FastQCFastQC Report
Sat 14 Jan 2017
SRR2935092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4036227
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.17224006479318432No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG63300.15682963321934074No Hit
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT55130.13658795702025678TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC49510.12266406225417946TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT4300.040.29074
CGTTATT7600.038.4868431
TACCGGT3950.037.59493640
TTACGCG1750.037.2857171
GTATACG1050.036.428571
TATAACG1000.036.0000041
CGTTTTT48450.035.6656341
ACGGGTA4250.034.9411775
CGTATGG5550.034.8648642
TAACGGG15500.034.6935463
TACGGGA14950.034.6153834
GGGCGAT61200.034.5588267
CGAATAT3200.034.4531314
CGTAAGG7850.034.3949052
TTACGGG13850.034.2779773
AGTACGG7400.034.0540542
TATGGGC18450.033.7804874
AACGGGA17400.033.754
TAGGGAC18550.033.477095
CTTAACG1350.033.3333361
Produced by FastQC (version 0.11.5)