SRR2935106.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935106.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1731907
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	11809	0.6818495450390812	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	10785	0.6227239684347947	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	9935	0.5736451206675647	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	9564	0.5522236471126915	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	8860	0.5115748131972444	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	7240	0.4180363033349943	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3405	0.19660409017343308	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2986	0.17241110521523384	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	2735	0.15791841016867533	No Hit
TGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCTT	2677	0.15456950055632315	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2671	0.15422306163090743	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	2189	0.12639246795584289	No Hit
GGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1908	0.11016757828220568	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	1895	0.10941696061047157	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	1874	0.10820442437151649	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1873	0.10814668455061385	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1866	0.1077425058042955	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1836	0.10601031117721679	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	1741	0.10052502819146755	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	35	1.212411E-7	45.000004	1
ACCGCGT	20	7.034277E-4	45.000004	22
TAACGCG	20	7.034277E-4	45.000004	1
CCGTCGC	20	7.034277E-4	45.000004	19
CGTAGCG	20	7.034277E-4	45.000004	2
CGTCGTC	20	7.034277E-4	45.000004	16
CGGTCAT	20	7.034277E-4	45.000004	17
GCCGTAG	20	7.034277E-4	45.000004	1
GCGTACC	20	7.034277E-4	45.000004	13
TACGTCG	20	7.034277E-4	45.000004	14
CCCGTTT	25	3.8916274E-5	45.0	15
CCCGTAG	25	3.8916274E-5	45.0	44
TCCCGTA	25	3.8916274E-5	45.0	43
ATAATCG	115	0.0	43.04348	30
AACCGTA	55	6.184564E-11	40.909092	3
GATTACG	110	0.0	40.909092	1
CGTTTTT	1400	0.0	40.17857	1
TGCCGCG	130	0.0	39.80769	1
CGCTCGT	85	0.0	39.705883	11
ACGGTCC	245	0.0	39.489796	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content