##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935087.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1552990
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00321766399011	33.0	31.0	34.0	30.0	34.0
2	32.321098010933746	34.0	31.0	34.0	30.0	34.0
3	32.37696314850707	34.0	31.0	34.0	30.0	34.0
4	35.90423312448889	37.0	35.0	37.0	35.0	37.0
5	30.3932761962408	37.0	35.0	37.0	0.0	37.0
6	32.86911119839793	37.0	35.0	37.0	17.0	37.0
7	35.04915163652052	37.0	35.0	37.0	32.0	37.0
8	35.588335404606596	37.0	35.0	37.0	35.0	37.0
9	37.43647801981983	39.0	37.0	39.0	35.0	39.0
10	37.38363608265346	39.0	37.0	39.0	34.0	39.0
11	37.294670281199494	39.0	37.0	39.0	34.0	39.0
12	37.1923502405038	39.0	37.0	39.0	34.0	39.0
13	37.16452198661936	39.0	37.0	39.0	34.0	39.0
14	38.353755658439525	40.0	38.0	41.0	34.0	41.0
15	38.44999195101063	40.0	38.0	41.0	34.0	41.0
16	38.52029182415856	40.0	38.0	41.0	34.0	41.0
17	38.51168455688704	40.0	38.0	41.0	34.0	41.0
18	38.46348849638439	40.0	38.0	41.0	34.0	41.0
19	38.46335906863534	40.0	38.0	41.0	34.0	41.0
20	38.386306415366484	40.0	37.0	41.0	34.0	41.0
21	38.33553274650835	40.0	37.0	41.0	34.0	41.0
22	38.324988570435096	40.0	37.0	41.0	34.0	41.0
23	38.2626262886432	40.0	37.0	41.0	34.0	41.0
24	38.24107753430479	40.0	37.0	41.0	34.0	41.0
25	38.18534375623797	40.0	37.0	41.0	34.0	41.0
26	38.11694151282365	40.0	37.0	41.0	34.0	41.0
27	38.07934307368367	40.0	37.0	41.0	34.0	41.0
28	38.02942581729438	40.0	37.0	41.0	34.0	41.0
29	38.02801692219525	40.0	37.0	41.0	34.0	41.0
30	37.869285056568295	40.0	36.0	41.0	33.0	41.0
31	33.248338366634684	39.0	33.0	41.0	9.0	41.0
32	34.19570248359616	39.0	33.0	41.0	16.0	41.0
33	36.12893515090245	39.0	34.0	41.0	30.0	41.0
34	36.940161881274186	39.0	35.0	41.0	31.0	41.0
35	37.12938975782201	39.0	35.0	41.0	32.0	41.0
36	37.28682090676695	39.0	35.0	41.0	33.0	41.0
37	37.369157560576696	39.0	35.0	41.0	33.0	41.0
38	37.37051494214386	39.0	35.0	41.0	33.0	41.0
39	37.396473254818126	39.0	35.0	41.0	33.0	41.0
40	37.325488895614264	39.0	35.0	41.0	33.0	41.0
41	37.282351463950185	39.0	35.0	41.0	33.0	41.0
42	37.2479275462173	39.0	35.0	41.0	33.0	41.0
43	37.25794499642625	39.0	35.0	41.0	33.0	41.0
44	37.19050927565535	39.0	35.0	41.0	33.0	41.0
45	37.16223736147689	39.0	35.0	41.0	33.0	41.0
46	37.08414091526668	39.0	35.0	41.0	32.0	41.0
47	36.99465804673565	39.0	35.0	41.0	32.0	41.0
48	37.013097959420215	39.0	35.0	41.0	32.0	41.0
49	36.99552476191089	39.0	35.0	41.0	32.0	41.0
50	36.81927250014488	39.0	35.0	41.0	32.0	41.0
51	36.23025389732065	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	16.0
11	17.0
12	7.0
13	20.0
14	38.0
15	51.0
16	90.0
17	141.0
18	252.0
19	488.0
20	798.0
21	1275.0
22	2170.0
23	3397.0
24	4893.0
25	6341.0
26	7955.0
27	9913.0
28	12491.0
29	16170.0
30	21320.0
31	30259.0
32	46330.0
33	56122.0
34	100689.0
35	147855.0
36	183787.0
37	186705.0
38	284662.0
39	428325.0
40	403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25816006542218	21.997244026040093	24.4067250915975	11.337870816940224
2	30.565489797101076	24.925530750358984	25.899716031655068	18.609263420884876
