##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935098.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2207821
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.061168002297286	33.0	31.0	34.0	30.0	34.0
2	32.38213786353151	34.0	31.0	34.0	30.0	34.0
3	32.44180121486298	34.0	31.0	34.0	30.0	34.0
4	35.94816427599883	37.0	35.0	37.0	35.0	37.0
5	30.397725177901652	37.0	35.0	37.0	0.0	37.0
6	32.87886110332314	37.0	35.0	37.0	17.0	37.0
7	35.07740573171466	37.0	35.0	37.0	32.0	37.0
8	35.64617330843397	37.0	35.0	37.0	35.0	37.0
9	37.540850458438435	39.0	37.0	39.0	35.0	39.0
10	37.4415068069377	39.0	37.0	39.0	35.0	39.0
11	37.30486755946247	39.0	37.0	39.0	34.0	39.0
12	37.05902788314813	39.0	37.0	39.0	34.0	39.0
13	36.959279307516326	39.0	37.0	39.0	33.0	39.0
14	37.97722324409452	40.0	37.0	41.0	33.0	41.0
15	38.143522957703546	40.0	37.0	41.0	33.0	41.0
16	38.28201788097858	40.0	37.0	41.0	34.0	41.0
17	38.31300318277614	40.0	37.0	41.0	34.0	41.0
18	38.291090174429904	40.0	37.0	41.0	34.0	41.0
19	38.26770240884564	40.0	37.0	41.0	34.0	41.0
20	38.186496097283246	40.0	37.0	41.0	34.0	41.0
21	38.13669994080136	40.0	37.0	41.0	34.0	41.0
22	38.158808164248825	40.0	36.0	41.0	34.0	41.0
23	38.0969748000404	40.0	36.0	41.0	34.0	41.0
24	38.083711949474164	40.0	36.0	41.0	34.0	41.0
25	38.000502758149324	40.0	36.0	41.0	34.0	41.0
26	37.91959719560599	40.0	36.0	41.0	34.0	41.0
27	37.885005623191375	40.0	36.0	41.0	34.0	41.0
28	37.81853601356269	40.0	36.0	41.0	34.0	41.0
29	37.797291537674475	40.0	36.0	41.0	33.0	41.0
30	37.68060227708678	40.0	36.0	41.0	33.0	41.0
31	33.081590853606336	38.0	33.0	41.0	9.0	41.0
32	33.95750425419452	38.0	33.0	41.0	16.0	41.0
33	35.92519185205685	38.0	34.0	41.0	29.0	41.0
34	36.775017539918316	38.0	35.0	41.0	31.0	41.0
35	36.90719311031103	39.0	35.0	41.0	32.0	41.0
36	37.06308255968215	39.0	35.0	41.0	32.0	41.0
37	37.19721888685722	39.0	35.0	41.0	32.0	41.0
38	37.15212555728023	39.0	35.0	41.0	32.0	41.0
39	37.155079601108966	39.0	35.0	41.0	33.0	41.0
40	37.075757953203635	39.0	35.0	41.0	32.0	41.0
41	36.983439327735354	39.0	35.0	41.0	32.0	41.0
42	36.9156168004562	39.0	35.0	41.0	32.0	41.0
43	36.91872937162931	39.0	35.0	41.0	32.0	41.0
44	36.85702101755532	39.0	35.0	41.0	32.0	41.0
45	36.808811946258324	39.0	35.0	41.0	32.0	41.0
46	36.736422019719896	39.0	35.0	41.0	32.0	41.0
47	36.61927574744511	38.0	35.0	40.0	32.0	41.0
48	36.61161978258201	38.0	35.0	40.0	32.0	41.0
49	36.58707476738377	38.0	35.0	40.0	32.0	41.0
50	36.37934778227039	38.0	35.0	40.0	31.0	41.0
51	35.65798721907256	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	22.0
11	13.0
12	22.0
13	29.0
14	32.0
15	74.0
16	129.0
17	218.0
18	439.0
19	775.0
20	1243.0
21	2017.0
22	3282.0
23	5131.0
24	7272.0
25	9746.0
26	12653.0
27	15845.0
28	19948.0
29	25929.0
30	34646.0
31	49877.0
32	75054.0
33	89449.0
34	167678.0
35	215780.0
36	251689.0
37	259742.0
38	381451.0
39	577033.0
40	597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31787812508351	21.791078171645257	25.31197049036131	10.579073212909924
