SRR2935093.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1723677
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	7736	0.44880798432652985	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	6435	0.37332980599033344	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	5683	0.3297021425707949	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2493	0.14463266609695435	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2027	0.11759743849920838	TruSeq Adapter, Index 27 (95% over 23bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1897	0.11005542221657538	No Hit
TGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCTT	1784	0.10349966960167131	Illumina Single End Adapter 1 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1728	0.10025080104915247	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	25	3.89162E-5	45.0	14
TAGACCG	20	7.034269E-4	45.0	1
CGACCTA	50	2.1827873E-11	45.0	33
TAACGGT	25	3.89162E-5	45.0	3
CCCGTTG	20	7.034269E-4	45.0	29
CGTAGCA	20	7.034269E-4	45.0	39
AACGCGT	20	7.034269E-4	45.0	34
CTCGCGA	20	7.034269E-4	45.0	37
CGTACGT	25	3.89162E-5	45.0	18
ATACGTA	40	6.8193913E-9	45.0	30
AATCGTC	20	7.034269E-4	45.0	18
TATAGCG	65	0.0	41.53846	1
CGTATGG	175	0.0	41.142857	2
CGTAAGG	360	0.0	40.625004	2
ACGTTAG	50	1.0822987E-9	40.5	1
TTACGCC	100	0.0	40.5	30
ATTGCGG	300	0.0	40.5	2
CTACGGG	490	0.0	40.408165	3
GCTACGA	130	0.0	39.80769	10
TCGTGCG	85	0.0	39.705883	45