##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935090.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2104074
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.983806177919597	33.0	31.0	34.0	30.0	34.0
2	32.30554486201531	34.0	31.0	34.0	30.0	34.0
3	32.374899837173025	34.0	31.0	34.0	30.0	34.0
4	35.89917322299501	37.0	35.0	37.0	35.0	37.0
5	30.352164895341133	37.0	35.0	37.0	0.0	37.0
6	32.82576183157056	37.0	35.0	37.0	17.0	37.0
7	35.034833375632225	37.0	35.0	37.0	32.0	37.0
8	35.6053627391432	37.0	35.0	37.0	35.0	37.0
9	37.47689529930982	39.0	37.0	39.0	35.0	39.0
10	37.390334655530175	39.0	37.0	39.0	35.0	39.0
11	37.291335285736146	39.0	37.0	39.0	34.0	39.0
12	37.133471066131705	39.0	37.0	39.0	34.0	39.0
13	37.07579296165439	39.0	37.0	39.0	33.0	39.0
14	38.17462361114676	40.0	37.0	41.0	33.0	41.0
15	38.30612611533625	40.0	38.0	41.0	34.0	41.0
16	38.4028251858062	40.0	38.0	41.0	34.0	41.0
17	38.40111136775608	40.0	38.0	41.0	34.0	41.0
18	38.379715732431464	40.0	37.0	41.0	34.0	41.0
19	38.37828517438075	40.0	37.0	41.0	34.0	41.0
20	38.29284283727664	40.0	37.0	41.0	34.0	41.0
21	38.242546127179935	40.0	37.0	41.0	34.0	41.0
22	38.26677959045167	40.0	37.0	41.0	34.0	41.0
23	38.18872767782882	40.0	37.0	41.0	34.0	41.0
24	38.15896066393102	40.0	37.0	41.0	34.0	41.0
25	38.08508826210485	40.0	37.0	41.0	34.0	41.0
26	38.03860558136263	40.0	37.0	41.0	34.0	41.0
27	38.01588109543676	40.0	37.0	41.0	34.0	41.0
28	37.96783240513404	40.0	36.0	41.0	34.0	41.0
29	37.987532282609834	40.0	36.0	41.0	34.0	41.0
30	37.86983585178088	40.0	36.0	41.0	33.0	41.0
31	33.253034351453415	39.0	33.0	41.0	9.0	41.0
32	34.17267548574812	39.0	33.0	41.0	16.0	41.0
33	36.13903693501274	39.0	34.0	41.0	30.0	41.0
34	37.006871906596444	39.0	35.0	41.0	31.0	41.0
35	37.168954133742446	39.0	35.0	41.0	32.0	41.0
36	37.315980806758695	39.0	35.0	41.0	33.0	41.0
37	37.44160661649733	39.0	35.0	41.0	33.0	41.0
38	37.40331138543606	39.0	35.0	41.0	33.0	41.0
39	37.39264636129718	39.0	35.0	41.0	33.0	41.0
40	37.35943507690319	39.0	35.0	41.0	33.0	41.0
41	37.264837643542954	39.0	35.0	41.0	33.0	41.0
42	37.17187608420616	39.0	35.0	41.0	33.0	41.0
43	37.14706184288195	39.0	35.0	41.0	33.0	41.0
44	37.09340926222177	39.0	35.0	41.0	33.0	41.0
45	37.06112189970505	39.0	35.0	41.0	33.0	41.0
46	37.003408625362034	39.0	35.0	41.0	32.0	41.0
47	36.8963843476988	39.0	35.0	41.0	32.0	41.0
48	36.89425894716631	39.0	35.0	41.0	32.0	41.0
49	36.88336959631648	39.0	35.0	41.0	32.0	41.0
50	36.69685049099984	39.0	35.0	41.0	32.0	41.0
51	36.076669356686125	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	13.0
11	13.0
12	19.0
13	27.0
14	33.0
15	54.0
16	104.0
17	202.0
18	336.0
19	628.0
20	1044.0
21	1650.0
22	2784.0
23	4362.0
24	6432.0
25	8226.0
26	10652.0
27	13382.0
28	17091.0
29	22320.0
30	30074.0
31	43315.0
32	65941.0
33	79091.0
34	145714.0
35	198901.0
36	251179.0
37	254982.0
38	380741.0
39	564242.0
40	508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.810709129051546	20.747321624619666	24.745279871335324	11.696689374993465
2	30.72971768103213	24.233415744883498	25.888300506541118	19.148566067543253
