##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935102.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2267181
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78025001091664	33.0	31.0	34.0	30.0	34.0
2	32.10434411720988	33.0	31.0	34.0	30.0	34.0
3	32.1801038382026	34.0	31.0	34.0	30.0	34.0
4	35.765879742287886	37.0	35.0	37.0	35.0	37.0
5	30.26665758049313	37.0	35.0	37.0	0.0	37.0
6	32.70828398791274	37.0	35.0	37.0	17.0	37.0
7	34.93564519109855	37.0	35.0	37.0	32.0	37.0
8	35.48032953698888	37.0	35.0	37.0	33.0	37.0
9	37.368675019771246	39.0	37.0	39.0	34.0	39.0
10	37.266499675147244	39.0	37.0	39.0	34.0	39.0
11	37.13186640149154	39.0	37.0	39.0	34.0	39.0
12	36.85606310215197	39.0	35.0	39.0	33.0	39.0
13	36.66696571645581	39.0	35.0	39.0	33.0	39.0
14	37.29729959804709	40.0	36.0	41.0	31.0	41.0
15	37.49619505456335	40.0	36.0	41.0	32.0	41.0
16	37.77682108309835	40.0	36.0	41.0	33.0	41.0
17	37.84553990175465	40.0	36.0	41.0	33.0	41.0
18	37.845580039705695	40.0	36.0	41.0	33.0	41.0
19	37.85684557165926	40.0	36.0	41.0	33.0	41.0
20	37.69688789734918	40.0	36.0	41.0	33.0	41.0
21	37.668315851270805	39.0	36.0	41.0	33.0	41.0
22	37.75536536341827	39.0	35.0	41.0	33.0	41.0
23	37.689778187096664	39.0	35.0	41.0	33.0	41.0
24	37.67639681172346	39.0	35.0	41.0	33.0	41.0
25	37.59333418902152	39.0	35.0	41.0	33.0	41.0
26	37.569173788947595	39.0	35.0	41.0	33.0	41.0
27	37.53346909664469	39.0	35.0	41.0	33.0	41.0
28	37.451318619907276	39.0	35.0	41.0	33.0	41.0
29	37.44098596450835	39.0	35.0	41.0	33.0	41.0
30	37.31907509810642	39.0	35.0	41.0	32.0	41.0
31	32.78545118365054	38.0	32.0	40.0	9.0	41.0
32	33.618884861861495	38.0	32.0	40.0	16.0	41.0
33	35.53306639390503	38.0	33.0	40.0	28.0	41.0
34	36.31817000936405	38.0	35.0	40.0	30.0	41.0
35	36.52714891312163	39.0	35.0	40.0	31.0	41.0
36	36.713421204570786	39.0	35.0	40.0	31.0	41.0
37	36.79389823750287	39.0	35.0	40.0	31.0	41.0
38	36.805446940495706	39.0	35.0	41.0	31.0	41.0
39	36.786490359613985	39.0	35.0	40.0	31.0	41.0
40	36.75159857108894	39.0	35.0	40.0	31.0	41.0
41	36.598730317517656	39.0	35.0	40.0	31.0	41.0
42	36.591428297961215	39.0	35.0	40.0	31.0	41.0
43	36.61113559085049	39.0	35.0	40.0	31.0	41.0
44	36.610849773352896	39.0	35.0	40.0	31.0	41.0
45	36.6164968743122	39.0	35.0	40.0	31.0	41.0
46	36.57702186106888	38.0	35.0	40.0	31.0	41.0
47	36.44309078101836	38.0	35.0	40.0	31.0	41.0
48	36.45810546224585	38.0	35.0	40.0	31.0	41.0
49	36.469845592389845	38.0	35.0	40.0	31.0	41.0
50	36.27007768678372	38.0	35.0	40.0	31.0	41.0
51	35.607062250433465	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	21.0
11	29.0
12	30.0
13	21.0
14	36.0
15	74.0
16	107.0
17	271.0
18	468.0
19	974.0
20	1629.0
21	2781.0
22	4478.0
23	6717.0
24	9516.0
25	12109.0
26	15378.0
27	19180.0
28	24041.0
29	31855.0
30	43153.0
31	63403.0
32	85973.0
33	110030.0
34	198319.0
35	208214.0
36	261576.0
37	258692.0
38	403853.0
39	503935.0
40	305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01559425559759	20.412750459711862	24.079242019053616	10.49241326563693
2	31.742855996058537	25.459017167133986	23.63573971376789	19.162387123039583
3	31.23195721911925	25.945744958166113	25.69384623459706	17.12845158811758
4	28.466408284120238	23.75743268843555	27.362879276070153	20.413279751374063
5	23.244725498317074	37.52338256186869	21.57926517556384	17.652626764250407
