##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935092.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4036227
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.154721724025926	33.0	31.0	34.0	30.0	34.0
2	32.480265108974294	34.0	31.0	34.0	30.0	34.0
3	32.52894646411116	34.0	31.0	34.0	31.0	34.0
4	36.01578825967915	37.0	35.0	37.0	35.0	37.0
5	30.502193756694062	37.0	35.0	37.0	0.0	37.0
6	32.968816917383485	37.0	35.0	37.0	17.0	37.0
7	35.15404138568024	37.0	35.0	37.0	32.0	37.0
8	35.723136483651686	37.0	35.0	37.0	35.0	37.0
9	37.633637800847175	39.0	37.0	39.0	35.0	39.0
10	37.51054933233438	39.0	37.0	39.0	35.0	39.0
11	37.36348550267366	39.0	37.0	39.0	35.0	39.0
12	37.15793784640953	39.0	37.0	39.0	34.0	39.0
13	37.096051832565415	39.0	37.0	39.0	33.0	39.0
14	38.17422929879811	40.0	38.0	41.0	33.0	41.0
15	38.3291868866642	40.0	38.0	41.0	33.0	41.0
16	38.461810745530414	40.0	38.0	41.0	34.0	41.0
17	38.473164665912	40.0	38.0	41.0	34.0	41.0
18	38.42378885032978	40.0	37.0	41.0	34.0	41.0
19	38.39599061202455	40.0	37.0	41.0	34.0	41.0
20	38.29886995949435	40.0	37.0	41.0	34.0	41.0
21	38.25699520864411	40.0	37.0	41.0	34.0	41.0
22	38.27156029628661	40.0	37.0	41.0	34.0	41.0
23	38.20782998577632	40.0	37.0	41.0	34.0	41.0
24	38.178418855034664	40.0	37.0	41.0	34.0	41.0
25	38.105582763308405	40.0	36.0	41.0	34.0	41.0
26	38.04638316923206	40.0	36.0	41.0	34.0	41.0
27	38.00663391826079	40.0	36.0	41.0	34.0	41.0
28	37.94007101186331	40.0	36.0	41.0	34.0	41.0
29	37.92365543365128	40.0	36.0	41.0	34.0	41.0
30	37.813211694981476	40.0	36.0	41.0	33.0	41.0
31	33.214510977702695	39.0	33.0	41.0	9.0	41.0
32	34.13251633270379	39.0	33.0	41.0	16.0	41.0
33	36.066164266776866	38.0	34.0	41.0	29.0	41.0
34	36.85542413744321	38.0	35.0	41.0	31.0	41.0
35	36.982185838408	39.0	35.0	41.0	32.0	41.0
36	37.14499754349792	39.0	35.0	41.0	32.0	41.0
37	37.24352991048323	39.0	35.0	41.0	33.0	41.0
38	37.21005334932847	39.0	35.0	41.0	33.0	41.0
39	37.21189715048237	39.0	35.0	41.0	33.0	41.0
40	37.149213114128614	39.0	35.0	41.0	33.0	41.0
41	37.03760640816287	39.0	35.0	41.0	32.0	41.0
42	36.961216254685375	39.0	35.0	41.0	32.0	41.0
43	36.96561813792931	39.0	35.0	41.0	32.0	41.0
44	36.89698176044112	39.0	35.0	41.0	32.0	41.0
45	36.84151238272773	39.0	35.0	41.0	32.0	41.0
46	36.78600187749599	39.0	35.0	41.0	32.0	41.0
47	36.69037420343306	39.0	35.0	41.0	32.0	41.0
48	36.64038791673511	38.0	35.0	41.0	32.0	41.0
49	36.596539788272565	38.0	35.0	41.0	32.0	41.0
50	36.41047765648463	38.0	35.0	40.0	31.0	41.0
51	35.790402521959244	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	12.0
10	41.0
11	43.0
12	43.0
13	53.0
14	78.0
15	148.0
16	224.0
17	414.0
18	811.0
19	1405.0
20	2284.0
21	3619.0
22	5651.0
23	8791.0
24	13120.0
25	18463.0
26	24158.0
27	29804.0
28	36382.0
29	45350.0
30	60280.0
31	86206.0
32	126168.0
33	153907.0
34	292340.0
35	366434.0
36	465212.0
37	482216.0
38	681904.0
39	1129510.0
40	1143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04821160950561	21.718872600574745	25.771345367839814	11.461570422079829
