.0 36 263449.0 37 265268.0 38 397782.0 39 539018.0 40 391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.687904453089224 21.370757723637407 24.202657884146596 11.738679939126774 2 32.41628775088234 23.377723914944006 24.596714881098308 19.609273453075346 3 31.342448898407397 24.46641102378959 26.438222464810558 17.752917612992455 4 28.829485584239574 25.010939603213885 26.24038448195312 19.919190330593423 5 21.7541295267523 38.40980262457965 21.88767132158733 17.94839652708072 6 26.5893416347884 31.480755087031138 25.42127880029789 16.508624477882574 7 75.73244459657612 6.416343258382789 11.109502421514707 6.741709723526392 8 74.40502897954087 5.713895840175404 11.577153112814369 8.303922067469367 9 67.442584429221 8.247813235763482 14.448417342393391 9.861184992622128 10 36.80323053652625 24.400727148255907 21.850666321286663 16.94537599393118 11 26.3724923330265 24.721144194609295 26.463200840013506 22.443162632350695 12 23.28535018294347 22.2946338123939 31.94447399704885 22.47554200761378 13 23.600540273004388 23.682737629922244 33.280354507902885 19.436367589170487 14 20.03862396906382 27.211441946092968 30.662528274133045 22.087405810710173 15 17.213893527362885 26.201205437884795 35.410223556458064 21.174677478294253 16 19.633928034525667 27.833496001147157 30.27147793345576 22.26109803087142 17 20.513906941675494 26.96554371909504 27.515623048564436 25.004926290665026 18 20.3600123966751 25.688686183720577 32.46462553252508 21.486675887079244 19 21.179626897084468 26.43530832103688 29.144028086795227 23.241036695083423 20 22.299444462432984 28.12171869723896 29.064559848649544 20.5142769916785 21 21.282454541669942 27.693015768755753 31.2341167600272 19.790412929547106 22 20.3919754656848 25.171171254515766 28.35873572416473 26.078117555634705 23 19.93362228071068 26.47434859635408 29.292973213005407 24.29905590992983 24 21.199517084746073 24.565445655844247 29.321467063236923 24.913570196172756 25 20.292709552378266 27.40437676641056 26.324987163890523 25.977926517320654 26 19.283583194179112 26.524952934265244 27.839324288694513 26.352139582861135 27 22.935375392599923 26.460194183739077 27.274165421602593 23.330265002058404 28 19.65071905341209 25.43358296289786 27.716143893943666 27.199554089746375 29 21.063847502393763 25.060341278615272 26.69670239191071 27.179108827080263 30 24.473118180094087 24.66526664415529 26.594059772326734 24.267555403423888 31 25.05950866610851 27.15958868942166 24.310666228774163 23.470236415695673 32 21.923334890627096 29.201570862262766 24.96412827783354 23.9109659692766 33 22.554038864501567 25.191338979679628 27.895664401652276 24.358957754166532 34 19.871777673958192 23.95255033836447 31.2479011226392 24.927770865038138 35 19.297413813041487 27.987483058648298 27.166110820724647 25.54899230758556 36 22.950038623969064 25.07088770370096 27.567152511483112 24.41192116084686 37 22.688644553095237 26.22655386309075 29.18584373713498 21.898957846679032 38 20.520567841729616 29.286219800450535 26.74083085476925 23.452381503050603 39 20.542678329409263 23.996262494969635 30.758695018664394 24.70236415695671 40 21.157562665655195 23.115080925310032 33.06480038115151 22.66255602788327 41 19.36430035108494 25.569668851503558 27.941319320773218 27.12471147663828 42 22.604088127408218 23.7957416495904 