##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935103.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1414579
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.775162786949334	33.0	31.0	34.0	30.0	34.0
2	32.09462956823196	33.0	31.0	34.0	30.0	34.0
3	32.16298700885564	34.0	31.0	34.0	30.0	34.0
4	35.73319835795668	37.0	35.0	37.0	33.0	37.0
5	30.26662066947127	37.0	35.0	37.0	0.0	37.0
6	32.72784552859897	37.0	35.0	37.0	17.0	37.0
7	34.946031292702635	37.0	35.0	37.0	32.0	37.0
8	35.511948077838	37.0	35.0	37.0	33.0	37.0
9	37.340169053831566	39.0	37.0	39.0	34.0	39.0
10	37.25159429059812	39.0	37.0	39.0	34.0	39.0
11	37.169661786298256	39.0	37.0	39.0	34.0	39.0
12	37.036153512811936	39.0	37.0	39.0	33.0	39.0
13	36.95586602091506	39.0	37.0	39.0	33.0	39.0
14	37.97930550361627	40.0	37.0	41.0	33.0	41.0
15	38.12342824260787	40.0	37.0	41.0	33.0	41.0
16	38.22640163610516	40.0	37.0	41.0	33.0	41.0
17	38.23407388346639	40.0	37.0	41.0	34.0	41.0
18	38.189134717820636	40.0	37.0	41.0	34.0	41.0
19	38.1853597430755	40.0	37.0	41.0	34.0	41.0
20	38.095991810991116	40.0	37.0	41.0	34.0	41.0
21	38.035624733577976	40.0	37.0	41.0	34.0	41.0
22	38.03635852080372	40.0	37.0	41.0	34.0	41.0
23	37.976011237265645	40.0	36.0	41.0	34.0	41.0
24	37.94765792507877	40.0	36.0	41.0	33.0	41.0
25	37.880094360230146	40.0	36.0	41.0	33.0	41.0
26	37.83983079064513	40.0	36.0	41.0	33.0	41.0
27	37.820902897611234	40.0	36.0	41.0	33.0	41.0
28	37.78565141996311	40.0	36.0	41.0	33.0	41.0
29	37.80057882946092	40.0	36.0	41.0	33.0	41.0
30	37.64545988594487	40.0	36.0	41.0	33.0	41.0
31	33.10953647693059	38.0	33.0	40.0	9.0	41.0
32	34.01811280953556	38.0	33.0	40.0	16.0	41.0
33	35.926409200193135	38.0	34.0	40.0	29.0	41.0
34	36.808386806251185	38.0	35.0	40.0	31.0	41.0
35	36.987349593059136	39.0	35.0	40.0	32.0	41.0
36	37.1416202276437	39.0	35.0	41.0	32.0	41.0
37	37.25309296971042	39.0	35.0	41.0	32.0	41.0
38	37.200444089725636	39.0	35.0	41.0	32.0	41.0
39	37.19412560203425	39.0	35.0	41.0	32.0	41.0
40	37.15097636823394	39.0	35.0	41.0	32.0	41.0
41	37.07930840200512	39.0	35.0	41.0	32.0	41.0
42	37.041232762539245	39.0	35.0	41.0	32.0	41.0
43	37.03882073747737	39.0	35.0	41.0	32.0	41.0
44	36.945947168733596	39.0	35.0	41.0	32.0	41.0
45	36.925386987930686	39.0	35.0	41.0	32.0	41.0
46	36.862547796906355	39.0	35.0	41.0	32.0	41.0
47	36.782712029515494	39.0	35.0	40.0	31.0	41.0
48	36.81149373771277	39.0	35.0	41.0	32.0	41.0
49	36.81220066182235	39.0	35.0	41.0	32.0	41.0
50	36.6182150307618	39.0	35.0	40.0	31.0	41.0
51	36.01127331877541	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	6.0
10	19.0
11	20.0
12	15.0
13	18.0
14	27.0
15	37.0
16	81.0
17	143.0
18	294.0
19	502.0
20	855.0
21	1427.0
22	2363.0
23	3732.0
24	5222.0
25	6764.0
26	8293.0
27	10076.0
28	12732.0
29	16768.0
30	22623.0
31	32253.0
32	46119.0
33	57854.0
34	100258.0
35	132582.0
36	171307.0
37	167864.0
38	262133.0
39	351965.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57853396664308	21.63746245349323	24.234843016897607	11.549160562966083
2	32.46937781488344	23.861799164274316	24.482619917304017	19.186203103538226
3	31.766907327197703	24.373117372730686	26.41563320252881	17.444342097542805
