##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935109.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2232268
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0804621129721	33.0	31.0	34.0	30.0	34.0
2	32.41229502909149	34.0	31.0	34.0	30.0	34.0
3	32.46710654813849	34.0	31.0	34.0	30.0	34.0
4	35.96486891358923	37.0	35.0	37.0	35.0	37.0
5	30.41405153861454	37.0	35.0	37.0	0.0	37.0
6	32.89587316576684	37.0	35.0	37.0	17.0	37.0
7	35.10980222804789	37.0	35.0	37.0	32.0	37.0
8	35.718027584501506	37.0	35.0	37.0	35.0	37.0
9	37.6427543646193	39.0	37.0	39.0	35.0	39.0
10	37.50796275357618	39.0	37.0	39.0	35.0	39.0
11	37.338602264602635	39.0	37.0	39.0	35.0	39.0
12	37.01830156594101	39.0	35.0	39.0	34.0	39.0
13	36.86229565625633	39.0	35.0	39.0	33.0	39.0
14	37.62081210679005	40.0	37.0	41.0	32.0	41.0
15	37.851337294625914	40.0	37.0	41.0	33.0	41.0
16	38.10716007217771	40.0	37.0	41.0	33.0	41.0
17	38.193750033598114	40.0	37.0	41.0	34.0	41.0
18	38.177164659440535	40.0	36.0	41.0	34.0	41.0
19	38.17956401292318	40.0	36.0	41.0	34.0	41.0
20	38.07399156373697	40.0	36.0	41.0	33.0	41.0
21	38.039364001096644	40.0	36.0	41.0	34.0	41.0
22	38.094495822186225	40.0	36.0	41.0	34.0	41.0
23	38.06831124219852	40.0	36.0	41.0	34.0	41.0
24	38.040965959284456	40.0	36.0	41.0	34.0	41.0
25	37.97828576138707	40.0	36.0	41.0	34.0	41.0
26	37.924094239580555	40.0	35.0	41.0	34.0	41.0
27	37.88905095624719	40.0	35.0	41.0	34.0	41.0
28	37.8176925888827	40.0	35.0	41.0	34.0	41.0
29	37.79135121768533	40.0	35.0	41.0	34.0	41.0
30	37.68247450574931	40.0	35.0	41.0	33.0	41.0
31	33.03267439214288	38.0	33.0	41.0	9.0	41.0
32	33.92491403361962	38.0	33.0	41.0	16.0	41.0
33	35.86254428231736	38.0	34.0	41.0	29.0	41.0
34	36.66807211320504	38.0	35.0	41.0	31.0	41.0
35	36.82837051823527	39.0	35.0	41.0	31.0	41.0
36	36.92547265830088	39.0	35.0	41.0	31.0	41.0
37	37.023339491494745	39.0	35.0	41.0	32.0	41.0
38	37.020903404071554	39.0	35.0	41.0	32.0	41.0
39	37.01355124026327	39.0	35.0	41.0	32.0	41.0
40	37.03875833905248	39.0	35.0	41.0	32.0	41.0
41	36.936982924989294	39.0	35.0	41.0	32.0	41.0
42	36.86195205951974	39.0	35.0	41.0	32.0	41.0
43	36.86809065936527	39.0	35.0	41.0	32.0	41.0
44	36.8515518745957	39.0	35.0	41.0	32.0	41.0
45	36.86860179870876	39.0	35.0	41.0	32.0	41.0
46	36.87184379294959	39.0	35.0	41.0	32.0	41.0
47	36.75669274477796	39.0	35.0	41.0	32.0	41.0
48	36.71459340903512	38.0	35.0	41.0	32.0	41.0
49	36.68179000012543	38.0	35.0	40.0	32.0	41.0
50	36.50812088871049	38.0	35.0	40.0	32.0	41.0
51	35.86473308760418	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	17.0
11	18.0
12	27.0
13	37.0
14	54.0
15	74.0
16	135.0
17	255.0
18	424.0
19	738.0
20	1245.0
21	1926.0
22	3195.0
23	4870.0
24	7204.0
25	9511.0
26	12185.0
27	15287.0
28	19390.0
29	25700.0
30	36125.0
31	54229.0
32	77880.0
33	96703.0
34	189483.0
35	207175.0
36	249573.0
37	253450.0
38	383029.0
39	581782.0
40	539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10987748782853	20.66750049725212	25.66013579014706	10.562486224772293
2	32.47329621712088	22.813344992626334	25.764513938290563	18.948844851962217
3	32.43279928754074	23.311761849383675	27.202647710758743	17.052791152316836
4	29.56907503937699	24.69963283978447	26.12486493557225	19.606427185266284
5	22.400894516249842	39.00517321396893	22.042962583345727	16.550969686435497
6	27.601300560685367	31.84743050565613	25.08802706485064	15.463241868807867
7	80.71239654020037	5.620740878783372	8.126622789019956	5.540239791996301
8	82.23537675583756	4.113126201692628	8.504086426898562	5.147410615571248
9	76.6244465270299	6.138689440515207	10.606298168499482	6.630565863955404
10	45.346123314942474	23.168006708871875	17.290441828669316	14.195428147516337
11	34.02978495413633	24.606409266270894	23.26472448648639	18.099081293106384
12	30.270648506362143	20.922039826759153	29.00507465949429	19.80223700738442
13	27.997713536188307	22.331010434231015	31.159833855074748	18.51144217450593
