##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935110.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997001
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.850580892095394	33.0	31.0	34.0	30.0	34.0
2	32.1776437536171	34.0	31.0	34.0	30.0	34.0
3	32.23764168742058	34.0	31.0	34.0	30.0	34.0
4	35.80671433629455	37.0	35.0	37.0	35.0	37.0
5	30.2959936850615	37.0	35.0	37.0	0.0	37.0
6	32.76160705957165	37.0	35.0	37.0	17.0	37.0
7	34.96136714005302	37.0	35.0	37.0	32.0	37.0
8	35.51154111179427	37.0	35.0	37.0	33.0	37.0
9	37.344678691395494	39.0	37.0	39.0	34.0	39.0
10	37.30588835918921	39.0	37.0	39.0	34.0	39.0
11	37.20530872085384	39.0	37.0	39.0	34.0	39.0
12	37.03916846623022	39.0	37.0	39.0	33.0	39.0
13	36.99681544953315	39.0	37.0	39.0	33.0	39.0
14	38.1014482432816	40.0	37.0	41.0	33.0	41.0
15	38.2422685634217	40.0	37.0	41.0	34.0	41.0
16	38.32284521279317	40.0	37.0	41.0	34.0	41.0
17	38.321995664999335	40.0	37.0	41.0	34.0	41.0
18	38.281341743889925	40.0	37.0	41.0	34.0	41.0
19	38.24260557411677	40.0	37.0	41.0	34.0	41.0
20	38.14500787862801	40.0	37.0	41.0	34.0	41.0
21	38.07563181982766	40.0	36.0	41.0	34.0	41.0
22	38.059865536744695	40.0	36.0	41.0	34.0	41.0
23	37.993039124333876	40.0	36.0	41.0	34.0	41.0
24	37.96641628243101	40.0	36.0	41.0	34.0	41.0
25	37.88513150939668	40.0	36.0	41.0	33.0	41.0
26	37.78359801043329	40.0	36.0	41.0	33.0	41.0
27	37.744873876756394	40.0	36.0	41.0	33.0	41.0
28	37.69478766821698	40.0	36.0	41.0	33.0	41.0
29	37.66797525779813	40.0	36.0	41.0	33.0	41.0
30	37.559005457366645	39.0	36.0	41.0	33.0	41.0
31	32.96409331585425	38.0	33.0	40.0	9.0	41.0
32	33.80437833061351	38.0	33.0	40.0	16.0	41.0
33	35.74180868424405	38.0	34.0	40.0	29.0	41.0
34	36.598517955348086	38.0	35.0	40.0	31.0	41.0
35	36.73105744126636	39.0	35.0	40.0	31.0	41.0
36	36.89541033559645	39.0	35.0	41.0	31.0	41.0
37	37.034871579868025	39.0	35.0	41.0	32.0	41.0
38	36.99629187934616	39.0	35.0	41.0	32.0	41.0
39	36.984975942852614	39.0	35.0	41.0	32.0	41.0
40	36.9502237209391	39.0	35.0	41.0	32.0	41.0
41	36.86528398667604	39.0	35.0	41.0	32.0	41.0
42	36.75300626579111	39.0	35.0	41.0	31.0	41.0
43	36.73048873571842	39.0	35.0	41.0	31.0	41.0
44	36.642156828328154	39.0	35.0	41.0	31.0	41.0
45	36.623286235420025	39.0	35.0	41.0	31.0	41.0
46	36.56693824780517	39.0	35.0	41.0	31.0	41.0
47	36.44817908908818	39.0	35.0	41.0	31.0	41.0
48	36.44603465793916	39.0	35.0	41.0	31.0	41.0
49	36.43109184444148	39.0	35.0	41.0	31.0	41.0
50	36.25554738661245	39.0	35.0	40.0	31.0	41.0
51	35.597091677942146	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	7.0
11	6.0
12	7.0
13	16.0
14	21.0
15	24.0
16	60.0
17	122.0
18	293.0
19	487.0
20	885.0
21	1464.0
22	2279.0
23	3614.0
24	5021.0
25	5851.0
26	6861.0
27	8104.0
28	9799.0
29	12067.0
30	15778.0
31	22013.0
32	33616.0
33	39379.0
34	68456.0
35	99751.0
36	116287.0
37	116110.0
38	176330.0
39	252120.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.039478395708734	22.060158415086846	25.764668240051915	12.135694949152509
2	31.598162890508636	25.605089663902042	25.687837825639093	17.10890961995023
3	31.1700790671223	24.901078333923437	26.835279001726175	17.093563597228087