6	25.367582032488805	32.73893879668187	25.941334194314436	15.952144976514887
7	74.90046008677737	5.561796786405672	12.725538896100488	6.812204230716472
8	73.61436956290653	6.674147322159104	12.003673284135672	7.707809830798689
9	67.53633697530105	6.713226689884928	14.45402903429413	11.296407300519897
10	42.88819463465863	22.305806197211428	19.408110777216287	15.39788839091365
11	32.56043518360466	22.353574769725046	25.157806103703233	19.92818394296706
12	28.209834150868414	19.297135958708193	30.446047316028142	22.04698257439525
13	27.424629969993575	20.67263266585244	34.119640205171095	17.783097158982898
14	20.419763574236022	24.423413922399668	33.828882652068806	21.327939851295508
15	17.73568144757741	23.984587026796714	38.367338117247805	19.912393408378072
16	24.38940693310327	25.353555803440486	28.37920748277266	21.877829780683587
17	24.039986220773727	24.35187133272553	27.122669076708032	24.485473369792707
18	25.1113166527066	22.51655249404437	30.802040066496676	21.570090786752356
19	23.06211987485781	25.447990257504806	27.75384938388245	23.736040483754937
20	20.953201354457363	30.389324892895626	29.405018831756262	19.252454920890745
21	20.37768488709106	28.006542044944798	32.94770024978156	18.66807281818258
22	19.309838958601013	22.50759864342547	33.382160489171355	24.800401908802165
23	18.541307465085495	26.357269225527208	32.48205590996044	22.619367399426864
24	21.29283017103619	26.197070282434442	27.781328442678376	24.728771103850995
25	20.99267769092984	27.994500659629733	25.1382223122018	25.874599337238624
26	18.27264783888009	24.749942770339025	27.98484108679457	28.992568303986317
27	20.969256534877452	23.33990978223618	30.3834585769729	25.307375105913465
28	16.721999699185904	24.901761262113613	28.083730412349077	30.292508626351406
29	19.21721291771588	26.409801423000633	25.842885945145095	28.530099714138395
30	22.619014538318734	23.951903266655815	28.4992684748152	24.92981372021025
31	23.67014367181094	26.11194254009715	24.66904936129934	25.548864426792566
32	23.41339310800505	27.880305983509917	23.228229241511816	25.47807166697321
33	21.81087438541519	25.2584156271599	24.570777542684063	28.35993244474085
34	18.464383743512318	25.94989107618668	27.869543719711835	27.716181460589162
35	20.558305666817073	28.331747663728656	25.10955234716593	26.00039432228834
36	20.941556937888947	27.462650754394996	24.53928468878312	27.05650761893294
37	18.059828483036863	30.08621720100865	27.467282056439252	24.38667225951523
38	21.525939040597112	27.076708917373605	23.976471221309637	27.420880820719653
39	20.353116932437242	27.461459848155044	24.200714455528693	27.98470876387902
40	23.396323451899075	23.074999305304694	28.380133743181513	25.148543499614718
41	20.694068978171572	26.110883956772753	24.188187886189944	29.006859178865735
42	21.551918439683465	23.610289606343738	29.748220367054945	25.089571586917852
43	20.403664286177417	23.593837457176996	29.332638196950313	26.669860059695278
44	20.544720514153923	22.56820253874746	27.187683735881695	29.699393211216922
45	19.876401575348417	20.585167218673764	26.22419648012223	33.31423472585559
46	22.063655261754576	22.491543463005378	27.423968355415823	28.020832919824223
47	16.717941796442364	24.11607189721509	33.26324629573025	25.902740010612295
