##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935107.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2105275
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95060217786275	33.0	31.0	34.0	30.0	34.0
2	32.27871798221135	34.0	31.0	34.0	30.0	34.0
3	32.35507047772856	34.0	31.0	34.0	30.0	34.0
4	35.898689244873	37.0	35.0	37.0	35.0	37.0
5	30.441487216634407	37.0	35.0	37.0	0.0	37.0
6	32.856712780990605	37.0	35.0	37.0	17.0	37.0
7	35.00892804977972	37.0	35.0	37.0	32.0	37.0
8	35.43629454584318	37.0	35.0	37.0	33.0	37.0
9	37.42283407155835	39.0	37.0	39.0	35.0	39.0
10	37.35388820937882	39.0	37.0	39.0	34.0	39.0
11	37.212032157319115	39.0	37.0	39.0	34.0	39.0
12	36.94545320682571	39.0	35.0	39.0	33.0	39.0
13	36.757145266057876	39.0	35.0	39.0	33.0	39.0
14	37.59188134566743	40.0	36.0	41.0	32.0	41.0
15	37.718334184370214	40.0	36.0	41.0	32.0	41.0
16	38.02329481896664	40.0	37.0	41.0	33.0	41.0
17	37.99016043034758	40.0	36.0	41.0	33.0	41.0
18	38.01815724786548	40.0	36.0	41.0	33.0	41.0
19	38.005602118488085	40.0	36.0	41.0	33.0	41.0
20	37.7743933690373	40.0	36.0	41.0	33.0	41.0
21	37.84828110341879	40.0	36.0	41.0	33.0	41.0
22	37.90688627376471	40.0	36.0	41.0	33.0	41.0
23	37.765366994810655	40.0	36.0	41.0	33.0	41.0
24	37.75529135148615	40.0	35.0	41.0	33.0	41.0
25	37.74689007374334	40.0	35.0	41.0	33.0	41.0
26	37.692144731685886	40.0	35.0	41.0	33.0	41.0
27	37.571996532519506	39.0	35.0	41.0	33.0	41.0
28	37.50735984610087	39.0	35.0	41.0	33.0	41.0
29	37.47353148638539	39.0	35.0	41.0	33.0	41.0
30	37.403002458111175	39.0	35.0	41.0	33.0	41.0
31	32.900204011352436	38.0	33.0	40.0	9.0	41.0
32	33.693058151547895	38.0	33.0	40.0	16.0	41.0
33	35.58450748714539	38.0	34.0	40.0	27.0	41.0
34	36.32648703851041	38.0	35.0	40.0	30.0	41.0
35	36.59305458906794	39.0	35.0	40.0	31.0	41.0
36	36.68880027549845	39.0	35.0	40.0	31.0	41.0
37	36.76183776466257	39.0	35.0	41.0	31.0	41.0
38	36.768268278490936	39.0	35.0	41.0	31.0	41.0
39	36.80168529052024	39.0	35.0	41.0	31.0	41.0
40	36.843641329517524	39.0	35.0	41.0	31.0	41.0
41	36.65093538848844	39.0	35.0	41.0	31.0	41.0
42	36.64562871833846	39.0	35.0	41.0	31.0	41.0
43	36.667199297003954	39.0	35.0	41.0	31.0	41.0
44	36.688890999988125	39.0	35.0	41.0	31.0	41.0
45	36.63275961572716	39.0	35.0	41.0	31.0	41.0
46	36.60971037037917	39.0	35.0	41.0	31.0	41.0
47	36.448398902756175	38.0	35.0	40.0	31.0	41.0
48	36.49215660661909	38.0	35.0	40.0	31.0	41.0
49	36.53260215411288	39.0	35.0	40.0	31.0	41.0
50	36.326806711712244	38.0	35.0	40.0	31.0	41.0
51	35.72904347413046	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	7.0
11	12.0
12	19.0
13	29.0
14	30.0
15	64.0
16	107.0
17	193.0
18	421.0
19	750.0
20	1602.0
21	3199.0
22	5435.0
23	8472.0
24	11086.0
25	13322.0
26	15167.0
27	17406.0
28	20319.0
29	26440.0
30	36450.0
31	53691.0
32	77258.0
33	92904.0
34	170404.0
35	195234.0
36	234879.0
37	237778.0
38	351571.0
39	530476.0
40	544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.957392739665835	19.226229352459892	22.412131431760695	10.404246476113572
2	29.557041241643017	30.637232665566255	23.082210162567833	16.72351593022289
3	29.591431048200356	30.664972509529637	23.81836102171925	15.92523542055076