4	28.22238984178331	25.223052229673986	26.066412692398234	20.488145236144465
5	21.73784567705303	37.952139823933486	21.69444053672506	18.615573962288426
6	25.220224533235687	31.976722402919876	26.291426636476295	16.511626427368142
7	75.60482659505054	6.195341511502716	11.310361598751289	6.889470294695453
8	73.63491187130587	5.266867385985512	11.959530008574989	9.138690734133618
9	66.84794557249896	7.438538250603183	15.463752819743542	10.24976335715432
10	37.069191611072974	23.6585584827712	21.77312119012088	17.49912871603495
11	26.50661433543125	23.807295315426003	27.26146789963657	22.424622449506177
12	23.792449909124905	20.45576811192588	33.565675724014	22.186106254935215
13	22.50040471405273	23.674464275236662	34.59516930478962	19.229961705920985
14	17.970788481944098	27.0638119185991	32.54303930710126	22.422360292355535
15	16.542943165422365	25.906223689168296	35.95147390142226	21.599359243987077
16	19.205714208962526	26.889484433177646	30.661631481875528	23.243169875984304
17	19.323487765617898	26.789101209617844	27.53391645146718	26.35349457329707
18	20.075513633384915	25.0661150773481	31.98789180385118	22.8704794854158
19	19.82695911645797	26.938827736026056	28.352110415890525	24.88210273162545
20	20.8397692882476	28.132257017812368	29.481421680938286	21.546552013001747
21	20.830932736877898	28.31909705997332	30.6186504960133	20.23131970713548
22	19.550975944079475	24.482619917304017	29.383512691762004	26.582891446854507
23	18.717865880944082	26.279055464558716	29.909959076163297	25.093119578333905
24	21.425809375086153	24.33162092749857	29.190522409847734	25.052047287567536
25	19.316842678987882	26.900512449287035	26.412734813679545	27.369910058045537
26	18.412545358018182	25.87695703103185	27.65176070053352	28.058736910416453
27	21.312277363088242	24.50997788034461	27.54642900820668	26.631315748360468
28	17.519488130390737	24.084975105667482	29.492096234992886	28.9034405289489
29	19.496542787642117	22.82926580982752	27.899325523707052	29.77486587882331
30	21.48271676590703	22.826367420978254	28.39593971068424	27.294976102430475
31	22.57781290405131	25.244472030194142	27.23128224015767	24.946432825596872
32	21.638381454835677	23.446905404364124	26.79016159578221	28.12455154501799
33	20.338065247681467	22.178118012497006	30.871658634830574	26.612158104990957
34	18.07852371624349	20.354041732557885	34.858215765962875	26.70921878523575
35	19.4043598837534	20.869389408438835	28.993219890865056	30.73303081694271
36	18.982184805514574	21.23458640344583	32.95220698172389	26.831021809315704
37	18.815067946010792	21.904609074502023	34.781231730430044	24.49909124905714
38	20.670036809538388	22.673106274022164	30.390314008620233	26.26654290781922
39	22.01842385614377	20.00305391215337	33.84427451559793	24.134247716104934
40	21.628696594534485	19.39757341230147	34.3375661592601	24.63616383390394
41	17.610539955704134	21.46235735155124	29.901617371670298	31.025485321074324
42	20.254506817929574	19.704166398624608	32.271792526256924	27.769534257188887
43	22.108698064936636	19.324972306248007	32.57336635140208	25.992963277413278
44	20.880346732137266	19.802004695389936	30.81333739579055	28.50431117668225
