##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935104.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502258
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.892890568730536	33.0	31.0	34.0	30.0	34.0
2	32.210175615639926	34.0	31.0	34.0	30.0	34.0
3	32.27026116685683	34.0	31.0	34.0	30.0	34.0
4	35.82811541026908	37.0	35.0	37.0	35.0	37.0
5	30.329326919876614	37.0	35.0	37.0	0.0	37.0
6	32.80015416792588	37.0	35.0	37.0	17.0	37.0
7	35.000640369363985	37.0	35.0	37.0	32.0	37.0
8	35.54162134600049	37.0	35.0	37.0	33.0	37.0
9	37.339325868126515	39.0	37.0	39.0	34.0	39.0
10	37.322547125726736	39.0	37.0	39.0	34.0	39.0
11	37.26637634813727	39.0	37.0	39.0	34.0	39.0
12	37.1388050521282	39.0	37.0	39.0	34.0	39.0
13	37.08271016030535	39.0	37.0	39.0	33.0	39.0
14	38.24950707534924	40.0	37.0	41.0	34.0	41.0
15	38.346286057388276	40.0	38.0	41.0	34.0	41.0
16	38.42484113913855	40.0	38.0	41.0	34.0	41.0
17	38.41353615690514	40.0	38.0	41.0	34.0	41.0
18	38.36327315281397	40.0	37.0	41.0	34.0	41.0
19	38.36365857262867	40.0	37.0	41.0	34.0	41.0
20	38.28935309380945	40.0	37.0	41.0	34.0	41.0
21	38.22820713885365	40.0	37.0	41.0	34.0	41.0
22	38.206744114526266	40.0	37.0	41.0	34.0	41.0
23	38.146885554944625	40.0	37.0	41.0	34.0	41.0
24	38.13057810309547	40.0	37.0	41.0	34.0	41.0
25	38.07307599626695	40.0	37.0	41.0	34.0	41.0
26	37.97579710009865	40.0	36.0	41.0	34.0	41.0
27	37.93648361333406	40.0	36.0	41.0	34.0	41.0
28	37.83350929068109	40.0	36.0	41.0	33.0	41.0
29	37.851528166267045	40.0	36.0	41.0	33.0	41.0
30	37.704803036495726	40.0	36.0	41.0	33.0	41.0
31	33.08328263187815	38.0	33.0	41.0	9.0	41.0
32	33.96152525065601	38.0	33.0	41.0	16.0	41.0
33	35.85562400067099	38.0	34.0	40.0	29.0	41.0
34	36.679582335391125	38.0	35.0	40.0	31.0	41.0
35	36.835698661614714	39.0	35.0	40.0	31.0	41.0
36	36.97328488182456	39.0	35.0	40.0	31.0	41.0
37	37.041607367043476	39.0	35.0	41.0	32.0	41.0
38	36.97831264669584	39.0	35.0	41.0	32.0	41.0
39	37.04484515975285	39.0	35.0	41.0	32.0	41.0
40	36.98801204586695	39.0	35.0	41.0	32.0	41.0
41	36.897334545730494	39.0	35.0	41.0	31.0	41.0
42	36.80019810179077	39.0	35.0	41.0	31.0	41.0
43	36.76424222736707	39.0	35.0	41.0	31.0	41.0
44	36.68392579703353	39.0	35.0	41.0	31.0	41.0
45	36.69297883585909	39.0	35.0	41.0	31.0	41.0
46	36.605336100723044	39.0	35.0	41.0	31.0	41.0
47	36.48311674825496	39.0	35.0	40.0	31.0	41.0
48	36.451666091976215	39.0	35.0	40.0	31.0	41.0
49	36.415903260292176	39.0	35.0	40.0	31.0	41.0
50	36.19715787834047	38.0	35.0	40.0	31.0	41.0
51	35.434957244361485	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	12.0
12	20.0
13	19.0
14	27.0
15	60.0
16	86.0
17	168.0
18	328.0
19	600.0
20	948.0
21	1714.0
22	2645.0
23	4382.0
24	6103.0
25	7812.0
26	9472.0
27	11563.0
28	14271.0
29	18246.0
30	23754.0
31	33087.0
32	49045.0
33	58794.0
34	102021.0
35	148125.0
36	176682.0
37	181401.0
38	279682.0
39	370855.0
40	322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.259318971841054	21.58544005091003	24.573608527962573	11.581632449286342
2	31.548775243666533	25.98834554384134	24.24090935112344	18.221969861368688
