SRR2935087.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935087.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1552990
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	7797	0.5020637608741847	RNA PCR Primer, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	6911	0.44501252422745796	RNA PCR Primer, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	5685	0.3660680364973374	RNA PCR Primer, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2320	0.14938924268668827	RNA PCR Primer, Index 14 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1958	0.1260793694743688	RNA PCR Primer, Index 14 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1818	0.11706450138120658	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	1646	0.10598909200960727	No Hit
TGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCTT	1617	0.10412172647602366	RNA PCR Primer, Index 14 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTT	20	7.034052E-4	45.000004	26
TGCGAAC	20	7.034052E-4	45.000004	32
GTCGTAC	20	7.034052E-4	45.000004	43
ACGCGCG	20	7.034052E-4	45.000004	1
ATATCCG	25	3.891441E-5	45.0	17
CGGTCTA	35	1.2123019E-7	45.0	31
ATACGCG	30	2.1659962E-6	44.999996	1
ACGTAAG	85	0.0	42.352943	1
CGGGTAC	120	0.0	41.249996	6
CGTATGG	165	0.0	40.90909	2
TCGTATA	95	0.0	40.263157	29
GTCGATG	45	1.9290383E-8	40.0	1
TCGATGG	165	0.0	39.545452	2
GCGTATG	120	0.0	39.374996	1
TGCGTAG	75	0.0	39.0	1
TCGCTCA	215	0.0	38.720932	31
CCTACGG	35	6.2497074E-6	38.571426	2
CGGATCG	35	6.2497074E-6	38.571426	31
CACTACG	35	6.2497074E-6	38.571426	1
AACGCTA	35	6.2497074E-6	38.571426	18