3	31.241201592719648	24.31725310041377	26.000701496240154	18.440843810626433
4	27.98176299882989	25.505661873109027	25.890914483045748	20.621660645015336
5	22.783656848570917	38.27384398077254	21.49330299219514	17.449196178461403
6	26.67876700154082	32.034947440061515	24.378895419077466	16.907390139320196
7	78.43569190057003	5.7864884980281115	9.717576473070814	6.060243128331038
8	78.0164100692276	5.059898083432427	9.689345526820825	7.234346320519146
9	71.53061156594302	6.998423059265026	12.361114675624526	9.109850699167426
10	39.80330539705352	24.194396204696222	19.254360825712403	16.74793757253785
11	29.245929563313837	22.883938492657578	25.085809719620126	22.784322224408456
12	25.028017075445064	21.029821194501714	31.58829014568879	22.35387158436443
13	23.256501434835467	23.310444404521892	33.17806312895839	20.25499103168425
14	19.277363818953138	25.820384644266316	31.867462836383133	23.034788700397417
15	17.329666161931566	24.449995580003364	35.81770413017793	22.402634127887136
16	20.85573035929345	25.328719427168433	30.164575960731423	23.650974252806698
17	21.0311519461768	25.167080625491312	27.72668641882367	26.07508100950822
18	22.482574282083235	23.41718969960182	30.422646732006577	23.677589286308372
19	21.537550485391673	25.99846773449983	27.981240203528962	24.48274157657953
20	22.743686771472866	26.934936698994427	28.81733247024582	21.50404405928689
21	22.053359340023214	26.748964152401484	29.915582816954156	21.28209369062115
22	21.49929137473302	22.97794659313313	29.135762335355125	26.386999696778723
23	20.56510369882428	25.158478266448803	29.197642288246517	25.078775746480396
24	21.712211642746404	23.74365160160717	29.308569945733847	25.23556680991258
25	21.337557519364815	26.80480819590946	26.68613366259932	25.17150062212641
26	20.382553085110125	25.139657635615475	28.37096033694632	26.106828942328075
27	21.265316714145985	24.44063279143224	29.141655664201927	25.152394830219848
28	18.02964154302558	24.427705489445714	29.20657733520779	28.336075632320917
29	19.76988451927071	22.58969979192747	29.26460761361055	28.375808075191273
30	21.062899878996653	23.314008917937297	29.604424559212273	26.018666643853784
31	23.278601418010965	24.863193975116847	25.049926951238405	26.808277655633784
32	20.501940521103347	24.198626093949166	27.39618473494754	27.903248649999952
33	20.48202677282263	23.625404809906875	26.3218879183907	29.570680498879792
34	18.33105679743203	22.230301785963803	29.025690161087493	30.412951255516678
35	18.283577478738867	23.49289996454497	30.615130456438315	27.60839210027784
36	20.01825981405597	25.64320456409803	28.579080393560304	25.7594552282857
37	18.80718073603875	24.639057371556326	28.828168591028643	27.725593301376282
38	19.018104876539514	25.360609940524903	26.648397347241588	28.972887835693996
39	20.931583204773215	23.033172787649104	26.635612625791676	29.399631381786
40	21.98677422942349	20.814191896292623	30.489849691598298	26.70918418268559
41	18.561847159367968	23.620794705889622	29.091752476386283	28.725605658356123
