FastQCFastQC Report
Sat 14 Jan 2017
SRR2935084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3133209
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC66370.2118275544338089TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT65390.20869977074622217Illumina PCR Primer Index 5 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC63930.20404001137491945TruSeq Adapter, Index 23 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG44420.14177158306388116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA406.8212103E-945.031
ATACGTC356.251741E-638.5714332
CAATCGA301.14037175E-437.49999630
ACGGGAT17200.036.758725
CGTAAGG4600.035.706522
TTACGGG12800.035.332033
TGACGGG15950.035.2664573
GTTAGCG2050.035.1219521
TACGGGA12850.034.8443574
TAACGGG13500.034.8333323
TAAGGGA28650.034.7905774
TACGTAG1750.034.7142871
ATAACGG3450.034.565222
TCGAACG850.034.4117661
TATGGGA18750.034.324
AACGAAT4750.034.10526724
CGTATGG3900.034.038462
CGTTATT4050.033.888891
GATACGA606.4392225E-933.759
TCGACGT606.4392225E-933.7526
Produced by FastQC (version 0.11.5)