##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935110.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 997001 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.850580892095394 33.0 31.0 34.0 30.0 34.0 2 32.1776437536171 34.0 31.0 34.0 30.0 34.0 3 32.23764168742058 34.0 31.0 34.0 30.0 34.0 4 35.80671433629455 37.0 35.0 37.0 35.0 37.0 5 30.2959936850615 37.0 35.0 37.0 0.0 37.0 6 32.76160705957165 37.0 35.0 37.0 17.0 37.0 7 34.96136714005302 37.0 35.0 37.0 32.0 37.0 8 35.51154111179427 37.0 35.0 37.0 33.0 37.0 9 37.344678691395494 39.0 37.0 39.0 34.0 39.0 10 37.30588835918921 39.0 37.0 39.0 34.0 39.0 11 37.20530872085384 39.0 37.0 39.0 34.0 39.0 12 37.03916846623022 39.0 37.0 39.0 33.0 39.0 13 36.99681544953315 39.0 37.0 39.0 33.0 39.0 14 38.1014482432816 40.0 37.0 41.0 33.0 41.0 15 38.2422685634217 40.0 37.0 41.0 34.0 41.0 16 38.32284521279317 40.0 37.0 41.0 34.0 41.0 17 38.321995664999335 40.0 37.0 41.0 34.0 41.0 18 38.281341743889925 40.0 37.0 41.0 34.0 41.0 19 38.24260557411677 40.0 37.0 41.0 34.0 41.0 20 38.14500787862801 40.0 37.0 41.0 34.0 41.0 21 38.07563181982766 40.0 36.0 41.0 34.0 41.0 22 38.059865536744695 40.0 36.0 41.0 34.0 41.0 23 37.993039124333876 40.0 36.0 41.0 34.0 41.0 24 37.96641628243101 40.0 36.0 41.0 34.0 41.0 25 37.88513150939668 40.0 36.0 41.0 33.0 41.0 26 37.78359801043329 40.0 36.0 41.0 33.0 41.0 27 37.744873876756394 40.0 36.0 41.0 33.0 41.0 28 37.69478766821698 40.0 36.0 41.0 33.0 41.0 29 37.66797525779813 40.0 36.0 41.0 33.0 41.0 30 37.559005457366645 39.0 36.0 41.0 33.0 41.0 31 32.96409331585425 38.0 33.0 40.0 9.0 41.0 32 33.80437833061351 38.0 33.0 40.0 16.0 41.0 33 35.74180868424405 38.0 34.0 40.0 29.0 41.0 34 36.598517955348086 38.0 35.0 40.0 31.0 41.0 35 36.73105744126636 39.0 35.0 40.0 31.0 41.0 36 36.89541033559645 39.0 35.0 41.0 31.0 41.0 37 37.034871579868025 39.0 35.0 41.0 32.0 41.0 38 36.99629187934616 39.0 35.0 41.0 32.0 41.0 39 36.984975942852614 39.0 35.0 41.0 32.0 41.0 40 36.9502237209391 39.0 35.0 41.0 32.0 41.0 41 36.86528398667604 39.0 35.0 41.0 32.0 41.0 42 36.75300626579111 39.0 35.0 41.0 31.0 41.0 43 36.73048873571842 39.0 35.0 41.0 31.0 41.0 44 36.642156828328154 39.0 35.0 41.0 31.0 41.0 45 36.623286235420025 39.0 35.0 41.0 31.0 41.0 46 36.56693824780517 39.0 35.0 41.0 31.0 41.0 47 36.44817908908818 39.0 35.0 41.0 31.0 41.0 48 36.44603465793916 39.0 35.0 41.0 31.0 41.0 49 36.43109184444148 39.0 35.0 41.0 31.0 41.0 50 36.25554738661245 39.0 35.0 40.0 31.0 41.0 51 35.597091677942146 38.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 7.0 11 6.0 12 7.0 13 16.0 14 21.0 15 24.0 16 60.0 17 122.0 18 293.0 19 487.0 20 885.0 21 1464.0 22 2279.0 23 3614.0 24 5021.0 25 5851.0 26 6861.0 27 8104.0 28 9799.0 29 12067.0 30 15778.0 31 22013.0 32 33616.0 33 39379.0 34 68456.0 35 99751.0 36 116287.0 37 116110.0 38 176330.0 39 252120.0 40 172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.039478395708734 22.060158415086846 25.764668240051915 12.135694949152509 2 31.598162890508636 25.605089663902042 25.687837825639093 17.10890961995023 3 31.1700790671223 24.901078333923437 26.835279001726175 17.093563597228087 4 28.400573319384836 26.54861930930862 