##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935099.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2888399
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.061897265578615	33.0	31.0	34.0	30.0	34.0
2	32.389275858356136	34.0	31.0	34.0	30.0	34.0
3	32.449359662567396	34.0	31.0	34.0	30.0	34.0
4	35.95190519038402	37.0	35.0	37.0	35.0	37.0
5	30.392502905588874	37.0	35.0	37.0	0.0	37.0
6	32.86957134384827	37.0	35.0	37.0	17.0	37.0
7	35.08325027117098	37.0	35.0	37.0	32.0	37.0
8	35.66985759238942	37.0	35.0	37.0	35.0	37.0
9	37.56639577842258	39.0	37.0	39.0	35.0	39.0
10	37.450412494949624	39.0	37.0	39.0	35.0	39.0
11	37.29559870364171	39.0	37.0	39.0	34.0	39.0
12	37.020547022762436	39.0	35.0	39.0	33.0	39.0
13	36.89929542282766	39.0	35.0	39.0	33.0	39.0
14	37.745541041940534	40.0	37.0	41.0	33.0	41.0
15	37.92296943739421	40.0	37.0	41.0	33.0	41.0
16	38.10822431388461	40.0	37.0	41.0	33.0	41.0
17	38.17892437990734	40.0	37.0	41.0	33.0	41.0
18	38.17008488093231	40.0	37.0	41.0	34.0	41.0
19	38.17712407461711	40.0	37.0	41.0	34.0	41.0
20	38.07751560639648	40.0	36.0	41.0	33.0	41.0
21	38.03079837654008	40.0	36.0	41.0	33.0	41.0
22	38.07093272086024	40.0	36.0	41.0	34.0	41.0
23	38.0478455365758	40.0	36.0	41.0	34.0	41.0
24	38.03689171752241	40.0	36.0	41.0	34.0	41.0
25	37.95074641695971	40.0	36.0	41.0	34.0	41.0
26	37.87257300670718	40.0	35.0	41.0	34.0	41.0
27	37.825397045214324	40.0	35.0	41.0	34.0	41.0
28	37.735384896615734	40.0	35.0	41.0	33.0	41.0
29	37.72286065740917	40.0	35.0	41.0	33.0	41.0
30	37.609837491288424	40.0	35.0	41.0	33.0	41.0
31	32.97580112719884	38.0	33.0	41.0	9.0	41.0
32	33.878640035535255	38.0	33.0	41.0	16.0	41.0
33	35.826823094731715	38.0	34.0	41.0	29.0	41.0
34	36.6146117624331	38.0	35.0	41.0	31.0	41.0
35	36.791278836476536	39.0	35.0	40.0	31.0	41.0
36	36.9863187184319	39.0	35.0	41.0	32.0	41.0
37	37.06809343169001	39.0	35.0	41.0	32.0	41.0
38	37.045971488011176	39.0	35.0	41.0	32.0	41.0
39	37.052892277001895	39.0	35.0	41.0	32.0	41.0
40	37.010347254655606	39.0	35.0	41.0	32.0	41.0
41	36.9096087486528	39.0	35.0	41.0	32.0	41.0
42	36.8561445285087	39.0	35.0	41.0	32.0	41.0
43	36.85797218459084	39.0	35.0	41.0	32.0	41.0
44	36.795152262550985	39.0	35.0	41.0	32.0	41.0
45	36.76048911525035	39.0	35.0	41.0	32.0	41.0
46	36.733898952326186	39.0	35.0	41.0	32.0	41.0
47	36.644683785031084	39.0	35.0	41.0	32.0	41.0
48	36.609686888826644	38.0	35.0	41.0	32.0	41.0
49	36.58257636843109	38.0	35.0	40.0	32.0	41.0
50	36.37206736326941	38.0	35.0	40.0	31.0	41.0
51	35.71545828675332	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	23.0
10	27.0
11	36.0
12	32.0
13	52.0
14	59.0
15	117.0
16	181.0
17	341.0
18	577.0
19	1047.0
20	1746.0
21	2726.0
22	4491.0
23	6895.0
24	9935.0
25	13525.0
26	17574.0
27	21594.0
28	27602.0
29	35642.0
30	48578.0
31	69862.0
32	101745.0
33	123328.0
34	233147.0
35	268729.0
36	319233.0
37	334242.0
38	486799.0
39	757740.0
40	768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48253097996503	21.614811527077805	25.5469898722441	10.355667620713065
