##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935106.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1731907
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848700305501392	33.0	31.0	34.0	30.0	34.0
2	32.16767643990122	33.0	31.0	34.0	30.0	34.0
3	32.22722986857839	34.0	31.0	34.0	30.0	34.0
4	35.806032310048984	37.0	35.0	37.0	35.0	37.0
5	30.293832174591362	37.0	35.0	37.0	0.0	37.0
6	32.75986297185703	37.0	35.0	37.0	17.0	37.0
7	34.975964644752864	37.0	35.0	37.0	32.0	37.0
8	35.503781092171806	37.0	35.0	37.0	33.0	37.0
9	37.421184855768814	39.0	37.0	39.0	35.0	39.0
10	37.24434395149393	39.0	37.0	39.0	34.0	39.0
11	37.189790213908715	39.0	37.0	39.0	34.0	39.0
12	37.12380456918299	39.0	37.0	39.0	34.0	39.0
13	37.10185708585969	39.0	37.0	39.0	33.0	39.0
14	38.2356229289448	40.0	38.0	41.0	33.0	41.0
15	38.28907210375615	40.0	38.0	41.0	34.0	41.0
16	38.39513842255964	40.0	38.0	41.0	34.0	41.0
17	38.35930566710568	40.0	38.0	41.0	34.0	41.0
18	38.30989250577542	40.0	37.0	41.0	34.0	41.0
19	38.276560461964756	40.0	37.0	41.0	34.0	41.0
20	38.191272972509495	40.0	37.0	41.0	34.0	41.0
21	38.167736489314954	40.0	37.0	41.0	34.0	41.0
22	38.18729643104393	40.0	37.0	41.0	34.0	41.0
23	38.09584406091089	40.0	37.0	41.0	34.0	41.0
24	38.07652951342075	40.0	37.0	41.0	34.0	41.0
25	38.04049178160259	40.0	37.0	41.0	34.0	41.0
26	38.00420230416529	40.0	37.0	41.0	34.0	41.0
27	37.96106315177432	40.0	37.0	41.0	33.0	41.0
28	37.92344565845626	40.0	37.0	41.0	33.0	41.0
29	37.935885125471515	40.0	37.0	41.0	33.0	41.0
30	37.75832362823177	40.0	36.0	41.0	33.0	41.0
31	33.24373479638341	39.0	33.0	41.0	9.0	41.0
32	34.064699778914225	39.0	33.0	41.0	16.0	41.0
33	35.94546127476822	38.0	34.0	41.0	29.0	41.0
34	36.83992154313136	39.0	35.0	41.0	31.0	41.0
35	36.9787557876953	39.0	35.0	40.0	31.0	41.0
36	37.120917578137856	39.0	36.0	41.0	32.0	41.0
37	37.29337083342235	39.0	36.0	41.0	32.0	41.0
38	37.21488047568374	39.0	36.0	41.0	32.0	41.0
39	37.222704221416045	39.0	36.0	41.0	32.0	41.0
40	37.172094113598476	39.0	36.0	41.0	32.0	41.0
41	37.11113472028232	39.0	35.0	41.0	32.0	41.0
42	37.07632049526909	39.0	36.0	41.0	32.0	41.0
43	37.09834823694344	39.0	35.0	41.0	32.0	41.0
44	37.07786041629256	39.0	35.0	41.0	32.0	41.0
45	37.069342637913	39.0	35.0	41.0	32.0	41.0
46	36.98625734522697	39.0	35.0	41.0	32.0	41.0
47	36.84040136104306	39.0	35.0	41.0	31.0	41.0
48	36.865303391001945	39.0	35.0	41.0	31.0	41.0
49	36.86725672914308	39.0	35.0	41.0	31.0	41.0
50	36.69335131736288	39.0	35.0	41.0	31.0	41.0
51	36.03442217162931	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	14.0
12	8.0
13	19.0
14	26.0
15	33.0
16	83.0
17	173.0
18	305.0
19	589.0
20	1133.0
21	1945.0
22	3139.0
23	5118.0
24	6931.0
25	8663.0
26	10364.0
27	12049.0
28	14949.0
29	19419.0
30	26131.0
31	36417.0
32	53872.0
33	66214.0
34	109722.0
35	161822.0
36	208613.0
37	204454.0
38	329380.0
39	450018.0
40	296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.90506707346295	21.151828591258077	23.739727364113662	10.20337697116531
2	31.278122901518383	26.546806497115604	24.27514872334369	17.89992187802232
3	30.20537476896854	27.01848309406914	26.03910025191884	16.73704188504348
4	26.85386686467576	26.09037321288037	28.04498163007598	19.010778292367892