3	31.438208350363254	24.884806737591013	26.090258797090783	17.586726114954953
4	28.88139054676361	26.393202765437096	25.612245033809106	19.113161653990193
5	21.49178103894271	39.08376590439192	20.970033110158177	18.45441994650719
6	25.60745224854852	33.666054698993115	24.279983864289623	16.446509188168744
7	76.58398224539327	7.203955645435071	9.917936865704826	6.294125243466834
8	75.2406044767277	6.671557082738118	10.384634330454556	7.703204110079627
9	68.05428894371008	9.297470873844572	13.540949690399383	9.107290492045973
10	36.73723155410056	27.805343689299704	19.57593169748472	15.881493059115012
11	29.24903711612786	26.14078274171281	23.82167377374592	20.788506368413415
12	27.175292126918276	22.58340444850352	30.253125628220985	19.988177796357217
13	23.44670489356688	25.202794726338617	30.503282392238884	20.847217987855615
14	18.35869737421934	29.424905708606648	29.32419065167235	22.892206265501667
15	16.83066424009724	27.610969620398095	35.3022583337882	20.25610780571646
16	18.933165940870346	27.274143322917897	31.957293620669684	21.835397115542072
17	18.286139930691	26.68403163770804	28.23942358769266	26.790404843908306
18	19.26340215861723	27.602582246192064	30.611120060602108	22.5228955345886
19	21.74573209129191	28.095240631103312	28.389131560624076	21.769895716980706
20	24.294095954223575	27.85466943760659	27.62721183711453	20.224022771055306
21	22.44481307471819	28.07660202175658	28.24068835046976	21.237896553055467
22	19.482006419669588	27.55718391914039	26.732026056775865	26.228783604414158
23	20.983346402548698	27.820720542010758	28.013962981059176	23.181970074381365
24	22.606569577263027	25.071192831058315	27.92176843125482	24.400469160423842
25	21.03520167640978	27.945000126476277	25.705105248232996	25.31469294888095
26	20.747634560774515	28.91134545464228	26.7047338073753	23.63628617720791
27	24.12315327992928	28.42660847870339	25.762685237821998	21.68755300354533
28	19.50676914351596	29.33603948189991	27.694244264300806	23.46294711028332
29	23.077593862039677	26.73402305063444	26.54923455225401	23.63914853507187
30	25.25238674049331	28.181311066408032	24.552240693675785	22.01406149942287
31	25.324544785249937	30.552142175312095	22.620548534273073	21.502764505164894
32	25.34305026167276	28.71124667001274	25.335794517319925	20.609908550994568
33	25.030387589881364	28.96752754853028	24.813913455611488	21.18817140597687
34	24.391083289288524	26.901703968293063	26.633441126624056	22.073771615794357
35	24.55596841554513	24.646565370262632	27.614897041653297	23.18256917253894
36	27.15359146032173	28.12606090298737	24.934132485897894	19.78621515079301
37	25.021134851669952	27.825380194347442	28.046048015720338	19.10743693826227
38	20.743241174285643	28.624310870702637	28.269911027266954	22.36253692774477
39	25.822927885889108	24.810651698975807	28.031403394090766	21.335017021044322
40	25.86752741539736	23.342128981839338	30.589685659853366	20.200657942909938
41	23.819410514039532	24.895590504427332	27.292848498726585	23.992150482806547
42	26.356191812591444	23.701721009307324	27.92562928604807	22.01645789205316