26.259953600848945 18.790853770457602 5 21.06056062130329 40.058836450515095 20.992356075871538 17.88824685231008 6 24.748019309910422 32.991040129347915 25.953233748010284 16.307706812731382 7 75.11406708719448 7.6061107260674765 10.726468679570031 6.553353507167997 8 73.65629522939295 6.715138700964192 11.331081914662072 8.297484154980788 9 67.63022303889365 8.786350264443064 14.118641806778529 9.464784889884765 10 37.96555871057301 24.031470379668626 21.446819010211623 16.55615189954674 11 27.28542900157573 25.91913147529441 25.807195780144653 20.988243742985212 12 24.964769343260436 23.544509985446354 30.811904902803505 20.6788157684897 13 23.347719811715333 25.910505606313333 31.4866283985673 19.25514618340403 14 17.74842753417499 30.073189495296393 29.903179635727543 22.27520333480107 15 16.353945482502024 27.605187958688106 34.97569210060973 21.065174458200143 16 18.304093977839543 28.05694277137134 30.34089233611601 23.298070914673104 17 17.902489566209063 27.68191807229882 27.44851810579929 26.967074255692825 18 18.797674225000776 26.485429803982143 31.32504380637532 23.39185216464176 19 19.618134786223884 29.087734114609713 28.647614194970718 22.646516904195682 20 20.981022085233615 29.81992996997997 28.698366400836107 20.500681543950307 21 20.11422255343776 29.791444542182006 30.09575717577013 19.998575728610103 22 18.06668197925579 27.63507759771555 27.50418505096785 26.79405537206081 23 18.125057046081196 28.036581708543924 28.45323124049023 25.385130004884648 24 20.441403769905946 26.30478805939011 28.868677162811274 24.38513100789267 25 17.979119379017675 29.455537155930635 26.205690866909865 26.359652598141825 26 17.14160768143663 30.77238638677394 27.628457744776586 24.45754818701285 27 20.831674190898504 28.499670511865084 27.129160351895333 23.539494945341076 28 18.815828670181876 27.47088518466882 27.888136521427764 25.82514962372154 29 20.07169501334502 27.259551394632503 26.27098668908055 26.39776690294192 30 21.017431276397915 27.044606775720386 25.22545112793267 26.712510819949024 31 25.151529436780905 28.60277973642955 23.034380105937707 23.211310720851834 32 22.070689999307923 29.421033680006335 24.348320613519945 24.15995570716579 33 21.726457646481798 26.508599289268513 24.50950400250351 27.255439061746177 34 20.171594612242114 25.007397184155284 28.987734214910514 25.833273988692085 35 19.64982983968923 26.426352631541995 27.91281051874572 26.011007010023057 36 20.315125060055106 27.917324054840464 26.811106508418746 24.95644437668568 37 22.110910620952236 25.078911656056512 30.22705092572625 22.583126797265 38 22.44691830800571 25.85734618119741 27.138087123282723 24.557648387514156 39 22.974901730289137 24.352733848812587 28.218126160354906 24.45423826054337 40 21.135485320476107 23.00659678375448 31.884220778113566 23.973697117655853 41 17.784535822932977 25.397968507554154 29.750923018131374 27.06657265138149 42 21.23939695145742 25.35764758510774 29.18352138062048 24.21943408281436 43 23.503286355780986 23.696967204646736 28.133371982575746 24.666374456996532 44 21.53347890323079 23.096366001638916 26.92244039875587 28.447714696374426 45 22.114722051432246 21.717631175896514 