SRR2935105.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935105.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1794065
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	6530	0.3639778937775387	Illumina PCR Primer Index 4 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	6412	0.357400651592891	TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	5416	0.30188426840722044	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	2716	0.15138804892799312	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	2441	0.1360597302773311	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2172	0.1210658476699562	TruSeq Adapter, Index 13 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	2100	0.11705261515051014	TruSeq Adapter, Index 13 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1804	0.10055377034834301	RNA PCR Primer, Index 13 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGAT	20	7.0343446E-4	45.000004	30
TATTCGC	30	2.1661854E-6	45.000004	24
CGTAATG	55	1.8189894E-12	45.000004	22
CGTAACA	20	7.0343446E-4	45.000004	32
CGCAATA	20	7.0343446E-4	45.000004	20
TCGACAG	55	1.8189894E-12	45.000004	1
ATTCGCG	25	3.891684E-5	45.0	1
CGACATA	25	3.891684E-5	45.0	29
GATATCG	25	3.891684E-5	45.0	24
CACGTCG	25	3.891684E-5	45.0	19
GTTGCGA	25	3.891684E-5	45.0	13
CGCAATC	80	0.0	42.187504	20
ATCGTAG	65	0.0	41.53846	1
TAGCGAG	125	0.0	41.399998	1
CACGTAA	60	3.6379788E-12	41.250004	20
TTAACGG	160	0.0	40.781254	2
TAGGTCG	50	1.0822987E-9	40.5	1
TATCGCG	50	1.0822987E-9	40.5	1
TATAACG	110	0.0	38.86364	1
CGACCGT	35	6.2502513E-6	38.571426	22