48	18.209706238716713	26.435957252641057	28.012981760168245	27.341354748473982
49	22.714375252791903	21.9210993740685	30.266793873096148	25.097731500043448
50	19.72969956964177	22.483736410987916	28.926847922596387	28.859716096773923
51	18.83215323346482	21.822298263791026	25.029364660342512	34.31618384240164
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1531.0
1	2230.5
2	2930.0
3	3939.5
4	4949.0
5	4019.0
6	3089.0
7	3129.0
8	3169.0
9	3314.0
10	3459.0
11	3910.0
12	4361.0
13	4593.5
14	4826.0
15	4720.0
16	4614.0
17	4849.5
18	5085.0
19	5247.0
20	5409.0
21	5669.5
22	5930.0
23	6711.0
24	7492.0
25	9018.5
26	11867.5
27	13190.0
28	14804.5
29	16419.0
30	20186.5
31	23954.0
32	25895.5
33	27837.0
34	32549.0
35	37261.0
36	40608.0
37	43955.0
38	50305.5
39	56656.0
40	63607.5
41	70559.0
42	83431.5
43	96304.0
44	115776.0
45	135248.0
46	193627.5
47	252007.0
48	278820.5
49	305634.0
50	303327.0
51	301020.0
52	260116.0
53	219212.0
54	189688.0
55	160164.0
56	141955.5
57	123747.0
58	112394.5
59	101042.0
60	83612.5
61	66183.0
62	56759.0
63	47335.0
64	40197.0
65	33059.0
66	28101.5
67	23144.0
68	19969.0
69	16794.0
70	14033.5
71	11273.0
72	9160.0
73	7047.0
74	6034.0
75	3976.5
76	2932.0
77	2182.5
78	1433.0
79	1123.5
80	814.0
81	575.0
82	336.0
83	234.0
84	132.0
85	102.5
86	73.0
87	38.0
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2267181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.94993946454686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.9116386465827	17.224147926053973
2	14.15751501057263	7.630883450066916
3	6.426927399148546	5.196159130502727
4	3.504448489447186	3.7777869858889734
5	2.182860527714312	2.9413979540724755
6	1.4236857294096616	2.3020946534477766
7	1.0406055743347222	1.9630980064353547
8	0.7575045757523792	1.6331761968515095
9	0.5889607679497333	1.4285211338914507
>10	5.325533512169671	32.34016743540728
>50	0.573121465783177	9.742399372795363
>100	0.08792092478110852	4.483105372812949
>500	0.010629581354268872	1.9333580527697063
>1k	0.007206495833402625	3.6217143542579655
>5k	7.206495833402625E-4	1.4622419226302137
>10k+	7.206495833402625E-4	2.319748052115278
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	13342	0.5884841130902209	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	12524	0.5524040647835351	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	11645	0.5136334505273289	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	10266	0.4528090170127573	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	9476	0.41796398258454004	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	8322	0.3670637677362328	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	6431	0.2836562233010951	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	5887	0.25966166794799356	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	4157	0.18335545331404945	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	4022	0.17740092211429082	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3774	0.16646222776214162	Illumina Single End Adapter 2 (95% over 21bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	3499	0.15433262717004068	No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	2793	0.12319263437722881	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCTT	2739	0.12081082189732537	Illumina Single End Adapter 2 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	2494	0.11000445046072634	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	2453	0.10819603728154038	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	2296	0.10127113803441365	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	2289	0.10096238456479656	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	2282	0.10065363109517944	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6464583110038414E-4	0.0	0.0	0.5392599885055493	0.0
2	2.6464583110038414E-4	0.0	0.0	1.816793630504137	0.0
3	2.6464583110038414E-4	0.0	0.0	2.5546703152505246	0.0
4	2.6464583110038414E-4	0.0	0.0	3.620972476392489	0.0
5	2.6464583110038414E-4	0.0	0.0	6.430893695739335	0.0
6	2.6464583110038414E-4	0.0	0.0	8.07421198395717	0.0
7	2.6464583110038414E-4	0.0	0.0	9.574621523380797	0.0
8	2.6464583110038414E-4	0.0	0.0	12.109663939491377	0.0
9	2.6464583110038414E-4	0.0	0.0	12.939681481099216	0.0
10	2.6464583110038414E-4	0.0	0.0	15.190758920439082	0.0
11	2.6464583110038414E-4	0.0	0.0	18.011839372330662	0.0
12	2.6464583110038414E-4	0.0	0.0	20.904153660426758	0.0
13	3.087534696171148E-4	0.0	0.0	21.94946058563476	0.0
14	3.087534696171148E-4	0.0	0.0	22.39940260614393	0.0
15	3.087534696171148E-4	0.0	0.0	23.263294814132617	0.0
16	3.087534696171148E-4	0.0	0.0	24.50042585924988	0.0
17	3.087534696171148E-4	0.0	0.0	25.90529825364627	0.0
18	3.087534696171148E-4	0.0	0.0	27.381580914801244	0.0
19	3.087534696171148E-4	0.0	0.0	28.642750622910125	0.0
20	3.087534696171148E-4	0.0	0.0	29.653521267159526	0.0
21	3.087534696171148E-4	0.0	0.0	30.711266546429243	0.0
22	3.087534696171148E-4	0.0	0.0	31.823220113436026	0.0
23	3.087534696171148E-4	0.0	0.0	32.76205119926464	0.0
24	3.087534696171148E-4	0.0	0.0	33.51898238385025	0.0
25	3.087534696171148E-4	0.0	0.0	34.29165999538634	0.0
26	3.528611081338455E-4	0.0	0.0	34.96130216334735	0.0
27	3.528611081338455E-4	0.0	0.0	35.74857940323247	0.0
28	3.528611081338455E-4	0.0	0.0	36.45434572713868	0.0
29	3.528611081338455E-4	0.0	0.0	37.30077131027474	0.0
30	3.528611081338455E-4	0.0	0.0	38.33840350638083	0.0
31	4.410763851673069E-4	0.0	0.0	39.0530354656289	0.0
32	4.410763851673069E-4	0.0	0.0	39.8339170979291	0.0
33	4.410763851673069E-4	0.0	0.0	40.57016180005037	0.0
34	4.410763851673069E-4	0.0	0.0	41.4167638137405	0.0
35	4.410763851673069E-4	0.0	0.0	42.29521154243971	0.0
36	4.410763851673069E-4	0.0	0.0	43.029471400827724	0.0
37	4.410763851673069E-4	0.0	0.0	43.84144891828222	0.0
38	4.851840236840376E-4	0.0	0.0	44.82346138221871	0.0
39	4.851840236840376E-4	0.0	0.0	46.733807314016836	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAGTA	30	2.16644E-6	45.000004	14
ATTACGT	20	7.03474E-4	45.0	43
ATAGTCG	20	7.03474E-4	45.0	1
CGATCGA	35	1.2126293E-7	45.0	41
ATCGCTC	20	7.03474E-4	45.0	36
GATTACG	80	0.0	42.1875	1
ACTTGAC	1610	0.0	41.92547	6
ACGCCTA	365	0.0	41.91781	25
ATCCGAT	145	0.0	41.89655	10
TTGACCC	1425	0.0	41.526318	8
CTCAACT	1740	0.0	40.99138	2
TAATACG	465	0.0	40.64516	4
TGCATTA	1230	0.0	40.609756	15
ACTAACG	50	1.0822987E-9	40.5	1
GTATACG	100	0.0	40.5	1
CGTAAGG	395	0.0	40.44304	2
CTTGACC	1670	0.0	40.28443	7
CGCCTAA	380	0.0	40.26316	26
GCTTACG	95	0.0	40.26316	1
TCAACTT	1705	0.0	40.117306	3
>>END_MODULE