4	27.047772856277685	23.32588379190367	31.68982674472456	17.936516607094084
5	26.9270760351973	36.61170155917873	20.034959803351107	16.42626260227286
6	24.99640189523934	36.86416263908516	23.742408948949663	14.397026516725845
7	74.19292016482407	5.430739452090582	15.259146667299998	5.117193715785349
8	73.7801949864032	10.970300792058046	9.116576219258766	6.1329280022799875
9	68.7337283727779	6.034033558561233	10.644405125221171	14.587832943439693
10	45.57827362221087	20.826637850162093	17.86621700252936	15.72887152509767
11	35.11631497072829	21.831019700514183	23.936920354823005	19.11574497393452
12	28.646091365736066	19.903053045326622	28.138651720084074	23.31220386885324
13	28.111909370509792	20.90515490850364	33.465651755708876	17.517283965277695
14	21.24169051549085	26.771466910498624	30.950588402940234	21.036254171070286
15	18.40529147023548	22.62497773450024	37.36504732160882	21.604683473655463
16	23.21967438933156	24.455237439289405	26.53928821650378	25.785799954875255
17	23.800928619776514	25.17761337592476	28.489912244243627	22.5315457600551
18	24.452529954519004	22.88157129116149	29.53775635012053	23.128142404198975
19	23.036420420135137	26.16855280189049	26.629917706712902	24.165109071261472
20	23.18134210495066	28.30485328520027	30.353849259597915	18.159955350251156
21	22.02505610905939	29.60477847312109	29.963686454263694	18.40647896355583
22	19.873318212585055	22.01740865207633	33.371222286874634	24.738050848463978
23	20.686846136490484	26.839344028689837	30.64996259396041	21.82384724085927
24	21.939034092933227	26.192587666694372	27.353101138806096	24.515277101566305
25	21.022526748287042	30.785526831411573	24.66984123214307	23.522105188158314
26	19.759651351961143	24.851812708553513	28.831292823977865	26.55724311550747
27	23.265511631497073	25.16683093657598	28.02132738003349	23.546330051893456
28	17.86844948997162	26.716937217228153	29.446794361781713	25.96781893101851
29	22.790514303357043	26.29480709171011	26.779731864008266	24.134946740924583
30	25.180130861763907	26.669342484948523	27.06924273550961	21.081283917777963
31	26.241844889622495	24.167341558703733	26.128510527128284	23.462303024545488
32	29.522176437757537	26.05251095462588	24.0098324446925	20.415480162924084
33	25.70704539786964	22.811034187932695	24.27663844390875	27.20528197028892
34	22.32705941029082	25.084418900143685	30.404578974243268	22.183942715322228
35	26.59918537958224	25.51771146287302	25.421192005794968	22.46191115174977
36	23.35694861716403	29.963733953996506	25.428506964648324	21.25081046419114
37	25.378537245727994	25.606298464571136	28.45409744570187	20.561066843999
38	24.03467480495422	24.387122822434122	26.167412808302952	25.4107895643087
39	24.436665043759128	24.518222085000772	27.026207977580125	24.018904893659972
40	25.20497322202563	21.442234387431572	27.926422913871107	25.426369476671695
41	21.42290199617627	25.79572739903338	26.662027526095166	26.119343078695184
42	23.958485233520562	22.882853783947464	30.369571671159353	22.789089311372624
43	22.364916697343578	25.68391302798922	27.99757751362649	23.953592761040717
44	24.09932194131408	21.84118464333638	28.970087043260385	25.089406372089158
