##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935095.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3148692
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864490715509806	33.0	31.0	34.0	30.0	34.0
2	32.18868660383423	34.0	31.0	34.0	30.0	34.0
3	32.25241878214827	34.0	31.0	34.0	30.0	34.0
4	35.81462905866944	37.0	35.0	37.0	35.0	37.0
5	30.33451795221635	37.0	35.0	37.0	0.0	37.0
6	32.78162456029361	37.0	35.0	37.0	17.0	37.0
7	34.98998187183758	37.0	35.0	37.0	32.0	37.0
8	35.521283123277854	37.0	35.0	37.0	33.0	37.0
9	37.42913851211868	39.0	37.0	39.0	35.0	39.0
10	37.327417543538715	39.0	37.0	39.0	34.0	39.0
11	37.18739495638189	39.0	37.0	39.0	34.0	39.0
12	37.04266184180606	39.0	37.0	39.0	33.0	39.0
13	36.95982109396537	39.0	37.0	39.0	33.0	39.0
14	37.96591537057292	40.0	37.0	41.0	33.0	41.0
15	38.07426575860707	40.0	37.0	41.0	33.0	41.0
16	38.22679766709478	40.0	37.0	41.0	33.0	41.0
17	38.23190740790144	40.0	37.0	41.0	34.0	41.0
18	38.18986868197969	40.0	37.0	41.0	34.0	41.0
19	38.184098984594236	40.0	37.0	41.0	34.0	41.0
20	38.06665307372077	40.0	37.0	41.0	33.0	41.0
21	38.02588439898218	40.0	37.0	41.0	33.0	41.0
22	38.05201461432239	40.0	37.0	41.0	34.0	41.0
23	37.98507316688961	40.0	36.0	41.0	34.0	41.0
24	37.940385404479066	40.0	36.0	41.0	33.0	41.0
25	37.87587671325109	40.0	36.0	41.0	33.0	41.0
26	37.828056539032715	40.0	36.0	41.0	33.0	41.0
27	37.75689556171261	40.0	36.0	41.0	33.0	41.0
28	37.72563813799508	40.0	36.0	41.0	33.0	41.0
29	37.73001042972765	40.0	36.0	41.0	33.0	41.0
30	37.543487581510036	39.0	36.0	41.0	33.0	41.0
31	33.001146507819755	38.0	33.0	40.0	9.0	41.0
32	33.8826125260902	38.0	33.0	40.0	16.0	41.0
33	35.77604351267129	38.0	34.0	40.0	28.0	41.0
34	36.55783989034177	38.0	35.0	40.0	30.0	41.0
35	36.7360891443177	39.0	35.0	40.0	31.0	41.0
36	36.89158514075051	39.0	35.0	40.0	31.0	41.0
37	36.959289762225076	39.0	35.0	41.0	32.0	41.0
38	36.99210116454706	39.0	35.0	41.0	32.0	41.0
39	36.99152378193866	39.0	35.0	41.0	32.0	41.0
40	36.9359718257613	39.0	35.0	41.0	32.0	41.0
41	36.815229943100185	39.0	35.0	41.0	31.0	41.0
42	36.800438404264376	39.0	35.0	41.0	31.0	41.0
43	36.8179145499147	39.0	35.0	41.0	31.0	41.0
44	36.775473434683356	39.0	35.0	41.0	31.0	41.0
45	36.770066745175455	39.0	35.0	40.0	32.0	41.0
46	36.71494004494565	39.0	35.0	40.0	31.0	41.0
47	36.59226497860064	39.0	35.0	40.0	31.0	41.0
48	36.60172636764727	39.0	35.0	40.0	31.0	41.0
49	36.5940450828471	39.0	35.0	40.0	31.0	41.0
50	36.37970592233219	38.0	35.0	40.0	31.0	41.0
51	35.739756698972144	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	19.0
11	22.0
12	27.0
13	36.0
14	53.0
15	80.0
16	166.0
17	315.0
18	567.0
19	993.0
20	1833.0
21	3112.0
22	5014.0
23	7852.0
24	11335.0
25	15194.0
26	19275.0
27	24071.0
28	30702.0
29	39834.0
30	53831.0
31	76311.0
32	107150.0
33	134181.0
34	236782.0
35	294525.0
36	378747.0
37	381421.0
38	577674.0
39	746970.0
40	589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24439862647728	20.890865159247078	25.339982443503523	11.524753770772117
2	30.957299094354102	26.194781833218368	24.75529521464786	18.092623857779675
3	30.649202907111906	25.894149062531362	26.3211200079271	17.13552802242963
