##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935105.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1794065
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00532366441573	33.0	31.0	34.0	30.0	34.0
2	32.326458071474555	34.0	31.0	34.0	30.0	34.0
3	32.385169991053836	34.0	31.0	34.0	30.0	34.0
4	35.90790913372704	37.0	35.0	37.0	35.0	37.0
5	30.410940517762736	37.0	35.0	37.0	0.0	37.0
6	32.87088371937472	37.0	35.0	37.0	17.0	37.0
7	35.069136291048544	37.0	35.0	37.0	32.0	37.0
8	35.62479564564271	37.0	35.0	37.0	35.0	37.0
9	37.48871194744895	39.0	37.0	39.0	35.0	39.0
10	37.39450298623517	39.0	37.0	39.0	35.0	39.0
11	37.25352202958087	39.0	37.0	39.0	34.0	39.0
12	37.12347545936184	39.0	37.0	39.0	34.0	39.0
13	37.08717465643664	39.0	37.0	39.0	33.0	39.0
14	38.25022560498087	40.0	38.0	41.0	33.0	41.0
15	38.35343591229972	40.0	38.0	41.0	34.0	41.0
16	38.43573727819226	40.0	38.0	41.0	34.0	41.0
17	38.411764902609434	40.0	37.0	41.0	34.0	41.0
18	38.375644695147614	40.0	37.0	41.0	34.0	41.0
19	38.369293754685586	40.0	37.0	41.0	34.0	41.0
20	38.29008425001324	40.0	37.0	41.0	34.0	41.0
21	38.24634447469852	40.0	37.0	41.0	34.0	41.0
22	38.243729184840014	40.0	37.0	41.0	34.0	41.0
23	38.16392995794467	40.0	37.0	41.0	34.0	41.0
24	38.13981767661707	40.0	37.0	41.0	34.0	41.0
25	38.087327939623144	40.0	37.0	41.0	34.0	41.0
26	38.023545969627634	40.0	36.0	41.0	34.0	41.0
27	37.98319570361163	40.0	36.0	41.0	34.0	41.0
28	37.92588953020097	40.0	36.0	41.0	34.0	41.0
29	37.90921120472224	40.0	36.0	41.0	34.0	41.0
30	37.78137024020869	40.0	36.0	41.0	33.0	41.0
31	33.221805230022326	39.0	33.0	41.0	9.0	41.0
32	34.0845259229738	38.0	33.0	41.0	16.0	41.0
33	36.02377450092388	38.0	34.0	41.0	30.0	41.0
34	36.88351982787692	38.0	35.0	41.0	31.0	41.0
35	37.02771025575996	39.0	35.0	41.0	32.0	41.0
36	37.203111927382785	39.0	35.0	41.0	32.0	41.0
37	37.3318246551825	39.0	35.0	41.0	33.0	41.0
38	37.27814042412064	39.0	35.0	41.0	33.0	41.0
39	37.27544208264472	39.0	35.0	41.0	33.0	41.0
40	37.22837968523994	39.0	35.0	41.0	33.0	41.0
41	37.17479689977788	39.0	35.0	41.0	33.0	41.0
42	37.10545604534953	39.0	35.0	41.0	32.0	41.0
43	37.10683949578193	39.0	35.0	41.0	32.0	41.0
44	37.05356662105331	39.0	35.0	41.0	32.0	41.0
45	37.01886943895567	39.0	35.0	41.0	32.0	41.0
46	36.95486562638477	39.0	35.0	41.0	32.0	41.0
47	36.85416916332463	39.0	35.0	41.0	32.0	41.0
48	36.8449587946925	39.0	35.0	41.0	32.0	41.0
49	36.84077499979098	39.0	35.0	41.0	32.0	41.0
50	36.661324422470756	39.0	35.0	41.0	32.0	41.0
51	36.04026721439859	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	16.0
11	8.0
12	14.0
13	30.0
14	40.0
15	49.0
16	75.0
17	171.0
18	303.0
19	607.0
20	1036.0
21	1656.0
22	2846.0
23	4369.0
24	6153.0
25	8116.0
26	10045.0
27	12509.0
28	15303.0
29	19423.0
30	26085.0
31	36629.0
32	56544.0
33	66449.0
34	120557.0
35	173444.0
36	212220.0
37	209848.0
38	317167.0
39	491924.0
40	417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.890789910064576	21.570623137957654	25.03231488268262	11.506272069295148
2	31.871977882629672	24.59386922993314	25.29702101094442	18.237131876492768
3	32.17469824114511	24.76052985817125	25.593219866615758	17.471552034067887
