##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935089.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3225275
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.105947864910746	33.0	31.0	34.0	30.0	34.0
2	32.420539023804174	34.0	31.0	34.0	30.0	34.0
3	32.47526520994334	34.0	31.0	34.0	30.0	34.0
4	35.97551247568037	37.0	35.0	37.0	35.0	37.0
5	30.43678911100604	37.0	35.0	37.0	0.0	37.0
6	32.91202920681182	37.0	35.0	37.0	17.0	37.0
7	35.11416855927014	37.0	35.0	37.0	32.0	37.0
8	35.67288339753974	37.0	35.0	37.0	35.0	37.0
9	37.593416375347836	39.0	37.0	39.0	35.0	39.0
10	37.46146204587206	39.0	37.0	39.0	35.0	39.0
11	37.31256838564153	39.0	37.0	39.0	34.0	39.0
12	37.057083814558446	39.0	37.0	39.0	34.0	39.0
13	36.92683507607878	39.0	37.0	39.0	33.0	39.0
14	37.81471068358512	40.0	37.0	41.0	33.0	41.0
15	38.002855260404154	40.0	37.0	41.0	33.0	41.0
16	38.20763965863376	40.0	37.0	41.0	33.0	41.0
17	38.25613660850625	40.0	37.0	41.0	34.0	41.0
18	38.23106184743936	40.0	37.0	41.0	34.0	41.0
19	38.20802443202518	40.0	37.0	41.0	34.0	41.0
20	38.07496415034377	40.0	36.0	41.0	33.0	41.0
21	38.0656756400617	40.0	36.0	41.0	33.0	41.0
22	38.11622450798769	40.0	36.0	41.0	34.0	41.0
23	38.06777561603274	40.0	36.0	41.0	34.0	41.0
24	38.04253962840378	40.0	36.0	41.0	34.0	41.0
25	37.97939586546884	40.0	36.0	41.0	34.0	41.0
26	37.91671903946175	40.0	36.0	41.0	34.0	41.0
27	37.86133430482672	40.0	36.0	41.0	34.0	41.0
28	37.81133329716071	40.0	36.0	41.0	34.0	41.0
29	37.77385711295936	40.0	36.0	41.0	33.0	41.0
30	37.66029036283728	40.0	35.0	41.0	33.0	41.0
31	33.09582593732318	38.0	33.0	41.0	9.0	41.0
32	33.9917176054755	38.0	33.0	41.0	16.0	41.0
33	35.91588097139004	38.0	34.0	41.0	29.0	41.0
34	36.7280110998287	38.0	35.0	41.0	31.0	41.0
35	36.906835231104324	39.0	35.0	41.0	31.0	41.0
36	37.07738688949004	39.0	35.0	41.0	32.0	41.0
37	37.19331932935951	39.0	35.0	41.0	32.0	41.0
38	37.17580423374751	39.0	35.0	41.0	32.0	41.0
39	37.15623070900931	39.0	35.0	41.0	32.0	41.0
40	37.102556216136605	39.0	35.0	41.0	32.0	41.0
41	37.034592399097754	39.0	35.0	41.0	32.0	41.0
42	36.98849493454046	39.0	35.0	41.0	32.0	41.0
43	36.999625148243176	39.0	35.0	41.0	32.0	41.0
44	36.951885653161355	39.0	35.0	41.0	32.0	41.0
45	36.92577160087124	39.0	35.0	41.0	32.0	41.0
46	36.91012797358365	39.0	35.0	41.0	32.0	41.0
47	36.81372224073916	39.0	35.0	41.0	32.0	41.0
48	36.78785312880297	39.0	35.0	41.0	32.0	41.0
49	36.77309531745355	39.0	35.0	41.0	32.0	41.0
50	36.57046081341901	39.0	35.0	41.0	32.0	41.0
51	35.930388571517156	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	27.0
10	51.0
11	48.0
12	39.0
13	58.0
14	68.0
15	111.0
16	190.0
17	347.0
18	709.0
19	1234.0
20	2004.0
21	3413.0
22	5299.0
23	8120.0
24	11654.0
25	15120.0
26	18858.0
27	23388.0
28	28800.0
29	37227.0
30	51051.0
31	75367.0
32	108544.0
33	132838.0
34	251955.0
35	289366.0
36	356476.0
37	367293.0
38	534522.0
39	900155.0
40	934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.82361969134415	20.85276449295021	24.324902527691435	9.998713288014201
2	33.1965801365775	24.077760811093626	24.87282479788545	17.85283425444342
