##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2673609
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.066245662697874	33.0	31.0	34.0	30.0	34.0
2	32.38064129796092	34.0	31.0	34.0	30.0	34.0
3	32.44179496702771	34.0	31.0	34.0	30.0	34.0
4	35.94334137863839	37.0	35.0	37.0	35.0	37.0
5	30.40683585370935	37.0	35.0	37.0	0.0	37.0
6	32.895615626667926	37.0	35.0	37.0	17.0	37.0
7	35.08843589320652	37.0	35.0	37.0	32.0	37.0
8	35.63334130009287	37.0	35.0	37.0	35.0	37.0
9	37.50808850508807	39.0	37.0	39.0	35.0	39.0
10	37.425445530741406	39.0	37.0	39.0	35.0	39.0
11	37.31506701241655	39.0	37.0	39.0	34.0	39.0
12	37.19540665819123	39.0	37.0	39.0	34.0	39.0
13	37.186971617764605	39.0	37.0	39.0	34.0	39.0
14	38.41616406886721	40.0	38.0	41.0	34.0	41.0
15	38.50658342338016	40.0	38.0	41.0	34.0	41.0
16	38.55468694188268	40.0	38.0	41.0	34.0	41.0
17	38.543451940803614	40.0	38.0	41.0	34.0	41.0
18	38.51189160419493	40.0	38.0	41.0	34.0	41.0
19	38.50491676232389	40.0	38.0	41.0	34.0	41.0
20	38.430280194299165	40.0	38.0	41.0	34.0	41.0
21	38.390609846091934	40.0	37.0	41.0	34.0	41.0
22	38.37293785291716	40.0	37.0	41.0	34.0	41.0
23	38.31605444176766	40.0	37.0	41.0	34.0	41.0
24	38.31032248918971	40.0	37.0	41.0	34.0	41.0
25	38.21692251933622	40.0	37.0	41.0	34.0	41.0
26	38.139187517696115	40.0	37.0	41.0	34.0	41.0
27	38.07604627303394	40.0	37.0	41.0	34.0	41.0
28	37.99811116733973	40.0	37.0	41.0	34.0	41.0
29	38.00209454710842	40.0	37.0	41.0	34.0	41.0
30	37.861306571005706	40.0	36.0	41.0	33.0	41.0
31	33.28086866853007	39.0	33.0	41.0	9.0	41.0
32	34.20940608742715	39.0	33.0	41.0	16.0	41.0
33	36.129146408468856	39.0	34.0	41.0	30.0	41.0
34	36.98691992733418	39.0	35.0	41.0	31.0	41.0
35	37.18041269310509	39.0	35.0	41.0	32.0	41.0
36	37.302240903587624	39.0	35.0	41.0	32.0	41.0
37	37.41021443300049	39.0	35.0	41.0	33.0	41.0
38	37.40129278439742	40.0	35.0	41.0	33.0	41.0
39	37.403491310808725	40.0	35.0	41.0	33.0	41.0
40	37.35496364651675	39.0	35.0	41.0	33.0	41.0
41	37.29930143113671	39.0	35.0	41.0	33.0	41.0
42	37.206093336759416	39.0	35.0	41.0	32.0	41.0
43	37.18235426346934	39.0	35.0	41.0	32.0	41.0
44	37.103450429737485	39.0	35.0	41.0	32.0	41.0
45	37.03911155296081	39.0	35.0	41.0	32.0	41.0
46	36.96096063410918	39.0	35.0	41.0	32.0	41.0
47	36.864209388882216	39.0	35.0	41.0	32.0	41.0
48	36.85127107217248	39.0	35.0	41.0	32.0	41.0
49	36.80528977872232	39.0	35.0	41.0	32.0	41.0
50	36.583871089602106	39.0	35.0	41.0	31.0	41.0
51	35.920495480079545	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	26.0
11	25.0
12	21.0
13	37.0
14	49.0
15	85.0
16	127.0
17	238.0
18	431.0
19	698.0
20	1267.0
21	2069.0
22	3452.0
23	5535.0
24	8050.0
25	11057.0
26	13972.0
27	17307.0
28	22144.0
29	28788.0
30	37890.0
31	52861.0
32	81180.0
33	97231.0
34	173887.0
35	253056.0
36	312739.0
37	326796.0
38	475152.0
39	746629.0
40	798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34265369393954	21.322265147970402	25.531706393866866	10.803374764223191