2	32.938212483801586	23.382192003251628	25.614051244305237	18.065544268641556
3	33.01091712052248	23.849267362300015	25.71237561015635	17.427439907021157
4	29.718089502177502	26.035461167241785	24.993984556842737	19.252464773737977
5	22.560248774494106	39.02137481698339	21.413246577083015	17.00512983143949
6	28.185233411311945	32.7464799703919	23.82575952976026	15.242527088535898
7	82.12082195015302	5.895895961742128	7.267659350387532	4.7156227377173305
8	83.70775644223669	4.405693257752824	7.032269433689736	4.854280866320755
9	77.87487116565266	6.736292319724526	9.399843996622351	5.988992518000456
10	44.3355298211916	26.418303011460676	15.833719648843529	13.412447518504194
11	33.57358176623105	23.46580233548066	23.93897103551137	19.021644862776924
12	30.71667037691122	21.109998999445715	28.45742572269274	19.715904900950317
13	28.46182262215158	22.10681419014478	30.227368171779588	19.203995015924047
14	22.336803190971885	24.481728459260648	32.9050799422102	20.276388407557267
15	21.46815588843508	24.377864692516514	34.306132913077455	19.847846505970953
16	27.740765732158195	24.987475760793437	27.48429147081134	19.78746703623703
17	27.436237168064388	24.2735854707054	26.179312484182415	22.110864877047803
18	28.110797711811976	22.889012217494884	29.399331602039748	19.600858468653396
19	26.256552505384473	25.62644565380337	26.817866922125372	21.29913491868679
20	24.804467803790267	29.252191265818883	26.80602645271654	19.137314477674312
21	24.385931445066973	26.503748270235516	30.754961485584232	18.35535879911328
22	23.048685448236203	22.530093660882724	32.34480416313674	22.076416727744334
23	22.197314152234508	24.824859723327698	31.191777867254487	21.786048257183303
24	23.355429772687224	26.271093432728648	28.263581312692605	22.109895481891524
25	24.410858749085566	25.891713714067897	26.292420126166782	23.405007410679758
26	21.783555526781445	25.687690654926833	27.003506094552726	25.525247723738996
27	22.111003362070132	24.837357996592576	30.081612685781984	22.97002595555531
28	19.503018800380417	26.93460979594578	27.612459358973606	25.949912044700195
29	24.032586910603417	26.766731327631675	26.842967332421868	22.357714429343037
30	25.77656341800423	24.398256612053945	28.043217020917123	21.781962949024702
31	25.956143870704846	26.61654432091965	26.719888768830067	20.707423039545436
32	27.193680651461243	25.890986667700687	25.50412875783436	21.411203923003715
33	26.936513965002757	24.844524596497923	26.502744253823657	21.716217184675664
34	21.952334147740668	25.291623491075853	30.95735734571297	21.79868501547051
35	22.955069573144154	27.607889353236864	27.45029339783042	21.98674767578856
36	26.31547788238398	26.833827320948387	25.703962645050076	21.146732151617556
37	22.655387984831734	28.588951872646405	27.07357951584944	21.682080626672423
38	23.5196383879097	28.060804618752467	25.60380335265315	22.815753640684683
39	24.303844448083524	25.016038296648073	26.14292554456639	24.537191710702018
