##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935108.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1935765
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.063725710507214	33.0	31.0	34.0	30.0	34.0
2	32.39092090207231	34.0	31.0	34.0	30.0	34.0
3	32.44374549596671	34.0	31.0	34.0	30.0	34.0
4	35.94253434688611	37.0	35.0	37.0	35.0	37.0
5	30.41775732074916	37.0	35.0	37.0	0.0	37.0
6	32.88953462842856	37.0	35.0	37.0	17.0	37.0
7	35.07082781226027	37.0	35.0	37.0	32.0	37.0
8	35.59507791493286	37.0	35.0	37.0	35.0	37.0
9	37.472270136096064	39.0	37.0	39.0	35.0	39.0
10	37.416072715438084	39.0	37.0	39.0	35.0	39.0
11	37.31925982751005	39.0	37.0	39.0	35.0	39.0
12	37.182466880019014	39.0	37.0	39.0	34.0	39.0
13	37.12533959442391	39.0	37.0	39.0	33.0	39.0
14	38.33056440218725	40.0	38.0	41.0	34.0	41.0
15	38.4280679731269	40.0	38.0	41.0	34.0	41.0
16	38.52090517185712	40.0	38.0	41.0	34.0	41.0
17	38.50655838906066	40.0	38.0	41.0	34.0	41.0
18	38.4736788814758	40.0	38.0	41.0	34.0	41.0
19	38.45672951003867	40.0	37.0	41.0	34.0	41.0
20	38.3912773502982	40.0	37.0	41.0	34.0	41.0
21	38.349124506332124	40.0	37.0	41.0	34.0	41.0
22	38.32923882806023	40.0	37.0	41.0	34.0	41.0
23	38.25614989422786	40.0	37.0	41.0	34.0	41.0
24	38.23279013723257	40.0	37.0	41.0	34.0	41.0
25	38.15944859009229	40.0	37.0	41.0	34.0	41.0
26	38.060684535571205	40.0	37.0	41.0	34.0	41.0
27	38.009488238500026	40.0	36.0	41.0	34.0	41.0
28	37.94153732503687	40.0	36.0	41.0	34.0	41.0
29	37.91966018602464	40.0	36.0	41.0	34.0	41.0
30	37.81972191872464	40.0	36.0	41.0	33.0	41.0
31	33.21969402277652	39.0	33.0	41.0	9.0	41.0
32	34.121880496857834	39.0	33.0	41.0	16.0	41.0
33	36.08410060105437	39.0	34.0	41.0	30.0	41.0
34	36.928887029159014	39.0	35.0	41.0	31.0	41.0
35	37.09891541586918	39.0	35.0	41.0	32.0	41.0
36	37.25255028373795	39.0	35.0	41.0	33.0	41.0
37	37.368933729042524	39.0	35.0	41.0	33.0	41.0
38	37.333548235452135	39.0	35.0	41.0	33.0	41.0
39	37.35307384935671	39.0	35.0	41.0	33.0	41.0
40	37.32059366710319	39.0	35.0	41.0	33.0	41.0
41	37.25807368146443	39.0	35.0	41.0	33.0	41.0
42	37.15386010182021	39.0	35.0	41.0	33.0	41.0
43	37.1443770292365	39.0	35.0	41.0	33.0	41.0
44	37.08713712666569	39.0	35.0	41.0	33.0	41.0
45	37.05954390124835	39.0	35.0	41.0	33.0	41.0
46	37.007463199303636	39.0	35.0	41.0	32.0	41.0
47	36.920688203371796	39.0	35.0	41.0	32.0	41.0
48	36.90748257148982	39.0	35.0	41.0	32.0	41.0
49	36.89528945920605	39.0	35.0	41.0	32.0	41.0
50	36.7175023827789	39.0	35.0	41.0	32.0	41.0
51	36.121952303094645	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	12.0
10	6.0
11	15.0
12	21.0
13	19.0
14	35.0
15	58.0
16	91.0
17	197.0
18	338.0
19	671.0
20	1070.0
21	1792.0
22	3088.0
23	4787.0
24	6821.0
25	8725.0
26	10594.0
27	12960.0
28	15879.0
29	20376.0
30	26931.0
31	38613.0
32	59411.0
33	69026.0
34	127142.0
35	184251.0
36	227003.0
37	227891.0
38	336429.0
39	550999.0
40	514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30952620798495	21.334356184764165	25.563123622960433	10.79299398429045