14	21.677235887447203	23.931490304927543	34.159966455640635	20.23130735198462
15	20.249674322258798	24.48299218552611	35.50877403609244	19.75855945612265
16	27.402175724420186	24.441196128780234	28.21077039136878	19.945857755430797
17	27.218998794051608	22.964043743851544	26.013811961646184	23.80314550045066
18	27.46148759915924	22.78350986530291	30.070986100235274	19.68401643530257
19	25.834218830355493	25.19101649085146	26.892290710613597	22.082473968179446
20	23.726631390137744	29.79189774704471	27.92299132541433	18.558479537403215
21	23.276595820931895	26.269247240922684	31.553917361177064	18.900239576968357
22	21.912109119514323	22.652118831609826	32.55648515321637	22.879286895659483
23	21.75630345460312	24.703216638862358	31.48730349581681	22.053176410717708
24	22.713356998353245	26.85501920020356	28.354122354484318	22.077501446958877
25	23.694466793413692	26.439164114703072	26.387647002958424	23.478722088924805
26	20.78522829696076	25.59204360766718	27.90529631746726	25.717431777904803
27	22.179594923190226	24.342193679253565	30.278891244241287	23.199320153314925
28	19.73952052352137	25.68894057523559	28.709769615476276	25.861769285766762
29	22.631870366819754	26.674978094028134	28.126282328107553	22.56686921104455
30	26.950482648140817	24.023235561321492	27.732557201913032	21.293724588624663
31	26.954872802011227	25.390141327116638	27.159686919312552	20.49529895155958
32	27.318001243578284	25.37961391732534	26.410045747195227	20.892339091901153
33	28.052545662080004	24.138947474048813	26.452334576314314	21.35617228755687
34	23.33765479772142	23.620371747478348	31.9038305436444	21.13814291115583
35	24.9059700716939	26.62556646424175	27.659089320816317	20.809374143248032
36	27.767723230364812	24.95090195263293	27.530117351500806	19.751257465501453
37	24.269487355460903	27.153056891018462	28.071763784635177	20.505691968885458
38	25.200379165942437	26.019008470309117	27.224240100203023	21.556372263545416
39	24.61707106852761	23.9296984053886	27.681129685145333	23.772100840938453
40	28.12368407377609	21.709490079148203	28.90580342503678	21.26102242203893
41	23.680445179521456	22.597331503206604	27.540689558780578	26.181533758491366
42	23.130152831111676	22.139590765983296	31.901321884289878	22.82893451861515
43	22.989488717304553	22.29853225508765	31.50307221175952	23.208906815848277
44	23.785316100038166	22.49402849478647	29.227046214881007	24.493609190294354
45	23.02272845375197	22.389605549154492	27.321316257725325	27.266349739368213
46	24.43541725276714	22.33419105591264	28.7813560020571	24.44903568926312
47	21.225677203633257	24.007735630309625	31.870456414731564	22.896130751325558
48	22.08386269032213	25.412450476376492	29.657460484135417	22.84622634916596
49	25.151908283414	22.060478401338905	30.48442212135819	22.303191193888907
50	22.778985318967077	23.005436623201156	29.034237824490607	25.18134023334116
51	22.303728763750588	23.104170287796986	27.31159520272655	27.28050574572587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1023.0
1	1693.5
2	2364.0
3	3232.5
4	4101.0
5	3306.5
6	2512.0
7	2526.0
8	2540.0
9	2733.5
10	2927.0
11	2995.0
12	3063.0
13	3239.0
14	3415.0
15	3300.5
16	3186.0
17	3267.0
18	3348.0
19	3714.5
20	4081.0
21	4768.5
22	5456.0
23	6847.0
24	8238.0
25	9326.0
26	12876.0
27	15338.0
28	18442.0
29	21546.0
30	27382.0
31	33218.0
32	36246.5
33	39275.0
34	45033.5
35	50792.0
36	55789.0
37	60786.0
38	66624.0
39	72462.0
40	81858.0
41	91254.0
42	101264.0
43	111274.0
44	126402.5
45	141531.0
46	161532.5
47	181534.0
48	208842.5
49	236151.0
50	238548.5
51	240946.0
52	210831.5
53	180717.0
54	165442.0
55	150167.0
56	140829.5
57	131492.0
58	125047.5
59	118603.0
60	101234.5
61	83866.0
62	74953.5
63	66041.0
64	57343.0
65	48645.0
66	42028.0
67	35411.0
68	30103.5
69	24796.0
70	20489.5
71	16183.0
72	12946.5
73	9710.0
74	7880.5
75	5148.0
76	4245.0
77	3117.5
