##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935100.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3420990
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.843752247156527	33.0	31.0	34.0	30.0	34.0
2	32.17471024469525	33.0	31.0	34.0	30.0	34.0
3	32.2258849046621	34.0	31.0	34.0	30.0	34.0
4	35.80252529238612	37.0	35.0	37.0	35.0	37.0
5	30.282642159141066	37.0	35.0	37.0	0.0	37.0
6	32.77505868184356	37.0	35.0	37.0	17.0	37.0
7	35.04351839672142	37.0	35.0	37.0	32.0	37.0
8	35.63441109152614	37.0	35.0	37.0	35.0	37.0
9	37.60089301634907	39.0	37.0	39.0	35.0	39.0
10	37.260842329267255	39.0	37.0	39.0	34.0	39.0
11	37.182212166653514	39.0	37.0	39.0	34.0	39.0
12	37.166979441623624	39.0	37.0	39.0	34.0	39.0
13	37.19037471609096	39.0	37.0	39.0	34.0	39.0
14	38.40264250991672	40.0	38.0	41.0	34.0	41.0
15	38.47428551384248	40.0	38.0	41.0	34.0	41.0
16	38.53973352742919	40.0	38.0	41.0	34.0	41.0
17	38.51030081935346	40.0	38.0	41.0	34.0	41.0
18	38.44343508750391	40.0	38.0	41.0	34.0	41.0
19	38.3820309325663	40.0	37.0	41.0	34.0	41.0
20	38.27220102952654	40.0	37.0	41.0	34.0	41.0
21	38.24337633258209	40.0	37.0	41.0	34.0	41.0
22	38.25560934115563	40.0	37.0	41.0	34.0	41.0
23	38.16385841525406	40.0	37.0	41.0	34.0	41.0
24	38.159929435631206	40.0	37.0	41.0	34.0	41.0
25	38.10937974095218	40.0	37.0	41.0	34.0	41.0
26	38.05023545815685	40.0	37.0	41.0	34.0	41.0
27	38.02427747523378	40.0	37.0	41.0	34.0	41.0
28	37.97274940879687	40.0	37.0	41.0	34.0	41.0
29	37.968727181313014	40.0	37.0	41.0	34.0	41.0
30	37.81190064864264	40.0	37.0	41.0	33.0	41.0
31	33.205967278477864	39.0	33.0	41.0	9.0	41.0
32	34.01257852259141	39.0	33.0	41.0	16.0	41.0
33	35.83282500095002	39.0	34.0	41.0	28.0	41.0
34	36.60297106977805	39.0	35.0	41.0	30.0	41.0
35	36.7266162718979	39.0	35.0	40.0	31.0	41.0
36	36.830651945781774	39.0	35.0	41.0	31.0	41.0
37	36.92560749958346	39.0	36.0	41.0	31.0	41.0
38	36.87104463912493	39.0	36.0	41.0	31.0	41.0
39	36.76216563041692	39.0	35.0	41.0	31.0	41.0
40	36.68123964115651	39.0	35.0	41.0	31.0	41.0
41	36.57035331877614	39.0	35.0	41.0	30.0	41.0
42	36.432663059523705	39.0	35.0	41.0	30.0	41.0
43	36.32773056922119	38.0	35.0	41.0	30.0	41.0
44	36.219924057071196	38.0	35.0	40.0	30.0	41.0
45	36.09523617432381	38.0	35.0	40.0	30.0	41.0
46	35.907339688218904	38.0	35.0	40.0	30.0	41.0
47	35.701074835062364	37.0	34.0	40.0	29.0	41.0
48	35.63044469583366	37.0	34.0	40.0	29.0	41.0
49	35.53350989041184	37.0	34.0	40.0	29.0	41.0
50	35.254707555415244	36.0	34.0	40.0	29.0	41.0
51	34.616999172754085	35.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	16.0
11	13.0
12	23.0
13	24.0
14	35.0
15	48.0
16	97.0
17	226.0
18	492.0
19	990.0
20	1829.0
21	3259.0
22	5831.0
23	9968.0
24	15975.0
25	22746.0
26	29092.0
27	32692.0
28	36584.0
29	43261.0
30	55467.0
31	75248.0
32	107787.0
33	139046.0
34	228146.0
35	326770.0
36	425468.0
37	486180.0
38	625835.0
39	747293.0
40	544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96712062882382	22.09109059073543	25.577479033846927	10.364309746593822
2	33.51626283619654	23.757450328706017	25.664529858315866	17.061756976781574
3	29.60163578379358	23.986477598589882	28.873542454084927	17.53834416353161
4	25.56064180251915	25.510890122449936	30.58611688429373	18.342351190737187