42	20.1079429715875	23.161447743758064	31.254984377925872	25.475624906728566
43	21.053489563579987	22.554815087302064	30.22384193711818	26.16785341199977
44	20.714005305897036	21.491972240520056	27.924493149955754	29.86952930362715
45	20.519525453952664	20.68406339320765	27.936469915031502	30.859941237808176
46	21.89732870611965	21.290600995972575	28.754359399907038	28.05771089800074
47	17.48588690321728	22.804996402217792	32.40171210708368	27.307404587481237
48	18.303015958564195	22.505292114250736	30.91659323769031	28.27509868949476
49	20.82379231909144	20.442627017871047	31.893032279282952	26.840548383754566
50	19.038208732202385	20.95672490606319	30.895158630352356	29.109907731382073
51	18.56108672983935	20.241731041778948	28.19891315609622	32.99826907228548
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1183.0
1	1633.5
2	2084.0
3	2741.5
4	3399.0
5	2800.5
6	2202.0
7	2256.0
8	2310.0
9	2460.0
10	2610.0
11	2792.0
12	2974.0
13	3183.0
14	3392.0
15	3507.0
16	3622.0
17	3694.0
18	3766.0
19	3866.5
20	3967.0
21	4368.0
22	4769.0
23	5471.0
24	6173.0
25	7543.5
26	11418.0
27	13922.0
28	16093.0
29	18264.0
30	21458.5
31	24653.0
32	28879.5
33	33106.0
34	39462.0
35	45818.0
36	48749.5
37	51681.0
38	54834.0
39	57987.0
40	67349.0
41	76711.0
42	90040.0
43	103369.0
44	120779.0
45	138189.0
46	168803.5
47	199418.0
48	227028.0
49	254638.0
50	248818.5
51	242999.0
52	208476.5
53	173954.0
54	153758.5
55	133563.0
56	121775.5
57	109988.0
58	99655.0
59	89322.0
60	81900.0
61	74478.0
62	67559.0
63	60640.0
64	55247.0
65	49854.0
66	42063.0
67	34272.0
68	29044.0
69	23816.0
70	20106.0
71	16396.0
72	13684.0
73	10972.0
74	8944.5
75	5513.0
76	4109.0
77	3183.0
78	2257.0
79	1587.5
80	918.0
81	569.5
82	221.0
83	223.0
84	225.0
85	130.5
86	36.0
87	20.5
88	5.0
89	3.5
90	2.0
91	3.0
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2104074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.743149765969225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.30519915482054	15.268079742633816
2	14.025639620749383	6.660257241579287
3	6.502910423501755	4.631987282996537
4	3.5437760579055024	3.3656162271962553
5	2.1077633029456333	2.502246988652607
6	1.4191606642605599	2.0217206516106505
7	0.9894776242810889	1.6445320796366922
8	0.7581586791295475	1.44008600519538
9	0.5362258383633007	1.1458521349778028
>10	3.9237157327107286	20.6090754709994
>50	1.6161693349900363	27.04714021260345
>100	0.2562783769227648	8.740607429573597
>500	0.009675118043859024	1.5628822227692538
>1k	0.005175063139738548	2.0405349301279925
>5k	6.750082356180714E-4	1.3193813794473512
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	9583	0.4554497607973864	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	7813	0.3713272441938829	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	7301	0.3469934992780672	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	3035	0.14424397620996218	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	2373	0.11278120446334113	No Hit
ACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2331	0.11078507695071561	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	2274	0.10807604675500956	No Hit
TGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCTT	2253	0.10707798299869681	TruSeq Adapter, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4258053661610762E-4	0.0	0.0	0.3879616401324288	0.0
2	1.4258053661610762E-4	0.0	0.0	1.4983788592986749	0.0
3	1.4258053661610762E-4	0.0	0.0	2.160950612953727	0.0
4	1.4258053661610762E-4	0.0	0.0	2.9955220206133433	0.0
5	1.9010738215481014E-4	0.0	0.0	5.131758673886945	0.0
6	1.9010738215481014E-4	0.0	0.0	6.519257402543827	0.0
7	1.9010738215481014E-4	0.0	0.0	7.785752782459172	0.0
8	1.9010738215481014E-4	0.0	0.0	9.872276355299292	0.0
9	1.9010738215481014E-4	0.0	0.0	10.589646561860468	0.0
10	1.9010738215481014E-4	0.0	0.0	12.188972441083346	0.0
11	2.376342276935127E-4	0.0	0.0	14.466696513525665	0.0
12	2.8516107323221524E-4	0.0	0.0	16.408453314854896	0.0
13	2.8516107323221524E-4	0.0	0.0	17.151678125389125	0.0
14	2.8516107323221524E-4	0.0	0.0	17.457703483812832	0.0
15	3.3268791877091777E-4	0.0	0.0	18.053452492640467	0.0
16	3.3268791877091777E-4	0.0	0.0	19.10869104413628	0.0
17	3.3268791877091777E-4	0.0	0.0	20.39139307838032	0.0
18	3.802147643096203E-4	0.0	0.0	21.73302840109236	0.0
19	3.802147643096203E-4	0.0	0.0	22.60690451001248	0.0
20	3.802147643096203E-4	0.0	0.0	23.363104149378778	0.0
21	3.802147643096203E-4	0.0	0.0	24.227427362345622	0.0
22	3.802147643096203E-4	0.0	0.0	25.137138712801928	0.0
23	3.802147643096203E-4	0.0	0.0	25.97983721104866	0.0
24	3.802147643096203E-4	0.0	0.0	26.67178055524663	0.0
25	3.802147643096203E-4	0.0	0.0	27.3044103962123	0.0
26	3.802147643096203E-4	0.0	0.0	27.857480297746182	0.0
27	3.802147643096203E-4	0.0	0.0	28.45798199112769	0.0
28	4.277416098483228E-4	0.0	0.0	29.002402006773526	0.0
29	4.277416098483228E-4	0.0	0.0	29.626952283997618	0.0
30	4.277416098483228E-4	0.0	0.0	30.337288517419065	0.0
31	4.277416098483228E-4	0.0	0.0	30.893305083376344	0.0
32	4.277416098483228E-4	0.0	0.0	31.530640082050347	0.0
33	4.277416098483228E-4	0.0	0.0	32.146540473386395	0.0
34	4.277416098483228E-4	0.0	0.0	32.79019654251704	0.0
35	4.277416098483228E-4	0.0	0.0	33.509135134980994	0.0
36	4.277416098483228E-4	0.0	0.0	34.11006456997235	0.0
37	4.752684553870254E-4	0.0	0.0	34.75001354515098	0.0
38	4.752684553870254E-4	0.0	0.0	35.42147281892177	0.0
39	4.752684553870254E-4	0.0	0.0	36.27980764935074	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACA	20	7.0346234E-4	45.000004	23
TAGTCGA	20	7.0346234E-4	45.000004	38
CGTGTTA	95	0.0	42.63158	10
CTAACGG	175	0.0	42.42857	2
TATACGC	225	0.0	40.0	26
ACTATCG	130	0.0	39.807693	31
TAAGACG	120	0.0	39.375004	1
CCGTATC	195	0.0	39.23077	29
ACAACGA	510	0.0	38.82353	13
TAGCACG	175	0.0	38.571426	1
CCGTTTA	35	6.2507643E-6	38.571426	25
GTCGGTC	35	6.2507643E-6	38.571426	42
TAAACGG	240	0.0	38.437504	2
CGTATCG	195	0.0	38.076923	30
TTACGGG	835	0.0	37.724552	3
CGCATGG	305	0.0	37.62295	2
CGTGAAT	30	1.14024406E-4	37.500004	17
CGCTAAA	30	1.14024406E-4	37.500004	34
TCGAACT	30	1.14024406E-4	37.500004	25
CACAACG	530	0.0	37.35849	12
>>END_MODULE