4	28.548965617187783	25.47588855476251	26.380649530535404	19.594496297514304
5	22.424103920426518	39.31206505895829	20.557783580862456	17.70604743975274
6	26.860620991993045	34.04659251476396	23.376912207751673	15.71587428549133
7	79.29656952228599	6.2905747562100585	8.94220666475295	5.47064905675101
8	79.55620337055792	5.6181910911812	8.605262351141125	6.220343187119753
9	73.50725865562285	7.318965589318113	11.015654393792868	8.158121361266176
10	42.312346542628056	23.702652913913376	18.17269719881944	15.812303344639131
11	30.307876247516113	25.989415099229962	24.139370647105874	19.563338006148047
12	27.846036793538698	22.584020088458335	28.852354847789798	20.717588270213174
13	25.317756045628226	23.75131335821166	30.456588808097813	20.474341788062304
14	20.53426157915126	28.103218110826532	28.89343474177357	22.46908556824864
15	19.27811980056464	26.46593072157363	33.50413725255216	20.751812225309564
16	21.889786601934713	25.634021063896796	30.42849618046169	22.047696153706806
17	21.476423652431766	26.037127974738933	27.975630760312477	24.510817612516828
18	21.731431135438235	26.039357548360847	29.54469319673479	22.68451811946613
19	23.074972200003902	27.089263766920375	27.726810344106816	22.108953688968906
20	25.264859411448302	26.770323260305506	27.935442695777468	20.029374632468723
21	23.991884352016232	27.33680217829343	28.09123415260874	20.5800793170816
22	21.93761095612478	25.0928478065176	27.514164759916724	25.455376477440893
23	21.55590795205302	26.664864427988956	28.136160061090315	23.64306755886771
24	22.338878468728836	24.75423131268934	28.841597155064058	24.065293063517764
25	21.18407081125823	27.30670293439758	26.463812626632816	25.045413627711373
26	20.842723089743124	27.152026264377266	27.363557061756406	24.641693584123207
27	22.020997009584377	26.60600368437041	27.793641813423704	23.579357492621504
28	19.181189087351907	26.990995309534494	28.192401055703108	25.63541454741049
29	20.738657740940265	25.60275129384944	27.1824599443164	26.476131020893888
30	21.469344756182192	26.763467321418123	27.563215379598844	24.203972542800848
31	24.568173393940576	27.416119259893033	23.480921817213982	24.53478552895241
32	21.534113869898803	28.444844529044378	25.102769409135124	24.918272191921698
33	22.046581366895847	27.642699679220094	25.62209284501955	24.688626108864504
34	21.586731807375987	25.531404937948178	27.062118708073562	25.81974454660227
35	20.782468862610887	25.23604217238506	28.126349937153893	25.85513902785016
36	20.83893281458587	26.387895644806626	27.15330826920987	25.619863271397637
37	21.39783118225928	26.813967163954484	28.169380708056842	23.61882094572939
38	20.600256958359925	28.011248198922555	25.962214301042604	25.426280541674913
39	22.914052723842225	26.3377859776541	25.688924314336436	25.05923698416724
40	23.269056583791556	24.664769671110022	28.866010986224023	23.2001627588744
41	20.591059967169528	24.781432110876697	28.1718889783815	26.45561894357228
42	21.453347565444954	23.425293955347215	28.724433061232453	26.396925417975382
43	21.49465041679092	23.4295858845694	28.884293489923717	26.191470208715963
