FastQCFastQC Report
Sat 14 Jan 2017
SRR2935099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888399
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG95100.3292481405789159No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC63800.22088361060919906No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT59670.20658503205408948No Hit
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC59480.2059272281980433No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC47170.16330846257736553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC253.892278E-545.011
CCTATCG207.035063E-445.010
CGATCGC207.035063E-445.010
TCCGGTA207.035063E-445.016
CACGTAC302.1666474E-644.99999625
GCGCGTA2750.040.09090831
AACGAGC8900.038.42696415
TAAACGG1700.038.382352
TATACGG2100.037.52
TCGCATA301.14034985E-437.49999615
ACCGTTG301.14034985E-437.49999616
CGAAAGG4950.037.2727282
ACGCATC2400.036.562529
CACAACG7850.036.11464712
CTATCGA250.002107635636.044
AGCGACT9200.035.95108419
CTAACGC2200.035.79545629
GACCGAT12150.035.740749
ACAACGA7950.035.66037413
TAAGGGA27100.035.618084
Produced by FastQC (version 0.11.5)