2	32.68034726490854	23.592280612562178	25.668105386540454	18.05926673598883
3	32.54653417659611	23.725474310538033	26.275702531101448	17.452288981764404
4	28.84525573016582	25.833110972202505	25.983721926442687	19.337911371188984
5	22.80989151502133	39.080185529703854	21.211368934403342	16.898554020871472
6	28.166750779874373	32.955034491370284	23.565473398795458	15.312741329959886
7	82.76561253864067	5.432177129779866	7.182227362336162	4.619982969243306
8	84.54504664876382	4.0584436901096	7.090929226725851	4.305580434400741
9	78.4362970665426	6.311488427187073	9.058038608829484	6.194175897440853
10	45.18866258017698	23.480616922685467	15.704493330033223	15.626227167104329
11	31.6437355976262	23.213808341304887	24.10451642090497	21.037939640163945
12	28.139943566107657	20.90511757639003	29.350182732537096	21.60475612496522
13	25.880655374437563	20.89587627256842	31.610709704880325	21.612758648113697
14	21.327863868905293	23.4653551447924	32.93746858142518	22.269312404877127
15	21.008704416277876	23.65389756324409	33.16084551240552	22.176552508072515
16	26.169167393211527	23.599688520987545	28.432072824447186	21.799071261353735
17	26.6577920419243	23.952096846882	26.692056715343316	22.69805439585038
18	26.38312959107602	23.34687816121343	28.05426949475339	22.215722752957156
19	25.662159239309386	25.209211473983007	26.390983460543723	22.73764582616389
20	26.183636351473787	27.068299181388955	26.471380326230413	20.27668414090684
21	25.818567687099858	24.615984185230413	28.58516134003365	20.980286787636075
22	25.20425635129045	22.056886295047327	29.258190879749822	23.480666473912393
23	24.765926197907103	23.59614560826237	28.746376256835905	22.891551936994624
24	24.306734977988107	24.07429017248039	28.050577928347437	23.568396921184064
25	24.27323834858644	25.233318145882283	26.45688659235469	24.03655691317659
26	23.224461855093878	25.14541426931637	26.703973785418906	24.926150090170847
27	22.88077454513832	23.658704032255866	29.007585549573893	24.452935873031915
28	21.75801806984592	24.45244036076266	27.808842267791185	25.98069930160023
29	22.4668483710158	24.680128248485527	27.477765745088174	25.3752576354105
30	24.514404170033053	23.548675532867698	28.440224001276437	23.496696295822808
31	26.633809248092337	24.9790212493004	26.46652430599171	21.920645196615553
32	24.888416830867044	24.321005731342662	27.103059367077226	23.68751807071307
33	25.623013770038206	23.59443609093344	27.42296208810852	23.359588050919832
34	24.30737914393814	23.367218939866365	29.0363995880311	23.289002328164397
35	24.07805606572673	25.982235389634923	27.176122651178936	22.76358589345941
36	25.373696771762344	25.63569888413115	26.03684084170687	22.953763502399642
37	24.197301093323045	25.407961445181353	27.75579767936739	22.638939782128208
38	23.47974977621427	25.625144472796002	27.351038482226098	23.544067268763623
