##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1723677
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95947790682361	33.0	31.0	34.0	30.0	34.0
2	32.2728527444527	34.0	31.0	34.0	30.0	34.0
3	32.3365624766125	34.0	31.0	34.0	30.0	34.0
4	35.866311959839344	37.0	35.0	37.0	35.0	37.0
5	30.38311992328029	37.0	35.0	37.0	0.0	37.0
6	32.84343180305823	37.0	35.0	37.0	17.0	37.0
7	35.04201135131466	37.0	35.0	37.0	32.0	37.0
8	35.58117153039694	37.0	35.0	37.0	35.0	37.0
9	37.385871598913255	39.0	37.0	39.0	35.0	39.0
10	37.33900550973297	39.0	37.0	39.0	34.0	39.0
11	37.28890331541234	39.0	37.0	39.0	34.0	39.0
12	37.1663293064768	39.0	37.0	39.0	34.0	39.0
13	37.116946504478506	39.0	37.0	39.0	33.0	39.0
14	38.316454881047896	40.0	38.0	41.0	34.0	41.0
15	38.41787295415556	40.0	38.0	41.0	34.0	41.0
16	38.47715552275746	40.0	38.0	41.0	34.0	41.0
17	38.47990835870061	40.0	38.0	41.0	34.0	41.0
18	38.42609027097304	40.0	38.0	41.0	34.0	41.0
19	38.41844034584206	40.0	37.0	41.0	34.0	41.0
20	38.33701673805475	40.0	37.0	41.0	34.0	41.0
21	38.2722012302769	40.0	37.0	41.0	34.0	41.0
22	38.263147329807154	40.0	37.0	41.0	34.0	41.0
23	38.19368942092979	40.0	37.0	41.0	34.0	41.0
24	38.184180098707586	40.0	37.0	41.0	34.0	41.0
25	38.1179925241214	40.0	37.0	41.0	34.0	41.0
26	38.04605619266255	40.0	37.0	41.0	34.0	41.0
27	38.02081538478497	40.0	37.0	41.0	34.0	41.0
28	37.967891896219534	40.0	37.0	41.0	34.0	41.0
29	37.98552919137402	40.0	37.0	41.0	34.0	41.0
30	37.88179629942269	40.0	36.0	41.0	33.0	41.0
31	33.34404589723017	39.0	34.0	41.0	9.0	41.0
32	34.279410817688	39.0	34.0	41.0	16.0	41.0
33	36.24674344439242	39.0	34.0	41.0	30.0	41.0
34	37.11354969637583	39.0	35.0	41.0	32.0	41.0
35	37.2722012302769	39.0	36.0	41.0	33.0	41.0
36	37.41745640279472	39.0	36.0	41.0	33.0	41.0
37	37.538079350133465	40.0	36.0	41.0	33.0	41.0
38	37.513832347939896	40.0	36.0	41.0	33.0	41.0
39	37.52114462280346	40.0	36.0	41.0	33.0	41.0
40	37.45964412125938	40.0	36.0	41.0	33.0	41.0
41	37.38484878547431	40.0	35.0	41.0	33.0	41.0
42	37.31687027209854	40.0	35.0	41.0	33.0	41.0
43	37.309782517258164	39.0	35.0	41.0	33.0	41.0
44	37.25729994656771	39.0	35.0	41.0	33.0	41.0
45	37.22181243933753	39.0	35.0	41.0	33.0	41.0
46	37.16179887531133	39.0	35.0	41.0	32.0	41.0
47	37.06853662258068	39.0	35.0	41.0	32.0	41.0
48	37.08150366919092	39.0	35.0	41.0	32.0	41.0
49	37.05864845907905	39.0	35.0	41.0	32.0	41.0
50	36.87407211443907	39.0	35.0	41.0	32.0	41.0
51	36.28114142034732	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	15.0
11	10.0
12	20.0
13	32.0
14	24.0
15	53.0
16	87.0
17	172.0
18	292.0
19	558.0
20	910.0
21	1491.0
22	2504.0
23	3915.0
24	5562.0
25	7341.0
26	9076.0
27	11288.0
28	13979.0
29	18062.0
30	24407.0
31	34083.0
32	51871.0
33	61803.0
34	109722.0
35	160017.0
36	201537.0
37	201115.0
38	301858.0
39	501397.0
40	463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03712354460841	21.61953776722669	24.21242494968605	11.130913738478846
2	31.50683103620922	24.54914696895068	25.23535441965055	18.70866757518955