5	21.60161239875007	38.69026217556906	23.727809786056085	15.980315639624788
6	23.97431737596427	34.013779636888735	26.958775091420904	15.05312789572609
7	78.98576143163237	4.9502337042785856	10.980622568320866	5.083382295768184
8	80.60008944779143	4.180485765816329	9.696695985664968	5.5227288007272755
9	75.47227556935273	5.224189489007568	12.07670878897629	7.22682615266341
10	37.1407107299349	21.937333929651942	22.84394283526114	18.078012505152017
11	25.789902922838127	26.2849350626573	26.138398533757773	21.786763480746803
12	21.336484467946413	23.20340018532647	32.48901633737603	22.971099009351096
13	21.555748482164518	25.002850052177877	35.28268132908895	18.158720136568654
14	20.79208065501507	27.77915164908404	31.598221567441005	19.830546128459893
15	19.037793153443886	27.420191231193307	34.12158468747351	19.420430927889296
16	21.21526224864732	26.863393345201242	30.523269579858464	21.398074826292973
17	21.29187749744957	27.465090514734037	29.110959108328295	22.132072879488103
18	22.02324473324973	26.29233058266759	31.190941803396093	20.493482880686585
19	20.586526122555167	30.043525412234466	28.510226571840313	20.859721893370047
20	20.334435353508777	29.13346721270743	30.204911443763354	20.327185990020432
21	19.95939771820438	29.571205995925155	30.96358071786237	19.505815568008092
22	19.554719540250044	26.241526575640385	29.416952402667064	24.786801481442506
23	18.735102996501013	28.049687371199568	29.876760820698102	23.33844881160132
24	20.176966316767952	26.778125630299996	30.193686622878175	22.851221430053872
25	19.229199734579755	29.337647873861076	28.013645172888552	23.419507218670617
26	19.689592778698565	28.404789256911013	30.20853612550753	21.697081838882895
27	22.11909418033961	27.105662395973095	28.689706780785677	22.085536642901616
28	19.4110184478762	26.97242611057033	29.425312555722172	24.191242885831294
29	22.25630592313921	25.077945273151926	28.316072248091928	24.34967655561694
30	23.98051441249463	27.48496780171822	26.666666666666668	21.867851119120488
31	23.938275177653253	28.52393605359852	24.02342596733694	23.514362801411288
32	22.87638958313237	29.334607818204674	24.998523819128383	22.790478779534578
33	22.308659189299004	29.432649613123687	24.87966933548476	23.379021862092554
34	20.642796383503022	29.898713530293865	25.29039839344751	24.168091692755606
35	23.319126919400524	29.96033311994481	24.704661516110836	22.015878444543834
36	22.470892928655157	32.148120865597384	24.06893910826983	21.31204709747763
37	21.130813010268955	30.91856450910409	26.383181476707033	21.567441003919917
38	20.64495950002777	31.10897722589075	24.917085405102032	23.328977868979447
39	21.36720656885872	31.392930116720596	25.336963861338386	21.902899453082295
40	20.842913893346662	30.59924173996416	27.109404002934824	21.44844036375435
41	19.817684354528954	31.611463348328993	25.25309924904779	23.317753048094264
42	21.98287630188922	29.406458364391597	26.743135758946973	21.867529574772217
43	22.182379954340703	27.026504023689046	27.832001847418436	22.95911417455181
44	21.608130979628704	26.56236937260851	25.681074776599754	26.14842487116303
45	22.686444567215922	25.772919534988407	25.983560314411907	25.55707558338376
46	23.958035539419875	28.257609639314936	25.222289454222313	22.562065367042873