45	21.86384201588866	20.39595777273753	27.867238246784865	29.872961964588953
46	25.97470639227654	23.831660946907174	26.69456484307276	23.499067817743523
47	18.74372706653525	23.64802697984824	34.48513852109582	23.123107432520694
48	20.96353208013205	25.708327890655614	28.859032667941243	24.469107361271092
49	24.347033047939107	20.76146821674128	31.691964232701192	23.199534502618423
50	22.03294106470651	22.466328626901475	28.75130327391908	26.749427034472934
51	20.967997055016564	22.038213535048865	26.74291957107741	30.25086983885716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	949.0
1	1520.5
2	2092.0
3	3089.5
4	4087.0
5	3253.0
6	2419.0
7	2381.0
8	2343.0
9	2471.0
10	2599.0
11	2748.5
12	2898.0
13	3009.5
14	3121.0
15	3279.5
16	3438.0
17	3228.5
18	3019.0
19	3418.5
20	3818.0
21	4679.0
22	5540.0
23	6081.0
24	6622.0
25	8165.0
26	11669.5
27	13631.0
28	16644.0
29	19657.0
30	22819.0
31	25981.0
32	30052.5
33	34124.0
34	39100.0
35	44076.0
36	48531.5
37	52987.0
38	57535.5
39	62084.0
40	67110.5
41	72137.0
42	84925.5
43	97714.0
44	117109.0
45	136504.0
46	204607.5
47	272711.0
48	252659.5
49	232608.0
50	226501.5
51	220395.0
52	193698.5
53	167002.0
54	150619.0
55	134236.0
56	126634.0
57	119032.0
58	108361.5
59	97691.0
60	84881.0
61	72071.0
62	63709.5
63	55348.0
64	47768.0
65	40188.0
66	34011.0
67	27834.0
68	23697.0
69	19560.0
70	16087.0
71	12614.0
72	10955.5
73	9297.0
74	7490.0
75	4738.5
76	3794.0
77	2769.5
78	1745.0
79	1444.5
80	1144.0
81	891.5
82	639.0
83	364.0
84	89.0
85	53.0
86	17.0
87	22.5
88	28.0
89	14.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2105275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.315496223290054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.57001022577313	16.42998328452457
2	13.318380354865262	6.476860544381335
3	5.702034751520526	4.159434133969979
4	2.958453158465452	2.877450264057889
5	1.7328269006347838	2.106727297900025
6	1.1082846828891812	1.616909521267326
7	0.7875433008871686	1.3404654310879531
8	0.5990103557388688	1.1652187234144078
9	0.45783429204109377	1.0019221199116102
>10	4.153276145841201	23.48878666456369
>50	1.4608726653797255	24.24981904176424
>100	0.1380820679368477	5.235403276688013
>500	0.006805312111969058	1.120903188888326
>1k	0.0043905239432058445	1.9033308731935352
>5k	0.0013171571829617532	1.9115512213572001
>10k+	8.781047886411689E-4	4.915234413029899
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	31789	1.509969006424339	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	29323	1.3928346653050077	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	20639	0.9803469855482062	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT	10331	0.4907197397014642	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	8344	0.3963377706000404	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	6065	0.2880858795169277	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT	5718	0.2716034722304687	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	5344	0.25383857215803163	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	5249	0.2493260975407013	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTA	5091	0.24182113975608888	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	4669	0.22177625250857963	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	3900	0.18524895797461138	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2812	0.1335692486729762	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC	2178	0.10345441806889837	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4249919844200876E-4	0.0	0.0	0.2845708992886915	0.0