2	32.07207468359074	23.928343828598422	25.765041640604014	18.234539847206815
3	32.28382192215764	24.06232208136439	25.314597514925353	18.339258481552626
4	29.740998024749288	25.663765314307636	25.021774863088993	19.573461797854083
5	22.90969240712902	38.88793520851554	21.077795708981842	17.124576675373593
6	27.651064103475782	32.51038014404247	23.795814968695378	16.042740783786368
7	80.403664971028	6.053978107826676	8.174621040383254	5.367735880762073
8	81.23194769865854	4.958463571095664	8.079866981970007	5.7297217482757885
9	74.9261375808999	6.821069280525912	11.026754433443653	7.226038705130533
10	43.0550302764581	23.746173263140445	17.738575726927138	15.460220733474317
11	32.457613185126874	24.15168621006866	23.823262846036886	19.567437758767582
12	28.963896982590526	21.923199389805607	28.94206550259283	20.17083812501104
13	26.710000493699443	22.794601555107956	31.266121664754525	19.229276286438076
14	21.257746891618478	25.981997634772018	31.1470902758874	21.613165197722097
15	20.02010126726759	25.44920987706884	33.39496272569198	21.135726129971587
16	25.083781701505693	24.82683152302655	28.897949607327767	21.19143716813999
17	24.748609601955955	24.77877509091543	26.630102712131098	23.842512594997512
18	25.3004659345119	23.302296698871874	29.778184010388525	21.619053356227703
19	24.456873994766788	25.943362256269868	27.436961601506642	22.162802147456702
20	24.459591606384755	27.515047641996336	27.991490252153596	20.03387049946531
21	24.35568825552434	26.077974618413357	29.92869440049714	19.63764272556516
22	23.474819743086055	23.609522692283477	29.508189296143122	23.407468268487346
23	22.19813109849032	25.22953627128286	29.913566362490435	22.65876626773638
24	23.05281995234215	24.82610682659509	29.288334516249282	22.83273870481348
25	22.938589677333443	26.553149009815556	26.77730667477119	23.730954638079808
26	20.91958541928897	27.049067836568273	27.421516508811177	24.60983023533158
27	22.437869736722316	26.159004738155854	28.16346977404418	23.239655751077645
28	19.620295304737116	26.805932183813812	28.13864892126671	25.435123590182357
29	21.04237617089429	27.07692335565247	27.659533993018453	24.221166480434782
30	24.81994690692769	25.99422688705289	26.82106022182052	22.3647659841989
31	26.222959198232104	27.372282444998934	24.664363641798857	21.740394714970098
32	24.33077681569294	27.171903881700555	25.654570728333503	22.842748574273006
33	24.861209309993882	25.430775411593604	25.427333103544175	24.280682174868343
34	22.258824424625004	26.08173398115155	28.011192936383882	23.648248657839563
35	22.057675871368197	27.841568677895534	27.254926916629564	22.845828534106705
36	24.254683690389754	27.908421923697617	24.519650823141912	23.317243562770713
37	23.068808567361213	28.25093157461588	26.53865508118638	22.14160477683653
38	21.63676312527148	27.875765290755	26.861190286712556	23.626281297260967
39	22.499785988085087	25.070827752793367	26.400645704520432	26.02874055460112
