FastQCFastQC Report
Sat 14 Jan 2017
SRR2935086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673609
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT75100.2808937282901127Illumina PCR Primer Index 8 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC65340.2443887644004789RNA PCR Primer, Index 40 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC53110.1986453516576283Illumina PCR Primer Index 8 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC33320.12462555295108596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA2200.039.8863631
TAGTACG1750.039.8571431
TTATGCG1150.039.1304361
TATAGCG1150.039.1304361
ACCGGTA750.039.041
GCGATAT1450.038.7931029
CCCTCGT6800.038.71323414
ACGTCGA356.2513973E-638.5714321
CGTGTAC6900.038.4782618
CGTTAGG3400.038.382352
TAAGGGA19500.038.3076934
AGTACGG4850.038.0412332
TGTTACG1600.037.9687541
CGTAAGG4700.037.819152
CGAACTA301.14032686E-437.5000046
AAGGGCG14450.037.3702435
TATTGCG1900.036.7105261
TATGGGA15450.036.699034
TACGGGA12150.036.481484
ACGGGTA3950.036.4556965
Produced by FastQC (version 0.11.5)