FastQCFastQC Report
Sat 14 Jan 2017
SRR2935095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148692
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC156920.4983656705705099No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC146260.4645103427073845TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG130490.4144260537391399No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT120920.38403248078884816TruSeq Adapter, Index 23 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC107040.3399506842841408No Hit
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC98270.3120978488845527TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC55690.1768670927483539No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT48770.15488971293476783No Hit
TCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC41910.13310288843748452TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.11160189691465534No Hit
ACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC33520.10645690337448058TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT3100.039.91935727
TAACGCC4300.039.7674430
TACGGGT4350.039.3103454
ACCGGTA2500.038.69999741
CGTAAGG4900.038.571432
CGTTTTT32750.038.2671781
TAATACG6900.037.8260884
CGTTATT5600.037.7678571
CTTACGG2950.037.3728832
ATACGGG7950.037.0754743
TATACGC3100.037.01613226
TCTAACG4700.036.86170228
TTACGCC2950.036.6101730
TACGCAT3150.036.42857428
TACGGGC7250.036.3103454
TGTTACG751.8189894E-1236.0000041
GACGCGA3500.036.026
TAACGGG11150.035.9192853
CGGTATT2650.035.66037443
GACCGAT13650.035.6043979
Produced by FastQC (version 0.11.5)