2	31.488842912233668	25.541271796938158	24.60753242258229	18.36235286824589
3	32.40176364383073	25.00535963817922	24.793040477537303	17.79983624045274
4	28.69341061544144	25.214992522336132	26.451299615397527	19.640297246824897
5	23.520675288581	38.84748406960556	19.978199833140902	17.65364080867254
6	26.62125826223741	34.840592737238254	22.34858053534391	16.189568465180432
7	78.84087169671939	6.458635216568127	9.065924841083499	5.634568245628989
8	79.16637608387381	6.704377855783114	8.180125170152369	5.9491208901907
9	73.21839169527293	7.8314258187331625	10.760035438185938	8.190147047807972
10	40.84865673260959	26.125588591590404	17.18628035944446	15.83947431635555
11	31.40747973023585	25.755399028291144	23.135091294656117	19.70202994681689
12	27.873837991698373	22.073960423915093	28.314438994402728	21.737762589983806
13	25.25451178216364	24.06593775587429	30.148804219520446	20.53074624244162
14	20.273845223981215	28.43650959698104	28.396732041337664	22.892913137700084
15	18.862155271946747	25.97505379010365	33.73141884474614	21.431372093203464
16	21.66781608304727	25.871012235472797	29.74513951848494	22.716032162994992
17	21.210942443943352	25.861042016980367	27.43199716907786	25.496018369998424
18	22.300537513592815	25.016776313240502	29.27302642624492	23.40965974692176
19	22.601400479913625	26.8936053704866	27.663275242604346	22.841718906995425
20	25.61917381500337	26.513290611205388	26.95538972964177	20.91214584414947
21	23.61526321635116	27.589144343450776	27.346811209005224	21.448781231192836
22	22.289430793510576	25.049734859345012	27.19214367446462	25.468690672679795
23	21.81173851164785	26.756811906403925	27.83137415956999	23.600075422378232
24	22.70781835605045	24.73983153946889	28.08620881150346	24.466141292977195
25	21.6727753627119	28.053146947072605	25.867447753213845	24.40662993700165
26	21.67902612145586	28.093027821042327	26.68361087218748	23.54433518531433
27	23.00547845425452	26.97207563934672	27.26772103018703	22.75472487621173
28	20.150483142323576	27.271492149098677	28.710613116778124	23.867411591799627
29	21.509687384574057	26.198996262459545	27.481073374092414	24.810242978873987
30	23.421283058635733	25.923859559399	27.230319796049624	23.424537585915644
31	24.261984280116646	27.420477175690234	24.279755032248232	24.03778351194489
32	22.51616286067782	27.151591231373644	25.687363910392015	24.644881997556524
33	22.8951861408797	24.711057385581412	27.084279341758943	25.309477131779946
34	20.664336838407554	25.340369311357524	27.801670140745387	26.19362370948953
35	21.122243660774938	25.13047813138475	26.40155184126172	27.34572636657859
36	21.74442662203315	26.621516558053276	28.34269655665848	23.291360263255097
37	21.965837795393554	25.699245517921852	28.282668609051203	24.05224807763339
38	20.72142021371396	25.243611698734092	27.446823348908573	26.588144738643376
39	21.753208679772595	22.94343579928349	29.303298695864427	26.000056825079493
40	21.808742280183804	23.346893863666303	29.475013754252195	25.369350101897698
41	19.830196330649642	24.764834574444727	27.35388851435996	28.051080580545673
