FastQCFastQC Report
Sat 14 Jan 2017
SRR2935102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267181
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC133420.5884841130902209No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC125240.5524040647835351Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT116450.5136334505273289Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG102660.4528090170127573No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC94760.41796398258454004No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC83220.3670637677362328Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG64310.2836562233010951No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC58870.25966166794799356No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC41570.18335545331404945No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT40220.17740092211429082No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC37740.16646222776214162Illumina Single End Adapter 2 (95% over 21bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA34990.15433262717004068No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC27930.12319263437722881Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCTT27390.12081082189732537Illumina Single End Adapter 2 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG24940.11000445046072634No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT24530.10819603728154038No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC22960.10127113803441365No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT22890.10096238456479656No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA22820.10065363109517944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTA302.16644E-645.00000414
ATTACGT207.03474E-445.043
ATAGTCG207.03474E-445.01
CGATCGA351.2126293E-745.041
ATCGCTC207.03474E-445.036
GATTACG800.042.18751
ACTTGAC16100.041.925476
ACGCCTA3650.041.9178125
ATCCGAT1450.041.8965510
TTGACCC14250.041.5263188
CTCAACT17400.040.991382
TAATACG4650.040.645164
TGCATTA12300.040.60975615
ACTAACG501.0822987E-940.51
GTATACG1000.040.51
CGTAAGG3950.040.443042
CTTGACC16700.040.284437
CGCCTAA3800.040.2631626
GCTTACG950.040.263161
TCAACTT17050.040.1173063
Produced by FastQC (version 0.11.5)