3	31.42666726765787	24.812200980044945	26.03262094411426	17.728510808182925
4	28.012801112692294	26.765272152428544	26.41530209466899	18.806624640210178
5	22.762413151404708	39.43901763694551	20.419706501651653	17.37886270999813
6	26.484909754731195	33.88540814815292	23.48225036864371	16.14743172847217
7	77.31987971590287	7.0185899458464	9.467865214843624	6.193665123407105
8	77.04608529353054	6.473061642380183	9.372693964545812	7.108159099543461
9	71.13735439378232	8.318147573390686	12.12506197721814	8.419436055608857
10	38.61177470556797	25.33828292519591	19.63992041159312	16.410021957642996
11	28.13501696726959	25.81021126987296	24.881100329042685	21.17367143381477
12	25.49256595341889	22.771106059923117	30.832136716913823	20.90419126974417
13	23.50858666185874	24.79687570428657	31.48758201920167	20.206955614653026
14	19.164514903508714	28.621433492810645	29.911589900772057	22.302461702908584
15	17.9467350079524	27.06720584163452	33.730352416950524	21.255706733462546
16	20.0138442617145	26.961989452604328	29.897166111822997	23.12700017385817
17	20.53509681324413	26.842800018029738	27.560769869735157	25.061333298990977
18	20.780816360697752	26.953296544085926	30.25982137682793	22.0060657183884
19	22.027443834152184	28.07332951274638	27.54364162035815	22.35558503274329
20	22.997636816721293	28.442166401586615	27.79064900611079	20.769547775581298
21	22.05777242609418	28.637209511973676	28.44435572669496	20.860662335237187
22	20.64623725844983	26.653809747648083	27.045505766295985	25.654447227606102
23	20.380427433531445	27.27596442990618	28.428837275191725	23.914770861370645
24	21.638516667847185	25.246202486815754	29.4247870237413	23.69049382159576
25	20.029555888962584	27.77364954056369	27.11788227870109	25.078912291772642
26	19.01918235146395	27.858324908724462	28.381831177277384	24.740661562534207
27	21.090670255442724	27.2048757558001	27.79844042781988	23.90601356093729
28	18.169080290278753	27.670300517067076	28.774364290819644	25.386254901834526
29	19.328392327059415	25.72824036214013	28.05948525103188	26.883882059768577
30	20.424278327613184	26.935588767474357	26.36733011803038	26.272802786882078
31	22.579346937198565	30.712045795529914	22.922105100483584	23.786502166787937
32	19.749579842754944	28.785890443595903	24.705181617396118	26.75934809625303
33	20.003155203832605	27.21376184006336	23.98830642824487	28.794776527859163
34	18.16341380176305	28.528837919110877	26.575316003322623	26.732432275803454
35	18.68221946052454	27.062376448013186	26.182976065525214	28.072428025937064
36	19.463937308031603	28.555302996155802	25.237380794467445	26.74337890134515
37	18.847706681949013	30.506828762580568	26.211759251508383	24.433705303962032
38	18.18170110560918	29.583963837500566	24.70299229228778	27.531342764602478
39	20.56896696050844	26.016394181546566	25.81375282519527	27.600886032749727
40	20.825182390099098	26.26797339326074	27.946863791782306	24.959980424857857
41	17.81769361039028	28.523171430595173	25.64163323653082	28.017501722483722
42	19.746746598497094	26.319744492881476	27.106613693584634	26.8268952150368