3	32.605994837649504	24.544325677655394	25.237072807745076	17.612606676950026
4	29.493516056770353	25.231398872964323	25.79575385044686	19.479331219818466
5	23.381944175302884	38.93202905178628	21.00685988016526	16.67916689274558
6	27.80779933494043	34.01905263892226	23.694444659757696	14.47870336637961
7	80.71978358434552	6.17879095580997	8.288998612521413	4.8124268473230964
8	82.14580152080056	5.345931742254536	7.4880126500841016	5.0202540868608105
9	76.48284254830983	6.512746975064141	9.647177372472115	7.357233104153909
10	44.48240847679655	22.740882560401825	17.295920502902852	15.480788459898767
11	33.90222539163327	24.57005991737139	23.148475711373447	18.379238979621892
12	30.3652556758726	22.484253280728	27.53681469022021	19.613676353179184
13	28.46123198797002	21.922006650595684	30.85178783204533	18.764973529388968
14	23.08835680678392	24.485788033578533	32.26205517358985	20.1637999860477
15	22.574881211679624	24.785235367526802	33.65033989349745	18.98954352729612
16	27.957895063211662	24.41661563742627	27.672120981931776	19.953368317430296
17	28.227143421878754	23.98725690057437	26.3079582361194	21.477641441427476
18	28.483617676012123	23.36678267744611	28.448426878328203	19.701172768213564
19	26.484346296052273	25.770577702676515	26.336172884482718	21.40890311678849
20	24.403841532892546	29.427630202075793	27.092666516808645	19.07586174822302
21	24.775530768694143	26.297106448287355	30.31710474300641	18.61025804001209
22	22.91528629341684	23.55848726077621	31.476013673252666	22.05021277255428
23	22.3487299532598	25.38871879142089	30.717225662927966	21.545325592391347
24	23.273767353171433	26.38382773561944	28.04892606056848	22.293478850640643
25	23.679531202765656	26.530729937757247	26.73954934075389	23.05018951872321
26	21.62122609699948	26.31874801373526	27.20775747804451	24.85226841122075
27	22.10121617536489	25.760780088519585	29.594902760229747	22.54310097588578
28	20.469479346722373	26.56151801009216	27.94003612095093	25.028966522234537
29	23.224159180224945	27.520040926742677	26.884436210865744	22.37136368216664
30	25.129175031586453	25.530350125183126	28.00973560394075	21.330739239289674
31	26.611095177930565	26.189983799831023	26.28510747145592	20.913813550782493
32	27.33949818232554	26.155599134957484	25.45913139189682	21.045771290820163
33	25.85782607684616	25.83190580648162	26.35511700552666	21.95515111114556
34	22.777127531760858	25.835099332615048	28.931982544124146	22.455790591499948
35	22.586477122105865	28.353148181162847	27.46094519072017	21.599429506011113
36	25.57800497631985	27.737665780437325	26.0442597918007	20.640069451442127
37	23.839703591166643	27.19321608234957	26.90297726550449	22.064103060979296
38	25.234065312260196	26.119385168706543	26.610103014471637	22.036446504561628
39	24.2684732309648	25.525544333428936	26.075636961189357	24.13034547441691
40	23.866181953476836	24.346358062490793	28.65622311275783	23.131236871274545
41	21.520273465053368	24.807621055568905	27.775523017417118	25.89658246196061
42	22.72144235762842	24.13853082295308	30.33363046561921	22.80639635379929