3	31.704141785264873	23.807418675308657	26.563851580081423	17.92458795934505
4	28.12539704364565	26.2773709923611	25.928059607455456	19.669172356537796
5	22.63185039888564	39.1645882610257	20.50291324882794	17.700648091260717
6	26.488953556843885	33.437761251092866	23.93899785168567	16.134287340377576
7	78.53722014043234	6.300542387001741	9.263800584448246	5.898436888117669
8	77.69338454942545	6.122202709672404	9.192963646901363	6.9914490940007905
9	70.44086566102582	8.846262959939711	12.14247216850953	8.570399210524942
10	37.18399676969641	27.95593373932587	19.074049256328188	15.78602023464953
11	29.934320641280237	24.92932260510525	25.196135934980855	19.94022081863365
12	27.06934071754743	21.815340113025815	29.829428599441776	21.285890569984982
13	24.182082838025917	23.992256089743034	31.67739663521646	20.14826443701459
14	19.017832227267636	28.407700514655588	29.347551774491393	23.22691548358538
15	18.16169734816906	26.170332376657573	34.8193426030515	20.848627672121864
16	19.23289572234241	26.695024647889365	31.91038692283995	22.16169270692827
17	19.407580422550165	25.9713391778158	27.97275823718713	26.64832216244691
18	20.581640295716657	26.714923967773547	29.94772222405938	22.755713512450416
19	22.99357710290269	26.993456430642166	27.663767631638642	22.3491988348165
20	24.678753617992232	26.90602705727349	27.59658567121334	20.818633653520934
21	22.592631914215946	27.966840655180757	28.156667403463643	21.283860027139657
22	20.731378326681853	26.24058915910579	27.050775754390177	25.977256759822172
23	21.224858253605518	26.826255731207183	28.192637019580815	23.756248995606484
24	22.359409564552987	24.328862077987928	28.689655892606332	24.622072464852753
25	21.09374320130744	27.3374303886401	25.95318032322761	25.61564608682485
26	19.374975706005245	27.579413080292888	27.944446668372326	25.101164545329546
27	21.997160720947136	26.498410084952113	27.8605562411055	23.643872952995252
28	18.479912419786306	26.722465984056175	29.92010684136297	24.877514754794547
29	19.800055346796412	23.937025324350213	28.818508340019623	27.444410988833756
30	20.975565607709566	24.56092411745356	30.0252309452409	24.438279329595975
31	22.401412793696267	26.83582829033514	26.93161189712458	23.831147018844018
32	21.54202904604517	25.889131200335097	26.451997677058987	26.116842076560747
33	20.553444757921582	24.55396225626959	29.35080064304391	25.541792342764914
34	20.129525427327742	22.842330668680965	31.35094336119818	25.67720054279311
35	21.45692029307115	22.091667986519518	28.780682227586723	27.67072949282261
36	20.081372554138625	24.044063940053732	30.24661813089111	25.62794537491653
37	20.26974891467485	24.633153427237236	31.99456742765611	23.102530230431803
38	20.63600082846148	24.15916671162869	28.95832571879766	26.246506741112167
39	21.690200658243974	22.06028159568179	30.228981415891727	26.02053633018251
40	22.454438969714165	21.76213989047832	32.96151193059952	22.821909209207988
41	19.94254143902831	23.58446507089205	29.704173113640202	26.76882037643944