5	23.080800527972922	38.85866850818202	21.523268859124652	16.537262104720405
6	24.776214889136657	35.41500784972865	24.472272471905246	15.336504789229444
7	75.18937217760538	6.345086658810202	12.267922007359518	6.197619156224901
8	74.11269773723416	7.457097869573828	10.617140527753511	7.813063865438502
9	68.73111546982604	7.050667270240261	13.120508202807656	11.097709057126046
10	36.333706140110294	23.809188368659516	22.38607500287256	17.47103048835763
11	26.936781247491925	26.92973698934181	24.792670738093907	21.340811025072362
12	22.22879173073381	23.473258090648056	31.476459186318895	22.82149099229924
13	22.70964895921086	25.55206486260521	33.63205992007654	18.10622625810739
14	19.83357074023028	30.219982943656902	28.515330211148747	21.431116104964065
15	17.944727979042753	27.614242566142412	34.64446993978314	19.796559515031696
16	19.288160391984096	28.446215645528312	29.11975065635741	23.14587330613018
17	19.867868193846437	28.199493390811398	27.653332424893485	24.27930599044868
18	20.27580002852347	26.723779048182152	30.793685804145372	22.206735119149005
19	19.466576438573203	29.389511099614474	28.40793414426987	22.735978317542454
20	20.8486367916984	29.04792231915455	30.09422561373099	20.00921527541606
21	20.178219731198038	30.692121459177656	29.841844856565626	19.28781395305868
22	18.421139241310303	26.717312188241056	28.257059992251314	26.604488578197326
23	18.00362259636343	28.986313930251452	29.04838423772177	23.961679235663347
24	20.822307433366806	26.245693331108427	28.494601615444708	24.437397620080063
25	17.76215466534866	30.285806339485898	26.807386308849146	25.1446526863163
26	17.49349127868875	27.59293657222934	30.459718680044602	24.45385346903731
27	20.366509287161495	27.175131228177957	27.582889843392284	24.875469641268268
28	16.914360875035438	26.40314982270988	29.838784646057785	26.8437046561969
29	19.51600172526585	24.673438007929988	29.373979087791664	26.436581179012496
30	20.268409331447938	27.932677678420376	26.442066461998248	25.35684652813344
31	23.7779511255512	27.92234225047881	22.66368806177237	25.63601856219762
32	21.744412373181703	30.537147779875017	24.367243737683374	23.35119610925991
33	19.945008594572343	29.81222432844258	23.175724793536837	27.067042283448245
34	21.655839487917074	26.209605943044284	27.203481480241145	24.931073088797493
35	20.013545761983757	28.590276498680357	25.772630978453233	25.62354676088266
36	19.014704600189273	30.289963606590888	26.301874176846677	24.393457616373166
37	21.364946270209657	26.918881903012114	27.03222517144396	24.683946655334267
38	19.996974433384704	30.931337537177228	23.71443732255831	25.357250706879757
39	24.898565569629316	28.217392735291213	25.544905124813283	21.339136570266188
40	22.800877876237003	27.081304019211196	28.164618538986215	21.953199565565587
41	19.803776992644522	29.23967626437216	24.668125944406945	26.288420798576368
42	20.654630993465585	26.362096810048115	28.716842186098905	24.266430010387392
43	21.673450133292377	25.06277762027638	27.731223443291125	25.53254880314012
44	20.823924148352077	24.245470455399744	26.439179470953118	28.491425925295065
45	21.58655170283393	22.30096650686209	27.247479223768945	28.865002566535043
46	24.688334881722866	24.834936286994626	27.027836945055363	23.44889188622715