2	31.983584735090286	24.613771123601094	25.29700490984284	18.105639231465783
3	32.85861919226035	23.418757192992693	25.88291706079685	17.839706553950112
4	28.74234789006171	26.49531027162162	25.545470560579354	19.216871277737322
5	22.48271905128985	40.26332945468092	20.41229663724202	16.841654856787212
6	27.95072129095915	34.20148570714716	22.38689352107956	15.460899480814136
7	80.59338519581584	6.557652970198709	7.741371307472409	5.107590526513039
8	81.0400099640598	5.725556728751287	7.575565462264677	5.65886784492422
9	74.57062719343031	7.52537113691643	10.304872552418846	7.59912911723442
10	40.52888810592723	25.21722510658814	17.785248329131147	16.468638458353485
11	30.47831601404693	26.037614325804558	23.52344714578684	19.960622514361674
12	26.73812812569078	23.708664954374406	28.892594242464025	20.660612677470787
13	25.318698433465777	23.8410702537282	30.526565402794496	20.313665910011526
14	20.98324773742159	27.080885798933203	29.424945831645537	22.510920631999667
15	21.215518050694772	26.651915070603067	31.40881856696323	20.723748311738927
16	23.49973388030935	26.019997688517655	28.916270105314577	21.563998325858417
17	22.83093750806494	25.96329530608253	27.532485116559673	23.673282069292856
18	23.4860071162238	25.363955612058458	29.05368735667781	22.096349915039934
19	23.814140362334207	27.22840175956918	27.16309677293875	21.794361105157858
20	24.57621888615725	27.694101867550565	27.616192195642668	20.113487050649514
21	24.458625027070152	26.961459211126233	27.902883331107876	20.67703243069574
22	22.292302277558164	25.41512240570704	27.79228376325783	24.500291553476966
23	22.04765169476913	26.44208633349155	28.20087005990779	23.309391911831536
24	22.890295477012533	25.10872008584651	28.36832162070071	23.63266281644025
25	22.615273961151388	26.778896989051127	26.469315445901024	24.136513603896457
26	21.947898888730553	27.74571749272238	27.188979390778535	23.117404227768535
27	22.542787670149224	27.150230269272736	27.510006137771082	22.79697592280696
28	20.891686106682016	27.61413505116118	27.757536722834192	23.73664211932261
29	23.181549732963944	26.643649090050193	26.369861860877936	23.80493931610793
30	22.998987510888842	28.898316844385246	25.7358499316841	22.366845713041812
31	25.262108258911457	30.13716665376276	23.006505438902998	21.59421964842279
32	25.09761150564649	28.902992172752263	23.799852558844616	22.199543762756633
33	23.371368064664654	29.45628923301799	24.162171805974623	23.010170896342732
34	22.094217965304573	29.103058824233464	25.49303207761494	23.309691132847025
35	24.720929649773023	27.36967148150683	25.311255310705494	22.59814355801465
36	22.588979914415308	30.424007399735714	24.553926920503336	22.43308576534564
37	22.913260689951297	30.568231929201318	25.413813313764276	21.10469406708311
38	23.29274774284497	29.104891552953333	24.90510018480638	22.697260519395318
39	23.69624728223162	26.73236812114262	26.040756146467192	23.53062845015857