47	20.00628473044353	28.987486078591285	28.276025361079686	22.7302038298855
48	20.517277162458818	27.861408539633263	27.248048079649458	24.373266218258458
49	22.242421053554672	26.072569636274878	28.383596561229353	23.301412748941097
50	20.86963130555775	25.31311111695737	28.40771238734986	25.40954519013502
51	19.972259492135315	25.29153841431866	26.022876418814437	28.713325674731582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1009.0
1	1518.0
2	2027.0
3	10014.5
4	18002.0
5	14087.0
6	10172.0
7	9723.5
8	9275.0
9	9586.0
10	9897.0
11	10377.0
12	10857.0
13	11127.5
14	11398.0
15	11172.5
16	10947.0
17	11088.5
18	11230.0
19	11653.5
20	12077.0
21	11810.5
22	11544.0
23	13015.5
24	14487.0
25	16244.0
26	22888.0
27	27775.0
28	30732.5
29	33690.0
30	38855.0
31	44020.0
32	50754.5
33	57489.0
34	69055.5
35	80622.0
36	91481.5
37	102341.0
38	115830.5
39	129320.0
40	141350.5
41	153381.0
42	175408.0
43	197435.0
44	226791.5
45	256148.0
46	320970.5
47	385793.0
48	418984.5
49	452176.0
50	437024.5
51	421873.0
52	362898.0
53	303923.0
54	257519.5
55	211116.0
56	177656.5
57	144197.0
58	121575.5
59	98954.0
60	83981.0
61	69008.0
62	55415.5
63	41823.0
64	34124.5
65	26426.0
66	20658.5
67	14891.0
68	11481.0
69	8071.0
70	6233.0
71	4395.0
72	3525.0
73	2655.0
74	1844.0
75	885.5
76	738.0
77	519.0
78	300.0
79	256.0
80	212.0
81	153.5
82	95.0
83	67.5
84	40.0
85	64.0
86	88.0
87	62.0
88	36.0
89	18.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3420990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.81280820467658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.39058430425916	21.438837332724844
2	14.043729325976297	8.935409350513517
3	6.0087722266467605	5.734677551757024
4	3.157091296655375	4.017437596206045
5	1.8442800480954848	2.933586372288668
6	1.199355151725455	2.2892913246679605
7	0.8490956351414849	1.8908481611728871
8	0.5850952329480196	1.4890817941796728
9	0.44230126249529267	1.2663760709204148
>10	3.7200220746716104	26.15388932314067
>50	0.6577618082613437	13.964469385726868
>100	0.09279629536241632	5.025104610676246
>500	0.004953987913765414	1.0596411575451892
>1k	0.0033687117813346996	1.8885870437344137
>5k	5.944785496472999E-4	1.257114292525654
>10k+	1.9815951654909996E-4	0.6556486322198964
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	10659	0.311576473476976	No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	10142	0.2964638891081237	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8626	0.2521492316551641	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	7051	0.2061099272432834	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	6619	0.19348200374745322	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC	6211	0.18155563155694698	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	6077	0.17763863676888855	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	5299	0.15489668195463885	No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	3717	0.10865275841203863	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4615652194247865E-4	8.769391316548718E-5	0.0	0.31698426478884767	0.0
2	1.4615652194247865E-4	8.769391316548718E-5	0.0	1.0651010380036188	0.0
3	1.4615652194247865E-4	8.769391316548718E-5	0.0	1.5901244961253906	0.0