4	27.67069627642208	24.61676149969575	28.286761614028936	19.425780609853234
5	23.438748534312026	37.46670045847609	21.162406484978526	17.932144522233358
6	25.79839501608922	33.160086791594736	24.826372347628794	16.215145844687253
7	76.65614801320675	5.629543950313336	11.74236794198988	5.971940094490029
8	76.02010612660749	6.486090097094285	10.358459957341017	7.135343818957206
9	69.69033490732025	6.422952768959302	13.310225325309686	10.576486998410768
10	42.38372632191399	19.587244481200447	20.00052720304177	18.028501993843793
11	28.698932763191827	22.450211071772024	25.962050273573915	22.888805891462233
12	25.27760733663375	19.159511314539497	31.908710029434445	23.654171319392304
13	24.32422732995161	20.577338145490252	35.00710771329809	20.09132681126004
14	19.740133363314037	24.280304329543824	33.442680325671745	22.5368819814704
15	18.04962187473402	23.46891979272663	36.75484931520771	21.72660901733164
16	21.6467345805814	24.53510854666001	30.00274399655476	23.815412876203833
17	22.319013736497567	24.555910835356396	28.352884308785995	24.77219111936004
18	22.41384676557758	22.87324387396417	31.38804938685651	23.324859973601736
19	21.873940036053067	25.11433318978166	28.74752436884903	24.26420240531624
20	22.1676492969144	27.11630734285856	29.61979132922496	21.09625203100208
21	21.774660716259323	26.523934382912017	31.142550621019776	20.558854279808887
22	20.397326890022903	23.18041904384424	30.62728904573709	25.79496502039577
23	20.727209901762382	24.459743919062266	30.282161608693386	24.530884570481966
24	22.34365889073939	23.662778067845313	28.850360721213764	25.14320232020153
25	21.163581576095723	26.721190894504765	25.957762778957104	26.157464750442404
26	19.59245299317939	24.824403276027	28.06149347093968	27.52165025985393
27	21.53954721516109	25.28430853192373	28.37886335024194	24.79728090267324
28	18.49043983978109	24.488803604798438	29.152740248966875	27.8680163064536
29	21.892741493928273	23.22237297265023	27.09382181553483	27.791063717886665
30	23.54657108412001	25.984821633872095	27.011089049040045	23.457518232967846
31	24.080792913374825	25.62025755456552	26.743644662609107	23.555304869450552
32	25.556453282823473	24.474384919198194	26.28262783403394	23.68653396394439
33	26.628231659368396	23.300659448431286	26.3121003896221	23.75900850257821
34	22.662966082424067	21.382751949063294	31.432321738677523	24.52196022983512
35	22.764659102890977	23.90290952560619	28.465534259940316	24.866897111562515
36	25.347636415375018	24.444435975319276	27.938966402556996	22.26896120674871
37	22.773234092124603	24.760440208188033	31.610109848788003	20.856215850899357
38	25.04328781602011	23.16511110010125	29.041710018001126	22.749891065877513
39	27.624105501586055	21.062174388603268	28.48109627743838	22.8326238323723
40	24.89779883202295	20.557456874156	31.890543755946915	22.65420053787414
41	21.437060214209584	23.014445363344525	29.996995577846292	25.551498844599596
42	22.75633818741242	21.687735732805876	31.820133566573038	23.735792513208658
43	24.576236735762023	21.175872393997253	30.154426028331766	24.093464841908958