43	26.09411965188403	24.267203103594724	28.155816111480185	21.482861133041062
44	23.098562297554746	25.13669422961968	26.629314005982994	25.13542946684258
45	23.42973044576897	22.905785823739997	27.130692597410032	26.533791133081003
46	25.045032211510936	25.405889001756023	27.41879224474092	22.130286541992124
47	22.56709566532513	23.887441438155097	31.497718767348886	22.047744129170887
48	21.668581561888836	22.42510940198022	31.104444110132878	24.801864925998064
49	23.749715428375154	21.73860947986298	31.879943391880754	22.631731699881115
50	21.643752271580514	21.64621523067276	31.158762343086206	25.551270154660514
51	21.189902133987637	21.268783391401477	27.987070130430325	29.554244344180557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1643.0
1	1943.5
2	2244.0
3	2895.0
4	3546.0
5	3050.5
6	2555.0
7	2543.0
8	2531.0
9	2830.5
10	3130.0
11	3298.0
12	3466.0
13	3467.5
14	3469.0
15	3689.5
16	3910.0
17	4023.5
18	4137.0
19	4143.0
20	4149.0
21	4514.0
22	4879.0
23	5221.5
24	5564.0
25	7439.0
26	11887.5
27	14461.0
28	15633.0
29	16805.0
30	19577.0
31	22349.0
32	23914.5
33	25480.0
34	29267.0
35	33054.0
36	35406.0
37	37758.0
38	43247.5
39	48737.0
40	55618.5
41	62500.0
42	71584.0
43	80668.0
44	93301.5
45	105935.0
46	127538.0
47	149141.0
48	167933.5
49	186726.0
50	181788.0
51	176850.0
52	147371.5
53	117893.0
54	100869.0
55	83845.0
56	73448.5
57	63052.0
58	58316.0
59	53580.0
60	53689.5
61	53799.0
62	48238.5
63	42678.0
64	34788.0
65	26898.0
66	21323.0
67	15748.0
68	13189.0
69	10630.0
70	9212.5
71	7795.0
72	6517.0
73	5239.0
74	3921.0
75	2189.0
76	1775.0
77	1363.5
78	952.0
79	662.0
80	372.0
81	267.0
82	162.0
83	136.0
84	110.0
85	89.5
86	69.0
87	55.5
88	42.0
89	28.0
90	14.0
91	7.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1502258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.309594733612002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.79076766982611	17.046188398727356
2	13.70641094492308	7.212202344265403
3	6.419996591103523	5.0672252550931285
4	3.6227118983472026	3.812483275285964
5	2.261834231964193	2.9753970998794235
6	1.4032826552065276	2.2151878773114433
7	0.9905331043724772	1.8242367182386532
8	0.7276585326190196	1.5315520878128954
9	0.5574152600989197	1.3198832632380153
>10	4.248546239254091	26.00004650584516
>50	1.1208517973958787	19.76681805455324
>100	0.1349636137384994	6.03262009219077
>500	0.009923795099354992	1.7402835929986422
>1k	0.004253055042580711	1.6698636130684816
>5k	8.506110085161421E-4	1.7860118214914265
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	8230	0.5478419818699584	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	7959	0.5298024706808019	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	7753	0.5160897795185647	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	2609	0.17367189923435256	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	2282	0.15190466617584997	No Hit
ACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	1974	0.13140219589444688	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	1737	0.11562594441167895	No Hit
GCCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	1652	0.10996779514570734	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.656646195260734E-5	0.0	0.0	0.5807923805364991	0.0
2	6.656646195260734E-5	0.0	0.0	2.013302641756609	0.0
3	6.656646195260734E-5	0.0	0.0	2.777552191434494	0.0
4	6.656646195260734E-5	0.0	0.0	3.686450662935395	0.0
5	6.656646195260734E-5	0.0	0.0	5.94245462497121	0.0
6	6.656646195260734E-5	0.0	0.0	7.481271525929634	0.0
7	1.3313292390521467E-4	0.0	0.0	8.787704908211506	0.0
8	1.9969938585782203E-4	0.0	0.0	10.983532788642163	0.0
9	1.9969938585782203E-4	0.0	0.0	11.800236710338703	0.0
10	1.9969938585782203E-4	0.0	0.0	13.525306571840522	0.0
11	1.9969938585782203E-4	0.0	0.0	16.128854031730903	0.0
12	2.6626584781042935E-4	0.0	0.0	18.237280147617785	0.0
13	2.6626584781042935E-4	0.0	0.0	19.096120639730326	0.0
14	2.6626584781042935E-4	0.0	0.0	19.444862333900037	0.0
15	2.6626584781042935E-4	0.0	0.0	20.102405845067892	0.0
16	2.6626584781042935E-4	0.0	0.0	21.380615047481857	0.0
17	2.6626584781042935E-4	0.0	0.0	22.818716891505986	0.0
18	3.328323097630367E-4	0.0	0.0	24.547780740724964	0.0
19	3.328323097630367E-4	0.0	0.0	25.516522461521255	0.0
20	3.328323097630367E-4	0.0	0.0	26.42009561606595	0.0
21	3.9939877171564405E-4	0.0	0.0	27.404480455421105	0.0
22	3.9939877171564405E-4	0.0	0.0	28.477598388559088	0.0
23	3.9939877171564405E-4	0.0	0.0	29.425438240302267	0.0
24	3.9939877171564405E-4	0.0	0.0	30.162595239965437	0.0
25	3.9939877171564405E-4	0.0	0.0	30.853555115033505	0.0
26	3.9939877171564405E-4	0.0	0.0	31.45192104152549	0.0
27	3.9939877171564405E-4	0.0	0.0	32.03104926051317	0.0
28	4.659652336682514E-4	0.0	0.0	32.625421199288006	0.0
29	4.659652336682514E-4	0.0	0.0	33.247218520387314	0.0
30	4.659652336682514E-4	0.0	0.0	33.92759432800491	0.0
31	4.659652336682514E-4	0.0	0.0	34.58327397823809	0.0
32	4.659652336682514E-4	0.0	0.0	35.32602256070528	0.0
33	4.659652336682514E-4	0.0	0.0	36.000540519671056	0.0
34	4.659652336682514E-4	0.0	0.0	36.654289742507615	0.0
35	4.659652336682514E-4	0.0	0.0	37.33333422088616	0.0
36	4.659652336682514E-4	0.0	0.0	38.06043968479449	0.0
37	5.99098157573466E-4	0.0	0.0	38.7590547029871	0.0
38	5.99098157573466E-4	0.0	0.0	39.461397443049066	0.0
39	5.99098157573466E-4	0.0	0.0	40.13172171491182	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.0339785E-4	45.000004	12
TATCACG	40	6.8175723E-9	45.000004	1
GTCGACT	20	7.0339785E-4	45.000004	30
CGACGAA	30	2.165949E-6	45.000004	16
ACGTTAT	20	7.0339785E-4	45.000004	25
TACGGAT	20	7.0339785E-4	45.000004	4
ATCGTAC	40	6.8175723E-9	45.000004	11
ATCGTAA	20	7.0339785E-4	45.000004	14
GCGATCG	20	7.0339785E-4	45.000004	9
TCGAGTA	20	7.0339785E-4	45.000004	26
ACCGTAT	20	7.0339785E-4	45.000004	34
CGCCGAT	30	2.165949E-6	45.000004	28
TACCGGC	20	7.0339785E-4	45.000004	37
CCCGATA	20	7.0339785E-4	45.000004	27
ATTCACG	45	3.8562575E-10	45.0	1
GTTCGAC	25	3.8913797E-5	45.0	26
TACGACT	45	3.8562575E-10	45.0	42
CTACCGT	50	2.1827873E-11	45.0	9
TCATCGA	25	3.8913797E-5	45.0	16
ATACCGG	25	3.8913797E-5	45.0	2
>>END_MODULE