40	24.715092392000983	23.69372335891361	27.785902933254096	23.805281315831312
41	21.493680873585312	25.982903505311345	25.553792630833748	26.969622990269592
42	21.50310192719428	25.665712935967182	27.88930805531789	24.94187708152065
43	21.897789721177578	24.405556428714103	28.92095871902659	24.775695131081733
44	21.76956374633632	24.294994929389656	27.31711492915413	26.618326395119894
45	21.880442300349532	23.81157711607961	26.985747485869556	27.32223309770131
46	23.41285819819632	24.770441081953653	27.291252325256444	24.52544839459358
47	20.048409721621454	25.73261147529623	29.579390720533954	24.639588082548357
48	19.983957032748577	26.041739796840414	28.680042449093474	25.294260721317535
49	22.8059249368495	23.262438395141636	29.24403744687635	24.687599221132512
50	21.45554372387979	23.817057632842516	28.780322317796596	25.947076325481095
51	20.600809576500996	23.647433374354172	26.352770446517177	29.39898660262766
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1055.0
1	1730.0
2	2405.0
3	3200.0
4	3995.0
5	3266.0
6	2537.0
7	2616.5
8	2696.0
9	2948.5
10	3201.0
11	3386.5
12	3572.0
13	3798.0
14	4024.0
15	4206.0
16	4388.0
17	4338.0
18	4288.0
19	4536.5
20	4785.0
21	5483.5
22	6182.0
23	6798.0
24	7414.0
25	9659.5
26	14681.0
27	17457.0
28	19579.0
29	21701.0
30	25938.5
31	30176.0
32	33726.5
33	37277.0
34	43511.5
35	49746.0
36	55221.0
37	60696.0
38	65829.0
39	70962.0
40	80014.5
41	89067.0
42	100196.5
43	111326.0
44	127609.5
45	143893.0
46	164934.5
47	185976.0
48	209818.5
49	233661.0
50	234421.0
51	235181.0
52	208231.5
53	181282.0
54	161073.5
55	140865.0
56	129677.0
57	118489.0
58	111542.0
59	104595.0
60	96105.5
61	87616.0
62	77523.5
63	67431.0
64	60748.0
65	54065.0
66	46134.5
67	38204.0
68	31700.0
69	25196.0
70	20515.5
71	15835.0
72	13421.0
73	11007.0
74	8623.5
75	5249.0
76	4258.0
77	2898.5
78	1539.0
79	1302.5
80	1066.0
81	688.5
82	311.0
83	226.0
84	141.0
85	111.5
86	82.0
87	57.5
88	33.0
89	16.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2207821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.838767650922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.78576160832283	15.68251485719753
2	13.606431510812412	6.487211186888814
3	6.180087443230492	4.419770058646569
4	3.1860035062255867	3.038015892797384
5	2.005101691098625	2.389957666528545
6	1.323503796104832	1.8930419688273799
7	0.8763516743344231	1.4623800761468346
8	0.6480426044541726	1.23588296603851
9	0.47840399254755595	1.0264105459453146
>10	3.9046607154555235	20.691323335269942
>50	1.6710935615026314	28.55938792894164
>100	0.32575940494316963	10.307610668780304
>500	0.005150336176236504	0.7808659648512389
>1k	0.003004362769471294	1.1561563682938942
>5k	6.43792022029563E-4	0.8694705148461516
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	6080	0.27538464395437856	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	5831	0.2641065557397996	TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	5085	0.2303175846230288	TruSeq Adapter, Index 21 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3628	0.1643249158333035	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2646	0.11984667235251409	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.529352696618068E-5	0.0	0.0	0.2800951707588613	0.0