78	1990.0
79	1529.0
80	1068.0
81	634.5
82	201.0
83	133.0
84	65.0
85	86.5
86	108.0
87	109.5
88	111.0
89	60.5
90	10.0
91	9.0
92	8.0
93	6.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2232268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.937848982954907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.70542807686431	15.96784463647338
2	13.685670387154241	6.552110219163926
3	5.7942293697397815	4.1610416287629866
4	2.976821031890626	2.8503476924272717
5	1.7799679803596793	2.130430235417262
6	1.13640904889302	1.632191291715872
7	0.8533282675811759	1.4298790241571306
8	0.5842845346093046	1.11892119620429
9	0.4646790170612812	1.0011074522364787
>10	4.0412688948803215	21.911897297218594
>50	1.7304354979589562	29.366117088331812
>100	0.2371575618382549	8.012086147645403
>500	0.005265475086280277	0.8476721003560145
>1k	0.004212380069024221	1.6058024383714424
>5k	8.424760138048444E-4	1.4125515515181382
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	8465	0.3792107399290766	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	6848	0.30677320106725536	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	6402	0.286793521208027	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	6302	0.28231377236066635	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	4716	0.21126495564152692	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	2419	0.10836512461765344	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29597700634511626	0.0
2	0.0	0.0	0.0	1.1090066246525956	0.0
3	0.0	0.0	0.0	1.5628947778671736	0.0
4	0.0	0.0	0.0	2.102525324020234	0.0
5	0.0	0.0	0.0	3.517857174855349	0.0
6	0.0	0.0	0.0	4.456812533262135	0.0
7	0.0	0.0	0.0	5.305500952394605	0.0
8	0.0	0.0	0.0	6.674960175032747	0.0
9	0.0	0.0	0.0	7.189011355267378	0.0
10	0.0	0.0	0.0	8.277948705083798	0.0
11	0.0	0.0	0.0	9.972279314132532	0.0
12	0.0	0.0	0.0	11.308588395300205	0.0
13	0.0	0.0	0.0	11.903051067344961	0.0
14	0.0	0.0	0.0	12.151408343442633	0.0
15	0.0	0.0	0.0	12.616719856217982	0.0
16	0.0	0.0	0.0	13.394180268677417	0.0
17	0.0	0.0	0.0	14.31844205086486	0.0
18	0.0	0.0	0.0	15.378485020615804	0.0
19	0.0	0.0	0.0	16.049596195438898	0.0
20	0.0	0.0	0.0	16.704759464365388	0.0
21	0.0	0.0	0.0	17.394551191881977	0.0
22	4.4797488473606214E-5	0.0	0.0	18.138861462870945	0.0
23	4.4797488473606214E-5	0.0	0.0	18.83792627050157	0.0
24	4.4797488473606214E-5	0.0	0.0	19.391892012966185	0.0
25	4.4797488473606214E-5	0.0	0.0	19.943438691053224	0.0
26	4.4797488473606214E-5	0.0	0.0	20.439346888456047	0.0
27	4.4797488473606214E-5	0.0	0.0	21.035019092689588	0.0
28	4.4797488473606214E-5	0.0	0.0	21.551758122232634	0.0
29	4.4797488473606214E-5	0.0	0.0	22.16472215701699	0.0
30	4.4797488473606214E-5	0.0	0.0	22.938464377933116	0.0
31	4.4797488473606214E-5	0.0	0.0	23.46671636201388	0.0
32	8.959497694721243E-5	0.0	0.0	24.072378406177037	0.0
33	8.959497694721243E-5	0.0	0.0	24.662674911793744	0.0
34	2.6878493084163727E-4	0.0	0.0	25.333562099174472	0.0
35	2.6878493084163727E-4	0.0	0.0	26.037689023002613	0.0
36	2.6878493084163727E-4	0.0	0.0	26.638871318318408	0.0
37	2.6878493084163727E-4	0.0	0.0	27.30783221369477	0.0
38	2.6878493084163727E-4	0.0	0.0	28.134928243382962	0.0
39	2.6878493084163727E-4	0.0	0.0	29.6291484714201	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	7.034717E-4	45.0	23
AATTTCG	20	7.034717E-4	45.0	21
CTATCGA	25	3.891992E-5	45.0	28
CGCAATA	35	1.2126111E-7	45.0	14
CCAACGG	105	0.0	42.857143	2
ACGGGTA	275	0.0	40.909092	5
CGTAAGG	365	0.0	40.684933	2
CGTTGTA	95	0.0	40.263153	29
TACCGTA	40	3.4601726E-7	39.375	43
CGTTAGG	255	0.0	38.82353	2
ACGTTAG	35	6.2509353E-6	38.571426	1
CGTTAGT	35	6.2509353E-6	38.571426	30
GCGTTAG	160	0.0	37.96875	1
TAGCACG	95	0.0	37.894733	1
ACTTGAC	1060	0.0	37.78302	6
CCGTAAA	30	1.1402663E-4	37.500004	13
TGCATTA	865	0.0	37.456646	15
CACGGGA	705	0.0	37.340424	4
ATAACGG	255	0.0	37.058823	2
CTACGGG	675	0.0	37.000004	3
>>END_MODULE