42	21.70366025653298	22.881085029271723	29.206458054496288	26.208796659699
43	21.6172751623419	22.70512398784691	29.7197212702844	25.95787957952679
44	20.59127087035448	22.769927312367688	29.177566330582817	27.461235486695013
45	20.302643708769104	21.61066139421713	29.155868529892782	28.930826367120986
46	22.23844722648153	22.866987260374188	29.765147414509798	25.12941809863449
47	19.798546943539886	22.435409882477984	32.80991740331861	24.95612577066353
48	19.666271581044477	20.94064027076999	31.716847181925615	27.67624096625992
49	20.498910178647158	20.755396747766547	32.33395816037459	26.4117349132117
50	19.131948735174863	20.74756465393458	32.39644086450071	27.72404574638984
51	19.31365331207645	20.348591992583298	29.28187821732262	31.055876478017634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	954.0
1	1514.5
2	2075.0
3	2562.0
4	3049.0
5	2563.0
6	2077.0
7	2147.0
8	2217.0
9	2177.0
10	2137.0
11	2382.5
12	2628.0
13	2816.0
14	3004.0
15	3327.5
16	3651.0
17	3396.5
18	3142.0
19	3827.0
20	4512.0
21	4888.5
22	5265.0
23	6142.0
24	7019.0
25	8034.5
26	12008.5
27	14967.0
28	18227.0
29	21487.0
30	23898.0
31	26309.0
32	29421.0
33	32533.0
34	36313.0
35	40093.0
36	45012.0
37	49931.0
38	52881.5
39	55832.0
40	62918.5
41	70005.0
42	79701.5
43	89398.0
44	105033.5
45	120669.0
46	144064.5
47	167460.0
48	187155.0
49	206850.0
50	199971.5
51	193093.0
52	162327.5
53	131562.0
54	112698.5
55	93835.0
56	84178.5
57	74522.0
58	70727.0
59	66932.0
60	66725.0
61	66518.0
62	60559.0
63	54600.0
64	45011.5
65	35423.0
66	28785.0
67	22147.0
68	18128.5
69	14110.0
70	12310.5
71	10511.0
72	8177.5
73	5844.0
74	5059.0
75	3399.0
76	2524.0
77	1677.5
78	831.0
79	608.0
80	385.0
81	283.0
82	181.0
83	122.0
84	63.0
85	34.0
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1723677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.564925797429144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.03892526635805	16.88280224176412
2	13.912702029608347	7.113543900573585
3	6.108338442427103	4.684776570786983
4	3.239112066114415	3.3123063847908965
5	1.942917743079321	2.483527396616567
6	1.2528805487052492	1.9217878956415202
7	0.8344825526924653	1.493343917718249
8	0.6051589160879149	1.2376674228352156
9	0.4649626142217507	1.0698061258041958
>10	4.003024251653793	24.952864977646083
>50	1.4519785441297275	24.88173143146592
>100	0.1327523739336413	5.6787487362435325
>500	0.007658790592963761	1.3078547747528058
>1k	0.004339981336012798	1.6834575607552207
>5k	7.658790592963761E-4	1.2957806626050303
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	7736	0.44880798432652985	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	6435	0.37332980599033344	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	5683	0.3297021425707949	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2493	0.14463266609695435	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2027	0.11759743849920838	TruSeq Adapter, Index 27 (95% over 23bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1897	0.11005542221657538	No Hit
TGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCTT	1784	0.10349966960167131	Illumina Single End Adapter 1 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1728	0.10025080104915247	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1603101973281537E-4	0.0	0.0	0.39015430385159167	0.0
2	1.1603101973281537E-4	0.0	0.0	1.4814840599485866	0.0
3	1.1603101973281537E-4	0.0	0.0	2.1282409639392994	0.0
4	1.1603101973281537E-4	0.0	0.0	2.943300862052461	0.0
5	1.7404652959922306E-4	0.0	0.0	5.0227507821941115	0.0
6	1.7404652959922306E-4	0.0	0.0	6.401141281110092	0.0
7	1.7404652959922306E-4	0.0	0.0	7.560523230280383	0.0
8	1.7404652959922306E-4	0.0	0.0	9.561014041493854	0.0
9	1.7404652959922306E-4	0.0	0.0	10.288702581748204	0.0
10	1.7404652959922306E-4	0.0	0.0	11.900257414817277	0.0
11	2.3206203946563074E-4	0.0	0.0	14.041783930515985	0.0
12	2.9007754933203843E-4	0.0	0.0	15.834753262937314	0.0
13	2.9007754933203843E-4	0.0	0.0	16.569809772944698	0.0
14	2.9007754933203843E-4	0.0	0.0	16.857334639842616	0.0
15	3.480930591984461E-4	0.0	0.0	17.42733702428007	0.0
16	4.641240789312615E-4	0.0	0.0	18.48826665320707	0.0
17	4.641240789312615E-4	0.0	0.0	19.671434961422587	0.0
18	4.641240789312615E-4	0.0	0.0	21.06624384963076	0.0
19	4.641240789312615E-4	0.0	0.0	21.89830229213478	0.0
20	4.641240789312615E-4	0.0	0.0	22.641597004543193	0.0
21	4.641240789312615E-4	0.0	0.0	23.480385246191716	0.0
22	4.641240789312615E-4	0.0	0.0	24.347136963595847	0.0
23	4.641240789312615E-4	0.0	0.0	25.19445348519473	0.0
24	4.641240789312615E-4	0.0	0.0	25.85049287076407	0.0
25	5.801550986640769E-4	0.0	0.0	26.436391504904922	0.0
26	5.801550986640769E-4	0.0	0.0	26.987306786596328	0.0
27	5.801550986640769E-4	0.0	0.0	27.481424884128522	0.0
28	5.801550986640769E-4	0.0	0.0	28.008843884324037	0.0
29	5.801550986640769E-4	0.0	0.0	28.5843577421988	0.0
30	5.801550986640769E-4	0.0	0.0	29.184064067687856	0.0
31	5.801550986640769E-4	0.0	0.0	29.756387072519967	0.0
32	5.801550986640769E-4	0.0	0.0	30.404594364257342	0.0
33	5.801550986640769E-4	0.0	0.0	31.006215201571987	0.0
34	5.801550986640769E-4	0.0	0.0	31.61822081515272	0.0
35	5.801550986640769E-4	0.0	0.0	32.25093796575577	0.0
36	5.801550986640769E-4	0.0	0.0	32.87466271232952	0.0
37	5.801550986640769E-4	0.0	0.0	33.476573627193495	0.0
38	5.801550986640769E-4	0.0	0.0	34.07123260332417	0.0
39	5.801550986640769E-4	0.0	0.0	34.66623967251405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	25	3.89162E-5	45.0	14
TAGACCG	20	7.034269E-4	45.0	1
CGACCTA	50	2.1827873E-11	45.0	33
TAACGGT	25	3.89162E-5	45.0	3
CCCGTTG	20	7.034269E-4	45.0	29
CGTAGCA	20	7.034269E-4	45.0	39
AACGCGT	20	7.034269E-4	45.0	34
CTCGCGA	20	7.034269E-4	45.0	37
CGTACGT	25	3.89162E-5	45.0	18
ATACGTA	40	6.8193913E-9	45.0	30
AATCGTC	20	7.034269E-4	45.0	18
TATAGCG	65	0.0	41.53846	1
CGTATGG	175	0.0	41.142857	2
CGTAAGG	360	0.0	40.625004	2
ACGTTAG	50	1.0822987E-9	40.5	1
TTACGCC	100	0.0	40.5	30
ATTGCGG	300	0.0	40.5	2
CTACGGG	490	0.0	40.408165	3
GCTACGA	130	0.0	39.80769	10
TCGTGCG	85	0.0	39.705883	45
>>END_MODULE