44	23.412864770514233	21.014726114843878	28.84013425257218	26.732274862069712
45	22.894744865487002	19.52464705979499	29.02741836927842	28.553189705439593
46	25.061898718579016	21.174443229124982	29.638656305538934	24.125001746757064
47	20.459098571724386	21.806991601592028	34.118389477281355	23.615520349402228
48	21.554664603587774	21.85590079944307	31.42685915294351	25.16257544402565
49	24.05265424500078	19.4315290285617	33.14484236629051	23.370974360147006
50	22.651913874078506	19.382365756955586	31.824166987434783	26.141553381531125
51	21.325363039636777	19.219472720736103	29.203110370909574	30.25205386871755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1792.0
1	2549.5
2	3307.0
3	5778.0
4	8249.0
5	6462.5
6	4676.0
7	4543.0
8	4410.0
9	4605.5
10	4801.0
11	5177.5
12	5554.0
13	5549.5
14	5545.0
15	5779.5
16	6014.0
17	6146.0
18	6278.0
19	6411.0
20	6544.0
21	7409.0
22	8274.0
23	9420.5
24	10567.0
25	12099.5
26	17697.0
27	21762.0
28	24034.0
29	26306.0
30	30753.5
31	35201.0
32	41063.0
33	46925.0
34	52495.5
35	58066.0
36	66115.0
37	74164.0
38	82074.5
39	89985.0
40	99598.5
41	109212.0
42	126304.0
43	143396.0
44	169638.0
45	195880.0
46	266300.5
47	336721.0
48	360962.0
49	385203.0
50	378337.0
51	371471.0
52	316995.0
53	262519.0
54	228928.5
55	195338.0
56	173974.0
57	152610.0
58	143927.0
59	135244.0
60	122675.0
61	110106.0
62	100242.0
63	90378.0
64	80576.0
65	70774.0
66	61638.5
67	52503.0
68	45055.5
69	37608.0
70	30716.0
71	23824.0
72	19094.5
73	14365.0
74	11631.0
75	6936.0
76	4975.0
77	4131.5
78	3288.0
79	2270.0
80	1252.0
81	882.0
82	512.0
83	397.0
84	282.0
85	241.5
86	201.0
87	133.5
88	66.0
89	37.5
90	9.0
91	7.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3148692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.999374477245563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.26120060253399	16.70751018822679
2	14.165307233810138	7.365782547141304
3	6.395596862798941	4.988445535242198
4	3.4558598738777384	3.594007800073358
5	2.1128131623859576	2.746591030466298
6	1.3478904059195917	2.1026584450673997
7	0.9307344542540095	1.6938959530517341
8	0.6709787750874119	1.395602275182492
9	0.50902263951514	1.1910843199935093
>10	5.143906315166449	32.20252522060322
>50	0.9093875802139619	15.079229121192334
>100	0.08308352266641168	3.8566892411040445
>500	0.007109285885125897	1.2307004525874463
>1k	0.006170323598411081	3.000915654856856
>5k	2.6827493906135134E-4	0.5369340068586853
>10k+	6.706873476533784E-4	2.3074282083522473
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	15692	0.4983656705705099	No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	14626	0.4645103427073845	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	13049	0.4144260537391399	No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	12092	0.38403248078884816	TruSeq Adapter, Index 23 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	10704	0.3399506842841408	No Hit
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	9827	0.3120978488845527	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC	5569	0.1768670927483539	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT	4877	0.15488971293476783	No Hit
TCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	4191	0.13310288843748452	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3514	0.11160189691465534	No Hit
ACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	3352	0.10645690337448058	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.270368775351797E-4	0.0	0.0	0.4044854180720121	0.0
2	1.270368775351797E-4	0.0	0.0	1.4804242523562166	0.0
3	1.270368775351797E-4	0.0	0.0	2.068636754563482	0.0
4	1.270368775351797E-4	0.0	0.0	2.9440478776584054	0.0
5	1.270368775351797E-4	0.0	0.0	5.321161930096688	0.0
6	1.270368775351797E-4	0.0	0.0	6.885525799284275	0.0
7	1.270368775351797E-4	0.0	0.0	8.276674885952643	0.0
8	1.270368775351797E-4	0.0	0.0	10.458723812935657	0.0
9	1.270368775351797E-4	0.0	0.0	11.240127646654548	0.0
10	1.270368775351797E-4	0.0	0.0	13.224570710631589	0.0
11	1.270368775351797E-4	0.0	0.0	15.742695697133922	0.0
12	1.270368775351797E-4	0.0	0.0	18.21969249453424	0.0
13	1.270368775351797E-4	0.0	0.0	19.03942970604937	0.0
14	1.270368775351797E-4	0.0	0.0	19.41539534511473	0.0
15	1.270368775351797E-4	0.0	0.0	20.132804351775277	0.0
16	1.270368775351797E-4	0.0	0.0	21.305862878935127	0.0
17	1.270368775351797E-4	0.0	0.0	22.62752279359175	0.0
18	1.270368775351797E-4	0.0	0.0	23.994757188064124	0.0
19	1.270368775351797E-4	0.0	0.0	25.169340157754394	0.0
20	1.270368775351797E-4	0.0	0.0	26.057042098750845	0.0
21	1.270368775351797E-4	0.0	0.0	27.056822324952712	0.0
22	1.270368775351797E-4	0.0	0.0	28.080898353983176	0.0
23	1.270368775351797E-4	0.0	0.0	28.98829101099758	0.0
24	1.270368775351797E-4	0.0	0.0	29.769536048619553	0.0
25	1.270368775351797E-4	0.0	0.0	30.48002154545443	0.0
26	1.270368775351797E-4	0.0	0.0	31.12117666637448	0.0
27	1.270368775351797E-4	0.0	0.0	31.801332108697835	0.0
28	1.270368775351797E-4	0.0	0.0	32.42670289758414	0.0
29	1.270368775351797E-4	0.0	0.0	33.139760891189106	0.0
30	1.270368775351797E-4	0.0	0.0	33.89563031252342	0.0
31	1.270368775351797E-4	0.0	0.0	34.545487459554636	0.0
32	1.270368775351797E-4	0.0	0.0	35.245810006186694	0.0
33	1.587960969189746E-4	0.0	0.0	35.93511210369258	0.0
34	1.587960969189746E-4	0.0	0.0	36.64855120793015	0.0
35	1.587960969189746E-4	0.0	0.0	37.391653423072185	0.0
36	1.587960969189746E-4	0.0	0.0	38.05967684359093	0.0
37	1.587960969189746E-4	0.0	0.0	38.75949124271285	0.0
38	1.9055531630276953E-4	0.0	0.0	39.52590472488259	0.0
39	1.9055531630276953E-4	0.0	0.0	40.58034256764396	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	310	0.0	39.919357	27
TAACGCC	430	0.0	39.76744	30
TACGGGT	435	0.0	39.310345	4
ACCGGTA	250	0.0	38.699997	41
CGTAAGG	490	0.0	38.57143	2
CGTTTTT	3275	0.0	38.267178	1
TAATACG	690	0.0	37.826088	4
CGTTATT	560	0.0	37.767857	1
CTTACGG	295	0.0	37.372883	2
ATACGGG	795	0.0	37.075474	3
TATACGC	310	0.0	37.016132	26
TCTAACG	470	0.0	36.861702	28
TTACGCC	295	0.0	36.61017	30
TACGCAT	315	0.0	36.428574	28
TACGGGC	725	0.0	36.310345	4
TGTTACG	75	1.8189894E-12	36.000004	1
GACGCGA	350	0.0	36.0	26
TAACGGG	1115	0.0	35.919285	3
CGGTATT	265	0.0	35.660374	43
GACCGAT	1365	0.0	35.604397	9
>>END_MODULE