42	21.06924135935922	24.571061053381996	28.829687487892386	25.5300100993664
43	22.483204314573307	23.543456979540387	29.354854540711294	24.61848416517501
44	21.141719165291242	23.708559665041985	28.276056236165033	26.87366493350174
45	21.19038209700041	22.46842979390577	27.327025749509883	29.01416235958394
46	22.358964027141724	24.012625499479533	27.900080846590363	25.728329626788376
47	19.625264430341495	23.883632569036013	31.031246044845318	25.45985695577717
48	19.725069933592145	22.82090026423662	30.21596113164563	27.238068670525607
49	21.319788300749316	21.68274558120433	31.55853112335433	25.43893499469202
50	19.840166549142072	21.95307798208977	30.867848111728435	27.338907357039723
51	18.911076499471786	22.067606346844787	28.14845810312719	30.87285905055624
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1098.0
1	1774.5
2	2451.0
3	3128.5
4	3806.0
5	3290.5
6	2775.0
7	2679.0
8	2583.0
9	2761.0
10	2939.0
11	3295.5
12	3652.0
13	3749.5
14	3847.0
15	4089.0
16	4331.0
17	4360.0
18	4389.0
19	4466.0
20	4543.0
21	5452.5
22	6362.0
23	6878.5
24	7395.0
25	9178.5
26	12809.0
27	14656.0
28	18457.0
29	22258.0
30	24878.5
31	27499.0
32	31097.0
33	34695.0
34	39624.0
35	44553.0
36	47532.5
37	50512.0
38	54285.5
39	58059.0
40	67354.0
41	76649.0
42	86937.5
43	97226.0
44	112028.0
45	126830.0
46	154186.0
47	181542.0
48	194189.5
49	206837.0
50	201689.0
51	196541.0
52	173558.5
53	150576.0
54	132734.0
55	114892.0
56	105301.0
57	95710.0
58	90877.0
59	86044.0
60	83219.5
61	80395.0
62	72654.0
63	64913.0
64	57152.0
65	49391.0
66	40173.5
67	30956.0
68	26649.0
69	22342.0
70	18417.0
71	14492.0
72	12255.5
73	10019.0
74	8679.5
75	6338.0
76	5336.0
77	3808.0
78	2280.0
79	1729.5
80	1179.0
81	876.0
82	573.0
83	407.0
84	241.0
85	164.5
86	88.0
87	47.0
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1935765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.663400375909696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.66779131146775	15.775866379808551
2	13.489450037222689	6.384125141632609
3	6.013200433043537	4.268783081631083
4	3.194186860309827	3.023412902039255
5	1.939437445314553	2.2946842386254866
6	1.2565028505538685	1.783987801567681
7	0.8572169684654065	1.4199267833674332
8	0.6007082018575332	1.137183895171807
9	0.44553054511276446	0.9488490901830577
>10	3.4453305623445862	18.55796761695399
>50	1.7384390352298857	29.700725260779244
>100	0.3410834630157528	10.895410629955704
>500	0.005931885899666314	0.9728128227370881
>1k	0.004448914424749735	1.8252595786676515
>5k	7.414857374582893E-4	1.0110047768792945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	6173	0.31889201426826086	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	5736	0.29631695996156554	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	5377	0.2777713203823811	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC	4146	0.21417889051615252	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG	3703	0.19129388123041796	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	2760	0.14257929035807548	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	2238	0.11561320718165685	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5497748951964727E-4	0.0	0.0	0.3370760397052328	0.0