40	23.341819989385133	26.92521606562515	27.591768280253394	22.14119566473632
41	21.499366586512835	27.648171441673036	25.953907246721563	24.898554725092563
42	22.996481534884122	26.484800133452573	26.22339317379617	24.295325157867136
43	23.072521075445213	25.497557795474208	27.285852194543033	24.14406893453755
44	22.323383860542062	26.528897830610237	26.02302730129948	25.124691007548222
45	23.11676838311062	26.08279669914337	25.838445337369826	24.961989580376187
46	23.645978151629503	27.193280692876183	25.95559036493369	23.205150790560623
47	20.99110228907817	27.2205472079126	28.36753616553505	23.420814337474177
48	21.481375922956573	25.500550005629098	27.87168954024317	25.14638453117116
49	22.809692815965235	24.751674609114495	28.64798106230193	23.79065151261834
50	21.546905325348618	25.067315377828248	28.55668124995091	24.829098046872225
51	21.588197825486073	24.56107082224813	26.260346969209035	27.590384383056758
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1178.0
1	2127.0
2	3076.0
3	4291.0
4	5506.0
5	4471.0
6	3436.0
7	3368.5
8	3301.0
9	3474.0
10	3647.0
11	3928.0
12	4209.0
13	4338.0
14	4467.0
15	4536.0
16	4605.0
17	4687.0
18	4769.0
19	5099.5
20	5430.0
21	6706.5
22	7983.0
23	9376.0
24	10769.0
25	12929.0
26	19704.5
27	24320.0
28	28218.5
29	32117.0
30	37847.5
31	43578.0
32	47551.0
33	51524.0
34	59278.0
35	67032.0
36	70959.0
37	74886.0
38	84476.0
39	94066.0
40	103990.5
41	113915.0
42	127925.5
43	141936.0
44	160392.0
45	178848.0
46	205320.0
47	231792.0
48	260459.5
49	289127.0
50	281145.5
51	273164.0
52	239235.0
53	205306.0
54	183260.5
55	161215.0
56	145348.5
57	129482.0
58	123580.0
59	117678.0
60	110570.0
61	103462.0
62	94231.5
63	85001.0
64	73571.5
65	62142.0
66	51729.0
67	41316.0
68	34032.5
69	26749.0
70	22490.0
71	18231.0
72	15839.0
73	13447.0
74	10382.0
75	6075.5
76	4834.0
77	3384.0
78	1934.0
79	1474.0
80	1014.0
81	744.5
82	475.0
83	338.0
84	201.0
85	112.0
86	23.0
87	17.0
88	11.0
89	5.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2673609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.859687841310702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.45349636350575	15.855596791969232
2	13.494586200111472	6.439532285646377
3	5.889835555409893	4.215889133661987
4	3.1837942970948117	3.038573523185097
5	1.850758596470991	2.2079261190710078
6	1.203711456483406	1.7232107761622113
7	0.7995688722460746	1.3354224589594126
8	0.6127745788852602	1.169648813543434
9	0.4458647813617159	0.957437505245231
>10	4.086062311676076	23.858973032989734
>50	1.7756201786485275	29.17443801688937
>100	0.194577578326937	6.934762090794664
>500	0.005115616294234593	0.8244740440282058
>1k	0.003704411799273326	1.4494901017043942
>5k	5.292016856104753E-4	0.8146253061496719
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	7510	0.2808937282901127	Illumina PCR Primer Index 8 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	6534	0.2443887644004789	RNA PCR Primer, Index 40 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	5311	0.1986453516576283	Illumina PCR Primer Index 8 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3332	0.12462555295108596	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.740262693610023E-5	0.0	0.0	0.30011867853526825	0.0
