##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935088.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3629365
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95628353720279	33.0	31.0	34.0	30.0	34.0
2	32.291100509317744	34.0	31.0	34.0	30.0	34.0
3	32.33855536712345	34.0	31.0	34.0	30.0	34.0
4	35.87099010432954	37.0	35.0	37.0	35.0	37.0
5	30.347940479946217	37.0	35.0	37.0	0.0	37.0
6	32.830457394062044	37.0	35.0	37.0	17.0	37.0
7	35.08575632376463	37.0	35.0	37.0	32.0	37.0
8	35.684562726537564	37.0	35.0	37.0	35.0	37.0
9	37.575947032056575	39.0	37.0	39.0	35.0	39.0
10	37.3392136089922	39.0	37.0	39.0	34.0	39.0
11	37.24703660282171	39.0	37.0	39.0	34.0	39.0
12	37.26052160639671	39.0	37.0	39.0	34.0	39.0
13	37.3171811046836	39.0	37.0	39.0	34.0	39.0
14	38.543069930965885	40.0	38.0	41.0	34.0	41.0
15	38.61674673117749	40.0	38.0	41.0	35.0	41.0
16	38.65622967103061	40.0	38.0	41.0	35.0	41.0
17	38.64611385187216	40.0	38.0	41.0	35.0	41.0
18	38.59620539681184	40.0	38.0	41.0	35.0	41.0
19	38.59551574448974	40.0	38.0	41.0	34.0	41.0
20	38.540373040463	40.0	38.0	41.0	34.0	41.0
21	38.499934286025244	40.0	38.0	41.0	34.0	41.0
22	38.5109742337847	40.0	38.0	41.0	34.0	41.0
23	38.43339620016174	40.0	38.0	41.0	34.0	41.0
24	38.42290621086609	40.0	38.0	41.0	34.0	41.0
25	38.36481919013381	40.0	38.0	41.0	34.0	41.0
26	38.313393665283044	40.0	38.0	41.0	34.0	41.0
27	38.275827038614196	40.0	38.0	41.0	34.0	41.0
28	38.23253516799771	40.0	37.0	41.0	34.0	41.0
29	38.259532452646674	40.0	38.0	41.0	34.0	41.0
30	38.111550092096	40.0	37.0	41.0	34.0	41.0
31	33.52894762582435	39.0	34.0	41.0	9.0	41.0
32	34.48605554966227	39.0	34.0	41.0	16.0	41.0
33	36.47961695778738	39.0	34.0	41.0	30.0	41.0
34	37.397441701234236	39.0	36.0	41.0	33.0	41.0
35	37.56414606962926	39.0	37.0	41.0	33.0	41.0
36	37.72069632015518	40.0	37.0	41.0	33.0	41.0
37	37.84267358064014	40.0	37.0	41.0	33.0	41.0
38	37.85771312612537	40.0	37.0	41.0	33.0	41.0
39	37.88830828533366	40.0	37.0	41.0	33.0	41.0
40	37.890682805394334	40.0	37.0	41.0	33.0	41.0
41	37.83365354545492	40.0	37.0	41.0	33.0	41.0
42	37.73984044040762	40.0	37.0	41.0	33.0	41.0
43	37.72976732844451	40.0	37.0	41.0	33.0	41.0
44	37.669667834455886	40.0	37.0	41.0	33.0	41.0
45	37.638130361647285	40.0	37.0	41.0	33.0	41.0
46	37.58883248171512	40.0	36.0	41.0	33.0	41.0
47	37.49589528746764	40.0	36.0	41.0	33.0	41.0
48	37.50624310313236	40.0	36.0	41.0	33.0	41.0
49	37.49400955814585	40.0	36.0	41.0	33.0	41.0
50	37.31279300924542	39.0	36.0	41.0	32.0	41.0
51	36.67088099433372	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	23.0
11	18.0
12	36.0
13	37.0
14	42.0
15	70.0
16	125.0
17	206.0
18	408.0
19	689.0
20	1302.0
21	2140.0
22	3474.0
23	5853.0
24	8806.0
25	12115.0
26	16080.0
27	20456.0
28	25838.0
29	34500.0
30	46446.0
31	64242.0
32	94309.0
33	120375.0
34	199643.0
35	300014.0
36	437140.0
37	426998.0
38	676756.0
39	1130304.0
40	908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.87940039097749	22.574279522726428	27.037704942875685	10.508615143420405