45	20.97769018202589	18.21382899081635	30.066825536078223	30.74165529107954
46	22.608776180050743	18.49002424042772	32.31456143488628	26.58663814463526
47	17.51934674556882	19.823424495910093	35.75494899896011	26.902279759560972
48	17.536807771075352	19.721132577254433	33.30757773160778	29.43448192006243
49	21.36275174451197	17.142344117931906	35.97034877514794	25.52455536240818
50	19.697097157528848	17.426386225159572	34.7821507317725	28.09436588553909
51	18.425199299579592	17.523234828171493	30.331780692347333	33.719785179901585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1338.0
1	1600.0
2	1862.0
3	2343.5
4	2825.0
5	2351.0
6	1877.0
7	2003.5
8	2130.0
9	2363.5
10	2597.0
11	2752.5
12	2908.0
13	3079.0
14	3250.0
15	3323.0
16	3396.0
17	3396.5
18	3397.0
19	3352.0
20	3307.0
21	3764.0
22	4221.0
23	4557.5
24	4894.0
25	5754.0
26	8096.5
27	9579.0
28	11135.0
29	12691.0
30	14424.0
31	16157.0
32	19294.0
33	22431.0
34	25327.0
35	28223.0
36	29560.5
37	30898.0
38	34377.5
39	37857.0
40	43223.0
41	48589.0
42	56652.0
43	64715.0
44	81201.5
45	97688.0
46	122693.0
47	147698.0
48	174860.0
49	202022.0
50	196602.0
51	191182.0
52	157709.5
53	124237.0
54	105097.0
55	85957.0
56	73472.0
57	60987.0
58	55238.5
59	49490.0
60	43604.0
61	37718.0
62	32960.5
63	28203.0
64	25837.5
65	23472.0
66	20048.0
67	16624.0
68	14147.0
69	11670.0
70	9712.0
71	7754.0
72	6797.0
73	5840.0
74	4994.0
75	3195.0
76	2242.0
77	1681.0
78	1120.0
79	761.5
80	403.0
81	286.5
82	170.0
83	124.5
84	79.0
85	80.5
86	82.0
87	52.0
88	22.0
89	17.0
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1414579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.029937111021532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.86007312867295	18.180237708129603
2	13.949568012753177	7.820110282467782
3	6.511685729728798	5.475664244731036
4	3.4298636834540304	3.8455545338637256
5	2.107977351607434	2.954323629850704
6	1.3828461348436285	2.325665411633166
7	0.9612875477942033	1.8861380657195732
8	0.7065824123825482	1.5844368466269365
9	0.5474711881772253	1.3811024677233472
>10	4.771471964160792	31.7532615958975
>50	0.6658756563780824	11.758939725255772
>100	0.09065693904604474	4.947585206356771
>500	0.009854015096903073	2.055377812555449
>1k	0.003941606038761229	1.9648606940681732
>5k	8.446298654488348E-4	2.0667417751205477
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	9861	0.6970978644529574	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	8765	0.6196189820434207	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	7563	0.5346467040723777	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	3159	0.22331732621507883	No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	2574	0.18196226580487904	No Hit
TGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCTT	2169	0.15333183936704844	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	2100	0.14845406301097358	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	2017	0.14258659290149223	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	1836	0.12979126651816547	No Hit
GGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	1702	0.1203184834498462	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	1688	0.11932878969643972	No Hit
GGCCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	1634	0.11551139950472897	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	1448	0.10236261106661418	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8276964383042587E-4	0.0	0.0	0.6515719517962588	0.0
2	2.8276964383042587E-4	0.0	0.0	2.376820241216645	0.0
3	2.8276964383042587E-4	0.0	0.0	3.3540721302945964	0.0
4	2.8276964383042587E-4	0.0	0.0	4.605186419422315	0.0
5	2.8276964383042587E-4	0.0	0.0	7.620500516408062	0.0
6	2.8276964383042587E-4	0.0	0.0	9.611340193796176	0.0
7	2.8276964383042587E-4	0.0	0.0	11.298768043354242	0.0
8	2.8276964383042587E-4	0.0	0.0	14.13622003437065	0.0
9	2.8276964383042587E-4	0.0	0.0	15.08957788854493	0.0
10	2.8276964383042587E-4	0.0	0.0	17.01439085409864	0.0
11	2.8276964383042587E-4	0.0	0.0	20.07572571061779	0.0
12	2.8276964383042587E-4	0.0	0.0	22.509241265422432	0.0
13	2.8276964383042587E-4	0.0	0.0	23.48373615047304	0.0
14	2.8276964383042587E-4	0.0	0.0	23.90633538317761	0.0
15	2.8276964383042587E-4	0.0	0.0	24.624499586095933	0.0
16	2.8276964383042587E-4	0.0	0.0	26.029723331111235	0.0
17	2.8276964383042587E-4	0.0	0.0	27.591813536041464	0.0
18	2.8276964383042587E-4	0.0	0.0	29.41723297178878	0.0
19	2.8276964383042587E-4	0.0	0.0	30.486314302700663	0.0
20	2.8276964383042587E-4	0.0	0.0	31.521675353585767	0.0
21	3.534620547880323E-4	0.0	0.0	32.5916756858401	0.0
22	3.534620547880323E-4	0.0	0.0	33.69737568562802	0.0
23	3.534620547880323E-4	0.0	0.0	34.65886316706243	0.0
24	3.534620547880323E-4	0.0	0.0	35.46857404217085	0.0
25	3.534620547880323E-4	0.0	0.0	36.20200780585602	0.0
26	3.534620547880323E-4	0.0	0.0	36.84919682817291	0.0
27	3.534620547880323E-4	0.0	0.0	37.49574961879117	0.0
28	4.241544657456388E-4	0.0	0.0	38.15007857461478	0.0
29	4.241544657456388E-4	0.0	0.0	38.85028690514987	0.0
30	4.241544657456388E-4	0.0	0.0	39.62903450425886	0.0
31	5.655392876608517E-4	0.0	0.0	40.28300999802768	0.0
32	5.655392876608517E-4	0.0	0.0	40.99537742324748	0.0
33	6.362316986184582E-4	0.0	0.0	41.669500254139216	0.0
34	6.362316986184582E-4	0.0	0.0	42.371122432893465	0.0
35	6.362316986184582E-4	0.0	0.0	43.12753123013985	0.0
36	6.362316986184582E-4	0.0	0.0	43.79720043914126	0.0
37	6.362316986184582E-4	0.0	0.0	44.48864291071761	0.0
38	6.362316986184582E-4	0.0	0.0	45.19464801895122	0.0
39	6.362316986184582E-4	0.0	0.0	46.10877158504403	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACCGA	20	7.033838E-4	45.000004	42
ATATACG	20	7.033838E-4	45.000004	1
CTAGCGA	20	7.033838E-4	45.000004	16
CTTACGG	55	1.8189894E-12	45.0	2
GTCAACG	25	3.891265E-5	44.999996	1
CGTATGG	125	0.0	43.199997	2
ACGCATC	170	0.0	42.35294	29
GCGTAAG	85	0.0	42.35294	1
TACGCAT	185	0.0	41.351353	28
ATTTACG	55	6.184564E-11	40.909092	1
CGTTATG	55	6.184564E-11	40.909092	1
TTACGCC	110	0.0	40.909092	30
TACACGG	110	0.0	40.909092	2
TAACGAC	50	1.0822987E-9	40.499996	23
TTATTCG	95	0.0	40.263157	29
TAGTACG	95	0.0	40.263157	1
CGGGTAT	85	0.0	39.705883	6
GTTTACG	40	3.458972E-7	39.375004	1
ACGGATC	40	3.458972E-7	39.375004	5
TCACGCC	115	0.0	39.130436	30
>>END_MODULE