27.582787124110148 26.01738309889124 43 23.457053384338558 22.504128370346045 28.20447112916133 25.83434711615407 44 21.70098109507047 22.84031879807759 28.494960381521555 26.963739725330388 45 21.69677177628627 21.615360775624808 28.711023327027064 27.976844121061863 46 23.315046695684753 22.756502472396583 30.024238275196936 23.904212556721728 47 18.640667570205423 24.146734077442215 32.536831539361756 24.675766812990606 48 19.598634515488904 23.135711212977654 30.177624001443192 27.088030270090247 49 22.1501292862198 20.782516987607952 32.56837830211807 24.498975424054176 50 20.597862036107628 21.188184303403997 31.75907894554252 26.45487471494586 51 19.539935333761974 20.489391128976305 28.545564719432715 31.425108817829013 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2305.0 1 2820.5 2 3336.0 3 4267.0 4 5198.0 5 4309.5 6 3421.0 7 3478.0 8 3535.0 9 3909.5 10 4284.0 11 4553.5 12 4823.0 13 5259.0 14 5695.0 15 5714.5 16 5734.0 17 5748.5 18 5763.0 19 5923.5 20 6084.0 21 7230.0 22 8376.0 23 8695.0 24 9014.0 25 10532.5 26 14359.0 27 16667.0 28 19356.5 29 22046.0 30 25099.0 31 28152.0 32 31947.0 33 35742.0 34 41459.5 35 47177.0 36 50582.5 37 53988.0 38 61611.0 39 69234.0 40 76220.5 41 83207.0 42 96071.5 43 108936.0 44 127564.5 45 146193.0 46 181206.5 47 216220.0 48 251271.0 49 286322.0 50 278733.5 51 271145.0 52 226577.5 53 182010.0 54 154806.5 55 127603.0 56 109463.5 57 91324.0 58 81194.5 59 71065.0 60 64969.5 61 58874.0 62 55066.5 63 51259.0 64 46142.0 65 41025.0 66 34066.5 67 27108.0 68 22671.5 69 18235.0 70 14601.0 71 10967.0 72 9232.5 73 7498.0 74 6408.0 75 3928.5 76 2539.0 77 1797.0 78 1055.0 79 914.0 80 773.0 81 541.5 82 310.0 83 241.0 84 172.0 85 124.5 86 77.0 87 42.5 88 8.0 89 4.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2161870.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.471664008314526 #Duplication Level Percentage of deduplicated Percentage of total 1 63.836595560883225 16.898609091223634 2 14.116141702046225 7.473555204606496 3 6.715638108963828 5.333223468657697 4 3.6712982997761623 3.887415002638838 5 2.2202494959125225 2.9386849335212997 6 1.4749937182431334 2.3427322874224066 7 1.0260154460790676 1.9012235309162222 8 0.7191182216912342 1.5229004757493594 9 0.5428512131801813 1.2933157429630513 >10 4.592424655353574 28.584585281583486 >50 0.9676315999213261 16.71674447222948 >100 0.1015141133344898 4.77208648189382 >500 0.009122620461321696 1.6742001937055988 >1k 0.00582294923063087 2.823131894277993 >5k 0.0 0.0 >10k+ 5.82294923063087E-4 1.8375919386105766 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 12708 0.5878244297760735 TruSeq Adapter, Index 20 (95% over 21bp) CTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCT 12288 0.5683968046182241 TruSeq Adapter, Index 27 (95% over 23bp) CCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 10768 0.49808730404695933 TruSeq Adapter, Index 20 (95% over 21bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCC 4413 0.20412883290854678 No Hit TCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 4301 0.19894813286645358 TruSeq Adapter, Index 20 (95% over 21bp) ACTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC 3294 0.15236808873799074 TruSeq Adapter, Index 27 (95% over 22bp) GCCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG 2891 0.13372681983653043 No Hit TGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGCTT 2573 0.11901733221701583 TruSeq Adapter, Index 20 (95% over 23bp) GGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG 2531 0.11707456970123087 No Hit ACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG 2473 0.11439170717943263 TruSeq Adapter, Index 27 (95% over 21bp) AGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTG 2362 0.10925726338771526 TruSeq Adapter, Index 27 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.251250075166406E-5 0.0 0.0 0.5993884923700316 0.0 2 9.251250075166406E-5 0.0 0.0 2.1146044859311615 0.0 3 9.251250075166406E-5 0.0 0.0 3.015907524504249 0.0 4 9.251250075166406E-5 0.0 0.0 4.070735058074722 0.0 5 9.251250075166406E-5 0.0 0.0 6.749573286090283 0.0 6 9.251250075166406E-5 0.0 0.0 8.455226262448713 0.0 7 9.251250075166406E-5 0.0 0.0 9.987048249894768 0.0 8 9.251250075166406E-5 0.0 0.0 12.42082086341917 0.0 9 9.251250075166406E-5 0.0 0.0 13.286044026699107 0.0 10 9.251250075166406E-5 0.0 0.0 15.114229810303117 0.0 11 9.251250075166406E-5 0.0 0.0 17.86222113263055 0.0 12 9.251250075166406E-5 0.0 0.0 20.001480200012026 0.0 13 9.251250075166406E-5 0.0 0.0 20.85587940070402 0.0 14 9.251250075166406E-5 0.0 0.0 21.2494275789016 0.0 15 9.251250075166406E-5 0.0 0.0 21.96325403470144 0.0 16 9.251250075166406E-5 0.0 0.0 23.295942864279535 0.0 17 9.251250075166406E-5 0.0 0.0 24.82189030792786 0.0 18 1.387687511274961E-4 0.0 0.0 26.534065415589282 0.0 19 1.387687511274961E-4 0.0 0.0 27.592963499192827 0.0 20 1.387687511274961E-4 0.0 0.0 28.495700481527567 0.0 21 1.8502500150332813E-4 0.0 0.0 29.540074102513103 0.0 22 1.8502500150332813E-4 0.0 0.0 30.618584836276003 0.0 23 2.775375022549922E-4 0.0 0.0 31.578170750322638 0.0 24 2.775375022549922E-4 0.0 0.0 32.400005550750045 0.0 25 2.775375022549922E-4 0.0 0.0 33.12826395666715 0.0 26 2.775375022549922E-4 0.0 0.0 33.78977459329192 0.0 27 2.775375022549922E-4 0.0 0.0 34.43093247975133 0.0 28 2.775375022549922E-4 0.0 0.0 35.07893629126636 0.0 29 2.775375022549922E-4 0.0 0.0 35.77231748440008 0.0 30 2.775375022549922E-4 0.0 0.0 36.496089034030724 0.0 31 3.2379375263082426E-4 0.0 0.0 37.15112379560288 0.0 32 3.7005000300665625E-4 0.0 0.0 37.89381415163724 0.0 33 3.7005000300665625E-4 0.0 0.0 38.59811181985966 0.0 34 3.7005000300665625E-4 0.0 0.0 39.29491597552119 0.0 35 4.163062533824883E-4 0.0 0.0 39.9764555685587 0.0 36 4.163062533824883E-4 0.0 0.0 40.639261380194 0.0 37 4.163062533824883E-4 0.0 0.0 41.31821062321046 0.0 38 4.6256250375832034E-4 0.0 0.0 41.99234921618784 0.0 39 4.6256250375832034E-4 0.0 0.0 42.86742496079783 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGT 20 7.034666E-4 45.000004 13 CGATTAT 30 2.1663946E-6 44.999996 10 AAATGCG 30 2.1663946E-6 44.999996 1 GTTAGCG 70 0.0 41.785713 1 ACGTTAG 65 0.0 41.538464 1 ATACGCA 255 0.0 41.47059 27 TATACGC 255 0.0 41.47059 26 TGTTACG 55 6.184564E-11 40.909092 1 GCCGTAT 200 0.0 40.5 27 CCGAATT 390 0.0 40.384617 28 GCCGAAT 400 0.0 39.375 27 CTTACGG 280 0.0 39.375 2 CCGCATT 250 0.0 38.699997 28 CGTTATT 355 0.0 38.661972 1 GTCGACT 35 6.2508443E-6 38.571426 16 TAACGGG 545 0.0 38.394497 3 TAGCCGT 65 9.094947E-12 38.076927 44 TACGGGT 225 0.0 38.0 4 ACGCATC 280 0.0 37.767857 29 GCCATAC 240 0.0 37.499996 13 >>END_MODULE