2	32.0650306596333	24.5144811833475	25.87353435105039	17.546953805968812
3	30.619874275527536	24.193185309275865	27.706472068805425	17.480468346391174
4	27.389116277916386	26.45471039699782	27.240412579059974	18.91576074602582
5	22.031429740464244	38.971114781786895	21.994618893387685	17.002836584361177
6	26.549878560023586	33.697685407777946	24.542915909532383	15.209520122666087
7	81.87129153446952	5.28569047202472	7.941774938591186	4.9012430549145645
8	82.79467620368852	4.055861011499257	7.6654731612830345	5.483989623529185
9	76.47153152135428	6.912173341617612	10.174369345601779	6.441925791426324
10	36.19881163784849	31.196366306502654	18.178193706061528	14.426628349587325
11	23.444652163670504	25.91764675087791	29.431704995226436	21.20599609022515
12	21.442979694795095	23.21323427100884	33.333737444428984	22.01004858976708
13	22.226202104224843	24.510293122901665	34.631512675082284	18.631992097791212
14	20.11098360181464	27.786293194539542	31.422494017548523	20.680229186097293
15	19.011865712046045	27.918768159168337	33.213220494494216	19.856145634291398
16	21.100605753348038	27.632519738301326	30.384323428478538	20.8825510798721
17	21.21354562023935	26.836374958153836	28.857334547503488	23.09274487410332
18	20.881917360199374	26.247621829162952	31.54998188388327	21.320478926754404
19	20.36044872863435	28.740482150458828	29.383955595538065	21.51511352536876
20	21.20293770397852	29.012237677940906	29.329924105180933	20.45490051289964
21	21.278625875325297	28.074525433512477	31.221604881294663	19.425243809867567
22	20.711749851558057	25.513498917854776	28.881140364774556	24.893610865812615
23	18.76983990312355	27.884216660490196	29.498603750242815	23.847339686143442
24	20.233594581972326	26.345379976938116	30.313925438747553	23.10710000234201
25	19.6726975655521	29.093684432400707	27.51621840184165	23.717399600205546
26	20.315785268221852	28.227940700370453	28.99025036060027	22.466023670807427
27	21.41677676397937	27.13788775722475	29.589170557384005	21.85616492141187
28	18.75030480538607	26.856295798300806	31.171761451383368	23.22163794492976
29	21.19296350739041	24.649298155462457	30.253804728926408	23.903933608220722
30	22.613707907581627	25.40088968731445	30.517239241575318	21.468163163528605
31	23.04670376222838	27.060491298064537	27.780011103870788	22.112793835836296
32	20.738421183871008	25.902850774171238	30.703387507181006	22.655340534776744
33	21.480892663041608	24.917747319434667	29.923003059763897	23.678356957759828
34	20.646421619208873	23.387865370388482	30.8284782599711	25.137234750431546
35	20.828381824368726	23.28148312445841	31.591834935312374	24.29830011586049
36	21.90033242729789	23.975240847916922	30.92243408970991	23.201992635075282
37	18.758240077809756	24.666684116918525	33.346026095474	23.22904970979772
38	19.25419460429028	25.16349278730577	32.37500223868363	23.207310369720325
39	22.333190516798393	23.334274728499338	29.44898074456551	24.88355401013676