2	1.3588058089854206E-4	0.0	0.0	1.002708099977308	0.0
3	1.3588058089854206E-4	0.0	0.0	1.4430517691425164	0.0
4	1.3588058089854206E-4	0.0	0.0	1.9994374543950801	0.0
5	1.3588058089854206E-4	0.0	0.0	3.376179500059108	0.0
6	1.3588058089854206E-4	0.0	0.0	4.328747665684854	0.0
7	1.3588058089854206E-4	0.0	0.0	5.152908682361478	0.0
8	1.3588058089854206E-4	0.0	0.0	6.615617842207317	0.0
9	1.3588058089854206E-4	0.0	0.0	7.1211841902038255	0.0
10	1.3588058089854206E-4	0.0	0.0	8.20220479830566	0.0
11	1.3588058089854206E-4	0.0	0.0	9.996779630232705	0.0
12	1.3588058089854206E-4	0.0	0.0	11.443726642694312	0.0
13	1.3588058089854206E-4	0.0	0.0	12.02307614611873	0.0
14	1.3588058089854206E-4	0.0	0.0	12.284283916132694	0.0
15	1.3588058089854206E-4	0.0	0.0	12.754566606622548	0.0
16	1.3588058089854206E-4	0.0	0.0	13.660844787688857	0.0
17	1.3588058089854206E-4	0.0	0.0	14.736701933716546	0.0
18	1.3588058089854206E-4	0.0	0.0	15.926562887118113	0.0
19	1.3588058089854206E-4	0.0	0.0	16.70552096388249	0.0
20	1.3588058089854206E-4	0.0	0.0	17.405849477833574	0.0
21	1.3588058089854206E-4	0.0	0.0	18.203377900654083	0.0
22	1.3588058089854206E-4	0.0	0.0	19.00996502886783	0.0
23	2.2646763483090342E-4	0.0	0.0	19.75599471152779	0.0
24	2.2646763483090342E-4	0.0	0.0	20.379143055528505	0.0
25	2.2646763483090342E-4	0.0	0.0	20.956952579036074	0.0
26	2.2646763483090342E-4	0.0	0.0	21.4765599203921	0.0
27	2.2646763483090342E-4	0.0	0.0	22.017228751787396	0.0
28	2.2646763483090342E-4	0.0	0.0	22.560252846584937	0.0
29	2.2646763483090342E-4	0.0	0.0	23.180275937224984	0.0
30	2.2646763483090342E-4	0.0	0.0	23.922410376565853	0.0
31	2.2646763483090342E-4	0.0	0.0	24.50153341235544	0.0
32	2.2646763483090342E-4	0.0	0.0	25.137590411541517	0.0
33	2.2646763483090342E-4	0.0	0.0	25.755348825833252	0.0
34	2.2646763483090342E-4	0.0	0.0	26.445441002689982	0.0
35	2.2646763483090342E-4	0.0	0.0	27.17444031921066	0.0
36	2.2646763483090342E-4	0.0	0.0	27.806330313915847	0.0
37	2.717611617970841E-4	0.0	0.0	28.482517377993958	0.0
38	2.717611617970841E-4	0.0	0.0	29.270624747205503	0.0
39	2.717611617970841E-4	0.0	0.0	30.396984175800483	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	25	3.891978E-5	45.0	40
AATGCGC	35	1.2126111E-7	45.0	33
CGTCGAA	25	3.891978E-5	45.0	23
CATCGCG	55	6.184564E-11	40.909092	1
TAGTACG	110	0.0	40.909092	1
ACACGAT	45	1.9297659E-8	40.0	16
CCGTATA	40	3.4601544E-7	39.375	13
TATTACG	80	0.0	39.375	1
TAGGACG	75	0.0	39.0	1
ATCGGAC	110	0.0	38.863636	28
ATTAGCG	105	0.0	38.57143	1
TATTGCG	105	0.0	38.57143	1
TTACGGG	860	0.0	38.4593	3
ACGGGAT	635	0.0	38.267715	5
GCCGATA	100	0.0	38.25	9
TACGGGA	790	0.0	37.87975	4
GTCGACT	280	0.0	37.767857	31
TAAGTCG	30	1.14026254E-4	37.499996	1
CCGTTAG	30	1.14026254E-4	37.499996	44
TAACGCA	140	0.0	36.964283	30
>>END_MODULE