2	1.4249919844200876E-4	0.0	0.0	1.1742883946277802	0.0
3	1.4249919844200876E-4	0.0	0.0	1.6229708707888517	0.0
4	1.4249919844200876E-4	0.0	0.0	2.3350393654035697	0.0
5	1.4249919844200876E-4	0.0	0.0	4.723658429421334	0.0
6	1.4249919844200876E-4	0.0	0.0	5.702912921114819	0.0
7	1.4249919844200876E-4	0.0	0.0	6.868128866775124	0.0
8	1.4249919844200876E-4	0.0	0.0	8.506252152331642	0.0
9	1.4249919844200876E-4	0.0	0.0	8.9935519112705	0.0
10	1.4249919844200876E-4	0.0	0.0	11.733763997577514	0.0
11	1.4249919844200876E-4	0.0	0.0	13.38870218855019	0.0
12	1.4249919844200876E-4	0.0	0.0	16.571184287088386	0.0
13	1.4249919844200876E-4	0.0	0.0	17.205210720689696	0.0
14	1.8999893125601168E-4	0.0	0.0	17.54507130897389	0.0
15	1.8999893125601168E-4	0.0	0.0	18.274477206065715	0.0
16	1.8999893125601168E-4	0.0	0.0	19.209081948914037	0.0
17	1.8999893125601168E-4	0.0	0.0	20.143639192029546	0.0
18	1.8999893125601168E-4	0.0	0.0	21.09382384724086	0.0
19	1.8999893125601168E-4	0.0	0.0	22.43887378133498	0.0
20	1.8999893125601168E-4	0.0	0.0	23.107907518020212	0.0
21	1.8999893125601168E-4	0.0	0.0	23.80097611950933	0.0
22	2.374986640700146E-4	0.0	0.0	24.568381802852358	0.0
23	2.374986640700146E-4	0.0	0.0	25.239030530453267	0.0
24	2.374986640700146E-4	0.0	0.0	25.830877201315744	0.0
25	2.374986640700146E-4	0.0	0.0	26.368906674899954	0.0
26	2.374986640700146E-4	0.0	0.0	26.85976891379986	0.0
27	2.374986640700146E-4	0.0	0.0	27.472942964695825	0.0
28	2.374986640700146E-4	0.0	0.0	27.98028761088219	0.0
29	2.374986640700146E-4	0.0	0.0	28.568904299913314	0.0
30	2.374986640700146E-4	0.0	0.0	29.24145301682678	0.0
31	2.374986640700146E-4	0.0	0.0	29.725142796071772	0.0
32	2.374986640700146E-4	0.0	0.0	30.282742159575353	0.0
33	2.374986640700146E-4	0.0	0.0	30.849746470176104	0.0
34	2.374986640700146E-4	0.0	0.0	31.486480388547815	0.0
35	2.374986640700146E-4	0.0	0.0	32.126444288750875	0.0
36	2.374986640700146E-4	0.0	0.0	32.68252366080441	0.0
37	2.374986640700146E-4	0.0	0.0	33.295745211433186	0.0
38	2.374986640700146E-4	0.0	0.0	34.05479094180095	0.0
39	2.374986640700146E-4	0.0	0.0	35.29814394794029	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	20	7.034626E-4	45.0	21
ACACGAA	25	3.8919163E-5	45.0	31
CTACCGT	25	3.8919163E-5	45.0	36
CGTACAA	20	7.034626E-4	45.0	30
CGGTCGA	20	7.034626E-4	45.0	13
TCGAATA	40	6.8193913E-9	45.0	44
CGATCGA	290	0.0	42.672413	41
GCTAACG	90	0.0	42.5	1
TAATACG	1150	0.0	42.45652	4
TACGGCT	4385	0.0	41.92132	7
TTGCACG	180	0.0	41.25	1
AATACGG	1190	0.0	41.02941	5
CTAACGC	315	0.0	40.714283	29
ACGGGTA	255	0.0	40.588234	5
ATAATAC	1820	0.0	40.54945	3
TCTAACG	315	0.0	39.999996	28
CGTAAGG	220	0.0	39.886368	2
ACGGCTG	4645	0.0	39.865444	8
TAGGTCG	85	0.0	39.705883	1
CGGTCTA	85	0.0	39.705883	31
>>END_MODULE