43	23.152413360101075	23.953306307214113	29.577663920130842	23.316616412553966
44	23.841129826138857	24.275914456906776	27.740022168652285	24.142933548302086
45	21.8947841656913	23.983970359116665	27.08736464332499	27.03388083186705
46	22.960925812527613	24.664873537915373	28.08476796552232	24.289432684034693
47	20.697118850330593	25.459565463410094	31.15228933966871	22.6910263465906
48	21.28571982234073	26.67403554735643	28.764430939997364	23.275813690305476
49	24.555921588081635	23.62266783452574	29.13925169171621	22.682158885676415
50	21.78279371526459	25.195060886281013	28.16116455185992	24.860980846594476
51	21.30342373906101	24.981776747719188	26.683771151297176	27.031028361922626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	876.0
1	2048.0
2	3220.0
3	4375.5
4	5531.0
5	4599.5
6	3668.0
7	3545.5
8	3423.0
9	3649.0
10	3875.0
11	4233.5
12	4592.0
13	4955.0
14	5318.0
15	5595.5
16	5873.0
17	6103.5
18	6334.0
19	6704.0
20	7074.0
21	7690.5
22	8307.0
23	9942.0
24	11577.0
25	13157.0
26	18699.5
27	22662.0
28	26691.0
29	30720.0
30	35928.5
31	41137.0
32	48381.5
33	55626.0
34	66330.0
35	77034.0
36	84685.5
37	92337.0
38	102261.5
39	112186.0
40	125192.0
41	138198.0
42	149797.0
43	161396.0
44	182113.0
45	202830.0
46	243150.5
47	283471.0
48	305799.5
49	328128.0
50	326805.0
51	325482.0
52	297946.0
53	270410.0
54	246294.5
55	222179.0
56	206647.0
57	191115.0
58	180912.0
59	170709.0
60	145117.5
61	119526.0
62	105851.0
63	92176.0
64	80255.5
65	68335.0
66	59602.0
67	50869.0
68	42351.5
69	33834.0
70	27555.0
71	21276.0
72	17046.5
73	12817.0
74	10323.0
75	6194.0
76	4559.0
77	3292.0
78	2025.0
79	1493.5
80	962.0
81	748.0
82	534.0
83	424.0
84	314.0
85	204.5
86	95.0
87	75.5
88	56.0
89	46.0
90	36.0
91	18.5
92	1.0
93	1.5
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3225275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.220427687859207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.71641765998415	15.046877618945501
2	13.115956899981274	5.828843437062239
3	5.57845707322504	3.7186710601627113
4	2.8502777875332352	2.5333756587277434
5	1.7357018878240236	1.9284019143037214
6	1.0891179273542433	1.4520399688995635
7	0.7606064503981337	1.1830700440993702
8	0.5689785361383143	1.011435713456432
9	0.44353622380736224	0.8870008129251866
>10	3.787632774908277	19.193595766742316
>50	1.8405167385422114	29.97267945683657
>100	0.5036246389558227	14.269696133305985
>500	0.005536880123818196	0.8223448480207136
>1k	0.003005734924358449	1.2973883752564117
>5k	6.327862998649367E-4	0.8545791912555171
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	7532	0.2335304741456155	No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	6607	0.20485074916092427	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGC	5116	0.15862213299641117	No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	5056	0.1567618265109177	TruSeq Adapter, Index 19 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	4781	0.1482354217857392	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCG	4015	0.12448550898760571	No Hit