44	20.857103839604473	24.671960046040695	27.024606131884855	27.446329982469976
45	21.315448436929543	23.568209624511933	27.06724672740397	28.049095211154558
46	22.54890430391318	25.09067397223623	27.422083369331656	24.938338354518926
47	20.079372820940154	23.798970494380082	30.90484458478372	25.216812099896046
48	19.453475765928214	23.244865709993785	29.82818348276122	27.473475041316785
49	21.537736927034416	22.297129702658488	30.68690376324158	25.478229607065515
50	20.11710835449106	22.640595519114413	30.065242898111272	27.177053228283256
51	19.674426511859938	22.24495767990569	27.85211238165841	30.22850342657596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1027.0
1	1701.5
2	2376.0
3	2977.5
4	3579.0
5	3010.0
6	2441.0
7	2536.0
8	2631.0
9	2723.0
10	2815.0
11	2974.0
12	3133.0
13	3390.0
14	3647.0
15	3843.0
16	4039.0
17	4113.5
18	4188.0
19	4538.0
20	4888.0
21	4828.0
22	4768.0
23	5145.5
24	5523.0
25	7785.5
26	11870.5
27	13693.0
28	16917.0
29	20141.0
30	22408.5
31	24676.0
32	28827.0
33	32978.0
34	34119.0
35	35260.0
36	39103.0
37	42946.0
38	48913.5
39	54881.0
40	61916.5
41	68952.0
42	79442.0
43	89932.0
44	105641.5
45	121351.0
46	147271.5
47	173192.0
48	190131.5
49	207071.0
50	202672.5
51	198274.0
52	170698.0
53	143122.0
54	126626.5
55	110131.0
56	97768.5
57	85406.0
58	82144.5
59	78883.0
60	73686.0
61	68489.0
62	63188.5
63	57888.0
64	48570.5
65	39253.0
66	31646.0
67	24039.0
68	20403.5
69	16768.0
70	14154.5
71	11541.0
72	10066.0
73	8591.0
74	7036.0
75	4274.5
76	3068.0
77	2231.5
78	1395.0
79	1127.5
80	860.0
81	696.5
82	533.0
83	302.0
84	71.0
85	80.5
86	90.0
87	47.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1794065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.59537922788189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.38338630408079	16.32724560579848
2	13.466580356436433	6.6243130153859795
3	6.091259602266186	4.494505196796415
4	3.2329548900392697	3.180630061886042
5	1.9372589366437643	2.38238091046783
6	1.2610357657055937	1.8609391726470896
7	0.8702073084813728	1.4982155131281636
8	0.6404966809017846	1.2602607008783235
9	0.49083564980747213	1.086506005102075
>10	3.765844025991353	21.95956244897177
>50	1.6384413614387028	28.047130327103503
>100	0.21070366056497547	7.635078336626075
>500	0.00715983288337429	1.2128805192642773
>1k	0.0030684998071604094	1.275772949612438
>5k	7.671249517901023E-4	1.1545792363315324
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	6530	0.3639778937775387	Illumina PCR Primer Index 4 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	6412	0.357400651592891	TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	5416	0.30188426840722044	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	2716	0.15138804892799312	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	2441	0.1360597302773311	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2172	0.1210658476699562	TruSeq Adapter, Index 13 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	2100	0.11705261515051014	TruSeq Adapter, Index 13 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1804	0.10055377034834301	RNA PCR Primer, Index 13 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2295736219144792E-4	0.0	0.0	0.38471292846134336	0.0
