FastQCFastQC Report
Sat 14 Jan 2017
SRR2935091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4352824
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC118030.2711572992613531No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG97450.22387764816588035No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC89060.20460280498361522No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56990.1309264973727401No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC51410.11810723337309297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA403.4611912E-739.37500431
CGTAAGG5400.039.1666642
CGTTTTT47400.037.784811
TACGGGT6300.036.428574
ACGGGAG33550.036.3487325
TAATACG6350.036.141734
CGCAATA504.8843503E-836.013
TACGGGA18850.035.8090174
ACAACGA19200.035.62513
TTACGGG17350.035.4034583
TACGGCT21100.034.9763037
CGTTAGG6050.034.958682
TGTTACG2200.034.7727281
CGTTATT8350.034.2215581
AGCGACT23350.034.2077119
CACGACC17200.034.01162727
CACAACG20400.033.9705912
TAGTGCG3450.033.9130441
AACACGT18350.033.8419641
GGGCGAT67900.033.7997067
Produced by FastQC (version 0.11.5)