4	1.4615652194247865E-4	8.769391316548718E-5	0.0	2.3308457493298724	0.0
5	1.4615652194247865E-4	8.769391316548718E-5	0.0	4.272301292900593	0.0
6	1.4615652194247865E-4	8.769391316548718E-5	0.0	5.794989169801724	0.0
7	1.4615652194247865E-4	8.769391316548718E-5	0.0	7.007883682793577	0.0
8	1.4615652194247865E-4	8.769391316548718E-5	0.0	9.220371880654431	0.0
9	1.4615652194247865E-4	8.769391316548718E-5	0.0	9.962759318209056	0.0
10	1.4615652194247865E-4	8.769391316548718E-5	0.0	11.79962525467774	0.0
11	1.4615652194247865E-4	8.769391316548718E-5	0.0	14.359527505195864	0.0
12	1.4615652194247865E-4	8.769391316548718E-5	0.0	16.43381009590791	0.0
13	1.4615652194247865E-4	8.769391316548718E-5	0.0	17.221476823960316	0.0
14	1.7538782633097437E-4	8.769391316548718E-5	0.0	17.517443780893835	0.0
15	1.7538782633097437E-4	8.769391316548718E-5	0.0	18.077310953846695	0.0
16	1.7538782633097437E-4	8.769391316548718E-5	0.0	19.269217390287608	0.0
17	1.7538782633097437E-4	8.769391316548718E-5	0.0	20.814647222002986	0.0
18	1.7538782633097437E-4	8.769391316548718E-5	0.0	22.412839558139602	0.0
19	2.046191307194701E-4	8.769391316548718E-5	0.0	23.559291316256406	0.0
20	2.046191307194701E-4	8.769391316548718E-5	0.0	24.433190392254872	0.0
21	2.046191307194701E-4	8.769391316548718E-5	0.0	25.541173753796414	0.0
22	2.046191307194701E-4	8.769391316548718E-5	0.0	26.736880259807833	0.0
23	2.046191307194701E-4	8.769391316548718E-5	0.0	28.00084186156639	0.0
24	2.3385043510796582E-4	8.769391316548718E-5	0.0	28.944486829835807	0.0
25	2.3385043510796582E-4	8.769391316548718E-5	0.0	29.80628414581744	0.0
26	2.3385043510796582E-4	8.769391316548718E-5	0.0	30.576499785149913	0.0
27	2.3385043510796582E-4	8.769391316548718E-5	0.0	31.340196843603753	0.0
28	2.923130438849573E-4	8.769391316548718E-5	0.0	32.12549583600069	0.0
29	3.21544348273453E-4	8.769391316548718E-5	0.0	32.943387732790804	0.0
30	3.21544348273453E-4	8.769391316548718E-5	0.0	33.7651089304558	0.0
31	3.21544348273453E-4	8.769391316548718E-5	0.0	34.589051707254335	0.0
32	3.21544348273453E-4	8.769391316548718E-5	0.0	35.51188398679914	0.0
33	3.21544348273453E-4	8.769391316548718E-5	0.0	36.40440340369308	0.0
34	3.21544348273453E-4	8.769391316548718E-5	0.0	37.25079582226198	0.0
35	3.21544348273453E-4	8.769391316548718E-5	0.0	38.09800671735375	0.0
36	3.21544348273453E-4	8.769391316548718E-5	0.0	38.927620367203644	0.0
37	3.21544348273453E-4	8.769391316548718E-5	0.0	39.78035597882484	0.0
38	3.21544348273453E-4	8.769391316548718E-5	0.0	40.6408086548046	0.0
39	3.21544348273453E-4	8.769391316548718E-5	0.0	41.49827973773674	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGCG	110	0.0	42.954544	1
CGTTTTT	9055	0.0	42.912754	1
CGTTATT	1790	0.0	41.606148	1
CGCGCCG	225	0.0	40.0	27
CCGCCCG	45	1.9303116E-8	40.0	29
ACGCGCC	220	0.0	39.886364	26
TGTCGCG	335	0.0	38.955223	29
GCGCGAG	240	0.0	38.437504	1
CGACGAA	295	0.0	38.135593	29
TAATACG	270	0.0	37.500004	4
CCGTCGT	30	1.1403934E-4	37.500004	32
CGCGAGG	330	0.0	37.5	2
AAGGGCG	1815	0.0	37.438015	5
CCGCGAA	85	0.0	37.058823	30
CGTAAGG	705	0.0	37.02128	2
ACGCGAC	305	0.0	36.885246	26
TTTCGCG	55	2.752131E-9	36.81818	1
TCGCTAG	55	2.752131E-9	36.81818	1
CGCGACG	315	0.0	36.428574	27
GACGCGA	310	0.0	36.29032	25
>>END_MODULE