47	18.83218902631608	24.749712311342353	31.91562826410425	24.50247039823732
48	19.435339195464884	24.926107464199866	28.50378224696823	27.13477109336702
49	21.572001267966467	22.62719649496191	31.316231183314112	24.484571053757506
50	21.16181758027423	21.61126434618025	30.253356560138624	26.973561513406896
51	19.708794987259708	21.714734105237753	26.608645845302313	31.967825062200223
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1659.0
1	1953.0
2	2247.0
3	2686.0
4	3125.0
5	2721.0
6	2317.0
7	2406.0
8	2495.0
9	2860.5
10	3226.0
11	3600.5
12	3975.0
13	4075.5
14	4176.0
15	4079.0
16	3982.0
17	4300.5
18	4619.0
19	4598.0
20	4577.0
21	4600.5
22	4624.0
23	5473.5
24	6323.0
25	6645.5
26	9112.5
27	11257.0
28	12345.0
29	13433.0
30	16771.5
31	20110.0
32	22621.5
33	25133.0
34	30010.0
35	34887.0
36	40745.0
37	46603.0
38	50200.5
39	53798.0
40	60423.0
41	67048.0
42	81722.5
43	96397.0
44	115919.5
45	135442.0
46	181392.0
47	227342.0
48	242806.0
49	258270.0
50	247412.5
51	236555.0
52	194727.5
53	152900.0
54	126695.0
55	100490.0
56	83767.5
57	67045.0
58	56215.5
59	45386.0
60	38439.0
61	31492.0
62	25442.0
63	19392.0
64	16865.0
65	14338.0
66	11346.5
67	8355.0
68	6747.5
69	5140.0
70	4006.5
71	2873.0
72	2215.5
73	1558.0
74	1265.5
75	736.5
76	500.0
77	444.0
78	388.0
79	315.5
80	243.0
81	207.0
82	171.0
83	105.0
84	39.0
85	37.5
86	36.0
87	18.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1731907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.094551286891782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.0258778097156	17.07657878716714
2	14.475079273161501	7.8439155549699695
3	6.992208573526606	5.683522614121832
4	3.8296555777030963	4.150511978448309
5	2.3575618583131677	3.1938540341043007
6	1.5606871947712602	2.53716715449151
7	1.0848581616690551	2.057562157024175
8	0.7599630434389606	1.647268612527943
9	0.5894617632917145	1.437408177945212
>10	4.406035931410061	27.239464265182296
>50	0.8085718049507733	14.288590550978997
>100	0.0934024437641795	4.64469848184529
>500	0.009981938570398627	1.8353025315445712
>1k	0.005228634489256423	2.6168564007195636
>5k	9.506608162284406E-4	2.2923734191942455
>10k+	4.753304081142203E-4	1.4549252797346774
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	11809	0.6818495450390812	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	10785	0.6227239684347947	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	9935	0.5736451206675647	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	9564	0.5522236471126915	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	8860	0.5115748131972444	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	7240	0.4180363033349943	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3405	0.19660409017343308	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2986	0.17241110521523384	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	2735	0.15791841016867533	No Hit
TGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCTT	2677	0.15456950055632315	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	2671	0.15422306163090743	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	2189	0.12639246795584289	No Hit
GGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1908	0.11016757828220568	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	1895	0.10941696061047157	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	1874	0.10820442437151649	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1873	0.10814668455061385	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1866	0.1077425058042955	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	1836	0.10601031117721679	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	1741	0.10052502819146755	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6013024948799214	0.0
2	0.0	0.0	0.0	2.2456748543657366	0.0
3	0.0	0.0	0.0	3.1202599215777753	0.0
4	0.0	0.0	0.0	4.287528140945213	0.0
5	0.0	0.0	0.0	7.236185314800391	0.0
6	0.0	0.0	0.0	8.909831763483837	0.0
7	0.0	0.0	0.0	10.450272445344929	0.0
8	0.0	0.0	0.0	13.104860711343045	0.0
9	0.0	0.0	0.0	13.974422414136555	0.0
10	0.0	0.0	0.0	16.50105923701446	0.0
11	5.7739820902623523E-5	0.0	0.0	19.289430668043956	0.0
12	1.1547964180524705E-4	0.0	0.0	22.21274006052288	0.0
13	1.1547964180524705E-4	0.0	0.0	23.14235117705512	0.0
14	1.1547964180524705E-4	0.0	0.0	23.513791444921697	0.0
15	1.1547964180524705E-4	0.0	0.0	24.301651301137994	0.0
16	1.1547964180524705E-4	0.0	0.0	25.693123245070318	0.0
17	1.1547964180524705E-4	0.0	0.0	27.341479652198416	0.0
18	1.1547964180524705E-4	0.0	0.0	29.242794214700904	0.0
19	1.1547964180524705E-4	0.0	0.0	30.635536434693087	0.0
20	1.7321946270787056E-4	0.0	0.0	31.651353103832943	0.0
21	1.7321946270787056E-4	0.0	0.0	32.77023535328398	0.0
22	1.7321946270787056E-4	0.0	0.0	33.90643954900581	0.0
23	1.7321946270787056E-4	0.0	0.0	35.01221485911195	0.0
24	1.7321946270787056E-4	0.0	0.0	35.88171882208456	0.0
25	1.7321946270787056E-4	0.0	0.0	36.661668322837194	0.0
26	1.7321946270787056E-4	0.0	0.0	37.40911030442166	0.0
27	2.309592836104941E-4	0.0	0.0	38.10268103310397	0.0
28	2.309592836104941E-4	0.0	0.0	38.829625378268	0.0
29	2.309592836104941E-4	0.0	0.0	39.58688312940591	0.0
30	2.309592836104941E-4	0.0	0.0	40.38022826860796	0.0
31	2.309592836104941E-4	0.0	0.0	41.106941654488374	0.0
32	2.309592836104941E-4	0.0	0.0	41.87493901231417	0.0
33	2.309592836104941E-4	0.0	0.0	42.621110717838775	0.0
34	2.309592836104941E-4	0.0	0.0	43.37698271327502	0.0
35	2.309592836104941E-4	0.0	0.0	44.13262374942765	0.0
36	2.309592836104941E-4	0.0022518530152023175	0.0	44.84397834294798	0.0
37	2.309592836104941E-4	0.0022518530152023175	0.0	45.570229810261175	0.0
38	2.309592836104941E-4	0.0022518530152023175	0.0	46.27927481094539	0.0
39	2.309592836104941E-4	0.0022518530152023175	0.0	47.01701650261821	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	35	1.212411E-7	45.000004	1
ACCGCGT	20	7.034277E-4	45.000004	22
TAACGCG	20	7.034277E-4	45.000004	1
CCGTCGC	20	7.034277E-4	45.000004	19
CGTAGCG	20	7.034277E-4	45.000004	2
CGTCGTC	20	7.034277E-4	45.000004	16
CGGTCAT	20	7.034277E-4	45.000004	17
GCCGTAG	20	7.034277E-4	45.000004	1
GCGTACC	20	7.034277E-4	45.000004	13
TACGTCG	20	7.034277E-4	45.000004	14
CCCGTTT	25	3.8916274E-5	45.0	15
CCCGTAG	25	3.8916274E-5	45.0	44
TCCCGTA	25	3.8916274E-5	45.0	43
ATAATCG	115	0.0	43.04348	30
AACCGTA	55	6.184564E-11	40.909092	3
GATTACG	110	0.0	40.909092	1
CGTTTTT	1400	0.0	40.17857	1
TGCCGCG	130	0.0	39.80769	1
CGCTCGT	85	0.0	39.705883	11
ACGGTCC	245	0.0	39.489796	28
>>END_MODULE