40	25.789650252613995	22.230931391404027	28.985330627797612	22.99408772818437
41	21.912554325077664	23.52576635014761	26.602591954920356	27.959087369854373
42	22.484670573559956	24.028259253655744	29.269259544820503	24.217810627963797
43	22.618204756337335	23.913282063869985	29.428621184261594	24.039891995531086
44	23.238479171333324	24.05349814897457	27.354600247403493	25.353422432288614
45	22.666709135406844	23.23993326406774	26.887490267099523	27.205867333425886
46	22.882087966378606	23.744122609099367	28.360832419620696	25.01295700490133
47	20.225460540597055	24.95489715929136	30.927098368334843	23.89254393177674
48	20.48376972848973	26.29847884589352	29.385483099807196	23.832268325809558
49	24.693991377230084	23.501635335007386	29.15320909611172	22.651164191650807
50	21.65434900095174	24.19987681757264	28.323441463592808	25.822332717882812
51	21.16660475232127	23.965490917286704	27.091859538796403	27.776044791595623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1119.0
1	1978.5
2	2838.0
3	4210.5
4	5583.0
5	4452.0
6	3321.0
7	3268.0
8	3215.0
9	3445.0
10	3675.0
11	4013.5
12	4352.0
13	4566.5
14	4781.0
15	4597.0
16	4413.0
17	4994.5
18	5576.0
19	5732.0
20	5888.0
21	6253.5
22	6619.0
23	8831.5
24	11044.0
25	12970.0
26	18372.5
27	21849.0
28	25881.0
29	29913.0
30	34552.5
31	39192.0
32	46203.0
33	53214.0
34	63133.0
35	73052.0
36	77421.5
37	81791.0
38	87035.0
39	92279.0
40	103116.5
41	113954.0
42	128382.0
43	142810.0
44	157846.0
45	172882.0
46	199620.0
47	226358.0
48	258662.5
49	290967.0
50	291760.0
51	292553.0
52	265471.0
53	238389.0
54	217562.5
55	196736.0
56	186363.5
57	175991.0
58	167025.0
59	158059.0
60	137940.5
61	117822.0
62	106503.0
63	95184.0
64	82774.0
65	70364.0
66	58782.0
67	47200.0
68	39548.0
69	31896.0
70	25913.5
71	19931.0
72	16663.0
73	13395.0
74	10315.0
75	5872.0
76	4509.0
77	3372.0
78	2235.0
79	1516.0
80	797.0
81	505.0
82	213.0
83	237.0
84	261.0
85	146.5
86	32.0
87	22.0
88	12.0
89	7.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2888399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.884542505458136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.975806221934	15.327106843231759
2	13.258914527338291	6.068483861542191
3	5.712799341111534	3.9220439804046032
4	3.0354687655892083	2.778612559604671
5	1.7918908648427188	2.0503301330817667
6	1.1664423453738768	1.6016099659724856
7	0.7931552123662764	1.2705695919575206
8	0.6135928910579619	1.1233434077170297
9	0.43406698361722645	0.8940081903123975
>10	4.045252120125392	20.90802537663215
>50	1.774623505826543	29.377089074281653
>100	0.3872545909074312	11.431210505099326
>500	0.00766616422117177	1.1979594929745203
>1k	0.002385028868808995	0.9656033819966454
>5k	6.814368196597128E-4	1.0840036351912437
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	9510	0.3292481405789159	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	6380	0.22088361060919906	No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT	5967	0.20658503205408948	No Hit
