FastQCFastQC Report
Sat 14 Jan 2017
SRR2935098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207821
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC60800.27538464395437856TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT58310.2641065557397996TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC50850.2303175846230288TruSeq Adapter, Index 21 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG36280.1643249158333035No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC26460.11984667235251409No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT253.891978E-545.040
AATGCGC351.2126111E-745.033
CGTCGAA253.891978E-545.023
CATCGCG556.184564E-1140.9090921
TAGTACG1100.040.9090921
ACACGAT451.9297659E-840.016
CCGTATA403.4601544E-739.37513
TATTACG800.039.3751
TAGGACG750.039.01
ATCGGAC1100.038.86363628
ATTAGCG1050.038.571431
TATTGCG1050.038.571431
TTACGGG8600.038.45933
ACGGGAT6350.038.2677155
GCCGATA1000.038.259
TACGGGA7900.037.879754
GTCGACT2800.037.76785731
TAAGTCG301.14026254E-437.4999961
CCGTTAG301.14026254E-437.49999644
TAACGCA1400.036.96428330
Produced by FastQC (version 0.11.5)