SRR2935104.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935104.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502258
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	8230	0.5478419818699584	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	7959	0.5298024706808019	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	7753	0.5160897795185647	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	2609	0.17367189923435256	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	2282	0.15190466617584997	No Hit
ACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	1974	0.13140219589444688	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	1737	0.11562594441167895	No Hit
GCCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	1652	0.10996779514570734	TruSeq Adapter, Index 15 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.0339785E-4	45.000004	12
TATCACG	40	6.8175723E-9	45.000004	1
GTCGACT	20	7.0339785E-4	45.000004	30
CGACGAA	30	2.165949E-6	45.000004	16
ACGTTAT	20	7.0339785E-4	45.000004	25
TACGGAT	20	7.0339785E-4	45.000004	4
ATCGTAC	40	6.8175723E-9	45.000004	11
ATCGTAA	20	7.0339785E-4	45.000004	14
GCGATCG	20	7.0339785E-4	45.000004	9
TCGAGTA	20	7.0339785E-4	45.000004	26
ACCGTAT	20	7.0339785E-4	45.000004	34
CGCCGAT	30	2.165949E-6	45.000004	28
TACCGGC	20	7.0339785E-4	45.000004	37
CCCGATA	20	7.0339785E-4	45.000004	27
ATTCACG	45	3.8562575E-10	45.0	1
GTTCGAC	25	3.8913797E-5	45.0	26
TACGACT	45	3.8562575E-10	45.0	42
CTACCGT	50	2.1827873E-11	45.0	9
TCATCGA	25	3.8913797E-5	45.0	16
ATACCGG	25	3.8913797E-5	45.0	2