40	22.17870068179971	22.999064574656998	30.62494403290934	24.197290710633954
41	19.91003935950228	24.457584177948487	29.1983859435466	26.433990519002638
42	20.850231376563116	23.55246716712152	31.03815681255536	24.559144643759996
43	20.94790686525053	22.997356286843566	32.368224193488395	23.68651265441751
44	20.63338903637413	23.586577817331683	30.105982727005966	25.674050419288225
45	22.007348392900685	22.22190383166201	29.993952110079864	25.776795665357437
46	23.709987835337586	22.416951725715105	30.174782641040515	23.698277797906798
47	19.540112388806303	23.07260360972236	32.84607637975238	24.54120762171895
48	19.164206410763317	22.137233372780088	32.89140111286685	25.807159103589743
49	21.53550276701296	20.83819070278134	33.18131408662397	24.44499244358173
50	20.514056866697068	20.698414185401578	32.685552431348185	26.101976516553172
51	19.858763172070045	20.577015538530848	29.843319699176025	29.720901590223082
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1079.0
1	1542.5
2	2006.0
3	3747.0
4	5488.0
5	4381.5
6	3275.0
7	3293.5
8	3312.0
9	3724.0
10	4136.0
11	4658.5
12	5181.0
13	5693.5
14	6206.0
15	6112.5
16	6019.0
17	6406.0
18	6793.0
19	7178.5
20	7564.0
21	8333.0
22	9102.0
23	11520.0
24	13938.0
25	16859.5
26	25418.0
27	31055.0
28	37051.0
29	43047.0
30	50603.5
31	58160.0
32	67611.5
33	77063.0
34	88437.0
35	99811.0
36	111757.5
37	123704.0
38	135621.0
39	147538.0
40	165981.5
41	184425.0
42	206876.5
43	229328.0
44	263438.5
45	297549.0
46	345200.0
47	392851.0
48	439174.5
49	485498.0
50	459424.5
51	433351.0
52	364174.0
53	294997.0
54	246994.0
55	198991.0
56	168181.5
57	137372.0
58	118424.0
59	99476.0
60	87410.0
61	75344.0
62	63368.5
63	51393.0
64	41915.0
65	32437.0
66	25223.0
67	18009.0
68	14205.5
69	10402.0
70	8492.5
71	6583.0
72	4982.0
73	3381.0
74	2513.0
75	1312.5
76	980.0
77	790.5
78	601.0
79	459.0
80	317.0
81	183.5
82	50.0
83	64.0
84	78.0
85	60.0
86	42.0
87	24.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3629365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.834457847075896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.83363556573715	16.491073672486415
2	14.632896291039987	7.560658848230116
3	6.51404218526384	5.048602447478184
4	3.5223381471840525	3.6399078554629525
5	2.219052644886281	2.8664011007378454
6	1.4311745932977782	2.2184171821414465
7	0.9513561712998495	1.7204439633501034
8	0.6838018550344934	1.4132520159712738
9	0.5488870727969678	1.2762179950480332
>10	4.230243309194132	25.086942479569498
>50	1.2659706550259833	22.46567901698513
>100	0.15542984485307018	6.082519002092345
>500	0.006749547233440849	1.2377928321956229
>1k	0.004073002640865761	1.9226495483464767
>5k	0.0	0.0
>10k+	3.4911451207420805E-4	0.9694420399046235
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	11827	0.3258696769269555	Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	10250	0.28241854980141157	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	10169	0.28018675443224916	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	4714	0.1298849798794004	No Hit