2	2.2295736219144792E-4	0.0	0.0	1.3210781103248768	0.0
3	2.2295736219144792E-4	0.0	0.0	1.8586283105684576	0.0
4	2.2295736219144792E-4	0.0	0.0	2.582124950879706	0.0
5	2.2295736219144792E-4	0.0	0.0	4.383286001343318	0.0
6	2.2295736219144792E-4	0.0	0.0	5.535473909808173	0.0
7	2.2295736219144792E-4	0.0	0.0	6.5395066510968105	0.0
8	2.2295736219144792E-4	0.0	0.0	8.285485754418039	0.0
9	2.2295736219144792E-4	0.0	0.0	8.880837650809752	0.0
10	2.2295736219144792E-4	0.0	0.0	10.280954146031497	0.0
11	2.2295736219144792E-4	0.0	0.0	12.2831112585107	0.0
12	2.2295736219144792E-4	0.0	0.0	14.048320434320942	0.0
13	2.2295736219144792E-4	0.0	0.0	14.692277035670392	0.0
14	2.2295736219144792E-4	0.0	0.0	14.955812637780683	0.0
15	2.2295736219144792E-4	0.0	0.0	15.476139381794974	0.0
16	2.2295736219144792E-4	0.0	0.0	16.454643505112692	0.0
17	2.2295736219144792E-4	0.0	0.0	17.601982090949882	0.0
18	2.2295736219144792E-4	0.0	0.0	18.865202765786076	0.0
19	3.344360432871719E-4	0.0	0.0	19.705250367182906	0.0
20	3.344360432871719E-4	0.0	0.0	20.41013006775117	0.0
21	3.344360432871719E-4	0.0	0.0	21.215842235370513	0.0
22	3.344360432871719E-4	0.0	0.0	22.062076903568155	0.0
23	3.344360432871719E-4	5.573934054786198E-5	0.0	22.834289727518232	0.0
24	3.344360432871719E-4	5.573934054786198E-5	0.0	23.455950592648538	0.0
25	3.344360432871719E-4	5.573934054786198E-5	0.0	24.03391181478932	0.0
26	3.344360432871719E-4	5.573934054786198E-5	0.0	24.540303723666646	0.0
27	3.344360432871719E-4	5.573934054786198E-5	0.0	25.032091925320433	0.0
28	3.9017538383503384E-4	5.573934054786198E-5	0.0	25.55280884471856	0.0
29	3.9017538383503384E-4	5.573934054786198E-5	0.0	26.10808415525636	0.0
30	3.9017538383503384E-4	5.573934054786198E-5	0.0	26.724895697759	0.0
31	5.573934054786198E-4	5.573934054786198E-5	0.0	27.28329241136748	0.0
32	5.573934054786198E-4	5.573934054786198E-5	0.0	27.903448314302995	0.0
33	5.573934054786198E-4	5.573934054786198E-5	0.0	28.480071792270625	0.0
34	5.573934054786198E-4	5.573934054786198E-5	0.0	29.053183691783744	0.0
35	5.573934054786198E-4	5.573934054786198E-5	0.0	29.673060898016516	0.0
36	5.573934054786198E-4	5.573934054786198E-5	0.0	30.2717571548411	0.0
37	5.573934054786198E-4	5.573934054786198E-5	0.0	30.91342844322809	0.0
38	5.573934054786198E-4	5.573934054786198E-5	0.0	31.503819538311042	0.0
39	6.688720865743438E-4	5.573934054786198E-5	0.0	32.13016250804737	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGAT	20	7.0343446E-4	45.000004	30
TATTCGC	30	2.1661854E-6	45.000004	24
CGTAATG	55	1.8189894E-12	45.000004	22
CGTAACA	20	7.0343446E-4	45.000004	32
CGCAATA	20	7.0343446E-4	45.000004	20
TCGACAG	55	1.8189894E-12	45.000004	1
ATTCGCG	25	3.891684E-5	45.0	1
CGACATA	25	3.891684E-5	45.0	29
GATATCG	25	3.891684E-5	45.0	24
CACGTCG	25	3.891684E-5	45.0	19
GTTGCGA	25	3.891684E-5	45.0	13
CGCAATC	80	0.0	42.187504	20
ATCGTAG	65	0.0	41.53846	1
TAGCGAG	125	0.0	41.399998	1
CACGTAA	60	3.6379788E-12	41.250004	20
TTAACGG	160	0.0	40.781254	2
TAGGTCG	50	1.0822987E-9	40.5	1
TATCGCG	50	1.0822987E-9	40.5	1
TATAACG	110	0.0	38.86364	1
CGACCGT	35	6.2502513E-6	38.571426	22
>>END_MODULE