4	28.400573319384836	26.54861930930862	26.259953600848945	18.790853770457602
5	21.06056062130329	40.058836450515095	20.992356075871538	17.88824685231008
6	24.748019309910422	32.991040129347915	25.953233748010284	16.307706812731382
7	75.11406708719448	7.6061107260674765	10.726468679570031	6.553353507167997
8	73.65629522939295	6.715138700964192	11.331081914662072	8.297484154980788
9	67.63022303889365	8.786350264443064	14.118641806778529	9.464784889884765
10	37.96555871057301	24.031470379668626	21.446819010211623	16.55615189954674
11	27.28542900157573	25.91913147529441	25.807195780144653	20.988243742985212
12	24.964769343260436	23.544509985446354	30.811904902803505	20.6788157684897
13	23.347719811715333	25.910505606313333	31.4866283985673	19.25514618340403
14	17.74842753417499	30.073189495296393	29.903179635727543	22.27520333480107
15	16.353945482502024	27.605187958688106	34.97569210060973	21.065174458200143
16	18.304093977839543	28.05694277137134	30.34089233611601	23.298070914673104
17	17.902489566209063	27.68191807229882	27.44851810579929	26.967074255692825
18	18.797674225000776	26.485429803982143	31.32504380637532	23.39185216464176
19	19.618134786223884	29.087734114609713	28.647614194970718	22.646516904195682
20	20.981022085233615	29.81992996997997	28.698366400836107	20.500681543950307
21	20.11422255343776	29.791444542182006	30.09575717577013	19.998575728610103
22	18.06668197925579	27.63507759771555	27.50418505096785	26.79405537206081
23	18.125057046081196	28.036581708543924	28.45323124049023	25.385130004884648
24	20.441403769905946	26.30478805939011	28.868677162811274	24.38513100789267
25	17.979119379017675	29.455537155930635	26.205690866909865	26.359652598141825
26	17.14160768143663	30.77238638677394	27.628457744776586	24.45754818701285
27	20.831674190898504	28.499670511865084	27.129160351895333	23.539494945341076
28	18.815828670181876	27.47088518466882	27.888136521427764	25.82514962372154
29	20.07169501334502	27.259551394632503	26.27098668908055	26.39776690294192
30	21.017431276397915	27.044606775720386	25.22545112793267	26.712510819949024
31	25.151529436780905	28.60277973642955	23.034380105937707	23.211310720851834
32	22.070689999307923	29.421033680006335	24.348320613519945	24.15995570716579
33	21.726457646481798	26.508599289268513	24.50950400250351	27.255439061746177
34	20.171594612242114	25.007397184155284	28.987734214910514	25.833273988692085
35	19.64982983968923	26.426352631541995	27.91281051874572	26.011007010023057
36	20.315125060055106	27.917324054840464	26.811106508418746	24.95644437668568
37	22.110910620952236	25.078911656056512	30.22705092572625	22.583126797265
38	22.44691830800571	25.85734618119741	27.138087123282723	24.557648387514156
39	22.974901730289137	24.352733848812587	28.218126160354906	24.45423826054337
40	21.135485320476107	23.00659678375448	31.884220778113566	23.973697117655853
41	17.784535822932977	25.397968507554154	29.750923018131374	27.06657265138149
42	21.23939695145742	25.35764758510774	29.18352138062048	24.21943408281436
43	23.503286355780986	23.696967204646736	28.133371982575746	24.666374456996532