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	5948	0.2059272281980433	No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	4717	0.16330846257736553	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.924251116275833E-5	0.0	0.0	0.21814853141827012	0.0
2	6.924251116275833E-5	0.0	0.0	0.7412757032529093	0.0
3	6.924251116275833E-5	0.0	0.0	1.0609337560357832	0.0
4	6.924251116275833E-5	0.0	0.0	1.4683913129730346	0.0
5	6.924251116275833E-5	0.0	0.0	2.557125937240665	0.0
6	6.924251116275833E-5	0.0	0.0	3.326860312581468	0.0
7	6.924251116275833E-5	0.0	0.0	3.984006364771626	0.0
8	6.924251116275833E-5	0.0	0.0	5.144510851859455	0.0
9	6.924251116275833E-5	0.0	0.0	5.566163123585072	0.0
10	6.924251116275833E-5	0.0	0.0	6.456102498304424	0.0
11	6.924251116275833E-5	0.0	0.0	7.993078518584171	0.0
12	6.924251116275833E-5	0.0	0.0	9.169958859561993	0.0
13	6.924251116275833E-5	0.0	0.0	9.65687219805851	0.0
14	6.924251116275833E-5	0.0	0.0	9.884680059783983	0.0
15	6.924251116275833E-5	0.0	0.0	10.2711917570945	0.0
16	6.924251116275833E-5	0.0	0.0	10.99086379686463	0.0
17	6.924251116275833E-5	0.0	0.0	11.842027365332836	0.0
18	6.924251116275833E-5	0.0	0.0	12.783690895890768	0.0
19	6.924251116275833E-5	0.0	0.0	13.420548892310238	0.0
20	6.924251116275833E-5	0.0	0.0	14.00949107100508	0.0
21	6.924251116275833E-5	0.0	0.0	14.663382725170587	0.0
22	6.924251116275833E-5	0.0	0.0	15.362628224147702	0.0
23	6.924251116275833E-5	0.0	0.0	15.999313114289265	0.0
24	1.7310627790689584E-4	0.0	0.0	16.53919697382529	0.0
25	1.7310627790689584E-4	0.0	0.0	17.054707469432028	0.0
26	1.7310627790689584E-4	0.0	0.0	17.533588676633663	0.0
27	1.7310627790689584E-4	0.0	0.0	18.096184079831076	0.0
28	1.7310627790689584E-4	0.0	0.0	18.57430361940992	0.0
29	1.7310627790689584E-4	0.0	0.0	19.15988753631337	0.0
30	1.7310627790689584E-4	0.0	0.0	19.9373770729044	0.0
31	1.7310627790689584E-4	0.0	0.0	20.46043500222788	0.0
32	1.7310627790689584E-4	0.0	0.0	21.0308201879311	0.0
33	1.7310627790689584E-4	0.0	0.0	21.61404985945501	0.0
34	1.7310627790689584E-4	0.0	0.0	22.27344629325796	0.0
35	2.0772753348827498E-4	0.0	0.0	22.987544310879485	0.0
36	2.0772753348827498E-4	0.0	0.0	23.61993616532896	0.0
37	2.0772753348827498E-4	0.0	0.0	24.28192919330051	0.0
38	2.0772753348827498E-4	0.0	0.0	25.102002874256637	0.0
39	2.0772753348827498E-4	0.0	0.0	26.595079142459195	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTAC	25	3.892278E-5	45.0	11
CCTATCG	20	7.035063E-4	45.0	10
CGATCGC	20	7.035063E-4	45.0	10
TCCGGTA	20	7.035063E-4	45.0	16
CACGTAC	30	2.1666474E-6	44.999996	25
GCGCGTA	275	0.0	40.090908	31
AACGAGC	890	0.0	38.426964	15
TAAACGG	170	0.0	38.38235	2
TATACGG	210	0.0	37.5	2
TCGCATA	30	1.14034985E-4	37.499996	15
ACCGTTG	30	1.14034985E-4	37.499996	16
CGAAAGG	495	0.0	37.272728	2
ACGCATC	240	0.0	36.5625	29
CACAACG	785	0.0	36.114647	12
CTATCGA	25	0.0021076356	36.0	44
AGCGACT	920	0.0	35.951084	19
CTAACGC	220	0.0	35.795456	29
GACCGAT	1215	0.0	35.74074	9
ACAACGA	795	0.0	35.660374	13
TAAGGGA	2710	0.0	35.61808	4
>>END_MODULE