39	24.52986415283382	23.716233006716422	26.14662158496041	25.607281255489344
40	25.482610368544684	23.217549458937764	27.432401596837842	23.86743857567971
41	22.42695963334074	24.976890546542602	26.32408930419424	26.27206051592242
42	21.886504401263853	24.019684720408442	29.132950153695518	24.960860724632187
43	22.26849480963286	22.745797002993143	29.838460522661386	25.147247664712612
44	23.69153172009404	22.897919269654558	27.907176677624925	25.503372332626483
45	23.438399277344907	22.40344757616457	27.228795605400784	26.929357541089736
46	23.66113204237522	23.145824057963043	27.78337293715145	25.409670962510283
47	21.493389742450063	24.221928053105042	29.674173429789754	24.610508774655145
48	21.719070805482446	24.32920645939884	29.52063895316096	24.431083781957756
49	24.083655354369316	22.55794830171841	29.25078297132446	24.107613372587817
50	22.68487376948819	22.77027530909436	29.1417454964748	25.403105424942652
51	22.322926832410566	22.914791462422706	27.450339140984887	27.311942564181845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1088.0
1	2382.0
2	3676.0
3	9064.0
4	14452.0
5	10524.5
6	6597.0
7	6375.0
8	6153.0
9	6349.0
10	6545.0
11	6735.0
12	6925.0
13	7009.0
14	7093.0
15	7197.5
16	7302.0
17	7841.5
18	8381.0
19	8576.0
20	8771.0
21	9524.0
22	10277.0
23	11575.5
24	12874.0
25	15727.0
26	22966.0
27	27352.0
28	33327.5
29	39303.0
30	44477.5
31	49652.0
32	57421.5
33	65191.0
34	76782.5
35	88374.0
36	97368.5
37	106363.0
38	117241.0
39	128119.0
40	136656.5
41	145194.0
42	162339.5
43	179485.0
44	200768.5
45	222052.0
46	255767.5
47	289483.0
48	322711.0
49	355939.0
50	358761.5
51	361584.0
52	331765.5
53	301947.0
54	283680.0
55	265413.0
56	261399.0
57	257385.0
58	253747.0
59	250109.0
60	231783.5
61	213458.0
62	188892.5
63	164327.0
64	147610.0
65	130893.0
66	111425.5
67	91958.0
68	79503.5
69	67049.0
70	56530.0
71	46011.0
72	39795.0
73	33579.0
74	26566.5
75	14615.5
76	9677.0
77	7321.0
78	4965.0
79	3396.0
80	1827.0
81	1277.5
82	728.0
83	583.0
84	438.0
85	262.5
86	87.0
87	49.5
88	12.0
89	7.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4036227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.730011260055015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.10960956646487	15.935922148838074
2	12.193289232284615	5.543072030938738
3	4.922880226666778	3.356913689521142
4	2.4085673940017536	2.1898705594504495
5	1.4250127120965246	1.6195277495837772
6	0.8687055287175283	1.1847411869652877
7	0.6151816309307265	0.9788159778624097
8	0.4786732944601495	0.8704199498373465
9	0.36526355834964697	0.7472200314757714
>10	4.202615838012956	23.823216219095613
>50	2.1849123470393037	34.882979785272575
>100	0.21831175509056958	6.609629170032405
>500	0.0036720609919087526	0.5544923782450887
>1k	0.002937648793526423	1.1802647099804067
>5k	3.672060991908029E-4	0.5229144129008945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6952	0.17224006479318432	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	6330	0.15682963321934074	No Hit