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	3729	0.11561804807342009	TruSeq Adapter, Index 13 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	3445	0.10681259737541761	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.201021618311617E-5	0.0	0.0	0.1643580779933494	0.0
2	6.201021618311617E-5	0.0	0.0	0.6156994364821604	0.0
3	6.201021618311617E-5	0.0	0.0	0.9071474525428064	0.0
4	9.301532427467425E-5	0.0	0.0	1.3318554231809692	0.0
5	9.301532427467425E-5	0.0	0.0	2.4477912736123275	0.0
6	9.301532427467425E-5	0.0	0.0	3.2067343094774863	0.0
7	9.301532427467425E-5	0.0	0.0	3.893497453705498	0.0
8	9.301532427467425E-5	0.0	0.0	5.076063281425615	0.0
9	9.301532427467425E-5	0.0	0.0	5.478323553805489	0.0
10	9.301532427467425E-5	0.0	0.0	6.463417848090473	0.0
11	9.301532427467425E-5	0.0	0.0	7.852632721240824	0.0
12	9.301532427467425E-5	0.0	0.0	9.086884064149569	0.0
13	9.301532427467425E-5	0.0	0.0	9.527807706319615	0.0
14	9.301532427467425E-5	0.0	0.0	9.736875150180992	0.0
15	9.301532427467425E-5	0.0	0.0	10.106114982443357	0.0
16	1.2402043236623233E-4	0.0	0.0	10.749563990667463	0.0
17	1.2402043236623233E-4	0.0	0.0	11.493128492919208	0.0
18	1.2402043236623233E-4	0.0	0.0	12.298145119408423	0.0
19	1.5502554045779043E-4	0.0	0.0	12.911612188107991	0.0
20	1.5502554045779043E-4	0.0	0.0	13.459100386788723	0.0
21	1.5502554045779043E-4	0.0	0.0	14.09144956631605	0.0
22	1.5502554045779043E-4	0.0	0.0	14.751424297152957	0.0
23	1.5502554045779043E-4	0.0	0.0	15.335436513165543	0.0
24	1.5502554045779043E-4	0.0	0.0	15.83244839587322	0.0
25	1.5502554045779043E-4	0.0	0.0	16.297835068327508	0.0
26	1.5502554045779043E-4	0.0	0.0	16.737580516390075	0.0
27	1.5502554045779043E-4	0.0	0.0	17.236638736231793	0.0
28	1.860306485493485E-4	0.0	0.0	17.690150452287014	0.0
29	1.860306485493485E-4	0.0	0.0	18.227686011270357	0.0
30	1.860306485493485E-4	0.0	0.0	18.881738766461776	0.0
31	1.860306485493485E-4	0.0	0.0	19.382564277464713	0.0
32	1.860306485493485E-4	0.0	0.0	19.912379564533257	0.0
33	1.860306485493485E-4	0.0	0.0	20.481416313337622	0.0
34	1.860306485493485E-4	0.0	0.0	21.08031098123416	0.0
35	2.1703575664090658E-4	0.0	0.0	21.708691506925767	0.0
36	2.4804086473246466E-4	0.0	0.0	22.265946314655338	0.0
37	2.7904597282402274E-4	0.0	0.0	22.862329568796458	0.0
38	3.1005108091558087E-4	0.0	0.0	23.611319964964228	0.0
39	3.1005108091558087E-4	0.0	0.0	24.920882715427368	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	40	3.4608092E-7	39.375004	1
ACAACGA	595	0.0	39.327732	13
TACGGGA	770	0.0	38.57143	4
ATTACGC	30	1.14037895E-4	37.500004	28
CGATCAT	30	1.14037895E-4	37.500004	10
CACAACG	640	0.0	37.26563	12
ACGCATA	270	0.0	36.666668	25
TTACGGG	865	0.0	36.156067	3
TACGGGT	355	0.0	36.126762	4
CGAAACG	25	0.0021076715	36.000004	2
CCGTAAC	25	0.0021076715	36.000004	6
ACGTCGT	25	0.0021076715	36.000004	45
AATCGTC	25	0.0021076715	36.000004	10
AACGAGC	815	0.0	35.889572	15
TTGTACG	145	0.0	35.68966	1
TATAGCG	230	0.0	35.217392	1
AGCGACT	820	0.0	34.84756	19
ATGGGCG	1170	0.0	34.230766	5
TGGGCGA	1950	0.0	33.80769	6
CGTAATC	40	1.5614378E-5	33.750004	23
>>END_MODULE