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	4312	0.11880866212133527	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3403625703118865	0.0
2	0.0	0.0	0.0	1.1566210618110881	0.0
3	2.75530292489182E-5	0.0	0.0	1.7368327517348077	0.0
4	2.75530292489182E-5	0.0	0.0	2.494568609109307	0.0
5	2.75530292489182E-5	0.0	0.0	4.34359729594571	0.0
6	2.75530292489182E-5	0.0	0.0	5.660136139517519	0.0
7	2.75530292489182E-5	0.0	0.0	6.7728376727058315	0.0
8	2.75530292489182E-5	0.0	0.0	8.791537913657072	0.0
9	2.75530292489182E-5	0.0	0.0	9.496923015458627	0.0
10	2.75530292489182E-5	0.0	0.0	10.947259369063183	0.0
11	2.75530292489182E-5	0.0	0.0	13.335776368593404	0.0
12	2.75530292489182E-5	0.0	0.0	15.216931887534045	0.0
13	2.75530292489182E-5	0.0	0.0	15.946811632337889	0.0
14	2.75530292489182E-5	0.0	0.0	16.22462882625473	0.0
15	5.51060584978364E-5	0.0	0.0	16.71471455750524	0.0
16	5.51060584978364E-5	0.0	0.0	17.749413464889862	0.0
17	5.51060584978364E-5	0.0	0.0	19.118606147356356	0.0
18	5.51060584978364E-5	0.0	0.0	20.58682441694346	0.0
19	8.26590877467546E-5	0.0	0.0	21.534869047340237	0.0
20	8.26590877467546E-5	0.0	0.0	22.338728675677427	0.0
21	8.26590877467546E-5	0.0	0.0	23.282888328950104	0.0
22	1.102121169956728E-4	0.0	0.0	24.331225985812946	0.0
23	1.102121169956728E-4	0.0	0.0	25.37127018087186	0.0
24	1.102121169956728E-4	0.0	0.0	26.155126309974335	0.0
25	1.102121169956728E-4	0.0	0.0	26.85921641940119	0.0
26	1.102121169956728E-4	0.0	0.0	27.49213705427809	0.0
27	1.102121169956728E-4	0.0	0.0	28.131312226794496	0.0
28	1.37765146244591E-4	0.0	0.0	28.79032558037012	0.0
29	1.37765146244591E-4	0.0	0.0	29.508247310479934	0.0
30	1.653181754935092E-4	0.0	0.0	30.23779641893279	0.0
31	1.653181754935092E-4	0.0	0.0	30.92893660461265	0.0
32	1.653181754935092E-4	0.0	0.0	31.701055143255086	0.0
33	1.653181754935092E-4	0.0	0.0	32.4431408800162	0.0
34	2.204242339913456E-4	0.0	0.0	33.15436722401853	0.0
35	2.479772632402638E-4	0.0	0.0	33.919266868997745	0.0
36	2.479772632402638E-4	0.0	0.0	34.649256825918584	0.0
37	2.75530292489182E-4	0.0	0.0	35.38208474485206	0.0
38	2.75530292489182E-4	0.0	0.0	36.12406026949618	0.0
39	2.75530292489182E-4	0.0	0.0	36.83233843936887	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTC	20	7.035301E-4	45.0	27
TAAGCGA	60	3.6379788E-12	41.249996	16
CGTAAGG	670	0.0	40.970146	2
GCGCGTT	485	0.0	40.824738	31
CTCGACG	50	1.0822987E-9	40.5	1
CCCGTTG	40	3.460973E-7	39.375	17
TATAGCG	160	0.0	39.375	1
TTCGCGC	40	3.460973E-7	39.375	1
CGTTTTT	2800	0.0	38.491074	1
CACGCGT	170	0.0	38.382355	31
ACGCGTA	460	0.0	38.152172	25
TACGGGA	2165	0.0	38.140877	4
TACTACG	130	0.0	38.076927	1
TACGGGT	450	0.0	38.0	4
AACGCGT	320	0.0	37.96875	31
CGTACCG	30	1.1404074E-4	37.499996	2
TTTACGG	385	0.0	37.4026	2
TCGCGTT	350	0.0	37.285717	31
GAGTACG	170	0.0	37.058826	1
CGCGTAT	480	0.0	37.031246	26
>>END_MODULE