27.57810674211962 28.589540030551625 46 23.190247552409677 22.18774103536506 29.98131396056774 24.64069745165752 47 18.29045306875319 23.428361656608168 33.01661683388482 25.26456844075382 48 18.647724525853032 22.308001697089573 32.78261506257266 26.261658714484742 49 22.24380918374204 19.996268810161673 32.91200309728877 24.847918908807515 50 20.092356978578756 19.53097338919419 32.97529290341735 27.4013767288097 51 18.548627333372785 19.969588796801606 29.61020099277734 31.871582877048265 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2178.0 1 2335.5 2 2493.0 3 2671.0 4 2849.0 5 2481.0 6 2113.0 7 2139.5 8 2166.0 9 2456.5 10 2747.0 11 2957.5 12 3168.0 13 3470.0 14 3772.0 15 3748.5 16 3725.0 17 3746.0 18 3767.0 19 3790.5 20 3814.0 21 3691.5 22 3569.0 23 3540.0 24 3511.0 25 4627.5 26 6663.0 27 7582.0 28 8921.0 29 10260.0 30 11457.0 31 12654.0 32 13070.0 33 13486.0 34 16492.0 35 19498.0 36 21098.0 37 22698.0 38 24982.5 39 27267.0 40 32674.5 41 38082.0 42 46374.0 43 54666.0 44 66162.5 45 77659.0 46 92186.5 47 106714.0 48 122335.5 49 137957.0 50 132396.0 51 126835.0 52 106087.5 53 85340.0 54 71427.5 55 57515.0 56 49132.5 57 40750.0 58 35247.5 59 29745.0 60 26679.0 61 23613.0 62 20784.5 63 17956.0 64 15597.0 65 13238.0 66 11172.5 67 9107.0 68 8001.0 69 6895.0 70 5432.5 71 3970.0 72 3554.5 73 3139.0 74 2753.0 75 1743.0 76 1119.0 77 921.5 78 724.0 79 484.5 80 245.0 81 232.0 82 219.0 83 150.5 84 82.0 85 42.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 997001.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.31910689555212 #Duplication Level Percentage of deduplicated Percentage of total 1 65.37254498124642 18.512920893582063 2 13.833866027909547 7.835254616462349 3 6.337656711920029 5.384303336765299 4 3.535072440030463 4.0044037725097175 5 2.1665634608761706 3.067757112227482 6 1.4074510315808288 2.3914653728155555 7 0.947494578996941 1.8782540185899366 8 0.7220803272130825 1.6358935978818028 9 0.49448010735122216 1.2602911516023012 >10 4.219320838921046 27.634777530591382 >50 0.8380427053243378 15.57699279048258 >100 0.11224499237266591 5.678747727403331 >500 0.008388415800092443 1.6163746992463504 >1k 0.003994483714329735 2.0725885114137887 >5k 7.98896742865947E-4 1.4499748684260425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT 6923 0.6943824529764765 TruSeq Adapter, Index 22 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC 5895 0.5912732284120076 TruSeq Adapter, Index 20 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC 4926 0.49408175117176417 TruSeq Adapter, Index 20 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC 2182 0.2188563501942325 TruSeq Adapter, Index 20 (95% over 22bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC 1892 0.1897691175836333 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1808 0.1813438502067701 No Hit ACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC 1715 0.17201587561095727 TruSeq Adapter, Index 22 (95% over 21bp) TGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCTT 1312 0.13159465236243495 TruSeq Adapter, Index 20 (95% over 24bp) CGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG 1271 0.12748231947610886 No Hit GCCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG 1118 0.11213629675396514 TruSeq Adapter, Index 20 (95% over 21bp) AAAACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC 1091 0.10942817509711625 No Hit GGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG 1007 0.10100290772025304 TruSeq Adapter, Index 20 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.8216641708483743 0.0 2 0.0 0.0 0.0 2.592775734427548 0.0 3 0.0 0.0 0.0 3.5819422447921316 0.0 4 2.0060160421102888E-4 0.0 0.0 4.760276067927715 0.0 5 2.0060160421102888E-4 0.0 0.0 7.552851000149448 0.0 6 2.0060160421102888E-4 0.0 0.0 9.394173125202482 0.0 7 2.0060160421102888E-4 0.0 0.0 11.004803405412833 0.0 8 2.0060160421102888E-4 0.0 0.0 13.630578103733095 0.0 9 2.0060160421102888E-4 0.0 0.0 14.54100848444485 0.0 10 2.0060160421102888E-4 0.0 0.0 16.418338597453765 0.0 11 2.0060160421102888E-4 0.0 0.0 19.69606850945987 0.0 12 2.0060160421102888E-4 0.0 0.0 22.19686840835666 0.0 13 2.0060160421102888E-4 0.0 0.0 23.142805273013767 0.0 14 2.0060160421102888E-4 0.0 0.0 23.548923220738995 0.0 15 2.0060160421102888E-4 0.0 0.0 24.275000727180814 0.0 16 2.0060160421102888E-4 0.0 0.0 25.756443574279263 0.0 17 2.0060160421102888E-4 0.0 0.0 27.396060786298108 0.0 18 4.0120320842205776E-4 0.0 0.0 29.365467035639885 0.0 19 5.015040105275721E-4 0.0 0.0 30.500571213067992 0.0 20 5.015040105275721E-4 0.0 0.0 31.453027629861957 0.0 21 5.015040105275721E-4 0.0 0.0 32.54309674714469 0.0 22 5.015040105275721E-4 0.0 0.0 33.57569350482096 0.0 23 5.015040105275721E-4 0.0 0.0 34.54760827722339 0.0 24 5.015040105275721E-4 0.0 0.0 35.36114808310122 0.0 25 5.015040105275721E-4 0.0 0.0 36.12042515503997 0.0 26 5.015040105275721E-4 0.0 0.0 36.76044457327525 0.0 27 5.015040105275721E-4 0.0 0.0 37.33135673885984 0.0 28 5.015040105275721E-4 0.0 0.0 37.94138621726558 0.0 29 5.015040105275721E-4 0.0 0.0 38.608988356079884 0.0 30 5.015040105275721E-4 0.0 0.0 39.359639559037554 0.0 31 5.015040105275721E-4 0.0 0.0 40.01129387031708 0.0 32 5.015040105275721E-4 0.0 0.0 40.70497421767882 0.0 33 5.015040105275721E-4 0.0 0.0 41.37668868937945 0.0 34 5.015040105275721E-4 0.0 0.0 42.03957669049479 0.0 35 5.015040105275721E-4 0.0 0.0 42.78300623570087 0.0 36 5.015040105275721E-4 0.0 0.0 43.417609410622454 0.0 37 6.018048126330866E-4 0.0 0.0 44.07488056681989 0.0 38 6.018048126330866E-4 0.0 0.0 44.7225228460152 0.0 39 6.018048126330866E-4 0.0 0.0 45.4081791292085 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACGTA 20 7.032835E-4 45.000004 34 ATATGCG 40 6.8139343E-9 45.000004 1 CATAGGT 20 7.032835E-4 45.000004 19 CTAAACG 20 7.032835E-4 45.000004 1 AACGCAC 75 0.0 45.000004 31 TGCCTCG 20 7.032835E-4 45.000004 29 GTTCACG 20 7.032835E-4 45.000004 1 AACCTCG 20 7.032835E-4 45.000004 17 ACCGTGC 20 7.032835E-4 45.000004 24 GCGCTAC 20 7.032835E-4 45.000004 37 CCGTTAT 20 7.032835E-4 45.000004 18 GTACGAC 20 7.032835E-4 45.000004 16 TCGCAAT 25 3.8904334E-5 45.0 21 GTCGATG 35 1.2117198E-7 45.0 1 CGAAACG 25 3.8904334E-5 45.0 2 GTTTACG 25 3.8904334E-5 45.0 1 CGCATAC 25 3.8904334E-5 45.0 37 ATTACGC 25 3.8904334E-5 45.0 12 TAACGTG 25 3.8904334E-5 45.0 42 GTCCGTT 25 3.8904334E-5 45.0 41 >>END_MODULE