2	1.5497748951964727E-4	0.0	0.0	1.1581984383434973	0.0
3	1.5497748951964727E-4	0.0	0.0	1.6406950223813324	0.0
4	1.5497748951964727E-4	0.0	0.0	2.266700761714361	0.0
5	1.5497748951964727E-4	0.0	0.0	3.8669466593310653	0.0
6	1.5497748951964727E-4	0.0	0.0	4.905140861623183	0.0
7	1.5497748951964727E-4	0.0	0.0	5.790682236738447	0.0
8	1.5497748951964727E-4	0.0	0.0	7.291070971941325	0.0
9	1.5497748951964727E-4	0.0	0.0	7.809832288526758	0.0
10	1.5497748951964727E-4	0.0	0.0	9.151524074461518	0.0
11	1.5497748951964727E-4	0.0	0.0	10.966930386694665	0.0
12	1.5497748951964727E-4	0.0	0.0	12.666258559277598	0.0
13	1.5497748951964727E-4	0.0	0.0	13.277179822964047	0.0
14	2.0663665269286303E-4	0.0	0.0	13.530774654981364	0.0
15	2.0663665269286303E-4	0.0	0.0	14.039565753074365	0.0
16	2.0663665269286303E-4	0.0	0.0	14.953158053792686	0.0
17	2.0663665269286303E-4	0.0	0.0	16.01713017850824	0.0
18	2.5829581586607876E-4	0.0	0.0	17.231327149731502	0.0
19	2.5829581586607876E-4	0.0	0.0	18.036745162765108	0.0
20	2.5829581586607876E-4	0.0	0.0	18.70196020694661	0.0
21	2.5829581586607876E-4	0.0	0.0	19.430974317647028	0.0
22	2.5829581586607876E-4	0.0	0.0	20.19258536030975	0.0
23	2.5829581586607876E-4	0.0	0.0	20.920411310257187	0.0
24	2.5829581586607876E-4	0.0	0.0	21.530506027332862	0.0
25	2.5829581586607876E-4	0.0	0.0	22.09204113102572	0.0
26	2.5829581586607876E-4	0.0	0.0	22.57996192719674	0.0
27	2.5829581586607876E-4	0.0	0.0	23.06235519290823	0.0
28	2.5829581586607876E-4	0.0	0.0	23.55084423987416	0.0
29	2.5829581586607876E-4	0.0	0.0	24.08649810281723	0.0
30	2.5829581586607876E-4	0.0	0.0	24.643125586008633	0.0
31	3.0995497903929455E-4	0.0	0.0	25.17836617564632	0.0
32	3.0995497903929455E-4	0.0	0.0	25.769398661511083	0.0
33	3.0995497903929455E-4	0.0	0.0	26.340335732901462	0.0
34	3.0995497903929455E-4	0.0	0.0	26.895516759524014	0.0
35	3.0995497903929455E-4	0.0	0.0	27.495227984801875	0.0
36	3.616141422125103E-4	0.0	0.0	28.088430155519912	0.0
37	3.616141422125103E-4	0.0	0.0	28.682665509501412	0.0
38	4.649324685589418E-4	0.0	0.0	29.260266612941138	0.0
39	4.649324685589418E-4	0.0	0.0	29.842052108597894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAT	20	7.0344826E-4	45.000004	18
AACGATC	20	7.0344826E-4	45.000004	26
TCGTTAC	35	1.212502E-7	45.0	20
ATATGCG	35	1.212502E-7	45.0	1
CAACACG	25	3.891799E-5	45.0	40
TCCCGTA	25	3.891799E-5	45.0	18
CTGTCGT	55	6.184564E-11	40.90909	17
ACGCTTA	50	1.0822987E-9	40.5	17
GTCGTGA	45	1.929402E-8	40.0	25
TACGACG	80	0.0	39.375004	1
TCGACGT	40	3.4598634E-7	39.375004	26
TATACGG	200	0.0	39.375	2
TACGGGA	755	0.0	39.33775	4
TCACGAG	35	6.250506E-6	38.571426	23
GCGATAT	70	0.0	38.571426	9
CATACGA	305	0.0	37.622948	18
GCGCGAT	30	1.14021044E-4	37.500004	22
CCTACGG	30	1.14021044E-4	37.500004	2
TGCGCAA	30	1.14021044E-4	37.500004	1
GATTCGT	30	1.14021044E-4	37.500004	26
>>END_MODULE