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	5513	0.13658795702025678	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	4951	0.12266406225417946	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2298052116493945E-4	0.0	0.0	0.1436985580840721	0.0
2	2.477561346277105E-4	0.0	0.0	0.4548307119495509	0.0
3	2.477561346277105E-4	0.0	0.0	0.6858633074898909	0.0
4	2.477561346277105E-4	0.0	0.0	1.01711325948714	0.0
5	2.477561346277105E-4	0.0	0.0	1.9358921091405414	0.0
6	2.477561346277105E-4	0.0	0.0	2.774794380989969	0.0
7	2.477561346277105E-4	0.0	0.0	3.438062329001813	0.0
8	2.7253174809048155E-4	0.0	0.0	4.516173148834295	0.0
9	2.7253174809048155E-4	0.0	0.0	4.973085012314718	0.0
10	2.7253174809048155E-4	0.0	0.0	5.7817610357395655	0.0
11	2.7253174809048155E-4	0.0	0.0	7.011746366098834	0.0
12	3.220829750160236E-4	0.0	0.0	7.941005300246988	0.0
13	3.468585884787947E-4	0.0	0.0	8.33030451458751	0.0
14	3.468585884787947E-4	0.0	0.0	8.534678550041908	0.0
15	3.468585884787947E-4	0.0	0.0	8.823809959152445	0.0
16	3.468585884787947E-4	0.0	0.0	9.352942735876848	0.0
17	3.716342019415657E-4	0.0	0.0	9.988338118743073	0.0
18	3.716342019415657E-4	0.0	0.0	10.63347031770017	0.0
19	3.716342019415657E-4	0.0	0.0	11.1070809446545	0.0
20	3.964098154043368E-4	0.0	0.0	11.542041614606909	0.0
21	3.964098154043368E-4	0.0	0.0	12.062007414350084	0.0
22	3.964098154043368E-4	0.0	0.0	12.661403830854905	0.0
23	3.964098154043368E-4	0.0	0.0	13.182583635657757	0.0
24	4.459610423298789E-4	0.0	0.0	13.622548979529645	0.0
25	4.459610423298789E-4	0.0	0.0	14.02564821056893	0.0
26	4.707366557926499E-4	0.0	0.0	14.392104309296776	0.0
27	4.707366557926499E-4	0.0	0.0	14.787795631910694	0.0
28	4.707366557926499E-4	0.0	0.0	15.157893745817567	0.0
29	4.707366557926499E-4	0.0	0.0	15.588345254119751	0.0
30	4.707366557926499E-4	0.0	0.0	16.096592188695038	0.0
31	4.95512269255421E-4	0.0	0.0	16.50254061528254	0.0
32	4.95512269255421E-4	0.0	0.0	16.950731462824066	0.0
33	4.95512269255421E-4	0.0	0.0	17.423028982264874	0.0
34	4.95512269255421E-4	0.0	0.0	17.930433545982424	0.0
35	4.95512269255421E-4	0.0	0.0	18.45208408744107	0.0
36	6.193903365692762E-4	0.0	0.0	18.92524875335307	0.0
37	6.441659500320472E-4	0.0	0.0	19.421157432423893	0.0
38	6.441659500320472E-4	0.0	0.0	19.990649683479152	0.0
39	6.441659500320472E-4	0.0	0.0	20.838297746880937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	430	0.0	40.2907	4
CGTTATT	760	0.0	38.486843	1
TACCGGT	395	0.0	37.594936	40
TTACGCG	175	0.0	37.285717	1
GTATACG	105	0.0	36.42857	1
TATAACG	100	0.0	36.000004	1
CGTTTTT	4845	0.0	35.665634	1
ACGGGTA	425	0.0	34.941177	5
CGTATGG	555	0.0	34.864864	2
TAACGGG	1550	0.0	34.693546	3
TACGGGA	1495	0.0	34.615383	4
GGGCGAT	6120	0.0	34.558826	7
CGAATAT	320	0.0	34.45313	14
CGTAAGG	785	0.0	34.394905	2
TTACGGG	1385	0.0	34.277977	3
AGTACGG	740	0.0	34.054054	2
TATGGGC	1845	0.0	33.780487	4
AACGGGA	1740	0.0	33.75	4
TAGGGAC	1855	0.0	33.47709	5
CTTAACG	135	0.0	33.333336	1
>>END_MODULE