43	20.571027501786876	24.280259370633424	28.07519687827996	27.07351624929974
44	19.872182048821948	25.18651118165603	26.602167431857254	28.339139337664765
45	19.880102254360942	24.349609463035822	26.16610538380801	29.60418289879523
46	20.533873366860057	25.999587891744312	27.146150329364644	26.320388412030987
47	17.646990643854757	26.04768865221283	30.267226447047307	26.03809425688511
48	18.029607402494545	24.47858646868299	29.074108654917293	28.41769747390518
49	20.136510859696457	23.18257039646102	29.830649263678456	26.85026948016407
50	18.725040083967055	22.799309718671722	30.072440904320054	28.403209293041165
51	17.864764100219578	23.080380427433532	27.305649102698666	31.749206369648224
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1248.0
1	1803.5
2	2359.0
3	2840.0
4	3321.0
5	2724.0
6	2127.0
7	2156.0
8	2185.0
9	2298.0
10	2411.0
11	2558.5
12	2706.0
13	2759.5
14	2813.0
15	2936.5
16	3060.0
17	3039.0
18	3018.0
19	3275.5
20	3533.0
21	4031.5
22	4530.0
23	5380.5
24	6231.0
25	8244.5
26	11945.5
27	13633.0
28	16193.0
29	18753.0
30	22985.0
31	27217.0
32	29918.0
33	32619.0
34	34711.0
35	36803.0
36	41477.5
37	46152.0
38	49493.0
39	52834.0
40	59024.5
41	65215.0
42	75594.0
43	85973.0
44	98571.5
45	111170.0
46	129866.5
47	148563.0
48	169278.5
49	189994.0
50	180178.5
51	170363.0
52	144197.0
53	118031.0
54	100849.5
55	83668.0
56	74644.0
57	65620.0
58	61722.0
59	57824.0
60	53830.5
61	49837.0
62	45884.5
63	41932.0
64	35628.0
65	29324.0
66	24963.0
67	20602.0
68	18039.5
69	15477.0
70	12450.5
71	9424.0
72	7373.0
73	5322.0
74	4140.0
75	2467.5
76	1977.0
77	1521.0
78	1065.0
79	812.5
80	560.0
81	363.5
82	167.0
83	105.0
84	43.0
85	50.0
86	57.0
87	35.0
88	13.0
89	6.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1552990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.457409080391553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.48922550941732	16.926434132316423
2	13.718756661059322	6.984880007898673
3	5.877605542204873	4.488858261032581
4	3.214568120099674	3.2733830260065058
5	1.875821649203904	2.387677954281926
6	1.2833854918774736	1.9603001684738621
7	0.8370556197261834	1.4916487134087082
8	0.6120977708848415	1.2465938680488953
9	0.44895509045552373	1.0286310056805414
>10	4.010757836718383	24.718004970670975
>50	1.4861250140541893	25.4025253702449
>100	0.13338979440116694	5.8745171934894005
>500	0.007980585979519818	1.4224455296473733
>1k	0.003420251134079922	1.3144052626791556
>5k	8.550627835199805E-4	1.4796945361201224
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	7797	0.5020637608741847	RNA PCR Primer, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	6911	0.44501252422745796	RNA PCR Primer, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	5685	0.3660680364973374	RNA PCR Primer, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2320	0.14938924268668827	RNA PCR Primer, Index 14 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1958	0.1260793694743688	RNA PCR Primer, Index 14 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1818	0.11706450138120658	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	1646	0.10598909200960727	No Hit
TGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCTT	1617	0.10412172647602366	RNA PCR Primer, Index 14 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.575676598046349E-4	0.0	0.0	0.5325855285610339	0.0
2	2.575676598046349E-4	0.0	0.0	1.6918331734267444	0.0
3	2.575676598046349E-4	0.0	0.0	2.3598348991300653	0.0
4	2.575676598046349E-4	0.0	0.0	3.1668587692129377	0.0
5	2.575676598046349E-4	0.0	0.0	5.155538670564524	0.0
6	2.575676598046349E-4	0.0	0.0	6.536165719032319	0.0
7	2.575676598046349E-4	0.0	0.0	7.7188520209402505	0.0
8	2.575676598046349E-4	0.0	0.0	9.738504433383344	0.0
9	2.575676598046349E-4	0.0	0.0	10.44791016040026	0.0
10	2.575676598046349E-4	0.0	0.0	11.939742045988705	0.0
11	2.575676598046349E-4	0.0	0.0	14.195841570132455	0.0
12	2.575676598046349E-4	0.0	0.0	15.96333524362681	0.0
13	2.575676598046349E-4	0.0	0.0	16.71086098429481	0.0
14	2.575676598046349E-4	0.0	0.0	17.005067643706656	0.0
15	2.575676598046349E-4	0.0	0.0	17.598181572321778	0.0
16	3.2195957475579366E-4	0.0	0.0	18.659811074121535	0.0
17	3.2195957475579366E-4	0.0	0.0	19.92292287780346	0.0
18	3.863514897069524E-4	0.0	0.0	21.372900018673654	0.0
19	3.863514897069524E-4	0.0	0.0	22.18784409429552	0.0
20	3.863514897069524E-4	0.0	0.0	22.949471664337825	0.0
21	3.863514897069524E-4	0.0	0.0	23.813675554897326	0.0
22	3.863514897069524E-4	0.0	0.0	24.667061603745033	0.0
23	3.863514897069524E-4	0.0	0.0	25.49527041384684	0.0
24	3.863514897069524E-4	0.0	0.0	26.180722348501924	0.0
25	3.863514897069524E-4	0.0	0.0	26.78864641755581	0.0
26	3.863514897069524E-4	0.0	0.0	27.34763263124682	0.0
27	3.863514897069524E-4	0.0	0.0	27.85877565212912	0.0
28	4.5074340465811114E-4	0.0	0.0	28.397671588355365	0.0
29	4.5074340465811114E-4	0.0	0.0	28.95034739438116	0.0
30	4.5074340465811114E-4	0.0	0.0	29.558400247264952	0.0
31	4.5074340465811114E-4	0.0	0.0	30.120219705213813	0.0
32	4.5074340465811114E-4	0.0	0.0	30.750294593010903	0.0
33	4.5074340465811114E-4	0.0	0.0	31.348495482907165	0.0
34	4.5074340465811114E-4	0.0	0.0	31.951976509829425	0.0
35	4.5074340465811114E-4	0.0	0.0	32.59911525508857	0.0
36	4.5074340465811114E-4	0.0	0.0	33.211675542018945	0.0
37	4.5074340465811114E-4	0.0	0.0	33.795710210625955	0.0
38	4.5074340465811114E-4	0.0	0.0	34.38393035370479	0.0
39	4.5074340465811114E-4	0.0	0.0	34.96867333337626	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTT	20	7.034052E-4	45.000004	26
TGCGAAC	20	7.034052E-4	45.000004	32
GTCGTAC	20	7.034052E-4	45.000004	43
ACGCGCG	20	7.034052E-4	45.000004	1
ATATCCG	25	3.891441E-5	45.0	17
CGGTCTA	35	1.2123019E-7	45.0	31
ATACGCG	30	2.1659962E-6	44.999996	1
ACGTAAG	85	0.0	42.352943	1
CGGGTAC	120	0.0	41.249996	6
CGTATGG	165	0.0	40.90909	2
TCGTATA	95	0.0	40.263157	29
GTCGATG	45	1.9290383E-8	40.0	1
TCGATGG	165	0.0	39.545452	2
GCGTATG	120	0.0	39.374996	1
TGCGTAG	75	0.0	39.0	1
TCGCTCA	215	0.0	38.720932	31
CCTACGG	35	6.2497074E-6	38.571426	2
CGGATCG	35	6.2497074E-6	38.571426	31
CACTACG	35	6.2497074E-6	38.571426	1
AACGCTA	35	6.2497074E-6	38.571426	18
>>END_MODULE