2	3.740262693610023E-5	0.0	0.0	0.9306147607971098	0.0
3	3.740262693610023E-5	0.0	0.0	1.3233049409992261	0.0
4	3.740262693610023E-5	0.0	0.0	1.8507193834251754	0.0
5	3.740262693610023E-5	0.0	0.0	3.1407359864512725	0.0
6	3.740262693610023E-5	0.0	0.0	4.09659752043025	0.0
7	3.740262693610023E-5	0.0	0.0	4.887251651232472	0.0
8	3.740262693610023E-5	0.0	0.0	6.339782668295925	0.0
9	3.740262693610023E-5	0.0	0.0	6.840828258731924	0.0
10	3.740262693610023E-5	0.0	0.0	7.9236343085320255	0.0
11	3.740262693610023E-5	0.0	0.0	9.619319803307066	0.0
12	3.740262693610023E-5	0.0	0.0	10.994389979985854	0.0
13	3.740262693610023E-5	0.0	0.0	11.521467798769379	0.0
14	3.740262693610023E-5	0.0	0.0	11.746818626059383	0.0
15	7.480525387220046E-5	0.0	0.0	12.210162368543791	0.0
16	7.480525387220046E-5	0.0	0.0	13.108648272802792	0.0
17	7.480525387220046E-5	0.0	0.0	14.14948857518059	0.0
18	7.480525387220046E-5	0.0	0.0	15.24497411551203	0.0
19	7.480525387220046E-5	0.0	0.0	15.962244292265623	0.0
20	1.496105077444009E-4	0.0	0.0	16.614433898150402	0.0
21	1.496105077444009E-4	0.0	0.0	17.3592698109559	0.0
22	1.496105077444009E-4	0.0	0.0	18.155983167321775	0.0
23	1.8701313468050116E-4	0.0	0.0	18.90152973003906	0.0
24	1.8701313468050116E-4	0.0	0.0	19.542161924200585	0.0
25	1.8701313468050116E-4	0.0	0.0	20.139294863235424	0.0
26	2.2441576161660138E-4	0.0	0.0	20.64583863983103	0.0
27	2.2441576161660138E-4	0.0	0.0	21.150699298214512	0.0
28	2.2441576161660138E-4	0.0	0.0	21.658664374633688	0.0
29	2.2441576161660138E-4	0.0	0.0	22.216636763266433	0.0
30	2.2441576161660138E-4	0.0	0.0	22.806625800556475	0.0
31	2.2441576161660138E-4	0.0	0.0	23.349001293756867	0.0
32	2.2441576161660138E-4	0.0	0.0	23.97601893171365	0.0
33	2.2441576161660138E-4	0.0	0.0	24.59922150172295	0.0
34	2.2441576161660138E-4	0.0	0.0	25.22840849204203	0.0
35	2.2441576161660138E-4	0.0	0.0	25.874426664482353	0.0
36	2.2441576161660138E-4	0.0	0.0	26.49740481873004	0.0
37	2.2441576161660138E-4	0.0	0.0	27.108937769135277	0.0
38	2.2441576161660138E-4	0.0	0.0	27.73128007872505	0.0
39	2.2441576161660138E-4	0.0	0.0	28.371800065005765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGAA	220	0.0	39.88636	31
TAGTACG	175	0.0	39.857143	1
TTATGCG	115	0.0	39.130436	1
TATAGCG	115	0.0	39.130436	1
ACCGGTA	75	0.0	39.0	41
GCGATAT	145	0.0	38.793102	9
CCCTCGT	680	0.0	38.713234	14
ACGTCGA	35	6.2513973E-6	38.57143	21
CGTGTAC	690	0.0	38.47826	18
CGTTAGG	340	0.0	38.38235	2
TAAGGGA	1950	0.0	38.307693	4
AGTACGG	485	0.0	38.041233	2
TGTTACG	160	0.0	37.968754	1
CGTAAGG	470	0.0	37.81915	2
CGAACTA	30	1.14032686E-4	37.500004	6
AAGGGCG	1445	0.0	37.370243	5
TATTGCG	190	0.0	36.710526	1
TATGGGA	1545	0.0	36.69903	4
TACGGGA	1215	0.0	36.48148	4
ACGGGTA	395	0.0	36.455696	5
>>END_MODULE