44	21.53347890323079	23.096366001638916	26.92244039875587	28.447714696374426
45	22.114722051432246	21.717631175896514	27.57810674211962	28.589540030551625
46	23.190247552409677	22.18774103536506	29.98131396056774	24.64069745165752
47	18.29045306875319	23.428361656608168	33.01661683388482	25.26456844075382
48	18.647724525853032	22.308001697089573	32.78261506257266	26.261658714484742
49	22.24380918374204	19.996268810161673	32.91200309728877	24.847918908807515
50	20.092356978578756	19.53097338919419	32.97529290341735	27.4013767288097
51	18.548627333372785	19.969588796801606	29.61020099277734	31.871582877048265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2178.0
1	2335.5
2	2493.0
3	2671.0
4	2849.0
5	2481.0
6	2113.0
7	2139.5
8	2166.0
9	2456.5
10	2747.0
11	2957.5
12	3168.0
13	3470.0
14	3772.0
15	3748.5
16	3725.0
17	3746.0
18	3767.0
19	3790.5
20	3814.0
21	3691.5
22	3569.0
23	3540.0
24	3511.0
25	4627.5
26	6663.0
27	7582.0
28	8921.0
29	10260.0
30	11457.0
31	12654.0
32	13070.0
33	13486.0
34	16492.0
35	19498.0
36	21098.0
37	22698.0
38	24982.5
39	27267.0
40	32674.5
41	38082.0
42	46374.0
43	54666.0
44	66162.5
45	77659.0
46	92186.5
47	106714.0
48	122335.5
49	137957.0
50	132396.0
51	126835.0
52	106087.5
53	85340.0
54	71427.5
55	57515.0
56	49132.5
57	40750.0
58	35247.5
59	29745.0
60	26679.0
61	23613.0
62	20784.5
63	17956.0
64	15597.0
65	13238.0
66	11172.5
67	9107.0
68	8001.0
69	6895.0
70	5432.5
71	3970.0
72	3554.5
73	3139.0
74	2753.0
75	1743.0
76	1119.0
77	921.5
78	724.0
79	484.5
80	245.0
81	232.0
82	219.0
83	150.5
84	82.0
85	42.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	997001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.31910689555212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.37254498124642	18.512920893582063
2	13.833866027909547	7.835254616462349
3	6.337656711920029	5.384303336765299
4	3.535072440030463	4.0044037725097175
5	2.1665634608761706	3.067757112227482
6	1.4074510315808288	2.3914653728155555
7	0.947494578996941	1.8782540185899366
8	0.7220803272130825	1.6358935978818028
9	0.49448010735122216	1.2602911516023012
>10	4.219320838921046	27.634777530591382
>50	0.8380427053243378	15.57699279048258
>100	0.11224499237266591	5.678747727403331
>500	0.008388415800092443	1.6163746992463504
>1k	0.003994483714329735	2.0725885114137887
>5k	7.98896742865947E-4	1.4499748684260425
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	6923	0.6943824529764765	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	5895	0.5912732284120076	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	4926	0.49408175117176417	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2182	0.2188563501942325	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC	1892	0.1897691175836333	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1808	0.1813438502067701	No Hit
ACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1715	0.17201587561095727	TruSeq Adapter, Index 22 (95% over 21bp)
TGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCTT	1312	0.13159465236243495	TruSeq Adapter, Index 20 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	1271	0.12748231947610886	No Hit
GCCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	1118	0.11213629675396514	TruSeq Adapter, Index 20 (95% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	1091	0.10942817509711625	No Hit
GGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	1007	0.10100290772025304	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8216641708483743	0.0
2	0.0	0.0	0.0	2.592775734427548	0.0
3	0.0	0.0	0.0	3.5819422447921316	0.0
4	2.0060160421102888E-4	0.0	0.0	4.760276067927715	0.0
5	2.0060160421102888E-4	0.0	0.0	7.552851000149448	0.0
6	2.0060160421102888E-4	0.0	0.0	9.394173125202482	0.0
7	2.0060160421102888E-4	0.0	0.0	11.004803405412833	0.0
8	2.0060160421102888E-4	0.0	0.0	13.630578103733095	0.0
9	2.0060160421102888E-4	0.0	0.0	14.54100848444485	0.0
10	2.0060160421102888E-4	0.0	0.0	16.418338597453765	0.0
11	2.0060160421102888E-4	0.0	0.0	19.69606850945987	0.0
12	2.0060160421102888E-4	0.0	0.0	22.19686840835666	0.0
13	2.0060160421102888E-4	0.0	0.0	23.142805273013767	0.0
14	2.0060160421102888E-4	0.0	0.0	23.548923220738995	0.0
15	2.0060160421102888E-4	0.0	0.0	24.275000727180814	0.0
16	2.0060160421102888E-4	0.0	0.0	25.756443574279263	0.0
17	2.0060160421102888E-4	0.0	0.0	27.396060786298108	0.0
18	4.0120320842205776E-4	0.0	0.0	29.365467035639885	0.0
19	5.015040105275721E-4	0.0	0.0	30.500571213067992	0.0
20	5.015040105275721E-4	0.0	0.0	31.453027629861957	0.0
21	5.015040105275721E-4	0.0	0.0	32.54309674714469	0.0
22	5.015040105275721E-4	0.0	0.0	33.57569350482096	0.0
23	5.015040105275721E-4	0.0	0.0	34.54760827722339	0.0
24	5.015040105275721E-4	0.0	0.0	35.36114808310122	0.0
25	5.015040105275721E-4	0.0	0.0	36.12042515503997	0.0
26	5.015040105275721E-4	0.0	0.0	36.76044457327525	0.0
27	5.015040105275721E-4	0.0	0.0	37.33135673885984	0.0
28	5.015040105275721E-4	0.0	0.0	37.94138621726558	0.0
29	5.015040105275721E-4	0.0	0.0	38.608988356079884	0.0
30	5.015040105275721E-4	0.0	0.0	39.359639559037554	0.0
31	5.015040105275721E-4	0.0	0.0	40.01129387031708	0.0
32	5.015040105275721E-4	0.0	0.0	40.70497421767882	0.0
33	5.015040105275721E-4	0.0	0.0	41.37668868937945	0.0
34	5.015040105275721E-4	0.0	0.0	42.03957669049479	0.0
35	5.015040105275721E-4	0.0	0.0	42.78300623570087	0.0
36	5.015040105275721E-4	0.0	0.0	43.417609410622454	0.0
37	6.018048126330866E-4	0.0	0.0	44.07488056681989	0.0
38	6.018048126330866E-4	0.0	0.0	44.7225228460152	0.0
39	6.018048126330866E-4	0.0	0.0	45.4081791292085	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	20	7.032835E-4	45.000004	34
ATATGCG	40	6.8139343E-9	45.000004	1
CATAGGT	20	7.032835E-4	45.000004	19
CTAAACG	20	7.032835E-4	45.000004	1
AACGCAC	75	0.0	45.000004	31
TGCCTCG	20	7.032835E-4	45.000004	29
GTTCACG	20	7.032835E-4	45.000004	1
AACCTCG	20	7.032835E-4	45.000004	17
ACCGTGC	20	7.032835E-4	45.000004	24
GCGCTAC	20	7.032835E-4	45.000004	37
CCGTTAT	20	7.032835E-4	45.000004	18
GTACGAC	20	7.032835E-4	45.000004	16
TCGCAAT	25	3.8904334E-5	45.0	21
GTCGATG	35	1.2117198E-7	45.0	1
CGAAACG	25	3.8904334E-5	45.0	2
GTTTACG	25	3.8904334E-5	45.0	1
CGCATAC	25	3.8904334E-5	45.0	37
ATTACGC	25	3.8904334E-5	45.0	12
TAACGTG	25	3.8904334E-5	45.0	42
GTCCGTT	25	3.8904334E-5	45.0	41
>>END_MODULE